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Molecular Phylogenetic Study of the Barbel Steed (Hemibarbus labeo) in Seomjin River of Korea (한국 섬진강산 누치(Hemibarbus labeo)의 분자 계통유전학적 연구)

  • Park, Kiyun;Lee, Wan-Ok;Kwak, Ihn-Sil
    • Korean Journal of Ecology and Environment
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    • v.52 no.3
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    • pp.221-230
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    • 2019
  • Barbel steed (Hemibarbus labeo) is a small freshwater fish species as semi-bottom dwellers distributed in eastern Asia. We carried out characterization of the cytochrome c oxidase subunit I (COI) gene from the mitochondrial DNA of H. labeo in the Sumjin River to identify the phylogenetic location of H. labeo in the genus Hemibarbus and Cyprinidae. Multiple alignment of the 577 bp COI sequence revealed high sequence homology (99~100%) between Seomjin River H. labeo. The nucleotide sequence similarity between H. labeo (HD1) and H. mylodon was 88.91% and that of H. longirostis was 88.81% among the three species found in Korea. In addition, the nucleotide sequence similarities of H. maculatus, H. meditus, H. umbrifer and H. barbus showed 98.97%, 97.20%, 96.87% and 98.85%, respectively. Phylogenetic analysis on seven species of the genus Hemibarbus showed that the H. labeo collected in this study formed two clades. One of which consisted of Hadong, Imsil, Kangjin. The other one formed a step with HD2, HD8 and HD9 of Hadong and the H. labeo reported in Busan, Asan and Seoul, Korea. Phylogenetic position of the H. labeo among Cyprinidae showed 0.143 for the evolutionary distance from Zacco platypus and 0.006 for the H. maculatus. In addition, the genetic position of the H. labeo among 28 species of Cyprinidae was found to be located in Group I, including Gobioninae fishes. The results of this study will provide key genetic information for the taxonomic comparison in Cyprinidae and study of model fish for pollution monitoring in freshwater environments.

Analysis of Road Surface Irregularity and Superelevation Using Mobile Mapping System (Mobile Mapping System을 이용한 도로 평탄성과 편경사 분석 연구)

  • KIM, Gi-Chang;YOON, Ha-Su;CHOI, Yun-Soo
    • Journal of the Korean Association of Geographic Information Studies
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    • v.22 no.3
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    • pp.155-166
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    • 2019
  • Road infrastructure has increased explosively due to economic development after industrialization and at present road infrastructure is being changed and increased by construction of new roads and maintenance and expansion of existing roads. Such road infrastructure should support safe driving. Road management plays an important role in safe driving. The purpose of this dissertation is to verify predictability of dangerous sections by analyzing road geometrical structure such as surface irregularity and superelevation for some sections in Central Inland Expressway by MMS and present ways of managing roads using MMS. Having analyzed surface irregularity of roads by using MMS, it was found that over 50 percent of all eight sections, targets of this study need betterments and for superelevation, over 50 percent of two sections goes against superelevation standard. Targets of this study are sections that accidents occurred frequently based on history of past accidents and predictability of dangerous sections can be verified through analysis of road geometrical structure using MMS. Using MMS data created by construction of high definition maps which are being undergone for all roads and methods proposed by this study will help investigate dangerous sections efficiently according to road environment. A result of MMS can be used for maintenance of road furniture.

Distribution and Molecular Phylogeny of the Toxic Benthic Dinoflagellate Ostreopsis sp. in the Coastal Waters off Jeju Island, Korea (춘계 제주 연안에서 유독 저서성 와편모류 Ostreopsis sp.의 분포와 분자계통학적 위치)

  • KIM, SUNJU;SEO, HYOJEONG
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.24 no.2
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    • pp.236-248
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    • 2019
  • We investigated occurrence and molecular phylogeny of the toxic epiphytic dinoflagellate Ostreopsis at seven sampling sites in the coastal waters off Jeju Island of Korea in April, 2017. During the sampling period, surface water temperature ranged from 15.7 to $18.3^{\circ}C$ and salinity was relatively constant, ranging from 33.4 to 34.9. Of a total of 13 macroalgal species collected from all sampling sites, Ostreopsis cells were observed from 8 macroalgal species and the highest cell abundance ($157.5cells\;g^{-1}$) was recorded on the red alga Grateloupia filicina at St. 6. LSU rDNA D8/D10 sequences of all Korean Ostreopsis strains isolated from the 4 sampling sites were 100% identical. Molecular phylogentic analyses (BI and ML) inferred from LSU rDNA alignment showed that the Korean Ostreopsis strains placed into the previously described the Ostreopsis sp. 1 clade, which contained strains isolated from the temperate coastal waters of Japan. The Korean Ostreopsis sp. 1 strain grew in a wide range of temperature ($10-30^{\circ}C$) and salinity (25-30), with its maximum growth rate of $0.49d^{-1}$ at $25^{\circ}C$ and salinity of 30, indicating that they can be tolerated in temperate areas.

