• Proceedings of the Korean Society of Crop Science Conference
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• 2021.04a
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• pp.116-116
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• 2021

• Proceedings of the Korean Society of Crop Science Conference
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• 2021.04a
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• pp.117-117
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• 2021

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.5
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• pp.626-635
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• 2009

An experiment was conducted to investigate the effect of Butylated Hydroxy Anisole (BHA), Butylated Hydroxy Toluene (BHT), Pentoxifylline (PTX), Theophylline (TPY) and Theobromine (TBR) on cold protection ability of Murrah buffalo semen at room ($22-25^{\circ}C$) and refrigerated temperature ($4-7^{\circ}C$). Each semen sample was divided into six parts of equal volume and sperm concentration; the first was kept as a control and the remaining five were treated with BHA, BHT, PTX, TPY or TBR. Sperm motility, abnormal spermatozoa, live-dead count, hypo-osmotic swelling and acrosomal integrity were studied at room and refrigerated temperature for various incubation periods viz.; 0, 4, 8, 12 and 24 h at room and 0, 12, 24, 36, 48, 60 and 72 h at refrigerated temperature. Significant improvement in sperm motility, live-dead count, hypo-osmotic swelling and acrosomal integrity were observed in BHT, PTX and TPY fortified extender at room and refrigerated temperature for various incubation periods. From the present study it could be concluded that cold protection ability of buffalo semen can be improved through the addition of BHT followed by PTX and TPY.

• Korean Journal of Environment and Ecology
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• v.27 no.6
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• pp.666-675
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• 2013

This research was carried out from March 2009 to July 2012 to explicate the height change based breeding-ecological characteristics of Titmice that were breeding in the artificial nests installed in the Halla Mountain. The first egg-laying day was delayed as the height of the area under investigation increased, and it turned out that the decrease in temperature due to the elevated height had influence on that delay (p<0.05). Generalizing the annual results of the analyses, we see that there was no significant correlation occurring between the average temperatures and egg-laying days in the breeding season (April~June) (p>0.05). The rate of artificial nest use was not different among areas and among year, but between the species, Parus major and Parus varius. There were differences in clutch sizes among species (p>0.05), but no differences in heights and years (p<0.05). The hatching-, fledging-, and breeding-success rates were 67.3%, 99.5%, and 67.0% respectively in 2011, and 71.3%, 96.8%, 69.0% respectively in 2012, from which we can tell that the fledging-success rate was high but the hatching- and breeding-success rates were similar. Though we confirm that temperature changes due to height differences had influence on breeding periods, but we cannot identify a significant correlation with other breeding-ecological characteristics. Thus, in order to more precisely explicate the influence of temperature changes due to heights on Titmice's breeding, long-term research is needed in terms of the investigation of microclimatic factors and food resource.

• Animal Bioscience
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• v.34 no.12
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• pp.1879-1885
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• 2021

Objective: Owing to the lack of a breeding index for efficient and quick fertility evaluations of Holstein bulls when using traditional or genome-wide detection methods, this study aimed to determine whether in vitro fertilization (IVF) could be used as an indicator of conception rate of artificial insemination (AI). Methods: Conventional and sexed frozen semen from nine bulls were used for IVF and AI. Results: The IVF and AI conception rates of each bull were confirmed to be positively correlated between the conventional frozen and sexed frozen semen. The correlation coefficient R values of nine bulls between IVF and AI methods were 0.73 and 0.97 for the conventional frozen and sexed frozen semen, respectively. The average conception rate of three bulls undergoing AI was 69.5% and 64.2%, 61.8% and 58.8%, and 48.2% and 46.2% in first-, second-, and third-born cows when conventional frozen and sexed frozen semen were used, respectively, which showed a positive correlation with the fertilization rate in the same parity. We propose an evaluation standard to assess the fertilization ability of bulls based on their IVF test results, which is categorized into three grades: grade one, normal fertility bull with an AI conception rate of 40%±5% and IVF rate of 45% to 60%; grade two, higher fertility bull with an AI conception rate of 50%±5% and IVF rate of 61% to 80%; and grade three, highest fertility bull with an AI conception rate of 60%±5% and IVF rate of >80%. Conclusion: These findings reveal that IVF results can be used as a breeding index for bulls to evaluate their AI conception ability, which may shorten the time required to select bulls for breeding.

