• 농촌의학ㆍ지역보건
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• 제15권1호
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• pp.3-8
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• 1990

Seven specimens of salmon(Oncorhynchus keta) purchased directly, in Oct. 10. 1990, in the Taep'o port. Kang-won-do were examined for infection state of anisakid larvae and classification of extracted larvae according to morphological characters. The results were as follows. 1) From seven salmon 202 anisakid larvae were found, and mean infection number of anisakid larvae per individual salmon was 28.86. 2) From total extracted anisakid larvae 198 larvae(98%) were found in muscle. Therefore the distribution of anisakid larvae in intestine was extremely rare compared to in muscle. 3) The percentage of anisakid larvae in II, IV muscle region was 93% and from this we could surmise that most anisakid larvae in salmon did not penetrate to the very distanted muscle from intestine. 4) Three types of anisakid larvae(Anisakis Type I, Contracaecum Type B, Contracaecum Type D) were identified and, among them, Contracaecum Type B was the first recording type in Korea 5) Larvae of Contracaecum it genus were found only in intestine. Therefore it surmised that penetration neture to muscle of Contracaecum larvae was less than that of Anisakis Type I.

• 농촌의학ㆍ지역보건
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• 제13권1호
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• pp.32-40
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• 1988

Anisakiasis occur after the ingestion of raw marine fish and squid containg anisakid larvae. In recent years about 40 cases of anisakiasis have been recorded in Korea. Considering, however, the Korean style of eating raw fish, many more cases would exist and prevention is necessary. We describe the infection rate of anisakid larvae in 13 species of marine fish and squid which were caught in the Korean sea. And each extracted larva is classified according to morphological characters. The results are following ; Scomber japonicus, Pseudosciaena manchurica, Trichiurus haumela showed high infection rate of anisakid larvae. Although Sepia esculenta showed low infection rate, most of anisakid larvae found in Sepia edulis are embedded in muscles. So relative high frequent rate of anisakiasis may developed by Sepia esculenta. Five type (Anisakis Type I, Terranova Type B, Raphidascaris sp., Contracaecum Type A, Contracaecum Type D) of anisakid larvae are classified according to their morphological characters.

• 농촌의학ㆍ지역보건
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• 제15권2호
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• pp.89-96
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• 1990

The results of infection state of anisakid larvae and parasitic helminths in salmon and sea-trout which caught from Taep'o port. Kang-won do were as follows. 1) From twelve specimens of sea-trout. 122 individuals of anisakid larvae were found and mean infection number of anisakid larvae per individual sea-trout was 10.17. 2) From total extracted anisakid larvae from sea-trout. 37 larvae(30.3%) were found in the muscle. This percentage was lower than that of salmon. but higher than that of other sea-fishes. 3) The taxonomic list of parasitic helminths which found in salmon and sea-trout was as follows. Class Trematoda Family Hemiuridae l. Brachyphallus crenatus(Rudolphi. 1802) 2. Lecithaster salmonis Yamaguti. 1934 Class Cestoda Family Phllobothriidae 3. Pelichnibothrium speciosum Monticelli, 1889-larval form Family Tentacuariidae 4. Tentacularia sp. - larval form 4) Among those 4 species of helminths. B.crenatus. L.salmonis, P.speciosum were the first recording species in Korea.

• 한국어병학회지
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• 제23권1호
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• pp.123-129
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• 2010

2006년부터 2008년까지 국내로 회귀하는 연어(O. keta)의 소상위치, 암수 및 근육 부위별 아니사키스형 유충의 감염상을 조사하였다. 3년 동안강에서 채집된 연어 17마리와 바다에서 채집된 13마리의 아니사키스형 유충의 감염율을 조사한 결과, 강에서 채집된 연어의 수컷 및 암컷은 마리 당 아니사키스형 유충이 각각 평균 $98{\pm}27$ 및 $103{\pm}27$ 마리씩 감염되어져 있었다. 바다에서 채집된 연어의 수컷 및 암컷은 마리 당 아니사키스형 유충이 각각 평균 $63{\pm}18$ 및 $108{\pm}17$ 마리씩 감염되어져 있었고, 암수 및 소상위치간의 아니사키스형 유충의 감염 마리수에서 통계학적 차이는 없는 것으로 나타났다. 아니사키스형 유충의 근육 부위별로 조사하면 복강내측근육 (abdominal muscle)에 85%가 기생하며, 내장기관에는 거의 유충이 발견되지 않았다. 동정된 아니사키스형 유충은 Anisakis type이 74%며, 나머지는 Contracacecum type로 분류되었다. 연어 근육내에서 아니사키스형 유충의 저온처리에 따른 생존율을 조사하였을시에 최소 6시간 이상의 냉동보존 ($-20^{\circ}C$)만이 아니사키스형 유충이 죽는다는 사실을 알 수 있었다.

• 한국환경보건학회지
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• 제26권2호
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• pp.67-69
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• 2000

Yellow tails (Seriola quinqueradiata) were purchased from the Jakalchi fish market located in Busna and examined for anisakid larvae. The collected larvae were classified by their morphologicaltypes. Of the 16 yellow tails exmained, seven (43.8%) were infected with the larvae. Among 90 larvae, 81 were collected from the omentum and 9 from the muscle. Anisakids were classified into five larval types, anisakis type I (56 larvae in number, 62.2%), Contracaecum type A (8, 8.9%) and type D (7, 7.8%) and Contracaecum type C (19, 21.1%). The present study revealed that Anisakis type I was the most common among the five larval types in the yellow tails caught in the South sea of Korea peninsula.

