• Title/Summary/Keyword: Bio-assessment

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The Comparative Assessment of Cold Tolerance of Broad-leaved Evergreen Trees by Low Temperature Treatment (저온처리에 따른 국내 상록활엽수종의 내한성 비교 평가)

  • Jin, Eon-Ju;Yoon, Jun Hyuck;Bae, Eun-Ji;Choi, Myung Suk
    • Journal of Korean Society of Forest Science
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    • v.108 no.4
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    • pp.484-492
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    • 2019
  • The aim of the present study was to compare the cold tolerance of seven different types of trees growing in southern Korea to select evergreen broad-leaved trees that can be used as street trees in large land areas experiencing climate change. The trees compared were the thorn tree, Cinnamomum camphora, Camellia japonica, Machilus thunbergii, Dendropanax morbifera, Daphniphyllum macropodum Miq., Quercus glauca Thunb., and Raphiolepis indica. When the trees were subjected to low temperature treatment, their electrolyte elution volume values appeared to increase with the decreases in the treatment temperature. The analysis of the cold tolerance of each type of tree was based on the estimated temperatures in the following order: C. japonica (-11.586℃) > R. indica (-9.348℃) > Q. glauca (-8.719℃) > M. thunbergii (-8.090℃) > D. macropodum (-7.409℃) > D. morbifera (-7.085℃) > C. camphora (-6.995℃). The relative cold tolerance difference found in the seven tree species was more than 5℃, as evaluated previously. In the Lauraceae family, the difference in cold tolerance was more than 2℃, even in the same species. The analysis showed that trees with excellent cold tolerance included Q. glauca Thunb., C. japonica, R. indica, and the thorn tree. This knowledge is required for the evaluation of the possibility of the survival of trees under cold temperature conditions in cities.

Residual Characteristics and Risk Assessments of Metalaxyl-M and Dinotefuran in Crown Daisy (Metalaxyl-M 및 dinotefuran 입제의 쑥갓 중 잔류 특성 및 위해성 평가)

  • Song, Min-Ho;Yu, Ji-Woo;Kim, Jinchan;Lee, Kwanghun;Ko, Rakdo;Keum, Young-Soo;Lee, Jiho
    • Korean Journal of Environmental Agriculture
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    • v.41 no.2
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    • pp.108-114
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    • 2022
  • BACKGROUND: This study was performed to determine residual characteristics of soil-treated metalaxyl-M and dinotefuran in crown daisy and to evaluate the risks from intake of the residual pesticides in the crop. METHODS AND RESULTS: The pesticide granules were treated in soil on two levels, and the plants samples were collected 51 days after seeding. The analytes were extracted and partitioned using the QuEChERS extraction packet (MgSO4 4 g, NaCl 1 g). The quantitative methods for metalaxyl-M and dinotefuran were validated in linearity, accuracy, and precision. Risk assessments of the pesticides were performed using Korea national nutrition statistics 2019. CONCLUSION(S): The residual concentrations of metalaxyl-M in crown daisy were 0.09-0.10 mg/kg (for the treatment at 6 kg/10 a) and 0.17-0.19 mg/kg (12 kg/10 a), respectively. The residual concentrations of dinotefuran in the crop were 0.53-0.75 mg/kg (3 kg/10 a) and 1.17-1.26 mg/kg (6 kg/10 a). The amounts of pesticides were less than MRL (Maximum Residue Limits) according to the Korean MFDS (Ministry of Food and Drug Safety). The HI (Hazard Index) of metalaxyl-M and dinotefuran for consumers was 0.0075% and 0.2250%, respectively. For females in the age between 50-64, the major consumer group, the HIs of the pesticides were <3%. Considering the consumption of crown daisy, they are not considered to be of toxicological concern.

