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http://dx.doi.org/10.5352/JLS.2017.27.9.1070

Cytoprotective Effects of Schisandrin A against Hydrogen Peroxide-induced Oxidative Stress in SW1353 Human Chondrocytes  

Jeong, Jin-Woo (Open Lab. for Muscular & Skeletal Disease Control and Department of Biochemistry and Dongeui University College of Korean Medicine)
Choi, Eun Ok (Open Lab. for Muscular & Skeletal Disease Control and Department of Biochemistry and Dongeui University College of Korean Medicine)
Kwon, Da Hye (Open Lab. for Muscular & Skeletal Disease Control and Department of Biochemistry and Dongeui University College of Korean Medicine)
Kim, Bum Hoi (Department of Anatomy, Dongeui University College of Korean Medicine)
Park, Dong Il (Department of Internal Medicine, Dongeui University College of Korean Medicine)
Hwang, Hye Jin (Anti-Aging Research Center and Blue Bio Industry RIC, Dongeui University)
Kim, Byung Woo (Anti-Aging Research Center and Blue Bio Industry RIC, Dongeui University)
Choi, Yung Hyun (Open Lab. for Muscular & Skeletal Disease Control and Department of Biochemistry and Dongeui University College of Korean Medicine)
Publication Information
Journal of Life Science / v.27, no.9, 2017 , pp. 1070-1077 More about this Journal
Abstract
Chondrocyte apoptosis induced by reactive oxygen species (ROS) plays an important role in the pathogenesis of osteoarthritis. Schisandrin A, a bioactive compound found in fruits of the Schisandra genus, has been reported to possess multiple pharmacological and therapeutic properties. Although several studies have described the antioxidant effects of analogues of schisandrin A, the underlying molecular mechanisms of this bioactive compound remain largely unresolved. The present study investigated the cytoprotective effect of schisandrin A against oxidative stress (hydrogen peroxide [$H_2O_2$]) in SW1353 human chondrocyte cells. The results showed that schisandrin A preconditioning significantly inhibited $H_2O_2-induced$ growth inhibition and apoptotic cell death by blocking the degradation of poly (ADP-ribose) polymerase proteins and down-regulating pro-caspase-3. These antiapoptotic effects of schisandrin A were associated with attenuation of mitochondrial dysfunction and normalization of expression changes of proapoptotic Bax and antiapoptotic Bcl-2 in $H_2O_2-stimulated$ SW1353 chondrocytes. Furthermore, schisandrin A effectively abrogated $H_2O_2-induced$ intracellular ROS accumulation and phosphorylation of histone H2AX at serine 139, a widely used marker of DNA damage. Thus, the present study demonstrates that schisandrin A provides protection against $H_2O_2-induced$ apoptosis and DNA damage in SW1353 chondrocytes, possibly by prevention of ROS generation. Collectively, our data indicate that schisandrin A has therapeutic potential in the treatment of oxidative disorders caused by overproduction of ROS.
Keywords
Antioxidant; apoptosis; DNA damage; ROS; schisandrin A;
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