Browse > Article

Quantitative Analysis of Chemical Composition and In Vitro Anti-oxidant and Anti-inflammatory Properties of Asparagus oligoclonos  

Lee, Hyun Joo (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
Jeong, Da Eun (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
Gang, Ju Eun (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
Sim, Mi-Ok (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
Seong, Tae Kyoung (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
Woo, Kyeong Wan (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
An, Byeongkwan (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
Jung, Ho Kyung (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
Cho, Hyun Woo (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
Publication Information
Korean Journal of Pharmacognosy / v.49, no.2, 2018 , pp. 138-144 More about this Journal
Abstract
Asparagus oligoclonos is one of the endemic halophytes used folk medicine in Korea. We isolated the main compound rutin from methanol extracts of A. oligoclonos based on nuclear magnetic resonance and TOF ESI-MS data. We have investigated the quantitative analysis method of main compound using HPLC and the results exhibit that rutin content of A.oligoclonos were 1.816%. To explore anti-oxidant from A. oligoclonos ethanol extracts (AOE), we investigated the antioxidant effects of AOE on $H_2O_2$-induced oxidative stress in RAW 264.7 cell. AOE were reduced $H_2O_2$-induced oxidative stress via enhancement of cell viability, and AOE significantly decreased ROS production depending on concentration. Next, to screen for anti-inflammatory activity of AOE, we investigated the inhibitory effects of AOE in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. AOE had no effect on cell viability at a concentration of $500{\mu}g/mL$. Nitric oxide (NO) production inhibited in a dose-dependent manner. These results suggest that AOE may be a useful antioxidant and anti-inflammatory agent.
Keywords
Liliaceae; Asparagus oligoclonos; Rutin; Anti-oxidant activity; ROS; Anti-inflammation;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Sukito, A. and Tachibana, S. (2014) Isolation of hyperoside and isoquercitrin from Camellia sasanqua as antioxidant agents. Pak. J. Biol. Sci. 17: 999-1006.   DOI
2 Ferreira, O. and Pinho, S. P. (2012) Solubility of flavonoids in pure solvents. Ind. Eng. Chem. Res. 51: 6586-6590.   DOI
3 Kwak, C. S. and Choi, H. I. (2015) In vitro antioxidant and anti-inflammatory activities of ethanol extract and sequential fractions of flowers of Prunus persica in LPS-stimulated RAW 264.7 macrophages. J. Korean Soc. Food Sci. Nutr. 44: 1439-1449.   DOI
4 Yi, M. R., Kang, C. H. and Bu, H. J. (2017) Anti-inflammatory and tyrosinase inhibition effects of amaranth (Amaranthus spp L.) seed extract. Korean J. Plant Res. 30: 144-151.   DOI
5 Lee, S. W., Kim, K. N., Baek, N. I. and Han, J. T. (2001) Rutin, Antioxidant compound isolated from the fruit of Prunus menu. J. Korean Soc. Agric. Chem. Biotechnol. 44: 35-37.
6 Kazlowska, K., Hsu, T., Hou, C. C., Yang, W. C. and Tsai, G. J. (2010) Anti-inflammatory properties of phenolic compounds and crude extract from Porphyra dentata. J. Ethnopharmacol. 128: 123-130.   DOI
7 Zhou, Y. F., Guo, B., Ye, M. J., Liao, R. F. and Li, S. L. (2016) Protective effect of rutin against $H_2O_2$-induced oxidative stress and apoptosis in human lens Epithelial cells. Curr. Eye Res. 41: 933-942.   DOI
8 Cho, J. Y., Park, S. Y., Shin, M. J., Gao, T. C., Moon, J. H. and Ham, K. S. (2010) Isolation and identification of antioxidative compounds in fermented glasswort (Salicornia herbacea L.) Juice. J. Korean Soc. Food Sci. Nutr. 39: 1137-1142.   DOI
9 Aiyegoro, O. A. and Okoh, A. I. (2010) Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complement Altern. Med. 10: 1-8.   DOI
10 경보, 황선웅, 강종국, 이수환, 신평, 최원영, 김선, 이장희, 이휘종, 김형민 (2012) 한국의 해안 및 간척지 식물, 131. 농촌진흥청 국립식량과학원.
11 Kim, K. S., Seong, J. D., Park, S. Y., Oh, S. L. and Kwak, Y. H. (2000) Steroidal saponins from the rhizomes of Asparagus oligocolonos and their antibacterial activity. J. Korean Soc. Agric. Chem. Biotechnol. 43: 136-140.
12 Kim, K. S. (2006) Studies on anti-demential compounds of Asparagus oligoclonos and Rhus chinensis. ph D thesis. Kyungpook National University, Daegu, Korea.
13 Selvaraj, K., Chowdhury R. and Bhattacharjee, C. (2013) Isolation and structural elucidation of flavonoids from aquatic fern Azolla Microphylla and evaluation of free radical scavenging activity. Int. Pharm. Pharm. Sci. 5: 743-749.
14 Kim, S. J., Heo, M. Y., Son, K. H. and Kim, H. P. (2003) Tyrosinase inhibitory activity of 80 plant extracts. J. Appl. Pharmacol. 11: 5-7.
15 Cho, J. Y., Yang, X., Park, K. H., Park, H. J., Park, S. Y., Moon, J. H. and Ham, K. S. (2013) Isolation and identification of antioxidative compounds and their activities from Suaeda japonica. Food Sci. Biotechnol. 22: 1547-1557.   DOI