Detection of single-nucleotide polymorphism in RPB2 of Wolfiporia hoelen strains and assessment of its applicability for strain breeding (복령 균주의 RPB2 유전자 내 단일염기다형성 및 육종 활용성 분석)

  • Su Yeon, Kim;Mi-Jeong, Park;Seong Hwan, Kim;Kang-Hyeon, Ka
    • Journal of Mushroom
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    • v.20 no.4
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    • pp.199-207
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    • 2022
  • The demand for novel strains has been rising in the domestic market to increase the production of sclerotia from Wolfiporia hoelen. To improve strain breeding efficiency, we investigated whether single-nucleotide polymorphisms (SNPs) in the RNA polymerase II subunit (RPB2) gene, which may be linked to the mating type locus, are useful for distinguishing monokaryons from dikaryons in Korean W. hoelen strains. We designed a specific primer set to efficiently amplify a region of RPB2 using PCR with the genomic DNA of 12 cultivated strains and 31 wild strains of W. hoelen collected from Korea. Nucleotide sequences of the PCR-amplified RPB2 genes were determined and analyzed for the presence of SNPs among the 43 W. hoelen strains. Previously reported SNP loci were detected in the RPB2 gene of all W. hoelen strains tested. However, these previously reported SNP loci could not be applied to differentiate monokaryons from dikaryons in approximately one-third of Korean wild strains with homozygous genotypes. Three additional SNPs in the RPB2 gene, which may improve the ability to distinguish monokaryons from dikaryons, were identified by searching through the multiple sequence alignments of the 43 W. hoelen strains. The applicability of these three novel SNPs, together with the previously known SNPs, in the RPB2 gene to W. hoelen strain breeding was verified by examining the hybrid strains and their parental strains.

Toxic effects of phenanthrene on fertilization and normal embryogenesis rates of Mesocentrotus nudus and Hemicentrotus pulcherrimus (둥근성게(Mesocentrotus nudus)와 말똥성게(Hemicentrotus pulcherrimus)의 수정 및 정상 배아발생률을 이용한 Phenanthrene의 독성영향)

  • Choi, Hoon;Lee, Ju-Wook;Park, Yun-Ho;Lee, Seung-Min;Choi, Yoon-Seok;Heo, Seung;Hwang, Un-Ki
    • Korean Journal of Environmental Biology
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    • v.38 no.3
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    • pp.333-342
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    • 2020
  • The aim of this study was to define the toxic effects of phenanthrene (PAHs) on the fertilization and normal embryogenesis rates in the two species of sea urchin (Hemicentrotus pulcherrimus and Mesocentrotus nudus). The sperm and fertilized eggs of both sea urchin species were exposed to serial dilutions of phenanthrene for 10 min and 48 hours, respectively. The fertilization rate and normal embryogenesis rate of H. pulcherrimus and M. nudus were decreased in a concentration-dependent manner. The EC50 for the fertilization rate of H. pulcherrimus and M. nudus was 17.48 mg L-1 and 16.21 mg L-1, and the EC50 for the normal embryogenesis rate was 2.99 mg L-1 and 0.36 mg L-1, respectively. Between the two species, H. pulcherrimus was more sensitive to phenanthrene exposure, and 48 h normal embryogenesis was the more sensitive endpoint. Therefore, the results of this study demonstrated that the exposure of both sea urchin species to phenanthrene caused alterations in egg fertilization and the early developmental stages.

Carbon Mineralization in different Soils Cooperated with Barley Straw and Livestock Manure Compost Biochars (토양 종류별 보릿짚 및 가축분 바이오차 투입이 토양 탄소 무기화에 미치는 영향)