High Thermal Conductivity h-BN/PVA Composite Films for High Power Electronic Packaging Substrate (고출력 전자 패키지 기판용 고열전도 h-BN/PVA 복합필름)

  • Lee, Seong Tae;Kim, Chi Heon;Kim, Hyo Tae
    • Journal of the Microelectronics and Packaging Society
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    • v.25 no.4
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    • pp.95-99
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    • 2018
  • High thermal conductivity films with electrically insulating properties have a great potential for the effective heat transfer as substrate and thermal interface materials in high density and high power electronic packages. There have been lots of studies to achieve high thermal conductivity composites using high thermal conductivity fillers such alumina, aluminum nitride, boron nitride, CNT and graphene, recently. Among them, hexagonal-boron nitride (h-BN) nano-sheet is a promising candidate for high thermal conductivity with electrically insulating filler material. This work presents an enhanced heat transfer properties of ceramic/polymer composite films using h-BN nano-sheets and PVA polymer resins. The h-BN nano-sheets were prepared by a mechanical exfoliation of h-BN flakes using organic media and subsequent ultrasonic treatment. High thermal conductivities over $2.8W/m{\cdot}K$ for transverse and $10W/m{\cdot}K$ for in-plane direction of the cast films were achieved for casted h-BN/PVA composite films. Further improvement of thermal conductivity up to $13.5W/m{\cdot}K$ at in-plane mode was achieved by applying uniaxial compression at the temperature above glass transition of PVA to enhance the alignment of the h-BN nano-sheets.

Association of polymorphisms in bone morphogenetic protein receptor-1B gene exon-9 with litter size in Dorset, Mongolian, and Small Tail Han ewes

  • Jia, Jianlei;Chen, Qian;Gui, Linsheng;Jin, Jipeng;Li, Yongyuan;Ru, Qiaohong;Hou, Shengzhen
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.7
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    • pp.949-955
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    • 2019
  • Objective: The present study was to investigate the association of polymorphisms in exon-9 of the bone morphogenetic protein receptor-1B (BMPR-1B) gene (C864T) with litter size in 240 Dorset, 232 Mongolian, and 124 Small Tail Han ewes. Methods: Blood samples were collected from 596 ewes and genomic DNA was extracted using the phenol: chloroform extraction method. The 304-bp amplified polymerase chain reaction product was analyzed for polymorphism by single-strand conformation polymorphism method. The genotypic frequency and allele frequency of BMPR-1B gene exon-9 were computed after sequence alignment. The ${\chi}^2$ independence test was used to analyze the association of genotypic frequency and litter size traits with in each ewe breed, where the phenotype was directly treated as category. Results: The results indicated two different banding patterns AA and AB for this fragment, with the most frequent genotype and allele of AA and A. Calculated Chi-square test for BMPR-1B gene exon-9 was found to be more than that of p value at the 5% level of significance, indicating that the population under study was in Hardy-Weinberg equilibrium for all ewes. The ${\chi}^2$ independence test analyses indicated litter size differences between genotypes was not the same for each breed. The 304-bp nucleotide sequence was subjected to BLAST analysis, and the C864T mutation significantly affected litter size in singletons, twins and multiples. The heterozygosity in exon-9 of BMPR-1B gene could increase litter size for all the studied ewes. Conclusion: Consequently, it appears that the polymorphism BMPR-1B gene exon-9 detected in this study may have potential use in marker assisted selection for litter size in Dorset, Mongolian, and Small Tail Han ewes.

Risk Factors of Allogenous Bone Graft Collapse in Two-Level Anterior Cervical Discectomy and Fusion

