• Journal of Life Science
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• v.27 no.9
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• pp.1070-1077
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• 2017

Chondrocyte apoptosis induced by reactive oxygen species (ROS) plays an important role in the pathogenesis of osteoarthritis. Schisandrin A, a bioactive compound found in fruits of the Schisandra genus, has been reported to possess multiple pharmacological and therapeutic properties. Although several studies have described the antioxidant effects of analogues of schisandrin A, the underlying molecular mechanisms of this bioactive compound remain largely unresolved. The present study investigated the cytoprotective effect of schisandrin A against oxidative stress (hydrogen peroxide [$H_2O_2$]) in SW1353 human chondrocyte cells. The results showed that schisandrin A preconditioning significantly inhibited $H_2O_2-induced$ growth inhibition and apoptotic cell death by blocking the degradation of poly (ADP-ribose) polymerase proteins and down-regulating pro-caspase-3. These antiapoptotic effects of schisandrin A were associated with attenuation of mitochondrial dysfunction and normalization of expression changes of proapoptotic Bax and antiapoptotic Bcl-2 in $H_2O_2-stimulated$ SW1353 chondrocytes. Furthermore, schisandrin A effectively abrogated $H_2O_2-induced$ intracellular ROS accumulation and phosphorylation of histone H2AX at serine 139, a widely used marker of DNA damage. Thus, the present study demonstrates that schisandrin A provides protection against $H_2O_2-induced$ apoptosis and DNA damage in SW1353 chondrocytes, possibly by prevention of ROS generation. Collectively, our data indicate that schisandrin A has therapeutic potential in the treatment of oxidative disorders caused by overproduction of ROS.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.4
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• pp.394-400
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• 2008

Crop characteristics of 137 common millet (Panicum miliaceum L.) resources collected in Korea were investigated in order to establish basic data for the improvement of crop breeding. Spike types of 137 plant resources were classified as 4 types of oblate, spreading, oblate-compact (intermediary type between oblate and compact) and oblate-spreading (intermediary type between oblate and compact) type, of which oblate spike type was the highest percentage of 66.4% (91 plant resources) of 137 germplasm. Seed color was classified as 5 colors of dark brown, yellow, brown, yellowish brown, and yellowish white, of which yellowish white was the highest percentage of 66.4% (54 plant resources) among them. Seed type was classified as 2 types of pyramid shape and short pyramid shape, which showed 62.8% and 37.2% respectively. Days from seeding to heading date showed the range from 68 to 85 days with 73.7 days in the average and the highest frequency proportion of it was the group from 70 to 75 days, which occupied 46.7% (64 plant resources) of 137 germplasm. Days from seeding to physiological maturity showed the range from 109 to 124 days with 112.0 days in the average and the highest frequency proportion (60.6%) of it was the group from 111 to 115 days, while there were also occupied with 6.6% below 110 days and 5.1% over 120 days among 137 germplasm. Group from 181 cm to 190 cm of culm length showed the highest frequency proportion of 47.4%, whereas below 160 cm was the lowest occupancy of 2.9%. Spike length showed the range from 181 to 190 cm with 181.4 cm in the average and the highest frequency distribution of it was the group from 31 to 35 cm with the proportion of 54.0%. Number of grains per spike showed the range from 432 to 2,665 grains with 1,429.5 grains in the average and group from 1,200 to 1,400 grains per spike was the highest frequency distribution with the proportion of 20.4%. There were also occupied with 13.1% below 1,000 grains and 8.6% over 2,000 grains per spike among 137 germplasm.