  • Park, Do-Gyun;Lee, Jong-Mun;Choi, Eun-Jung;Gwon, Hyo-Suk;Lee, Hyoung-Seok;Park, Hye-Ran;Oh, Taek-Keun;Lee, Sun-Il
    • Journal of the Korea Organic Resources Recycling Association
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    • v.30 no.4
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    • pp.67-83
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    • 2022
  • Biochar is a carbon material produced through the pyrolysis of agricultural biomass with limited oxygen condition. It has been suggested to enhance the carbon sequestration and mineralization of soil carbon. Objective of this study was to investigate soil potential carbon mineralization and carbon dioxide(CO2) emissions in different soils cooperated with barely straw and livestock manure biochars in the closed chamber. The incubation was conducted during 49 days using a closed chamber. The treatments consisted of 2 different biochars that were originated from barley straw and livestock manure, and application amounts were 0, 5, 10 and 20 ton ha-1 with different soils as upland, protected cultivation, converted and reclaimed. The results indicated that the TC increased significantly in all soils after biochar application. Mineralization of soil carbon was well fitted for Kinetic first-order exponential rate model equation (P<0.001). Potential mineralization rate ranged from 8.7 to 15.5% and 8.2 to 16.5% in the barely straw biochar and livestock manure biochar treatments, respectively. The highest CO2 emission was 81.94 mg kg-1 in the upland soil, and it was more emitted CO2 for barely straw biochar application than its livestock biochar regardless of their application rates. Soil amendment of biochar is suitable for barely straw biochar regardless of application rates for mitigation of CO2 emission in the cropland.

Sediment Toxicity Assessment in the Intertidal Flat Zone of the Middle West Coast of Korea

  • Hwang, G.S.;Dave, G.;Nilsson, E.;Kim, K.
    • Journal of Environmental Health Sciences
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    • v.30 no.4
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    • pp.347-351
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    • 2004
  • A battery of sediment bioassays was performed for the sediments from the intertidal flat zone along the middle west coast of Korea to assess their potential toxicity. In the bioassays, three crustaceans, Daphnia magna, Nitocra spines, and Hyalella aztec a were exposed to $16\%$ sediments (wet weight) collected from 14 sites. Immobility($\%$) was checked as an endpoint after 24- and 48-h exposure of Daphnia magna and after 96-h exposure of Hyalella azteca and Nitocra spines. Among the three bioassays, the 48-h Daphnia bioassay showed the most distinct differential sensitivity in relation to sediment contamination, while the Nitocra and the Hyalella bioassays failed to show the differential sensitivity properly among the sites classified as polluted. Significantly different levels of immobility ($\%$) were obtained between the sites classified as chemical/nutrient polluted and the sites classified as non-polluted in the Daphnia bioassays, but not in the Nitocra bioassay and the Hyalella bioassay. Some differences of toxic response to the same sediments among bioassays were observed, suggesting that there may be a chemical specificity of response sensitivity to sediment toxicity, due to differences in bio-availability of sediment toxicants among test species.

Application Case of Test of Revegetation Measures on Design of Slopes Revegetation and Tentative Instruction on Construction Work -With a Case of Slopes Along the National Road Between Nongseo and Eomo - (비탈면 녹화 설계 및 시공 잠정 지침 적용사례 -농소어모구간 국도비탈면을 중심으로-)

  • Jeon, Gi-Seong
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.10 no.4
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    • pp.95-108
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    • 2007
  • Test application of revegetation measure was made on the roadside slope damaged by Nongseo-Eomo national road improvement project in a bid to prevent the soil from being washed out as well as to restore the ecological environment, and the survey for assessment of the effect of slope revegetation measures was conducted, beginning May 11 through Nov 7, 2006. In the wake of comprehensive reviewing and evaluating the surrounding topographic environment, physical and chemical characteristics of soil, germination of revegetation plants, analysis of bio mass, covering ratio and the plants appeared, measure b was found to have been most appropriate to cut blasting rock slope, and alternatively measure c. For cut ripping rock slope, measure c-1 appeared to be effective in revegetation effect, and alternatively, b-1.When it comes to cut soil slope, measure c-2 was found to be effective, and b-2 to be a good alternative. And for embankment soil slope, measure b-3 appeared to be most efficient in revegetation effect and measure f as alternative.

