Chemical Components and Antimicrobial Activity of Garlics from Different Cultivated Area

산지별 마늘의 화학성분 및 항균활성

  • 정창호 (진주산업대학교 식품과학과) ;
  • 배영일 (Bio 21 센터) ;
  • 이진화 (진주산업대학교 인테리어소재공학과) ;
  • 노정관 (진주산업대학교 인테리어소재공학과) ;
  • 신창식 (동원F&B 식품과학연구원) ;
  • 최진상 (진주산업대학교 식품과학과) ;
  • 심기환 (경상대학교 응용생명과학부.농업생명과학연구원)
  • Received : 2008.06.10
  • Accepted : 2009.02.20
  • Published : 2009.02.28

Abstract

The chemical components and antimicrobial activities of garlic from different area were investigated and analyzed to provide basic data for functional food materialization and processing. Hunter's values of garlic from different area were L 53.41~57.15, a -3.49~-4.38 and b 11.47~17.55. The moisture, crude protein, crude fat, nitrogen free extract, crude fiber and ash were 65.24~71.96, 6.24~9.35, 0.21~0.49, 19.01~22.72, 0.58~0.95 and 1.01~2.01%, respectively. The major minerals of garlic from different area were Na(27.22~112.03), Mg(18.17~32.56), K(242.16~569.28), Ca(28.60~63.93), P(117.72~265.21 mg%) and major free sugars were sucrose, glucose and fructose. The major amino acids of garlic from different area were proline, arglmne, glutamic acid and aspartic acid and content of total amino acid was 2,709.33~4,561.04 mg%. The ascorbic acid content of garlic from different area was 2.966~8.673 mg%. Composition of fatty acids of garlic from different area were linoleic acid, oleic acid and palmitic acid, unsaturated fatty acid and saturated fatty acid contents were 72.18~74.35 and 25.65~27.82%, respectively. Antimicrobial activities of garlic extracts as different area increased depends on concentration and showed the high antimicrobial activities against Gram(+) and Gram(-).

마늘을 각종 기능성 식품 재료로 이용하기 위한 기초자료를 제공하기 위하여 산지별 마늘의 화학성분 및 항균활성을 조사하였다. 색도 중 L값은 53.41~57.15, a값은 -3.49~-4.38 및 b값은 11.47~17.55였으며, 일반성분 중 수분, 조단백질, 조지방, 가용성 무질소물, 조섬유 및 회분 함량은 각각 65.24~71.96, 6.24~9.35, 0.21~0.49, 19.01~22.72, 0.58~0.95 및 1.01~2.01%로 나타났다. 산지별 마늘의 주요 무기성분으로는 Na, Mg, K, Ca 및 P였으며, 그 함량은 각각 27.22~112.03, 18.17~32.56, 242.16~569.28, 28.60~63.93 및 117.72~265.21 mg%였고, 유리당은 sucrose, glucose 및 fructose였다. 마늘은 17종의 아미노산이 분석되었으며, 그 중 proline, arginine, glutamic acid 및 aspartic acid가 주요 아미노산으로 나타났고, 총 아미노산 함량은 2,709.33~4,561.04 mg%이었다. 비타민 C 함량은 2.966~8.673 mg%이었다. 산지별 마늘의 주요 지방산으로는 linoleic acid, oleic acid 및 palmitic acid였으며, 불포화지방산이 72.18~74.35%였고, 포화지방산은 25.65~27.82%였다. 산지별 마늘물 추출물을 이용하여 항균활성을 측정한 결과, 농도의존적으로 그램 음성 및 양성 모든 세균에서 높은 항균활성을 보였다.

