• 한방안이비인후피부과학회지
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• 제31권4호
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• pp.1-12
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• 2018

Objectives : This study investigated the anti-oxidant and anti-inflammatory activities of Changchulgeumryeontang (CCGRT) extract. Methods : The macrophage cell line RAW 264.7 cells were used and MTT assay was performed to measure the cell viabilities at the various concentrations of CCGRT ($25-200{\mu}g/m{\ell}$). Nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) were measured in LPS-induced RAW 264.7 cells. Expressions of iNOS, $NF-{\kappa}B$, $IL-1{\alpha}$, $IL-1{\beta}$ and IL-6 were also performed by real-time PCR. The anti-oxidant activities of CCGRT was measured by DPPH radical scavenging activity. Results : 1. there was no cytotoxicity in RAW264.7 cells treated with CCGRT compared to the control. 2. CCGRT treated group significantly inhibited NO and $PGE_2$ production compared to the LPS treated group. 3. CCGRT treated group significantly decreased mRNA expressions of iNOS, $NF-{\kappa}B$, $IL-1{\alpha}$, $IL-1{\beta}$ and IL-6 compared to the LPS treated group. 4. CCGTR was found to have high DPPH free radical scavenging ability. Conclusions : According to the above results, CCGRT may be a potentional choice for the treatment of inflammatory skin disease. Conclusions : According to the above results, CCGRT may be a potentional choice for the treatment of inflammatory skin disease.

• 한국염색가공학회:학술대회논문집
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• 한국염색가공학회 2011년도 제45차 학술발표회
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• pp.41-41
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• 2011

The aims of this study were to examine the efficacy of phytochemicals of Rumex crispus as anti-oxidant and anti-browning agent. The bioactive properties of Rumex crispus as natural colorants were studied by total phenolic contents, DPPH radical scavenging, ABTS radical scavenging,lipid peroxidation, cell viability, singlet oxygen quenching and photoprotection effect. Among all of the results ($IC_{50}$: the concentration of various extracts required to exert 50% reducing effect), the higher activity of the extract was found in the ethyl acetate and butanol extracts. Anti-browning activity was evaluated by monitoring the change $L^*$, $a^*$, $b^*$ values and total color differences(${\Delta}E$). It was found that ethyl acetate and butanol extracts effectively inhibited browning in apple juice at a concentration below 0.3 mg/ml. Rumex crispus extracts used natural colorants could be of good resources as anti-oxidant and anti-browning agents. The results suggest that our study may contribute to the development of natural and functional materials with potential application to reduce oxidative damage.

• 한국응용과학기술학회지
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• 제33권4호
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• pp.762-770
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• 2016

본 연구는 구절초 꽃 추출물이 기능성 화장품 소재로서의 개발 가능성을 관찰하기 위하여 항산화 활성과 피부세포에 대한 세포독성, 항염증, 멜라닌 생합성 억제능에 미치는 영향을 관찰하였다. 본 연구 결과 구절초 꽃 추출물에서 폴리페놀과 플라보노이드의 높은 함량과 뛰어난 DPPH radical 소거작용을 확인하였고, Raw 264.7세포와 B16F10세포에 대해 유의한 세포독성을 나타나지 않았으며, Raw 264.7세포에서 LPS에 의해 유도된 NO 생성을 유의하게 억제함으로써 항염증 효과를 확인하였다. 구절초 꽃 추출물이 B16F10세포에 ${\alpha}$-MSH로 멜라닌 생성을 유도한 후 멜라닌 생합성 억제능을 측정한 결과 멜라닌의 생성 증가를 농도 의존적으로 억제되는 것을 확인하였다. 이와 같은 결과로 보아 구절초 꽃 추출물은 세포에 대한 독성이 낮고, 높은 항산화 활성과 항염증, 미백효과를 가진 기능성 화장품소재로써의 활용 가능성이 있을 것으로 사료된다.

