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http://dx.doi.org/10.5713/ajas.2009.70267

Effect of Antioxidant Preservative on Cold Protection Ability of Low Grade Riverine Buffalo (Bubalus bubalis) Bull Spermatozoa  

Pankaj, Prabhat Kumar (Artificial Breeding Complex, National Dairy Research Institute)
Raina, V.S. (Artificial Breeding Complex, National Dairy Research Institute)
Roy, B. (Dept. of LPM, College of Veterinary Science & A.H)
Mohanty, T.K. (Artificial Breeding Complex, National Dairy Research Institute)
Mishra, A. (Dept. of Vety. Physiology, College of Veterinary Science & A.H.)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.22, no.5, 2009 , pp. 626-635 More about this Journal
Abstract
An experiment was conducted to investigate the effect of Butylated Hydroxy Anisole (BHA), Butylated Hydroxy Toluene (BHT), Pentoxifylline (PTX), Theophylline (TPY) and Theobromine (TBR) on cold protection ability of Murrah buffalo semen at room ($22-25^{\circ}C$) and refrigerated temperature ($4-7^{\circ}C$). Each semen sample was divided into six parts of equal volume and sperm concentration; the first was kept as a control and the remaining five were treated with BHA, BHT, PTX, TPY or TBR. Sperm motility, abnormal spermatozoa, live-dead count, hypo-osmotic swelling and acrosomal integrity were studied at room and refrigerated temperature for various incubation periods viz.; 0, 4, 8, 12 and 24 h at room and 0, 12, 24, 36, 48, 60 and 72 h at refrigerated temperature. Significant improvement in sperm motility, live-dead count, hypo-osmotic swelling and acrosomal integrity were observed in BHT, PTX and TPY fortified extender at room and refrigerated temperature for various incubation periods. From the present study it could be concluded that cold protection ability of buffalo semen can be improved through the addition of BHT followed by PTX and TPY.
Keywords
Buffalo Semen; Sperm Motility; Non-Eosinohilic; Butylated Hydroxy Anisole; Butylated Hydroxy Toluene; Pentoxifylline; Theophylline; Theobromine;
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