• Journal of Life Science
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• v.23 no.11
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• pp.1397-1403
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• 2013

Fructus Sophorae, the dried ripe fruit of Styphnolobium japonicum (L.), is an herbal ingredient used in traditional Oriental medicine. This study was carried out to investigate the effects of Fructus Sophorae extracts (FSE) on immune modulation in a murine RAW 264.7 macrophage model. As immune response parameters, the production of prostaglandin $E_2$ ($PGE_2$) and tumor necrotic $factor-{\alpha}$ ($TNF-{\alpha}$) were evaluated. Our data revealed that FSE increased the macrophage activation and the production of $PGE_2$ and $TNF-{\alpha}$, which was consistently correlated with upregulation of cyclooxygenase-2 (COX-2) and $TNF-{\alpha}$ expression at both transcriptional and translational levels. On comparative cytokine protein array, FSE significantly increased several cytokines, which was associated with phosphorylation of mitogen- activated protein kinases (MAPKs), including extracellular signal-regulated kinase (ERK), p38 MAPK and c-Jun N-terminal kinase (JNK), and Akt in RAW 264.7 cells. However, each inhibitor of these molecules attenuated the FSE-induced $PGE_2$ production. These results indicate that FSE activated macrophages through the activation of MAPKs and phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathways in RAW 264.7 macrophages. These findings suggest that FSE may provide a promising source of an immunoenhancing agent.

• Journal of agriculture & life science
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• v.43 no.1
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• pp.51-59
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• 2009

The chemical components and antimicrobial activities of garlic from different area were investigated and analyzed to provide basic data for functional food materialization and processing. Hunter's values of garlic from different area were L 53.41~57.15, a -3.49~-4.38 and b 11.47~17.55. The moisture, crude protein, crude fat, nitrogen free extract, crude fiber and ash were 65.24~71.96, 6.24~9.35, 0.21~0.49, 19.01~22.72, 0.58~0.95 and 1.01~2.01%, respectively. The major minerals of garlic from different area were Na(27.22~112.03), Mg(18.17~32.56), K(242.16~569.28), Ca(28.60~63.93), P(117.72~265.21 mg%) and major free sugars were sucrose, glucose and fructose. The major amino acids of garlic from different area were proline, arglmne, glutamic acid and aspartic acid and content of total amino acid was 2,709.33~4,561.04 mg%. The ascorbic acid content of garlic from different area was 2.966~8.673 mg%. Composition of fatty acids of garlic from different area were linoleic acid, oleic acid and palmitic acid, unsaturated fatty acid and saturated fatty acid contents were 72.18~74.35 and 25.65~27.82%, respectively. Antimicrobial activities of garlic extracts as different area increased depends on concentration and showed the high antimicrobial activities against Gram(+) and Gram(-).

• Journal of Bio-Environment Control
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• v.28 no.4
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• pp.376-387
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• 2019

Melons are mostly grown in soil, but it is susceptible to damage due to injury by continuous cropping such as Fusarium wilt and root rot. Hydroponic cultivation system can overcome the disadvantages of soil cultivation with precise nutrition management and a clean environment. When using the coir substrate, the most environmentally friendly organic substrate used for hydroponics, it is analyzed how the growth and fruit quality of the melon depends on the ratio of chips and dust and the amount of irrigation. The purpose of this study was to provide the basic data of melon hydroponics when cultivated in spring. The two types of the coir substrates used in the experiments were chip and dust ratios of 3 :7 and 5 : 5 respectively. The substrate with high dust ratios had excellent physical characteristics, such as container capacity and total porosity, and the drainage EC level showed a high value of $3.0-6.8dS{\cdot}m^{-1}$. When the amount of irrigation is provided based on the drainage rate, the group provided the nutrient solution on the basis of 10% drainage supplied 91 L per plant, which was reduced by about 30% compared to the group with the highest water supply. In addition, the total drainage showed less than 10 L per plant with a minimum water supply and was reduced by 30 - 70% in substrate with a high dust rates. In substrate with high water supply and high dust ratio, leaf growth and fruit enlargement were good, and the soluble solids content varies greatly from cultivar to cultivar. If you provided the amount of irrigation based on 10% drainage rate, the fruit weight will be decreased, but the amount of irrigation can be reduced. Therefore, it is considered that managing the water & nutrient properly taking into account the characteristics of coir substrate and cultivar can produce melon of uniform quality using hydroponics.

