Changes of Estradiol, Progesterone and Vaginal Epithelial Cells of a Pseudopregnant Captive Coyote (Canis latrans) during Breeding Season

  • Published : 2009.09.30

Abstract

Most captive canids and felids at Zoos in advanced countries have been examined enough to apply artificial reproductive techniques to them. We investigated reproductive hormones and vaginal epithelial cells of a 6-year-old, female coyote, hoping these data could eventually be extended to artificial insemination with frozen-thawed conspecific semen at Seoul Zoo. As a relative of pet dogs, coyote exhibited a similar appearance with only minor differences. In vaginal smear, an increase in the number of superficial cells suggests that the bitch has reached a state close to estrus. A sudden decrease of estradiol and increase of progesterone is considered as a preovulatory event. Vaginal epithelial cells and hormones might be useful for determining the optimal time of artificial insemination in coyotes' breeding.

Keywords

References

  1. Bekoff M and Wells MC. 1986. Social ecology and behavior of coyotes. Advances in the Study of Behavior 16:251-338 https://doi.org/10.1016/S0065-3454(08)60193-X
  2. Carlson DA and Gese EM. 2007. Relaxin as a diagnostic tool for pregnancy in the coyote. Anim. Reprod. Sci. 101:304-312 https://doi.org/10.1016/j.anireprosci.2006.07.011
  3. Carlson DA and Gese EM. 2008. Reproductive biology of the coyote (Canis latrans): integration of mating behavior, reproductive hormones, and vaginal cytology. J. Mammol. 89:654-664 https://doi.org/10.1644/06-MAMM-A-436R1.1
  4. Feldman EC and Nelson RW. 2004. Canine and Feline Endocrinology and Reproduction. 3rd ed. Saunders, St. Louis, Missouri
  5. Gese EM. 2001 Territorial defense by coyotes (Canis latrans) in Yellowstone National Park, Wyoming: who, how, where, when, and why. Can. J. Zool. 79:980-987 https://doi.org/10.1139/cjz-79-6-980
  6. Goodrowe KL, Mastromonaco GF, Walker SL, Bateman HL, Rychman DP, Platz CC Jr and Waddell WT. 2001. In vitro maintenance, cooling and cryopreservation of red wolf (Canis rufus) spermatozoa. J. Reprod. Fertil. Suppl. 57:387-392
  7. Green JS, Knowlton FF and Pitt WC. 2002. Reproduction in captive wild-caught coyotes (Canis latrans). J. Mammol. 83:501-506 https://doi.org/10.1644/1545-1542(2002)083<0501:RICWCC>2.0.CO;2
  8. Hase M, Hori T, Kawakami E and Tsutsui T. 2000. Plasma LH and progesterone levels before and after ovulation and observation of ovarian follicles by ultrasonographic diagnosis system in dogs. J. Vet. Med. Sci. 62:243-248 https://doi.org/10.1292/jvms.62.243
  9. Hodges CM. 1990. The reproductive biology of the coyote (Canis latrans). Ph. D. dissertation, Texas A&M University, College Station
  10. Kennelly JJ and Johns BE. 1976. The estrous cycle of coyotes. J. Wildl. Man. 40:272-277 https://doi.org/10.2307/3800425
  11. Kennelly JJ, Johns BE, Breidenstein CP and Roberts JD. 1977. Predicting female coyote breeding dates from fetal measurements. J. Wildl. Man. 41:746-750 https://doi.org/10.2307/3800000
  12. Knowlton FF, Gese ER and Jaeger MM. 1999. Coyote depredation control: and interface between biology and management. J. Ran. Man. 52:398-412 https://doi.org/10.2307/4003765
  13. Mech LD. 1970. The Wolf: The Ecology and Behavior of an Endangered Species. Natural History Press, Garden City, New York
  14. Shin YJ, Son JM, Lim YH, Kim YS, Lee DS, Yoon KY, Shin ST and Cho JK. 2008. Effects of glycerol concentration on viability of frozen-thawed canine spermatozoa. J. Emb. Trans. 23:115-118
  15. Stellflug JN, Muse PD, Everson DO and Louis TM. 1981. Changes in serum progesterone and estrogen of the nonpregnant coyote during the breeding season. Proceedings of the Society for Experimental Biology and Medicine 167:220-223 https://doi.org/10.3181/00379727-167-41153
  16. Thomassen Rand Farstad W. 2009. Artificial insemination in canids: a useful tool in breeding and conservation. Theriogenology 71:190-199 https://doi.org/10.1016/j.theriogenology.2008.09.007
  17. Zindl C, Asa CS and Gunzel-Apel AR. 2006. Influence of cooling rates and addition of Equex pasta on cooled and frozen-thawed semen of generic gray (Canis lupus) and Mexican gray wolves (C. I. baileyi). Theriogenology 66:1797-1802 https://doi.org/10.1016/j.theriogenology.2006.01.023