• Journal of Practical Agriculture & Fisheries Research
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• v.25 no.3
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• pp.35-46
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• 2023

It was in the 1982 that artificial seed production research for olive flounder (Paralichthys olivaceus) farming was first conducted in Korea (Currently, National Institute of Fisheries Science, Fish Breeding Research Center). In 1985, fertilized eggs were obtained from natural olive flounder adapted to land tanks, and artificial seed production technology was established and fertilized eggs were distributed. In the late 1980s, halibut aquaculture began to prosper in land-based tank farming in Jeju Island and Busan's Gijang region, where water temperatures are relatively high in winter. Currently, aquaculture is being carried out all over the country, centering on Jeju Island and Wando, Jeollanam-do. However, olive flounder farming, which started with a small group in the 1980s, reduced genetic diversity through inbreeding over generations, resulting in side effects such as slow growth, reduced resistance to disease and environmental conditions. In order to solve these genetic problems of farmed olive flounder in Korea, the Fish Breeding Research Center of the National Institute of Fisheries Science introduced a wild-caught parent fish group to the existing aquaculture group from 2003 to 2004. Genetic diversity was secured and KingNupchi with fast growth and improved body shape was developed. In this study, the current status of breeding technology development of olive flounder, a major aquaculture breed in Korea, is reviewed and future research directions are suggested.

• Journal of Embryo Transfer
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• v.24 no.3
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• pp.139-143
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• 2009

Most captive canids and felids at Zoos in advanced countries have been examined enough to apply artificial reproductive techniques to them. We investigated reproductive hormones and vaginal epithelial cells of a 6-year-old, female coyote, hoping these data could eventually be extended to artificial insemination with frozen-thawed conspecific semen at Seoul Zoo. As a relative of pet dogs, coyote exhibited a similar appearance with only minor differences. In vaginal smear, an increase in the number of superficial cells suggests that the bitch has reached a state close to estrus. A sudden decrease of estradiol and increase of progesterone is considered as a preovulatory event. Vaginal epithelial cells and hormones might be useful for determining the optimal time of artificial insemination in coyotes' breeding.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.23 no.4
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• pp.97-110
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• 2020

This study conducted observations at 72 traffic lights and road signs in Sejong-si, Korea from March to June 2019 in order to analyze nest-site, nest-site characteristics and feeding behavior in Tree sparrow (Passeer montanus) in urban. Feeding behavior during breeding season in 2019 was investigated at 10 Town representing traffic facilities installed on the road located in the center of the city and 10 Riverside Geumgang river's riverside road. We fitted both general linear model and regression model to our field data and estimated the spatial variation of initial date of the first feeding (i.e., being date of breeding) and the total number of caterpillar fed by parents per hour. Our results showed that the frequency of nest building on artificial structures, such as traffic lights, was about 26% higher and about 8 days earlier in the riverside. In particular, the first feeding was significantly faster when the nearest distance between nest to green field as foraging site was shorter whereas there was no significant effect of the number of nest within 50 meters on the first feeding. Number of caterpillars fed by parents was higher in the Riverside than in the Urban, and especially the number in the second brood was sharply decreased in the Urban. As we estimated average amount of caterpillars per hour to 446 traffic safety facilities in Sejong-si, it was estimated that Tree sparrows fed 18,742.8 caterpillars per hour during the breeding season. In this study, we showed that characteristics of nests built in artificial structures and feeding behavior in order to analyze effects of environmental condition in urban on life-history traits during the breeding season. Finally, we discussed the conservation of birds in urban and pest control effects.

• Journal of Korean Society of Forest Science
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• v.97 no.1
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• pp.83-87
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• 2008

This study was conducted to clarify the use of artificial nest boxes of tits in coniferous and deciduous forests from March to June 2006 and 2007. Tree species richness, tree species diversity and total basal area were higher in deciduous forest than in coniferous forest. Except the bush-ground layer (<1 m), the other vertical layers were more developed in deciduous forest. Varied tit Parus varius, marsh tit P. palustris, coal tit P. ater and major tit P. major used the artificial nest boxes in this study. Number of breeding pairs of tits were higher in deciduous than in coniferous forest. Clutch size, brood size and breeding success of coal tits were higher in coniferous forest. Brood size and breeding success of major tits were higher in deciduous forest. Characteristics of habitat and niche partition would be influenced on tits' use of artificial nest boxes. The more detailed researches on artificial nest boxes would be needed for management and conservation of birds.

• Korean journal of applied entomology
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• v.46 no.1 s.145
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• pp.123-129
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• 2007

An artificial insemination instrument of bumblebee queens was firstly developed. This instrument consists of location tube, transfer tube, holding tube, head product, and probe apparatus for reproductive tract etc. This instrument was designed to minimize stress and damage of reproductive tract of bumblebee queens. The regulator handle apparatus in artificial insemination instrument was used the principle of lever, that manipulates easy, accurate and rapid insemination of bumblebee queens. By using this instrument, the insemination rate was over 90%. This instrument will be useful for the breeding and conservation of excellent character of bumblebees.