Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Calcium-Boron Interaction in Exopolysaccharide Production by the Cyanobacterium, Nostoc spongiaeforme

  • Singh (Centre of Advanced Study in Botany, Banaras Hindu University) ;
  • Netu (Centre of Advanced Study in Botany, Banaras Hindu University) ;
  • Asthana, R.K. (Centre of Advanced Study in Botany, Banaras Hindu University) ;
  • Singh, S.P. (Centre of Advanced Study in Botany, Banaras Hindu University)
  • Published : 2000.06.01
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Abstract

The effect and interaction of Ca and B on exopolysaccharide (EPS) synthesis in the diazotrophically growing cyanobacterium. Nostoc spongiaeforme, was investigated. The absence of B inhibited EPS synthesis 1.56-fold ($16\mu\textrm{g}$ glucose equivalent/mg dry weight, 16 d) over the control cells ($25\mu\textrm{g}$ glucose equivalent) grown in medium containing 0.5 mM Ca and $8{\mu}{\textrm}{m}$ B. When B concentration was raised to $40{\mu}{\textrm}{m}$, EPS production was stimulated 1.8-fold. Reduction of Ca concentraion to one-half (0.25 mM) resulted in increased B demand (16$\muM$) by the cells for EPS production at par with the normal sets. However, without Ca, EPS production also increased as B increased. Addition of B to a Ca-free medium stimulated cyanobacterial diazotrophic growth as well as synthesis of Chl a and phycocyanin (0-8 d). The data suggest B-dependent diazotrophic growth during Ca-deficiency and point to and important interactive role of Ca and B in regulation of cyanobacterial EPS synthesis.

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References

  1. Arch. Microbiol. v.69 Nitrogen chlorosis in blue-green algae. Allen, M. M.;A. J. Smith
  2. Plant Physiol v.80 Glyoxylate induced changes in the carbon and nitrogen metabolism of the cyanobacterium Anabaena cylindrica Bergman, B.
  3. Plant Physiol. v.94 Boron requirement in cyanobacteria. Its possible role in the early evolution of photosynthetic organisms Bonilla, I.;M. Garcia-Gonzalez;P. Mateo
  4. Physiologia Plant v.94 Interaction of boron and calcium in the cyanobacteria Anabaena and Synechococcus Bonilla, I.;L. Bolanos;P. Mateo
  5. Biochim. Biophys. Acta. v.50 A quantitative assay for the number of chromatophores on a chromoprotein: Its application to phycoerythrin and phycocyanin Brody, S. S.;M. Brody
  6. Trends Biotechnol. v.16 Polysaccharide biotechnology a Cinderella subject. Bucke, C.
  7. Planta v.173 Calcium requirement in nitrogen fixation in the cyanobacterium Synechococcus RF-I Chen, T. H.;H. Tan-Chi;C. Te-Jin
  8. Analytical Chem. v.28 Colorimetric method for determination of sugars and related substances Dubois, M.;K. A. Gilles;J. K. Hamilton;P. A. Rebers;F. Smith
  9. Plant Physiol v.87 Boron protection for O₂diffusion in heterocysts of Anabaena sp. PCC 7119 Garcia-Gonzalez, M.;P. Mateo;I. Bonilla
  10. Am. J. Bot. v.27 The isolation, purification and culture of blue-green algae Gerloff, G. C.;G. P. Fitzgerald;F. Skoog.
  11. Methods in Microbiology v.VB Chemical analysis of microbial cells Herbert, D.;P. J. Phipps;R. E. Strange;J. R. Norris(ed.);D. W. Ribbons(eds.)
  12. Plant Physiol. v.75 Evidence for the occurrence and possible physiological role for cyanobacterial calmodulin Kerson, G. W.;J. A. Miernyk;K. Budd
  13. J. Biol. Chem. v.193 Protein measurements with the Folin-phenol reagent Lowry, O. H.;N. J. Rosebrough;A. L. Farr;R. J. Randall
  14. Plant Physiol. v.81 Essentiality of boron for dinitrogen fixation in Anabaena sp. PCC 7119 Mateo, P.;I. Bonilla;E. Fernandez-Valiente;E. Sanchez-Maeso
  15. J. Gen. Physiol. v.39 Relationship between pigment content and photosynthetic characteristics in a blue-green alga Myers, J.;W. A. Kratz.
  16. Arch. Microbiol. v.150 Sulphated exopolysaccharides produced by two unicellular strains of cyanobacteria, Synechocystis PCC 6803 and 6714 Panoff, J. M.;B. Priem;H. Morvan;F. Joset
  17. Appl. Environ. Microbiol. v.57 Chemical characterization of polysaccharide from the slime layer of the cyanobacterium Microcystis flos-aquae C3-40. Plude, J. L.;D. L. Parker;O. J. Schommer;R. J. Timmerman;S. A. Hagstrom;J. M. Joers;R. Hnasko
  18. World J. Microbiol. Biotechnol. v.12 Phenotypic variation in exopolysaccharide production in the marine, aerobic nitrogen fixing unicellular cyanobacterium Cyanothece sp. Reddy, K. J.;B. W. Soper;J. Tang;R. L. Bradley
  19. Plant Physiol. v.92 Ca requirement for aerobic nitrogen fixation by heterocystous blue-green algae Rodriguez, H.;J. Rivas;M. G. Guerrero;M. Losada
  20. Indian J. Exp. Biol. v.36 Calciumdependent metabolic regulations in prokaryotes indicate conserved nature of calmodulin gene Sengupta, L. K.;B. B. Singh;R. Mishra;P. K. Pandey;S. Singh;S. Sengupta;P. S. Bisen
  21. Process Biochem. v.35 Thiol and exopolysaccharide production in a cyanobacterium under heavy metal stress Singh;Neetu;R. K. Asthana;A. M. Kayastha;S. Pandey;A. K. Chaudhary;S. P. Singh
  22. New Phytol v.105 Evidence for calcium-mediated regulation of heterocyst frequency and nitrogenase activity in Nostoc 6720 Smith, R. J.;S. Hobson;I. R. Ellis
  23. Plant Physiol v.66 Effect of boron deficiency on $^{86}rubidium$ uptake and photosynthesis in the diatom Cylindrotheca fusiformis Smith, D. A.;W. M. Dugger.
  24. Physiol. Plant. v.51 Cellular changes during boron deficient culture of the diatom Cylindrotheca fusiformis Smyth, D. A.;W. M. Dugger
  25. Trends Biotechnol v.15 Biopolymers from marine prokaryotes Weiner, R. M.