Differential Behaviour of Selenium Analogs against Anticancer Drug Induced Apoptosis of Lymphocytes in Human Peripheral Blood

  • Elango, Sonaa;Subbiah, Usha;Jain, Jeong
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.5
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    • pp.2527-2533
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    • 2016
  • Sensitising cancer cells and at the same time desensitizing normal cells is a double task in cancer management. Agents which can combat the debilitating side effects of cancer therapeutics and simultaneously synergize with anticancer agents in specifically targeting cancer cells are needed. Selenium, a proven anticarcinogen, gains due importance in terms of its efficacy to combat the side effects of cancer therapy. This study is a comparative analysis of the chemoprotective effects of selenium compounds, methyl selenol (generated from organic selenomethionine (5mmol/L ; METase 40U/L)) and sodium selenite (inorganic form)($30{\mu}M$) in peripheral blood human lymphocytes exposed to cisplatin and mitomycin. Biochemical alterations occurring in many cells during apoptosis include loss of plasma membrane phospholipid asymmetry, DNA fragmentation, and activation of caspase-3. The present study demonstrated that the selenium metabolite and selenite are efficient in protecting lymphocytes undergoing DNA damage and exerted their activity by reducing caspase 3 expression. Interestingly organic methylselenol (MeSe) was found to offer more protective effects compared to inorganic selenite (SeL), by reducing the induction of apoptosis by the cytotoxic agents. This suggests that MeSe and to a lesser extent selenite might have potential for assessment in clinical trials and could be considered as strong candidates in pharmacogenomics or in the nutriprotective arena.

Numerical Method for Exposure Assessment of Wireless Power Transmission under Low-Frequency Band

  • Kim, Minhyuk;Park, SangWook;Jung, Hyun-Kyo
    • Journal of Magnetics
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    • v.21 no.3
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    • pp.442-449
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    • 2016
  • In this paper, an effective numerical analysis method is proposed for calculating dosimetry of the wireless power transfer system operating low-frequency ranges. The finite-difference time-domain (FDTD) method is widely used to analyze bio-electromagnetic field problems, which require high resolution, such as a heterogeneous whole-body voxel human model. However, applying the standard method in the low-frequency band incurs an inordinate number of time steps. We overcome this problem by proposing a modified finite-difference time-domain method which utilizes a quasi-static approximation with the surface equivalence theorem. The analysis results of the simple model by using proposed method are in good agreement with those from a commercial electromagnetic simulator. A simulation of the induced electric fields in a human head voxel model exposed to a wireless power transmission system provides a realistic example of an application of the proposed method. The simulation results of the realistic human model with the proposed method are verified by comparing it with the conventional FDTD method.

Technical and clinical aspects of cortisol as a biochemical marker of chronic stress

  • Lee, Do Yup;Kim, Eosu;Choi, Man Ho
    • BMB Reports
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    • v.48 no.4
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    • pp.209-216
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    • 2015
  • Stress is now recognized as a universal premorbid factor associated with many risk factors of various chronic diseases. Acute stress may induce an individual's adaptive response to environmental demands. However, chronic, excessive stress causes cumulative negative impacts on health outcomes through "allostatic load". Thus, monitoring the quantified levels of long-term stress mediators would provide a timely opportunity for prevention or earlier intervention of stressrelated chronic illnesses. Although either acute or chronic stress could be quantified through measurement of changes in physiological parameters such as heart rate, blood pressure, and levels of various metabolic hormones, it is still elusive to interpret whether the changes in circulating levels of stress mediators such as cortisol can reflect the acute, chronic, or diurnal variations. Both serum and salivary cortisol levels reveal acute changes at a single point in time, but the overall long-term systemic cortisol exposure is difficult to evaluate due to circadian variations and its protein-binding capacity. Scalp hair has a fairy predictable growth rate of approximately 1 cm/month, and the most 1 cm segment approximates the last month's cortisol production as the mean value. The analysis of cortisol in hair is a highly promising technique for the retrospective assessment of chronic stress. [BMB Reports 2015; 48(4): 209-216]