• Journal of Plant Biotechnology
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• 제49권4호
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• pp.325-330
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• 2022

본 연구는 국내에서 소비되는 약용작물 중 경제적 가치는 높으나 국내 생산량이 적고 수입 의존도가 높은 Atractylodes의 식물 조직 배양 시스템을 구축하기 위해 수행되었다. 삽주는 A. ovata를 사용하였고, 4가지 cytokinins류, 6-benzylaminopurine (BA), zeatin, kinetin, thidiazuron (TDZ)을 2가지 농도(0.5, 1.0 mg/L)로 처리하였다. 4가지 유형의 cytokinin 중 BA처리는 A. ovata의 신초유도와 뿌리 생육에 효과적이었다. 0.5 mg/L 및 1.0 mg/L BA 모두 BA 처리에서 유사한 결과를 나타내었지만 1.0 mg/L BA가 신초와 뿌리 생육을 촉진하는데 더 효과적이었다. 처리 중 신초의 개수와 뿌리의 생중량(FW)을 제외하고는 TDZ 처리가 신초와 뿌리생육에 효과적이지 않아 본 수종에서는 적합하지 않았다. 본 연구에서는 A. ovata의 아배양을 이용한 기내증식 시스템을 구축하였다. 위 결과는 기내 재분화를 이용한 산림약용자원 A. ovata의 안정적인 생산 및 증식을 위한 기반 기술로 사용될 것으로 사료된다.

• 생약학회지
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• 제12권1호
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• pp.23-25
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• 1981

Methanol extracts of the roots of Atractylodes japonica (alba) (Compositae) and Gentiana scabra (Gentianaceae) were further fractionated with n-butanol, chloroform, n-hexane and water. Then each fraction was p.o. administered to an animal model of hepatitis caused by $CCl_4$ intoxication. Measurements of sleeping time of mice induced by hexobarbital administration indicated that n-butanol and chloroform fractions of Atractylodes japonica (alba), and chloroform and n-hexane fractions of Gentiana scabra showed significant liver protective activities. Measurements of the activities of serum transaminase, S-GOT and S-GPT appeared to support these results.

• 동의생리병리학회지
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• 제18권2호
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• pp.596-598
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• 2004

The effects of water extract from Atractylodes macrocephala Koidz on biological activity were investigated. The crude water extract of A. macrocephala inhibited the growth of the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185, (3 mm inhibition zone at 300 ㎍/disc). However, it did not show growth inhibition activity against Sreptococcus mutans JC-2 (MIC >1,000 ㎍/mL). This extract was cytotoxic to P388 murine leukaemia cells ATCC CCL 46 P388D1, (IC/sub 50/ 62.24 ㎍/mL at 150 ㎍/disc). These results suggest that water extract of A. macrocephala possesses antitumoral, and antimicrobial activities.

• Food Science and Biotechnology
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• 제18권4호
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• pp.1019-1021
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• 2009

Antiproliferative activity of the ethanol extract of Atractylodes japonica rhizomes (AJEX) was investigated using methyl thiazolyl tetrazolium (MTT) assays with various cancer cell lines (HL-60, MCF-7, SK-Br-3, MDA-MB-453, HepG2, Hep3B, PC-3, LNCaP, MKN 28, MKN 45, and HT-29 cells). Gastric carcinoma cell lines were the most responsive in terms of cell proliferation. The $IC_{50}$ of MKN 28 and MKN 45 cells were 35.98 and 27.57 ${\mu}g/mL$, respectively. Moreover, gastric carcinoma cells exposed to AJEX underwent apoptosis, as determined by Annexin V binding assay. Compared to respective control level, exposure to the AJEX at each $IC_{50}$ concentration resulted in a remarkable increase in the shift of cell populations. Present results suggest that AJEX possess potential anticancer properties.

• 생약학회지
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• 제36권1호통권140호
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• pp.60-63
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• 2005

One sesquiterpenoid was isolated from the methanol extract of Atractylodes rhizomes which has been used as traditional medicine for diuresis and the compound was established as selina-4(14),7(11)-dien-8-one by the spectroscopic methods. The compound$(IC_{50}<10\;ppm)$ has inhibitory effects on melanogenesis in B16 mouse melanoma.

