• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.116-116
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• 2002

Further development of reconstructed embryos may be dependent upon the synchronization of donor nucleus and recipient cytoplasm at cell fusion, To control the synchronization of donor and recipient cells, the enucleated MII arrested oocytes are artificially stimulated prior to embryo reconstruction. Destruction of cyclin B results in the exit of cells from M-phase of cell cycle. This study was designed to investigate the effects of single or combined stimulation affected cyclin B1 mRNA and protein levels in mouse oocytes. The oocyte activation was induced by 7% ethanol or 10$\mu\textrm{g}$/$m\ell$ Ca-ionophore without (single) or with (combined) 10$\mu\textrm{g}$/$m\ell$ cycloheximide. Competitive quantitative PCR for cyclin Bl mRNA and western blot analysis for cyclin B1 protein was preformed in mouse oocytes. Cyclin B1 mRNA level was significantly reduced in single (P<0.05) and combined (P<0.05) stimulation groups. However, this level did not change in non-activated group and increased in intact group. Cyclin B1 protein level was also significantly reduced in both single (P<0.05) and combined (P<0.05) stimulation groups. In conclusion, single and combined stimulation induces the degradation of cyclin B1 mRNA and protein after activation in enucleated mouse oocytes.

• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.381-385
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• 2000

The effect and interaction of Ca and B on exopolysaccharide (EPS) synthesis in the diazotrophically growing cyanobacterium. Nostoc spongiaeforme, was investigated. The absence of B inhibited EPS synthesis 1.56-fold ($16\mu\textrm{g}$ glucose equivalent/mg dry weight, 16 d) over the control cells ($25\mu\textrm{g}$ glucose equivalent) grown in medium containing 0.5 mM Ca and $8{\mu}{\textrm}{m}$ B. When B concentration was raised to $40{\mu}{\textrm}{m}$, EPS production was stimulated 1.8-fold. Reduction of Ca concentraion to one-half (0.25 mM) resulted in increased B demand (16$\muM$) by the cells for EPS production at par with the normal sets. However, without Ca, EPS production also increased as B increased. Addition of B to a Ca-free medium stimulated cyanobacterial diazotrophic growth as well as synthesis of Chl a and phycocyanin (0-8 d). The data suggest B-dependent diazotrophic growth during Ca-deficiency and point to and important interactive role of Ca and B in regulation of cyanobacterial EPS synthesis.

• Journal of Korean Institute of Industrial Engineers
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• v.28 no.1
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• pp.36-43
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• 2002

We analyze bulk service $M/G^{b}/1$ queues using the arrival time approach of Chae et al. (2001). As a result, the decomposition property of the M/G/1 queue with generalized vacations is extended to the $M/G^{b}/1$ queue in which the batch size is exactly a constant b. We also demonstrate that the arrival time approach is useful for relating the time-average queue length PGF to that of the departure time, both for the $M/G^{b}/1$queue in which the batch size is as big as possible but up to the maximum of constant b. The case that the batch size is a random variable is also briefly mentioned.

• Kyungpook Mathematical Journal
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• v.45 no.1
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• pp.45-53
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• 2005

A basis of the cycle space C (G) is d-fold if each edge occurs in at most d cycles of C(G). The basis number, b(G), of a graph G is defined to be the least integer d such that G has a d-fold basis for its cycle space. MacLane proved that a graph G is planar if and only if $b(G)\;{\leq}\;2$. Schmeichel showed that for $n\;{\geq}\;5,\;b(K_{n}\;{\bullet}\;P_{2})\;{\leq}\;1\;+\;b(K_n)$. Ali proved that for n, $m\;{\geq}\;5,\;b(K_n\;{\bullet}\;K_m)\;{\leq}\;3\;+\;b(K_n)\;+\;b(K_m)$. In this paper, we give an upper bound for the basis number of the semi-strong product of a bipartite graph with a cycle.