• 한국환경보건학회지
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• 제23권1호
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• pp.14-17
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• 1997

A total of 93 specimens of Astroconger myriaster were purchased from the Chungmu fishery market at the south sea area during the period from October 1 to November 25, 1996. Specimens were examined for the infection status with larvae anisakid. Anisakid larvae were collected from viscera, muscle, omenturn and liver. Eight hundred four larval anisakids sorted from 93 specimens of A. myriaster ranged from 9.0 to 26.5mm in their body length. They were classified based on morphological and morphometric observations as follows Anisakis type 1 larvae (465 larvae, 57.8%: positive rate), Contracaecum type D(129, 16.0%), Contracaecum type A(90, 11.2%), Anisakis type 11(60, 7.5%), Phapidascaris(3, 0.4%) and unknown type(57, 7.1%).

• Parasites, Hosts and Diseases
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• 제50권4호
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• pp.295-299
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• 2012

The present study was performed to determine the infection status of anisakid larvae in marine fish collected from 3 sea areas of the Republic of Korea. Total 86 marine fish (8 species) collected from the East Sea (Goseong-gun, Gangwon-do), 171 fish (10 species) from the South Sea (Sacheon-si, Gyeongsangnam-do), and 92 fish (7 species) from the Yellow Sea (Incheon Metropolitan City) were examined by both naked eyes and artificial digestion method. Among the total of 349 fish examined, 213 (61.0%) were infected with 8 species of anisakid larvae, i.e., Anisakis simplex, 6 types of Contracaecum spp., and Raphidascaris sp., and the mean larval density was 13.8 per infected fish. Anisakid larvae were detected in 45 fish (52.3%) from the East Sea, 131 fish (76.6%) from the South Sea, and 37 fish (40.2%) from the Yellow Sea. The average numbers of larvae detected were 4.0, 16.6, and 15.9, respectively. Anisakis simplex larvae were detected in 149 fish (42.7%), and the mean larval density was 9.0 per infected fish. They were found in 26 fish (30.2%) collected from the East Sea, 96 fish (56.1%) from the South Sea, and 27 fish (29.3%) from the Yellow Sea. The average numbers of larvae detected were 2.9, 10.3, and 10.5, respectively. Conclusively, the present study suggests that the infection rate and density of anisakid larvae are more or less higher in the fish from the South Sea than those from the East Sea or the Yellow Sea.

• Parasites, Hosts and Diseases
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• 제57권6호
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• pp.699-704
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• 2019

Anisakiasis (anisakidosis) refers to a foodborne zoonosis caused by ingesting raw or undercooked marine fish or cephalopods infected with anisakid larvae. The present study was performed to investigate the prevalence of anisakid larvae in anchovies (Engraulis japonica) purchased from 2 local markets in Gyeongsangnam-do, the Republic of Korea (=Korea), during 2018-2019. Anchovies were transported to our laboratory and examined by pepsin-HCl artificial digestion technique followed by microscopic observations and molecular analyses. The overall prevalence of anisakid larvae was 19.5% (39/200), from which a total of 51 larvae (av. 1.3 larvae/infected anchovy) were recovered. Sequencing of the larvae targeting the ITS region, including ITS1, 5.8S rRNA, and ITS2 genes confirmed the species of larvae as Anisakis pegreffii (54.9%; 28/51), Hysterothylacium sinense (23.5%; 12/51), and Hysterothylacium aduncum (21.5%; 11/51). The results suggested that anchovies could be a potential source of human anisakiasis in Korea.

• 한국어병학회지
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• 제23권3호
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• pp.421-427
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• 2010

Anisakid nematodes third stage larvae were isolated from the muscles of masou salmon (Oncorhynchus masou). Fish were purchased from Jumunjin fishery market in Gangneung. Four Anisakid third stage larvae were isolated from 4 fish. Molecular identification of the isolated worms was conducted by PCR-RFLP analysis of ribosomal DNA internal transcribed spacer region and direct sequencing of mitochondrial DNA cox2 gene. As results, all the tested individual worms were identified as Anisakis simplex (sensu stricto). This is the first report of molecular detection of anisakid worms in salmonid fishes in Korea.

• 한국해양학회지:바다
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• 제1권1호
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• pp.55-57
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• 1996

1995년 11, 12월에 동해안 주문진 어시장에서 구입한 살오징어(Todarodes pacificus) 248개체중 34개체로부터 116개체의 아니사키스 유충이 채집되어 13.7% 의 유충 감염율을 나타내었다. 형태 및 크기를 바탕으로 유충의 종류 및 근육, 내장, 망 등 어체 부위별 감염상황을 조사한 결과 유충의 길이는 13.5-29.5 mm 이었으며 Anisakis type I이 68개체 (58.6%:회수율), Contracaecum type A 5개 체 (4.3%), Anisakis type II 17개체 (14.6%), Contracaecum type D 8개체 (7.0%) 및 불명 18개체 (15.5%)로 분류되었다.