Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

An efficient in vitro shoot regeneration system for Atractylodes ovata (Thunb.) DC.

삽주(Atractylodes ovata (Thunb.) DC.)의 효율적인 기내 줄기 재분화

  • Hui Yeong Jeong (Forest Medicinal Resources Research Center, NIFoS) ;
  • Ji Ah Kim (Forest Medicinal Resources Research Center, NIFoS)
  • 정희영 (국립산림과학원 산림약용자원연구소) ;
  • 김지아 (국립산림과학원 산림약용자원연구소)
  • Received : 2022.10.11
  • Accepted : 2022.11.14
  • Published : 2022.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, a plant tissue culture system was established for Atractylodes spp., an economically valuable medicinal crop in Korea that has low domestic production and is increasingly imported. In particular, Atractylodes ovata was treated with four types of cytokinins, 6-benzylaminopurine (BA), zeatin, kinetin, and thidiazuron (TDZ), in two different concentrations (0.5 and 1.0 mg/L). Among the four types of cytokinins, the BA treatment was effective for the shoot and root growth of A. ovata. Both the 0.5 mg/L and 1.0 mg/L concentrations of BA showed similar results; however, the 1.0 mg/L concentration of BA was more effective in promoting shoot and root growth. The treatments showed that the TDZ treatment was not effective for the shoot and root growth, except for the number of shoots and the fresh weight (FW) of the root; therefore, it was unsuitable for this species. In this study, we established a mass production system of A. ovata. Our results showed that direct in vitro regeneration may make a significant contribution to improving the cultivation of the medicinal plant A. ovata.

본 연구는 국내에서 소비되는 약용작물 중 경제적 가치는 높으나 국내 생산량이 적고 수입 의존도가 높은 Atractylodes의 식물 조직 배양 시스템을 구축하기 위해 수행되었다. 삽주는 A. ovata를 사용하였고, 4가지 cytokinins류, 6-benzylaminopurine (BA), zeatin, kinetin, thidiazuron (TDZ)을 2가지 농도(0.5, 1.0 mg/L)로 처리하였다. 4가지 유형의 cytokinin 중 BA처리는 A. ovata의 신초유도와 뿌리 생육에 효과적이었다. 0.5 mg/L 및 1.0 mg/L BA 모두 BA 처리에서 유사한 결과를 나타내었지만 1.0 mg/L BA가 신초와 뿌리 생육을 촉진하는데 더 효과적이었다. 처리 중 신초의 개수와 뿌리의 생중량(FW)을 제외하고는 TDZ 처리가 신초와 뿌리생육에 효과적이지 않아 본 수종에서는 적합하지 않았다. 본 연구에서는 A. ovata의 아배양을 이용한 기내증식 시스템을 구축하였다. 위 결과는 기내 재분화를 이용한 산림약용자원 A. ovata의 안정적인 생산 및 증식을 위한 기반 기술로 사용될 것으로 사료된다.

Keywords

Acknowledgement

본 연구는 산림청 국립산림과학원 연구개발사업(과제번호: FG0700-2018-02)의 지원에 의해 수행되었음.