• Korean Journal of Mathematics
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• v.20 no.3
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• pp.285-291
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• 2012

Let $M_n$ be the algebra of all complex $n{\times}n$ matrices and ${\phi}:M_n{\rightarrow}M_n$ a surjective map (not necessarily additive or multiplicative) satisfying one of the following equations: $${\det}({\phi}(A){\phi}(B)+{\phi}(X))={\det}(AB+X),\;A,B,X{\in}M_n,\\{\sigma}({\phi}(A){\phi}(B)+{\phi}(X))={\sigma}(AB+X),\;A,B,X{\in}M_n$$. Then it is an automorphism, where ${\sigma}(A)$ is the spectrum of $A{\in}M_n$. We also show that if $\mathfrak{A}$ be a standard operator algebra, $\mathfrak{B}$ is a unital Banach algebra with trivial center and if ${\phi}:\mathfrak{A}{\rightarrow}\mathfrak{B}$ is a multiplicative surjection preserving spectrum, then ${\phi}$ is an algebra isomorphism.

• Korean Journal of Microbiology
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• v.28 no.2
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• pp.98-103
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• 1990

Brevibacterium lactofermentum SWA (arg trp) and B. lactofermentum SWB (met ser) were obtained from UV and NTG treatment. The rates of protoplast formation by B. lactofermentum SWA and SWB were 99.93% and 99.98%, respectively when each strain was treated with penicillin G in mid exponential growth phase, followed by incubation with 400 $\mu\textrm{g}$/ml of lysozyme in lysis fluid supplemented with 0.4M sucrose. Frequencies of protoplast regeneration in B. lactofermentum SWA and B. lactofermentum SWB were 9.27% and 10.32% respectively, on regeneration medium containing 0.5M sodium succinate, 50 mM $Mg^{2+}$, and 3% PVP. In intraspecific protoplast fusion between B. lactofermentum SWA and B. lactofermentum SWB, fusion frequency of $2.30\times 10^{-5}$ was observed by using the 100mM $CaCl_{2}$ and 30% PEG 6,000 in fusion fluid. Relative recombinant frequencies in each marker by means of selective media could be used for genetic analysis.

• Journal of Nutrition and Health
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• v.29 no.7
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• pp.772-777
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• 1996

The purpose of this study was to investigate the effect of vitamin B2 deficiency on the utilization and recuperation of fuel in exercising rats. Thirthy six rats were fed either a vitamin B2 deficient diet (-B2) or a control diet (+B2) for 3 weeks and then subdivided into 3 groups : non-exercise group(NE), exercise group (ES), exercise and recuperation group (ER). ES group were exercised on treadmill (10$^{\circ}$, 0.5-0.8km/h) for 2 hours and ER group were recuperated three days with the respective diet after exercise. Glucose (GLU), glycogen(GLY), protein(PRO), triglyceride(TG) and free fatty acid(FFA) were compared in plasma(P), liver(L) and skeletal muscle(M) of rats. Compared to + B2 rats, in NE group, the level of P-GLU and L-GLU of -B2 rats was lower, L-PRO and M-GLY was higher and there were no differences in P-PRO, P-FFA, L-TG and M-TG. In ES group, the level of P-GLU was lower, P-FFA was higher and there were no differences in P-PRO, P-TG, L-GLU, L-TG, M-GLY, M-TG and M-PRO. In ER groups, the level of P-GLU and L-TG was lower, P-FFA was higher and there were no differences in P-PRO, P-TG, L-GLY, L-PRO, M-GLY, M-TG and M-PRO. These results suggest that a vitamin B2 deficiency may impair the utilization of stored fuel during exercise suggest that a vitamin B2 deficiency may impair the utilization of stored fuel during exercise and may lead a sluggish recuperation related to fuel stores after exercise.

