Browse > Article
http://dx.doi.org/10.9799/ksfan.2021.34.5.553

Identification of Anti-Oxidant and Anti-Tyrosinase Activity of Phenolic Components Isolated from Betula schmidtii  

Wang, Da-Hye (Dept. of Chemistry, College of Natural Sciences, Duksung Women's University)
Chung, Ha Sook (Dept. of Food and Nutrition, College of Natural Sciences, Duksung Women's University)
Publication Information
The Korean Journal of Food And Nutrition / v.34, no.5, 2021 , pp. 553-559 More about this Journal
Abstract
The aim of study to investigate the phytochemicals and biological activities the bark of Betula schmidtii. The studies consisted of the solvent extraction, followed by the isolation of phenolic components 1~3 from ethyl acetate-soluble fraction of Betula schmidtii Bark. Their chemical structures were identified as arbutin (1), ρ-coumaric acid (2) and ferulic acid (3) using Ultraviolet-Visible (UV-Vis) Spectrophotometer, Electrospray Ionization Mass Spectrometry (ESI-MS) (negative ion mode), 1H-Nuclear Magnetic Resonance (NMR), 13C-NMR, 1H-1H Correlation Spectroscopy (COSY) and 1H-13C Hetero Nuclear Multiple Quantum Correlation (HMQC) spectral data. Compounds 1~3 shows the anti-oxidant effect with IC50 values of 29.74±1.52, 21.32±1.07 and 34.41±1.24 in 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, respectively. Also, compounds 1~3 exhibited mushroom tyrosinase inhibitory activity with IC50 values of 31.14±1.07, 42.54±1.46 and 69.22±1.43 µM, respectively.
Keywords
Betula schmidtii Bark; phenols; DPPH free radical scavenging; mushroom tyrosinase; phytochemicals;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Xu WH, Liang Q, Zhang YJ, Zhao P. 2015. Naturally occurring arbutin derivatives and their bioactivities. Chem Biodivers 12:54-81   DOI
2 Yang EJ, An JH, Son YK, Yeo JH, Song KS. 2018. The cytotoxic constituents of Betula platyphylla and their effects on human lung A549 cancer cells. Nat Prod Sci 24:219-224   DOI
3 Zhang HM, Wang CF, Shen SM, Wang GL, Liu P, Liu ZM, Wang YY, Du SS, Liu ZL, Deng ZW. 2012. Antioxidant phenolic compounds from Pu-erh tea. Molecules 17:14037-14045   DOI
4 Zhou H, Zhao J, Li A, Reetz MT. 2019. Chemical and biocatalytic routes to arbutin. Molecules 24:3303   DOI
5 Bae EH. 2014. Biopharmaceutics Trend Report. Korea Bio-Economy Research Center
6 Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181:1199-1200   DOI
7 Chung HS. 2001. Isolation of new bioactive phytochemicals from natural products. Food Ind Nutr 6:53-59
8 Eom HJ, Shin HY, Ji YM, Kwon NR, Yoon HS, Kim IJ, Song Y, Yu KW. 2021. Nutritional components and physiological activity of Centella asiatica cultured in Chungju by drying method. Korean J Food Nutr 34:165-173   DOI
9 Flurkey WH. 1991. Identification of tyrosinase in mushrooms by isoelectric focusing. J Food Sci 56:93-95   DOI
10 Hong JJ, Seol HG, Oh JY, Jeong EH, Chang YH. 2021. Effective component contents and antioxidative activities of unripe apple by extraction methods. Korean J Food Nutr 34:174-180   DOI
11 Kim SH, Jeon BJ, Kim DH, Kim BR, Lee KY, Kim TB, Park JH, Kim YC, Sung SH. 2010. Cognitive enhancing activity of Betula platyphylla sap in scopolamine induced amnesic mice. Nat Prod Sci 16:50-53
12 Le DD, Nguyen DH, Zhao BT, Min BS, Song SW, Woo MH. 2019. Quantitation and radical scavenging activity evaluation of iridoids and phenylethanoids from the roots of Phlomis umbrosa (Turcz.) using DPPH free radical and DPPH-HPLC methods, and their cytotoxicity. Nat Prod Sci 25:122-129   DOI
13 Menkovic N, Savikin K, Tasic S, Zdunic G, Stesevic D, Milosavljevic S, Vincek D. 2011. Ethnobotanical study on traditional uses of wild medicinal plants in Prokletije Mountains (Montenegro). J Ethnopharmacol 133:97-107   DOI
14 Rastogi S, Pandey MM, Rawat AKS. 2015. Medicinal plants of the genus Betula-Traditional uses and a phytochemical-pharmacological review. J Ethnopharmacol 159:62-83   DOI
15 Woo WS. 2001. Study on Natural Products Chemistry. Seoul National University Press
16 Guon TE, Chung HS. 2016. Hyperoside and rutin of Nelumbo nucifera induce mitochondrial apoptosis through a caspase-dependent mechanism in HT-29 human colon cancer cells. Oncol Lett 11:2463-2470   DOI
17 Zdunska K, Dana A, Kolodziejczak A, Rotsztejn H. 2018. Antioxidant properties of ferulic acid and its possible application. Skin Pharmacol Physiol 31:332-336   DOI
18 Kim JY, Kim JY, Jenis J, Li ZP, Ban YJ, Baiseitova A, Park KH. 2019. Tyrosinase inhibitory study of flavonolignans from the seeds of Silybum marianum (Milk thistle). Bioorg Med Chem 27:2499-2507   DOI
19 Siah M, Farzaei MH, Ashrafi-Kooshk MR, Adibi H, Arab SS, Rashidi MR, Khodarahmi R. 2016. Inhibition of guinea pig aldehyde oxidase activity by different flavonoid compounds: An in vitro study. Bioorg Chem 64:74-84   DOI
20 Deans BJ, Kilah NL, Jordan GJ, Bissember AC, Smith JA. 2018. Arbutin derivatives isolated from ancient Proteaceae: Potential phytochemical markers present in Bellendena, Cenarrhenes, and Persoonia genera. J Nat Prod 81:1241-1251   DOI
21 Han BH, Chi HJ. 1974. Triterpenoids of Betua latifolia KOMAROV. Korean J Pharmacogn 5:45-47
22 Jeong HS. 2001. Isolation of new bioactive phytochemicals from natural products. Food Ind Nutr 6:53-59
23 Kim SR, Kim YC. 2000. Neuroprotective phenylpropanoid esters of rhamnose isolated from roots of Scrophularia buergeriana. Phytochemistry 54:503-509   DOI
24 King A, Young G. 1999. Characteristics and occurrence of phenolic phytochemicals. J Acad Nutr Diet 99:213-218
25 Loub WD, Farnsworth NR, Soejarto DD, Quinn ML. 1985. NAPRALERT: Computer handling of natural product research data. J Chem Inf Comput Sci 25:99-103   DOI
26 Maeda K, Fukuda M. 1996. Arbutin: Mechanism of its depigmenting action in human melanocyte culture. J Pharmacol Exp Ther 276:765-769
27 Migas P, Krauze-Baranowska M. 2015. The significance of arbutin and its derivatives in therapy and cosmetics. Phytochem Lett 13:35-40   DOI
28 Naczk M, Shahidi F. 2004. Extraction and analysis of phenolics in food. J Chromatogr A 1054:95-111   DOI
29 Sroka Z, Cisowski W. 2003. Hydrogen peroxide scavenging, antioxidant and anti-radical activity of some phenolic acids. Food Chem Toxicol 41:753-758   DOI