• International journal of advanced smart convergence
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• 제7권2호
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• pp.38-46
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• 2018

This study proceeded an experiment that can increase such physiological properties of heated radish extract. During the extraction of radish, including the byproduct, an increase in antioxidant properties of radish according the heating temperature was performed. Upon the extracts of radish bark and radish green extract(mucheong), the physiological functionalities and antioxidant activity were investigated. As a result, the color of radish ethanol extract in dependence of heating temperature, showed light brown color at low heating temperature and black color from $150^{\circ}C$. The total polyphenol content significantly increased as a result of heat treatment; 6.7 times and 22 times higher than the control at $110^{\circ}C$ and $150^{\circ}C$, respectively. DPPH radical scavenging ability and antioxidant property increased with increasing heating temperature; in comparison to heat-treated radish at $110^{\circ}C$ and $150^{\circ}C$, $IC_{50}$ decreased by 1/22 times. $IC_{50}$ of the control was 23times higher than $150^{\circ}C$ heat treated radish (Control $IC_{50}$:130.305). According to the graph that represents ABTS activity, antioxidant activity increased in dependence of heat treatment likewise to the total polyphenol content and DDPH radical scavenging activity. Upon heat treatment at $150^{\circ}C$, antioxidant activity in consequence of ABTS assay increased 23 times higher than the control.

• 한국버섯학회지
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• 제19권1호
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• pp.41-50
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• 2021

국내 자생하는 야생버섯 추출물의 생리활성 성분을 평가하기 위하여 각지에서 수집된 야생버섯 70% 에탄올추출물에 대한 DPPH 라디컬 소거능, 총 폴리페놀 및 총 플라보노이드 함량, 철 환원 항산화능, 환원력의 항산화 활성, 아질산염 소거능 및 야생버섯의 건조시료를 이용하여 베타글루칸 함량을 분석하였다. 본 연구에서 수집된 10종의 야생버섯류 중에서 영지(OK1362) 에탄올추출물의 DPPH 라디컬 소거능(73.2%), 총 폴리페놀 함량(28.9 mg GAE/g) 및 총 플라보노이드 함량(10.0 mg QE/g)철 환원 항산화능(0.134), 환원력(0.155), 아질산염 소거능(56.3%)이 다른 버섯류에 비하여 유의적으로 가장 높았다. 그 이외에도 아까시흰구멍버섯(OK1360), 광대버섯속의 A. lanigera (OK1398), 졸각무당버섯(OK1406)이 높은 항산화능 및 아질산염 소거능을 가지고 있음을 확인하였다. 또한 베타글루칸 함량은 영지(OK1362)가 25.2%를 나타내며 가장 높았으며, 그 외에 구름버섯속의 T. lactinea (OK1457)가 24.5%로 높은 것으로 나타났다. 본 연구의 결과는 국내 자생하는 야생버섯류 중 새로운 천연물 유래 생리활성 물질을 탐색하기 위한 기초자료로서 활용 가능성을 기대한다.

• 융합정보논문지
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• 제11권12호
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• pp.236-243
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• 2021

본 연구는 자색당근(black carrot, BC) 소재로 초임계추출물에 대해 DPPH radical 소거능, ABTS radical 소거능, 총 폴리페놀 함량 측정을 통해 항산화 활성, 인간의 정상 섬유아세포에서 콜라겐 생성과 MMP-1 발현을 조사하여 항주름 효능을 평가하고자 하였다. 그 결과 자색당근 초임계추출물 내 총 폴리페놀 함량 (9.037±1.123 mg GAE/g), DPPH, ABTS 분석 결과로 높은 항산화 효과를 확인하였다. NHDF 세포에서 초임계추출물 농도에 의한 세포독성은 거의 없었으며, 이때 추출물은 농도 의존적으로 MMP-1 단백질 및 MMP-1 mRNA 발현을 억제하고, 콜라겐 type I 유전자 mRNA 수준 증가에 의한 type I procollagen의 합성을 촉진하였다. 이상의 결과를 종합해 볼 때, 자색당근 초임계추출물은 우수한 항산화 효능과 친환경(solvent free)물질, 항노화 원료로서 화장품, 식품, 미용식품 등 다양한 산업분야에 있어 활용 가능성이 매우 높을 것으로 판단된다.

