• Journal of Applied Biological Chemistry
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• 제60권3호
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• pp.219-226
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• 2017

본 연구에서는 탁리산 열수추출물을 용매 분획하여 항산화, 주름억제, 미백효과에 대한 활성을 검증하여 화장품 소재로서 이용가능성을 연구하였다. 용매 분획물로 DPPH radical scavenge, ABTS radical scavenge, elastase, tyrosinase 저해활성을 확인하였고 에틸아세테이트 분획물(TW-EA)이 가장 높은 저해활성을 나타내었다. 세포내에서의 주름억제, 미백효과를 확인하기 위해서 western blot을 실시하였고 비교 검증을 위해 사용한 positive contol보다 TW-EA의 억제효과가 뛰어난 것을 확인하였다. 이러한 결과로 탁리산의 화장품소재로서 적용 가능성을 검증하였다.

• 대한본초학회지
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• 제21권4호
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• pp.159-170
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• 2006

Objectives : Anti-oxidation activities of Malvae Semen and Abutili Semen were compared and evaluated. Methods : We tested the anti-oxidant effect through in vitro experiment and in vivo experiment that induced oxidative stress using ethanol. Results : 1. In the anti-oxidative activities of Malvae Semen and Abutili Semen they had high effects in case of the scavenging activitie of DPPH and superoxide anion radical, and inhibitory effect of linoleic acid oxidation. 2. Malvae Semen had large quantities of phenolic compound than Abutili Semen, but the anti-oxidative activity of Malvae Semen was weaker than that of Abutili Semen. 3. The anti-oxidative effect of Abutili Semen was more strong than that of Malvae Semen in the rat stressed by ethanol. Conclusion : Anti-oxidation activities of Abutili Semen were stronger than those of Malvae Semen. Therefore, the complementary usage of Malvae Semen and Abutili Semen can be considered.

• 한국응용과학기술학회지
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• 제35권1호
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• pp.43-54
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• 2018

본 연구에서는 삼칠화 에탄올 추출물의 항산화 및 피부 세포 독성, 항염증을 확인하고, 삼칠화 함유 화장품을 여드름 피부에 적용하여 수분, 홍반, 모공, 블랙헤드에 미치는 영향을 분석하여 화장품 소재로서의 가능성을 규명하고자 하였다. 연구 결과, 삼칠화 에탄올 추출물의 DPPH radicial 소거능을 확인하였고, 높은 폴리페놀 함량과 플라보노이드 함량을 나타내었다. 삼칠화 에탄올 추출물은 RAW 264.7 세포에 대한 독성이 적었고, LPS에 의해 유도된 NO 생성을 유의하게 억제하는 것을 확인하였다. 4주간 삼칠화 에탄올 추출물이 3% 함유된 스킨 토너와 스팟 솔루션을 여드름 피부에 적용한 결과, 수분 증가와 홍반지수, 눈에 띄는 모공수, 블랙헤드 감소가 유의하게 나타났고, 사용 후 만족도 조사에서 피지량 감소, 붉음증 완화에 대한 만족도가 높은 것을 확인하였다. 따라서 삼칠화는 항산화 및 항염증 효과가 있으며, 여드름 관리용 화장품 소재로서 활용 가능할 것으로 사료된다.

• 셀메드
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• 제13권2호
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• pp.2.1-2.9
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• 2023