• Journal of Bio-Environment Control
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• v.28 no.1
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• pp.1-8
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• 2019

The study aimed to determine effects of light emitting diode (LED) and the ultraviolet radiation (UVA) light of plant factory on plant growth and ascorbic acid content of spinach (Spinacia oleracea cv. Shusiro). Plants were grown in a NFT (Nutrient Film Technique) system for 28 days after transplanting with fluorescent light (FL, control), LEDs and UVA (Blue+UVA (BUV), Red and Blue (R:B(2:1)) + UVA (RBUV), Red+UVA (RUV), White LED (W), Red and Blue (R:B(2:1)), Blue (B), Red (R)) under the same light intensity ($130{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and photoperiod (16/8h = day/night). All the light sources containing the R (R, RB, RUV, and RBUV) showed leaf epinasty symptom at 21 days after transplanting (DAT). Under the RUV treatment, the lengths of leaf and leaf petiole were significantly reduced and the leaf width was increased, lowering the leaf shape index, compared to the R treatment. Under the BUV, however, the lengths of leaf and leaf petiole were increased significantly, and the leaf number was increased compared to B. Under the RBUV treatment, the leaf length was significantly shorter than other treatments, while no significant difference between the RBUV and RB for the fresh and dry weights and leaf area. Dry weights at 28 days after transplanting were significantly higher in the R, RUV and BUV treatments than those in the W and FL. The leaf area was significantly higher under the BUV treatment. The ascorbic acid content of the 28 day-old spinach under the B was significantly higher, followed by the BUV, and significantly lower in FL and R. All the integrated data suggest that the BUV light seems to be the most suitable for growth and quality of hydroponically grown spinach in a plant factory.

• Journal of Bio-Environment Control
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• v.28 no.4
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• pp.335-341
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• 2019

In order to provide basic data for uniformization of temperature distribution in heating greenhouses, heating experiments were performed in two greenhouses with a hot water heating system. By analyzing heat transfer characteristics and improving pipes layout, measures to reduce the variation of pipe surface temperature and to improve the uniformity were derived. As a result of analyzing the temperature distributions of two different greenhouses and examining the maximum deviation and uniformity, it was found that the temperature deviation of greenhouses with a large amount of hot water flow and a short heating pipe was small and the uniformity was high. And it was confirmed that the temperature deviation was reduced and the uniformity was improved when the circulating fan was operated. The correlation between the surface temperature of the heating pipe and the indoor air temperature was a positive correlation and statistically significant(p<0.01) in both greenhouses. It was confirmed that the indoor temperature distribution in a hot water heating greenhouse was influenced by the surface temperature distribution of heating pipe, and the uniformity of indoor temperature distribution could be improved by arranging the heating pipe to minimize the temperature deviation. Analysis of the heat transfer characteristics of heating pipe showed that the temperature deviation increased as the pipe length became longer and the temperature deviation became smaller as the flow rate in pipe increased. Therefore, it was considered that the temperature distribution and the uniformity of environment in a greenhouse could be improved by arranging the heating pipe to shorten the length and controlling the flow velocity in pipe. In order to control the temperature deviation of one branch pipe within $3^{\circ}C$ in the tube rail type hot water heating system most used in domestic greenhouses, when the flow velocity in the pipe is 0.2, 0.4, 0.6, 0.8, $1.0m{\cdot}s^{-1}$, the length of a heating pipe should be limited to 40, 80, 120, 160, 200m, respectively.

• Journal of Bio-Environment Control
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• v.28 no.4
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• pp.366-375
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• 2019