References

  1. Animal and Plant Quarantine Agency (2021) Quarantine record on importing plants. 2021 Year book of plant quarantine statics. p226
  2. Bizeng M, Bowei H, Zaiming C, Bingliang W, Huifeng P, Debao L (2009) Effects of plant growth regulators on the rapid proliferation of shoots and root induction in the Chinese traditional medicinal plant Atractylodes macrocephala. Frontiers of Biology in China. 4: 217-221 https://doi.org/10.1007/s11515-009-0006-9
  3. Demir S, Celike FG (2019) Effects of plant growth regulators on plant height and quantitative properties of Narcissus tazetta. Turk J Agric For 43:105-114 https://doi.org/10.3906/tar-1802-106
  4. Fratini R, Ruiz ML (2002) Comparative study of different cytokinins in the induction of morphogenesis in Lentil (Lens Culinaris Medik). In Vitro Cellular & Developmental Biology-Plant 38:46-51 https://doi.org/10.1079/IVP2001252
  5. Jun X, Fu P, Lei Y, Cheng P (2018) Pharmacological effects of medicinal components of Atractylodes lancea (Thunb.) DC. Chinese Medicine 13(1):1-10 https://doi.org/10.1186/s13020-017-0157-6
  6. Koo WL, Cho JH, Park CG, Ahn YS, Park CB (2011) Effect of plant growth regulators on in vitro cultured Atractylodes hybrid 'Dachul' (A. macrocephala X A. japonica). Korean Journal of Plant Resources 24(5):591-598 https://doi.org/10.7732/kjpr.2011.24.5.591
  7. Korea Forest Service (KFS) (2018) Production Survey of Forest Products Current Status of Production and Production Prices by Item. 2017 Production of Forest Products. p.52
  8. Korea Forest Service (KFS) (2021) Production Survey of Forest Products Current Status of Production and Production Prices by Item. 2020 Production of Forest Products. p.23
  9. Lim H, Lee JH, Kim JW, Kim YS, Kim HP (2012) Effects of the Rhizomes of Atractylodes japonica and Atractylenolide I on Allergic Response and Experimental Atopic Dermatitis. Arch Pharm Res 35(11):2007-2012
  10. Miller WB (2012) Current status of growth regulator usage in flower bulb forcing in north america. Floric Ornament Biotechnol 6:35-44
  11. National Institute of Horticultural and herbal science (NIHHS) (2016) Atractylodes ovata. Guideline for GAP cultivation technology of medicinal crops. pp.128-141
  12. Noboru H, Jung In C, Indra Dutt B (2003) Cold storage of Atractylodes ovata shoot cultures and evaluation of the regenerated plants. Plant Biotechnology 20(4):347-351 https://doi.org/10.5511/plantbiotechnology.20.347
  13. Song G, Xu Y, Shi J, Xie Z (2018) Establishment of large-scale tissue culture and rapid propagation system for Atractylodes lancea. Acta Agriculturae Jiangxi 30(9):63-67
  14. Yukihiro S, Reiko T, Itsuo N (1987) Clonal Multiplication of Atractylodes lancea by Tip Tissue Culture. Shoyakugaku Zasshi 41(4):313-317
  15. Yuling L, Liangyan X, Wenwen L, Yuanyuan Z (2020) Direct shoot regeneration in vitro for Atractylodes macrocephala using epicotyl and radicleas explant. Journal of Hebei University, DOI:10.3969/j.issn.1000 1565.2020.02.009
  16. Yun PS, Ning ZH, Chuan MF, Feng LT, Zhi GZ (2010) Optimization of proliferation culture medium of Atractylodes lancea (Thunb.) DC. subsp. luotianensis S. L. Hu et X. F. Feng. Medicinal Plant 1(11):18-20
  17. Zhu YQ, Xia GH, Fang HG, Fu SH, He FJ (2006) Study on tissue culture and rapid propagation of Atractylodes macrocephala. Journal of Chinese medicinal materials 29(3):212-3
  18. Yun BR, Weon JB, Lee B, Lee J, Eom MR, Ma CJ (2013) Quantitative analysis of atractylenolides I and III in Atractylodes japonica. Korean Journal of Pharmacognosy 44(1):53-59
  19. Zhang WJ, Zhao ZY, Chang LK, Cao Y, Wang S, Kang CZ, Wang HY, Zhou L, Huang LQ, Guo LP (2021) Atractylodis Rhizoma: A review of its traditional uses, phytochemistry, pharmacology, toxicology and quality control. Journal of Ethnopharmacology 266:113415
  20. Zhu B, Zhang Q, Hua JW, Cheng W, Qin LP (2018) The traditional uses, phytochemistry, and pharmacology of Atractylodes macrocephala Koidz.: a review. Journal of Ethnopharmacology 226:143-167 https://doi.org/10.1016/j.jep.2018.08.023