CPS219, a new concept compound composed of coffee (CO), Pueraria Tomsonii flower (PF), and Sambou bamboo salt^TM (BS), was developed as a coffee beverage to alleviate menopausal symptoms. The purpose of this study is to evaluate the anti-oxidant and menopausal alleviating effects of CPS219 combined as the optimal ratio of each component selected through sensory evaluation and blind consumer test. For CPS219, the optimal ratio of CO, PF, and BS was determined to be 1:0.1:0.017 through various sensory evaluations and blind consumer tests. CPS219 significantly enhanced the superoxide dismutase-like activity compared to the CO or CO plus PF (CP). The proliferation of MCF-7 cells was considerably increased after 24 hours by treatment with CO, CP, or CPS219, but only CPS219 significantly boosted the proliferation of MCF-7 cells after 48 hours. Moreover, CPS219 had an estrogen-like effect by dramatically increasing the expression of estrogen receptor-β mRNA in MCF-7 cells but not CO and CP. Treatment of MCF-7 cells with CO, CP, or CPS219 did not cause any cytotoxicity. In conclusion, these findings imply that anti-oxidant and estrogen-like properties of CPS219 can be used to prevent and cure postmenopausal symptoms.

• 한국염색가공학회:학술대회논문집
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• 한국염색가공학회 2012년도 제46차 학술발표회
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• pp.122-122
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• 2012

The aims of this study were to examine the efficacy of phytochemical compounds of colorants as anti-oxidant agent. The bioactive properties of natural colorants were studied by total phenolic contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and anti-bacterial activity in Escherichial coli. The concentration ($IC_{50}$) of various extracts from colorants required to exert 50% reducing effect on DPPH radical were found to be 0.007-4 mg/ml. Especially, the gallnut (Schlectendalia Chinensis) displayed remarkable effect of DPPH as compared to positive control ascorbic acid. The total phenolic contents (TPC) and restraint of E coli. also analyzed. It was found that gallnut extracts effectively inhibited DPPH radical at a concentration below 0.01 mg/ml. Natural colorant extracts could be of good resources as anti-oxidant and anti-bacterial agents. The results suggest that our study may contribute to the development of natural and functional materials with potential application to reduce oxidative damage.

• 생약학회지
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• 제52권4호
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• pp.242-250
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• 2021

Artemisia frigida Willd (AW, Fringed sagewort), which is widespread in Mongolia, is a well-known medicinal plant as a member of the Compositae family. This study aims to explore the gastroprotective effect of water extract of AW on 150 mM HCl/60% ethanol-induced acute gastritis in 5 week old male ICR mice. Total polyphenols, total flavonoid contents, and anti-oxidant activity in vitro in AW were evaluated. First, the gross area of gastric mucosal damage was measured. Then western blot analysis was conducted to determine the possible mechanisms of action underlying the effects of AW. AW administration decreased gastric mucosal damage. Moreover, the group with AW treatment effectively inhibited nicotinamide adenine dinucleotide phosphate (NADPH) oxidase expression associated with oxidative stress. AW treatment enhanced an anti-oxidant effect through the increase of anti-oxidant proteins. Besides, the increased expressions of inflammatory cytokines induced by nuclear factor-kappa B (NF-κB) activation are alleviated through AW treatment. Taken together, AW exerted a gastroprotective effect against gastric mucosal damage. These results indicate that AW could have the potential used as a natural therapeutic drug for the treatment of acute gastritis.

• KSBB Journal
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• 제26권6호
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• pp.483-490
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• 2011

Bioactive peptides (BAP) showed excellent cosmetic activity than bio-materials such as caffeic acid (CA), gallic acid (GA), and nicotinic acid (NA). Caffeoyl tripeptide-1 (CT-1) is a BAP that is stabilized with Gly-His-Lys (GHK) tripeptide and CA by using Fmoc solid phase peptide synthesis. Digalloyl tetrapeptide-19 (DT-19) is stabilized by combining Lys-Glu-Cys-Gly with GA and nicotinoyl tripeptide-1 (NT-1) is synthesized by GHK and NA. According to experiments, CT-1 has an excellent anti-oxidant function even with a very small amount of 10 ppm CT-1. DT-19's tyrosinase inhibition activity has the better effect of about 28.57% in 0.01% and 33.33% in 0.005% of concentration and about 7.89% in 0.001% concentration than vitamin-C. In addition, NT-1 is safer than the NA. Almost BAPs like pal-KTTKS, acetyl hexapeptide, and copper tripeptide-1 have the anti-wrinkle effect while DT-19 and NT-1 are applicable for potential BAPs focused on the whitening effect. The three kinds of BAPs like CT-1, DT-19, and NT-1 consisting of amino acids are safe to the skin, and have more excellent stability than bio-materials which are found to be unstable and cause skin irritation. Due to the high biological activity of BAP in the field of skin care, its utilization will increase constantly.