This experiment aims to determine the proper irrigation scheduling based on a whole-substrate capacitance using a newly developed device (SCMD) by comparing with the integrated solar radiation automated irrigation system (ISR) and sap flow sensor automated irrigation system (SF) for the cultivation of tomato (Solanum lycopersicum L. 'Hoyong' 'Super Doterang') during spring to winter season. For the SCMD system, irrigation was conducted every 10 minutes after the first irrigation was started until the first run-off was occurred, of which the substrate capacitance was considered to be 100%. When the capacitance threshold (CT) was reached to the target point, irrigation was re-conducted. After that, when the target drain volume (TDV) was occurred, the irrigation stopped. The irrigation volume per event for the SCMD was set to 50, 75, or 100 mL at CT 0.9 and TDV 100 mL during the spring to summer cultivation, and the CT was set to 0.65, 0.75, 0.80, or 0.90 in the winter cultivation. When the irrigation volume per event was set to 50, 75, or 100 mL, the irrigation frequency in a day was 39, 29, and 19, respectively, and the drain rate was 3.04, 9.25, and 20.18%, respectively. When the CT was set to 0.65, 0.75, or 0.90 in winter, the irrigation frequency was about 6, 7, 15 times, respectively and the drain rate was 9.9, 10.8, 35.3% respectively. The signal of stem sap flow at the beginning of irrigation starting time did not correspond to that of solar irradiance when the irrigation volume per event was set to 50 or 75 mL, compared to that of 100 mL. In winter cultivation, the stem sap flow rate and substrate volumetric water content at the CT 0.65 treatment were very low, while they were very high at CT 0.90 was high. All the integrated data suggest that the proper range of irrigation volume per event is from 75 to 100 mL under at CT 0.9 and TDV 100 mL during the spring to summer cultivation, and the proper CT seems to be higher than 0.75 and lower than 0.90 under at 75 mL of the irrigation volume per event and TDV 70 mL during the winter cultivation. It is going to be necessary to investigate the relationship between capacitance value and substrate volumetric water content by determining the correction coefficient.

• Journal of the Korean Wood Science and Technology
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• v.49 no.4
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• pp.336-359
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• 2021

In this study, hybrid CLT research and development trends were analyzed to improve the low rolling shear strength of CLT, a large wooden panel used in high-rise wooden buildings. Through this, basic data that can be used in research and development directions for localization of CLT were prepared. As a way to improve the low rolling shear strength, the use of hardwood lamina, the change of the lamina arrangement angle, and the use of structural composite materials are mainly used. Rolling shear strength and shear modulus of hardwood lamina are more than twice as high as softwood lamina. It confirmed that hardwoods can be used and unused species can be used. Rolling shear strength 1.5 times, shear modulus 8.3 times, bending stiffness 4.1 times improved according to the change of the layer arrangement angle, and the CLT strength was confirmed by reducing the layer arrangement angle. Structural wood-based materials have been improved by up to 1.35 times MOR, 1.5 times MOE, and 1.59 times rolling shear strength when used as laminas. Block shear strength between the layer materials was also secured by 7.0 N/mm², which is the standard for block shear strength. Through the results of previous studies, it was confirmed that the strength performance was improved when a structural wood based materials having a flexural performance of MOE 7.0 GPa and MOR 40.0 MPa or more was used. This was determined based on the strength of layered materials in structural wood-based materials. The optimal method for improving rolling shear strength is judged to be the most advantageous application of structural wood based materials with strength values according to existing specifications. However, additional research is needed on the orientation of CLT lamina arrangement according to the fiber arrangement of structural wood-based materials, and the block shear strength between lamina materials.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.409-415
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• 2022

Plant growth and development are also affected by root-zone environment. Therefore, it is important to consider the variables of the root-zone environment when establishing an irrigation strategy. The purpose of this study is to analyze the relationship between the volumetric moisture content (VWC), Bulk EC (ECb), and Pore EC (ECp) used by plant roots using FDR sensors in two types of rockwool media with different water transmission characteristics, using the method above this was used to establish a method for collecting and correcting available root-zone environmental data. For the experiment, two types of rockwool medium (RW1, RW2) with different physical characteristics were used. The moisture content (MC) and ECb were measured using an FDR sensor, ECp was measured after extracting the residual nutrient solution from the medium using a disposable syringe in the center of the medium at a volumetric moisture content (VWC) of 10-100%. Then, ECb and ECp are measured by supplying nutrient solution having different concentration (distilled water, 0.5-5.0) to two types of media (RW1, RW2) in each volume water content range (0 to 100%). The relationship between ECb and ECp in RW1 and RW2 media is best suited for cubic polynomial. The relationship between ECb and ECp according to volume moisture content (VWC) range showed a large error rate in the low volume moisture content (VWC) range of 10-60%. The correlation between the sensor measured value (ECb) and the ECp used by plant roots according to the volumetric water content (VWC) range was the most suitable for the Paraboloid equation in both media (RW1, RW2). The coefficient of determination the calibration equation for RW1 and RW2 media were 0.936, 0.947, respectively.