Browse > Article
http://dx.doi.org/10.14480/JM.2022.20.4.199

Detection of single-nucleotide polymorphism in RPB2 of Wolfiporia hoelen strains and assessment of its applicability for strain breeding  

Su Yeon, Kim (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science)
Mi-Jeong, Park (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science)
Seong Hwan, Kim (Department of Microbiology, College of Science & Technology, Dankook University)
Kang-Hyeon, Ka (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science)
Publication Information
Journal of Mushroom / v.20, no.4, 2022 , pp. 199-207 More about this Journal
Abstract
The demand for novel strains has been rising in the domestic market to increase the production of sclerotia from Wolfiporia hoelen. To improve strain breeding efficiency, we investigated whether single-nucleotide polymorphisms (SNPs) in the RNA polymerase II subunit (RPB2) gene, which may be linked to the mating type locus, are useful for distinguishing monokaryons from dikaryons in Korean W. hoelen strains. We designed a specific primer set to efficiently amplify a region of RPB2 using PCR with the genomic DNA of 12 cultivated strains and 31 wild strains of W. hoelen collected from Korea. Nucleotide sequences of the PCR-amplified RPB2 genes were determined and analyzed for the presence of SNPs among the 43 W. hoelen strains. Previously reported SNP loci were detected in the RPB2 gene of all W. hoelen strains tested. However, these previously reported SNP loci could not be applied to differentiate monokaryons from dikaryons in approximately one-third of Korean wild strains with homozygous genotypes. Three additional SNPs in the RPB2 gene, which may improve the ability to distinguish monokaryons from dikaryons, were identified by searching through the multiple sequence alignments of the 43 W. hoelen strains. The applicability of these three novel SNPs, together with the previously known SNPs, in the RPB2 gene to W. hoelen strain breeding was verified by examining the hybrid strains and their parental strains.
Keywords
Breeding; Mating type; RPB2; Single-nucleotide polymorphism; Wolfiporia hoelen;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Wang YZ, Zhang J, Zhao YL, Li T, Shen T, Li JQ, Li WY, Liu HG. 2013. Mycology, cultivation, traditional uses, phytochemistry and pharmacology of Wolfiporia cocos (Schwein.) Ryvarden et Gilb.: a review. J Ethnopharmacol 147: 265-276.   DOI
2 Zhao YY, Feng YL, Du X, Xi ZH, Cheng XL, Wei F. 2012. Diuretic activity of the ethanol and aqueous extracts of the surface layer of Poria cocos in rat. J Ethnopharmacol 114: 775-778. 
3 Au CH, Wong MC, Bao D, Zhang M, Song C, Song W, Law PTW, Kues U, Kwan HS. 2014. The genetic structure of the A mating-type locus of Lentinula edodes. Gene 535: 184-190.   DOI
4 Igari N, Minoura S, Okada M. 1995. Multinucleate nature, and mating by use of isozyme analysis in Poria cocos. Mycoscience 36: 405-411.   DOI
5 Jang YA, Lee JT. 2015. The evaluation of antioxidant, antiinflammatory, and anti-aging of extract solvent and Poria cocos by parts. Kor J Aesthet Cosmetol 13: 377-383.
6 Jo WS, Lee SH, Koo J, Ryu S, Kang MG, Lim SY, Park SC. 2017. Morphological characteristics of fruit bodies and basidiospores of Wolfiporia extensa. J Mushrooms 15: 54-56.   DOI
7 Jo WS, Yoo YB, Hong IP, Kim D. 2013. Changes of the cultivation methods of Poria cocos and its commercialization. J Mushrooms 11: 303-307.   DOI
8 Ka KH, Kim S, Park MJ, Jeong YS, Ryoo R, Jang Y, Choi JW, Kim SH. 2021. Development of new strains of Wolfiporia cocos for sclerotium formation by 2-way crossbreeding. Kor J Mycol 49: 405-412.   DOI
9 Kim YS. 1998. Effects of Poria cocos powder on wet noodle qualities. Appl Biol Chem 41: 539-544.
10 Li S, Dong C. 2022. A new type of homokaryotic strain of Wolfiporia hoelen with indistinguishable phenotypes from the parent strains. Mycosystema 41: 1279-1292.
11 Li S, Wang Q, Dong C. 2021. Distinguishing homokaryons and heterokaryons in medicinal polypore mushroom Wolfiporia cocos (Agaricomycetes) based on cultural and genetic characteristics. Front Microbiol 11: 596715.   DOI
12 Li S, Wang Q, Dong C. 2022. Bipolar system of sexual incompatibility and heterothallic life cycle in the basidiomycetes Pachyma hoelen Fr. (Fuling). Mycologia 114: 63-75.   DOI
13 Niculita-Hirzel H, Labbe J, Kohler A, Le Tacon F, Martin F, Sanders IR, Kues U. 2008. Gene organization of the mating type regions in the ectomycorrhizal fungus Laccaria bicolor reveals distinct evolution between the two mating type loci. New Phytol 180: 329-342.   DOI
14 Rocha de Brito M, Foulongne-Oriol M, Moinard M, Souza Dias E, Savoie JM, Callac P. 2016. Spore behaviors reveal a category of mating-competent infertile heterokaryons in the offspring of the medicinal fungus Agaricus subrufescens. Appl Microbiol Biotechnol 100: 781-796.   DOI