• 생약학회지
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• 제49권2호
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• pp.138-144
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• 2018

Asparagus oligoclonos is one of the endemic halophytes used folk medicine in Korea. We isolated the main compound rutin from methanol extracts of A. oligoclonos based on nuclear magnetic resonance and TOF ESI-MS data. We have investigated the quantitative analysis method of main compound using HPLC and the results exhibit that rutin content of A.oligoclonos were 1.816%. To explore anti-oxidant from A. oligoclonos ethanol extracts (AOE), we investigated the antioxidant effects of AOE on $H_2O_2$-induced oxidative stress in RAW 264.7 cell. AOE were reduced $H_2O_2$-induced oxidative stress via enhancement of cell viability, and AOE significantly decreased ROS production depending on concentration. Next, to screen for anti-inflammatory activity of AOE, we investigated the inhibitory effects of AOE in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. AOE had no effect on cell viability at a concentration of $500{\mu}g/mL$. Nitric oxide (NO) production inhibited in a dose-dependent manner. These results suggest that AOE may be a useful antioxidant and anti-inflammatory agent.

• 한국식품과학회지
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• 제36권1호
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• pp.129-133
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• 2004

배추김치의 dichloromethane 획분의 수율은 약 1.66% 이었으며, 이로부터 silica gel column chroamtography, Sepadex LH-20 column chromatography, Sep-pack catridge 등을 이용하여 활성물질을 분리 정제하였고, IR, EI-MS, GC-MS. $^{1}H-NMR,\;^13C-NMR$로 구조를 확인하여 분자량 226인 3-(4'-hydroxyl-3',5'-dimethoxyphenyl)propionic acid로 밝혀다. 비추김치 활성성분의 DPPH 유리기 소거능은 vitamin C에 비해 3.4배 높았고, LDL 산화억제 효과는 대조군에 비해 현저히 높아 인체에서 지질저하 효과 및 동맥경화 억제 효과가 있을 것으로 예상된다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권10호
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• pp.4129-4133
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• 2014

Background: The objective was to study the effect of Scutellaria baicalensis Georgi ethanol extracts (SBGE) on immune and anti-oxidant function in U14 tumor-bearing mice. Materials and Methods: U14 tumor-bearing mice were randomly divided into eight groups: a control group, a cyclophosphamide (CTX) group, three dose groups of SBGEI (high, medium, low), and three dose groups of SBGEII (high, medium, low). After two weeks, the thymus and spleen weight indices of mice bearing U14 cervical cancer were calculated. Enzyme linked immunosorbent assays (ELISA) was used to determine the levels of serum IL-2, TNF-${\alpha}$, IL-8, and PCNA. MDA activity and SOD activity in plasma were measured with detection kits. Results: In the SBGE groups, thymus weight and spleen weight indices of U14 tumor-bearing mice were significantly higher than in the control group or CTX group (p<0.05). Compared to control group, the levels of serum IL-2 and TNF-${\alpha}$ in U14 tumor-bearing mice increased significantly, whereas the contents of serum IL-8 and PCNA decreased (p<0.05). The activity of SOD increased with the growing dose of SBGE, while the activity of MDA decreased significantly in the highe-rdose groups of SBGE. Conclusions: These findings suggested that SBGE, especially at high dose, 1000 mg/kg, showed significant immune and anti-oxidant effects infU14 tumor-bearing mice, which might be the mechanisms of SBGE inhibition of tumor growth.