Keywords

Acknowledgement

Supported by : 산업자원부

References

  1. Al-Delaimy, K. S. and M. M. Barakat. 1970. Antimicrobial and preservative activity of garlic on fresh ground camel meat. I. Effect of fresh ground garlic segments. J. Sci. Food Agric. 21: 110-112. https://doi.org/10.1002/jsfa.2740210214
  2. AOAC. 1990. Offical methods of analysis. Washington DC, Association of Offical Analytical Chemists.
  3. Cavallito, C. J. and H. Bailey. 1994. Alliin, the antibacterial principle of Allium sativum. I. Isolation, physical properties, and antibacterial action. J. Am. Chem. Soc. 66: 1950-1956. https://doi.org/10.1021/ja01239a048
  4. Cavallito, C. J., J. S. Buck, and C. M. Suter. 1944. Alliin the antibacterial principle of Allium sativum. II. Determination of the chemical structure. J. Am. Chem. Soc. 66: 1952-1954. https://doi.org/10.1021/ja01239a049
  5. Choi, J. H., J. G. Jang, K. D. Park, and S. K. Oh. 1981. High performance liquid chromatographic determination of free sugars in ginseng and its products. Korean J. Food Sci. Technol. 13: 107-113.
  6. Dewit, J. C., S. Notermans, N. Gorin, and E. H. Kampelmacher. 1979. Effect of garlic oil or toxin production by Clostridium botulium in meat slurry. J. Food Protect. 42: 222-227.
  7. Farag, R. S. 1989. Antimicrobial activity of some Egyptian spice essential oils. J. Food Prot. 52: 665-670.
  8. Haciseferogllari, H., M. Ozcan, F. Demir, and S. Calısır. 2005. Some nutritional and technological properties of garlic(Allium sativum L.). J. Food Engineer. 68: 463-469. https://doi.org/10.1016/j.jfoodeng.2004.06.024
  9. Hwang, J. B., J. H. Ha, W. S. Park, and Y. C. Lee. 2004. Changes of component on green discolored garlic. Korean J. Food Sci. Technol. 36: 1-8.
  10. Jeong, C. H., Y. I. Bae, and K. H. Shim. 2000. Physicochemical properties of Hovenia dulcis Thunb. leaf tea. Korean J. Postharvest Sci. Technol. 7: 117-123.
  11. Jo, J. S. 1990. Food materials, Gijeunyungusa, Seoul, p. 154-155.
  12. Kamanna, V. S. and N. Chandrasekhara. 1983. Biochemical and physiological effects of garlic(Allium sativum Linn.). J. Sci. and Industrial Res. 42: 353-359.
  13. Kim, C. M., M. K. Shin, D. K. Ahn, and K. J. Lee. 1997. Wanyeok Jungyakdaisajeon, Jeongdam. pp 947-956. Seoul, Korea.
  14. Kim, E. S. and H. J. Chun. 1993. The Anticarcinogenic effect of garlic juice against DMBA induced carcinoma on the hamster buccal pouch. J. Kor. Soc. Food Nutr. 22: 398-404.
  15. Kwon, O. C., K. S. Woo, T. M. Kim, D. J. Kim, J. T. Hong, and H. S. Jeong. 2006. Physicochemical characteristics of garlic(Allium sativum L.) on the high temperature and pressure treatment. Korean J. Food Sci. Technol. 38: 331-336.
  16. Kwon, S. K. 2003. Organosulfur compounds from Allium sativum and physiological activities. J. Appl. Pharmacol. 11: 8-32.
  17. Lee, J. M., T. Y. Cha, S. H. Kim, T. K. Kwon, J. H. Kwon, and S. H. Lee. 2004. Monitoring on extraction conditions for physicochemical qualities of ethanol extract from garlic. J. Korean Soc. Food Sci. Nutr. 36 : 1198-1204. https://doi.org/10.3746/jkfn.2007.36.9.1198
  18. Lee, J. W., H. O. Lee, S. K. HO, S. K. Lee, J. H. Do, and M. W. Kim. 1997. Comparison of the chemical components between fresh and odorless garlic. Agric. Chem. Biotechnol. 40: 400-403.
  19. Metcalf, L. D., A. A. Schmits, and J. R. Pelka. 1966. Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Anal. Chem. 38: 514-515. https://doi.org/10.1021/ac60235a044
  20. Nishimura, H, W. Hanny, and J. Mizutani. 1988. Volatile flavor components and antithrombotic agent: Vinyldithinins from Allium victorialis. J. Agric. Food Chem. 36: 563-568. https://doi.org/10.1021/jf00081a039
  21. Ruffin, J and S. A. Hunter. 1983. An evaluation of the effect of garlic as an antihypertensive agent. Ctyobios. 37: 85-89.
  22. Shin, J. H., J. C. Ju, O. C. Kwen, S. M. Yang, S. J. Lee, and N. J. Sung. 2004. Physicochemical and physiological activities of garlic from different area. Korean J. Food & Nutr. 17: 237-245
  23. Small, L. D., J. H. Bailey, and C. J. Cavallito. 1947. Alkyl thiosulfinates. J. Am. Chem. Soc. 69: 1710-1716. https://doi.org/10.1021/ja01199a040
  24. Small, L. D., J. H. Bailey, and C. J. Cavallito. 1949. Comparison of some properties of thiosulfonates and thiosulfinates. J. Am. Chem. Soc. 71: 3565-3571. https://doi.org/10.1021/ja01178a531
  25. Yang, K. Y. and H. S. Shin. 1982. Lipids and fatty acid composition of garlic(Allium sativum Linnaeus). Korean J. Food Sci. Technol. 14: 388-393.
  26. Yu, T. H., C. M. Wu, and Y. C. Liou. 1989. Volatile compounds from garlic. J. Agric. Food Chem. 37: 725-730. https://doi.org/10.1021/jf00087a032