• Biomolecules & Therapeutics
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• 제27권4호
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• pp.381-385
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• 2019

We attempted to examine anti-inflammatory and anti-oxidant effects of 4'-O-${\beta}$-D-glucosyl-5-O-methylvisamminol (GOMV), the first epigenetic inhibitor of histone phosphorylation at Ser10. While GOMV did not affect the viability of murine macrophage RAW 264.7 cells, it significantly suppressed lipopolysaccharide (LPS)-induced generation of prostaglandin $E_2$ ($PGE_2$) and nitric oxide (NO) through transcriptional inhibition of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). GOMV also scavenged free radicals in vitro, increased NF-E2-related factor 2 (NRF2), and activated antioxidant response element (ARE), thereby resulting in the induction of phase II cytoprotective enzymes in human keratinocyte HaCaT cells. Finally, GOMV significantly protected HaCaT cells against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced oxidative intracellular damages. Together, our results illustrate that GOMV possesses anti-inflammatory and anti-oxidant activity.

• Natural Product Sciences
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• 제18권4호
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• pp.261-265
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• 2012

Xanthohumol was isolated from hops (Humulus lupulus L.), and then investigated anti-oxidant effect by AAPH-induced LLC-PK1 cell and oxygen radical absorbance capacity (ORCA) assays and MMP-12 inhibitory effect by direct MMP-12 inhibition assay. The treatment of xanthohumol protected LLC-PK1 cells from AAPH-induced cell damage such as cell viability, SOD and GSH-px reduction in a dose dependant manner (0.1, 1, and $5{\mu}M$), the SOD value was 2.98, 4.51, and 5.77 U/mg protein, and GSH-px value was 30.12, 49.32, and 60.11 U/mg protein. ORAC value of xanthohumol was showed as 4320, 12004, and $14209{\mu}M$ TE/g at the concentration 0.1, 1, and $5{\mu}M$, respectively. The change of SOD and GSH-px values was significantly correlated with the results of ORAC assay, that is, AAPH-induced cell and ORCA assays. In addition, inhibition of MMP-12 that is known to play an important role in skin aging was 14%, 37%, 46%, and 79% at the concentration of 0.01, 0.1, 1, and $5{\mu}M$, respectively. On the basis of these results, xanthohumol from hops (Humulus lupulus L.) showed interesting biological and pharmacological activity such as anti-oxidant effect and anti-aging.

• 생약학회지
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• 제49권1호
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• pp.7-14
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• 2018

We isolated gallotannin, 2,6-digalloyl-1,5-anhydroglucitol, known as acertannin (1), from the barks and xylems of Acer ginnala Maxim. It is a genus of Acer species of shrubs in the family Aceraceae. A. ginnala grows in Korea, Japan and Mongolia. We accomplished the structure elucidation by confirming that the result of $^1H$,$^{13}C-NMR$,MS spectrum data was similar to previous references. We measured DPPH and ABTS radical scavenging activity in vitro to evaluate anti-oxidative activities on acertannin isolated from A. ginnala. Acertannin from A. ginnala exhibited potent DPPH and ABTS radical scavenging activities. We examined the antioxidant and apoptosis modulative effects. This examination shows that A. ginnala has not only 1,1-diphenyl-2-picryhydrazyl(DPPH) radical scavenging activity and ABTS radical scavenging activity, but also human hair dermal papilla cell protection effects. These results indicate that the barks and xylems of A. ginnala might be developed as a potent anti-oxidant, hair growth agent, and ingredient for related new functional cosmetic materials.

• 한국식품영양학회지
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• 제34권5호
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• pp.553-559
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• 2021

The aim of study to investigate the phytochemicals and biological activities the bark of Betula schmidtii. The studies consisted of the solvent extraction, followed by the isolation of phenolic components 1~3 from ethyl acetate-soluble fraction of Betula schmidtii Bark. Their chemical structures were identified as arbutin (1), ρ-coumaric acid (2) and ferulic acid (3) using Ultraviolet-Visible (UV-Vis) Spectrophotometer, Electrospray Ionization Mass Spectrometry (ESI-MS) (negative ion mode), ¹H-Nuclear Magnetic Resonance (NMR), ¹³C-NMR, ¹H-¹H Correlation Spectroscopy (COSY) and ¹H-¹³C Hetero Nuclear Multiple Quantum Correlation (HMQC) spectral data. Compounds 1~3 shows the anti-oxidant effect with IC₅₀ values of 29.74±1.52, 21.32±1.07 and 34.41±1.24 in 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, respectively. Also, compounds 1~3 exhibited mushroom tyrosinase inhibitory activity with IC₅₀ values of 31.14±1.07, 42.54±1.46 and 69.22±1.43 µM, respectively.