• 한국약용작물학회지
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• 제17권1호
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• pp.26-32
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• 2009

The region containing 18S rRNA gene, ITS 1 and part of the 5.8S rRNA gene of the Atractylodes japonica Koidz was amplified by PCR and the product cloned in a pBluescript SK II plasmid. DNA sequence of the cloned DNA was determined and submitted to the GenBank (accession number EU678363). Phylogenetic analysis of the ITS 1 DNA showed close similarity with the other plant species of the family Compositae. The extract of the plant materials of five different members of the family Compositae was analyzed by HPLC to detect atractylon. Extract of the A. japonica Koidz showed presence of significant amount of atractylon. However, noticeable amount of atractylon was not detected by the same analyses from the extracts of the other plants belonging to the family Compositae including Artemisia capillaris, Chrysantemum zawadskii, Eclipta prostrata or Taraxacum platycarpum.

• 한국자원식물학회지
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• 제24권5호
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• pp.591-598
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• 2011

본 연구는 국내에서 소비되는 약용작물 중 경제적 가치가 높은 백출의 조직배양체계 확립을 위해 수행되었다. 공시재료로는 중국백출(A. macrocephala)과 한국자생백출(A. japonica)의 교잡종인 '다출' 품종의 뿌리와 액아를 이용하였다. 뿌리배양을 위해 BAP, 2-iP, IBA, NAA를 혼용 처리한 결과, 캘러스 유도율은 BAP 처리군에서 다소 높았으나, 캘러스 증식 및 뿌리의 유도에는 2-iP 처리가 효과적이었다. 뿌리조직은 낮은 싸이토키닌의 농도와 높은 옥신의 농도에서 캘러스 유도 및 증식이 원활하였으나 식물체 재분화에는 적합하지 않았다. 백출의 액아배양 시 BAP와 NAA의 혼용처리가 2-iP와 NAA 혹은 IBA 처리보다 재분화식물체 생산에 효과적이었다. 1.0 mg/L BAP와 1.0 mg/L NAA 조건에서 액아 당 신초 출현 수가 3.8개로 가장 많았고, 경직경이 5.0 mm 이상으로 양호하였다. 그러나 BAP를 2-iP로 대치하는 경우 재분화 식물체의 초장은 큰 경향을 보이나 액아 당 재분화 식물체의 수가 적은 것으로 조사되었다. 또한 Sucrose 농도를 70 g/L로 하는 경우 액아로부터 재분화한 식물체의 뿌리 비대에 효과적이었다. 본 실험 결과, 백출의 기내 대량증식을 위한 조직으로는 뿌리보다 액아조직이 효율적이며, 식물의 재분화에는 1.0 mg/L BAP와 1.0 mg/L NAA 적합하고, 고농도의 sucrose가 뿌리 비대에 유용한 것으로 사료된다.

• 한국자원식물학회지
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• 제27권1호
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• pp.11-21
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• 2014

본 연구는 Atractylodes macrocepala KOIDZ.의 급성 경구 독성을 평가하기 위하여 SD계 Rat를 이용하여 농도별 열수 추출물을 투여하고 체중변화, 이상반응, 장기무게변화, 혈액학적 이상반응, 조직병리학적 이상반응 등을 측정하였다. Atractylodes macrocepala KOIDZ.를 투여한 실험군에서 비교적 낮은 농도에서 체중의 감소가 관찰되었으나, 이상반응이나 장기무게에 있어 유의성 있는 변화는 관찰되지 않았다. 혈액학적 지표에 있어서도 비교적 낮은 농도에서 WBC 및 AST의 증가가 관찰되었다. 조직병리학적 소견에서 일부 간조직의 지방변성이 관찰되어 간독성과 간세포에 미치는 영향에 대한 연구가 필요할 것으로 판단된다. 이와 같은 연구의 결과를 종합해 볼 때 백출이 경구투여에 있어 비교적 안전한 물질인 것으로 판단할 수 있다. 그러나 단회 경구투여 급성독성시험만으로 천연물 생약에 대한 독성 유무를 판단하기에는 일정부분 제약이 있으므로, 추가적으로 2주(또는 4주) 반복 경구투여 독성시험 및 13주 반복 경구투여 독성시험 그리고 유전독성에 대한 연구들이 순차적으로 수행되어야 하며, 이를 통하여 백출에 대한 체계적인 독성정보를 구축함으로써 보다 정확하고 과학적 근거에 입각한 안전성 자료가 확보될 수 있을 것으로 기대한다.