• Journal of Korean Society of Forest Science
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• v.105 no.4
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• pp.441-448
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• 2016

The purpose of this study was to broaden our knowledge on the productivity and costs of felling operation in three different harvesting methods(whole tree, tree-length and cut-to-length). Felling was conducted in three different harvesting methods with two workers who have different work experiences. Worker A and B have had felling experiences for about 5 years and 15 years, respectively. Felling productivity in whole tree method was $10.3m^3/SMH$ for worker A and $12.7m^3/SMH$ for worker B. Felling costs for worker A and B were $2,066won/m^3$ and $2,201won/m^3$, respectively. Although felling productivity of worker B in whole tree method was higher than worker A, felling costs of worker A were similar to worker B because the wage of worker B was more expensive than the wage of worker A (p>0.05). In tree-length method, felling cost of worker B were cheaper than that of worker A. Felling productivity and cost in tree-length method were $2.2m^3/SMH$ and $9,890won/m^3$ for worker A and $3.3m^3/SMH$ and $8,459won/m^3$ for worker B, respectively (p<0.05). In cut-to-length method, felling productivity and cost were $2.3m^3/SMH$ and $9,584won/m^3$ for worker A and $3.0m^3/SMH$ and $9,395won/m^3$ for worker B, respectively. Felling productivity of worker B was higher than that of worker A(p>0.05). Our preliminary results found that harvesting methods and worker's experiences highly affect on the productivity and costs of felling operations. These results should be useful for forest managers when planning cost-effective harvesting operations.

• Journal of Ecology and Environment
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• v.35 no.4
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• pp.313-322
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• 2012

A 2-year study (2010-2011) was carried out in order to evaluate the effects of bean and maize intercropping. The experiment tested five different cropping systems: sole cropping of each crop, as well as intercropping of maize/bean with the ratios of 1:3 ($M_1B_3$), 2:2 ($M_2B_2$) and 3:1 ($M_3B_1$), each of which took place in the presence of two weed management systems (no weed control and weed eradication through manual removal), in a factorial experiment based on randomized complete block design using three replicates. Tests of homogeneity of variance for combined data over two years showed that data of both years could be analyzed together. The results showed that the effect of intercropping treatments on all measured traits in maize and bean were significantly different. A minimum land equivalent ratio (LER) for maize (0.78) was obtained for $M_3B_1$ under conditions of no weed control, while the highest LER (1.03) was observed in $M_3B_1$ under weed-free conditions. The highest (0.99) and lowest (0.70) LER values for beans were recorded for $M_1B_3$ under weed-free conditions and $M_3B_1$ under conditions of no weed control, respectively. $M_1B_3$ under weed-free conditions showed the highest total LER (2.02), while $M_3B_1$ under conditions of no weed control showed the lowest (1.48). Results of this study indicated that intercropping bean and maize can be an effective method to increase total productivity, and that the $M_1B_3$ system was the best cropping system for high productivity.

• The Korean Journal of Pesticide Science
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• v.14 no.4
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• pp.326-331
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• 2010

Bacillus subtilis B29, B. subtilis M10 and Streptomyces sp. CC19 obtained from phyllosphere of cucumber plants were selected for biological control of fungal air-borne diseases. For the downy mildew, diseased area of B. subtilis B29, B. subtilis M10 and Streptomyces sp. CC19 showed 0.5%, 20.2% and 42.0%, but that of control was 82.0% respectively, in cucumber seedling test. Incidence of powdery mildew by once application of B. subtilis B29, B. subtilis M10 and Streptomyces sp. CC19 was 2.8%, 3.6% and 12.3%, respectively, whereas that of control was 65.6%. On the gray mold, diseased area of B. subtilis B29, B. subtilis M10 and Streptomyces sp. CC19 was 8.0%, 30.8% and 5.2%, respectively, compared to 81.2% for the control. Therefore, B. subtilis B29 could be a prospective antagonist for biological control of powdery mildew, downy mildew and gray mold of cucumber plant.