• 대한본초학회지
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• 제24권2호
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• pp.77-85
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• 2009

Objectives : To determine the discriminative criteria for Atractylodes rhizomes, the experiment of externalinternal characteristics and physicochemical pattern analysis were performed. Methods : External characteristics was observed using stereoscope. The sectioned materials which were covered with parffin were stained by Ju's method. Physicochemical patterns were analyzed using HPLC/DAD. Results : 1. External shape of original plant : Atractylodes maaocephala and A. japonica had relatively long petioles and 3-5 parted leaves. A. macrocephala had big purple flowers whereas A. japonica had relatively small white flowers and pinnate bracts. A. lancea had sessile leaves and white flowers, and the end parts of degenerated stamens were bent. 2. External shape of herbal medicine: A. macrocephala which was fist-shaped rhizome had pa-pillate processes and the cross section was light gray and sulcate. A. japonica and A. lancea were connected-beady or tubercular rhizomes, and the cross sections were both yellow-colored white. However, the cross section of A. japonica was fibrous, the width of cortex was narro-wer than that of stele, and radial shape in cortex was rare, whereas the width of cortex in A. lancea was similar to that of stele in size, and radial shape in cortex was obvious. 3. Internal shape of herbal medicine: A. macrocephala and A. lancea did not have lignified fascicles in cortex. However, the vascular bundles and vessels of A. macrocephala were wedge shaped and radial arrangement, and vascular bundles were densely populated in stele whereas those of A. lancea were repeatedly arranged and thinly extended to cortex. A. japonica had lignified fascicles in cortex and the width of vascular bundles was conspicuously thick with narrow intervals. 4. Physicochemical pattern analysis : A. macrocephala and A. lancea contained atractylenolide I and atractylenolide Ill whereas A. japonica contained atractylenolide I, atractylenolide Ill. diacetyl-atractylodiol, compound-4, compound-5. The three species of Atractylodes rhizomes showed different chromatogram patterns. Conclusions : The results could be used as discriminative criteria for Atractylodes rhizomes and as fundamental materials to researches of further pattern analysis and biological reaction.

• 한방안이비인후피부과학회지
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• 제36권4호
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• pp.30-50
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• 2023

Objectives : To investigate the active compounds and therapeutic mechanisms of Atractylodes Lancea(Thunb.) D.C. and Magnolia Officinalis Rehder et Wilson in the treatment of dermatitis accompanied by pruritus, as well as their potential to complement or replace standard drugs. Methods : We conducted the network pharmacological analysis. We selected effective ingredients among the active compounds of research target herbs. Then we explore pathway/terms of the common target proteins among research target herbs, fexofenadine and disease. Results : We selected 9 active compounds are selected from Atractylodes lancea and identified 231 target proteins. Among them, 74 proteins are associated with inflammatory skin diseases that cause pruritus. These proteins are involved in various pathways including, 'Nitric-oxide synthase regulator activity', 'Hydroperoxy icosatetraenoate dehydratase activity, Aromatase activity', 'RNA-directed DNA polymerase activity', 'Arachidonic acid metabolism', 'Peptide hormone processing', 'Chemokine binding' and 'Sterol biosynthetic process'. Additionally, coregenes are involved in 'IL-17 signaling pathway'. Similarly, we selected 2 active compounds from Magnolia officinalis and identified 133 target proteins. Among them, 33 proteins are related to inflammatory skin diseases that cause pruritus. These proteins are primarily involved in 'Vascular associated smooth muscle cell proliferation' and 'Arachidonic acid metabolism'. There is no significant difference between the pathways in which coregenes are involved. Conclusions : It is expected that Atractylodes Lancea will be able to show direct or indirect anti-pruritus and anti-inflammatory effects on skin inflammation accompanied pruritus through suppressing inflammation and protecting skin barrier. Meanwhile, it is expected that Magnolia Officinalis will only be able to show indirect anti-inflammation effects. Therefore, Atractylodes Lancea and fexofenadine are believed to complement each other, whereas Magnolia Officialinalis is expected to provide supplementary support on skin disease.