  • Woo, Joon-Bum;Son, Dong-Wuk;Lee, Su-Hun;Lee, Jun-Seok;Lee, Sang Weon;Song, Geun Sung
    • Journal of Korean Neurosurgical Society
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    • v.62 no.4
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    • pp.450-457
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    • 2019
  • Objective : Anterior cervical discectomy and fusion (ACDF) is commonly used surgical procedure for cervical degenerative disease. Among the various intervertebral spacers, the use of allografts is increasing due to its advantages such as no harvest site complications and low rate of subsidence. Although subsidence is a rare complication, graft collapse is often observed in the follow-up period. Graft collapse is defined as a significant graft height loss without subsidence, which can lead to clinical deterioration due to foraminal re-stenosis or segmental kyphosis. However, studies about the collapse of allografts are very limited. In this study, we evaluated risk factors associated with graft collapse. Methods : We retrospectively reviewed 33 patients who underwent two level ACDF with anterior plating using allogenous bone graft from January 2013 to June 2017. Various factors related to cervical sagittal alignment were measured preoperatively (PRE), postoperatively (POST), and last follow-up. The collapse was defined as the ratio of decrement from POST disc height to follow-up disc height. We also defined significant collapses as disc heights that were decreased by 30% or more after surgery. The intraoperative distraction was defined as the ratio of increment from PRE disc height to POST disc height. Results : The subsidence rate was 4.5% and graft collapse rate was 28.8%. The pseudarthrosis rate was 16.7% and there was no association between pseudarthrosis and graft collapse. Among the collapse-related risk factors, pre-operative segmental angle (p=0.047) and intra-operative distraction (p=0.003) were significantly related to allograft collapse. The cut-off value of intraoperative distraction ${\geq}37.3%$ was significantly associated with collapse (p=0.009; odds ratio, 4.622; 95% confidence interval, 1.470-14.531). The average time of events were as follows: collapse, $5.8{\pm}5.7months$; subsidence, $0.99{\pm}0.50months$; and instrument failure, $9.13{\pm}0.50months$. Conclusion : We experienced a higher frequency rate of collapse than subsidence in ACDF using an allograft. Of the various preoperative factors, intra-operative distraction was the most predictable factor of the allograft collapse. This was especially true when the intraoperative distraction was more than 37%, in which case the occurrence of graft collapse increased 4.6 times. We also found that instrument failure occurs only after the allograft collapse.

Analysis of the Risk Factors for Unfavorable Radiologic Outcomes after Fusion Surgery in Thoracolumbar Burst Fracture : What Amount of Postoperative Thoracolumbar Kyphosis Correction is Reasonable?

  • Seo, Dong Kwang;Kim, Chung Hwan;Jung, Sang Ku;Kim, Moon Kyu;Choi, Soo Jung;Park, Jin Hoon
    • Journal of Korean Neurosurgical Society
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    • v.62 no.1
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    • pp.96-105
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    • 2019
  • Objective : The aims in the management of thoracolumbar spinal fractures are not only to restore vertebral column stability, but also to obtain acceptable alignment of the thoracolumbar junction (T-L junction) to prevent complications. However, insufficient surgical correction of the thoracolumbar spine would be likely to cause late progression of abnormal kyphosis. Therefore, we identified the surgical factors that affected unfavorable radiologic outcomes of the thoracolumbar spine after surgery. Methods : This study was conducted in a single institution from January 2007 to December 2013. A total of 98 patients with unstable thoracolumbar spine fracture were included. In these patients, fixation was done through transpedicular screws with rods by three surgical patterns. We reviewed digital radiographs and analyzed the images preoperatively and postoperatively during follow-up visits to compare the change of the thoracolumbar Cobb angle with radiologic parameters and clinical outcomes. The unfavorable radiologic group was defined as the patients who were measured as having greater than 20 degrees of thoracolumbar Cobb angle on the last follow-up, or who underwent kyphotic progression of thoracolumbar Cobb angle greater than 10 degrees from the immediate postoperative state to final follow-up, or who had overt instrument failure with/without additional surgery. We assessed the risk factors that affected the unfavorable radiologic outcomes. Results : We had 43 patients with unfavorable radiologic outcomes, including 35 abnormal thoracolumbar alignments and 14 instrumental failures with/without additional surgery. The multivariate logistic regression test showed that immediate postoperative T-L junction Cobb angle less than 10.5 degrees was a statistically significant risk factor, as well as the presence of osteoporosis (p=0.017 and 0.049, respectively). Conclusion : Insufficient correction of thoracolumbar kyphosis was considered to be a major factor of an unfavorable radiological outcome. The spinal surgeon should consider that having a T-L junction Cobb angle larger than 10.5 degrees immediately after surgery could result in an unfavorable radiological outcome, which is related to a poor clinical outcome.

Analysis of Treatment Period on the Intraoral Removable Appliance Utilizing Vertical Facial Growth on Class III Malocclusion (얼굴의 수직성장을 이용하여 III급 부정교합을 치료하는 구강내 가철식 장치의 치료기간분석)

  • Song, Jihyeo;Kim, Seong-Oh;Song, Je Seon;Lee, Jaeho;Choi, Hyung-jun
    • Journal of the korean academy of Pediatric Dentistry
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    • v.46 no.2
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    • pp.173-182
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    • 2019
  • Vertical facial growth triggers the rotation of mandible to move the chin point to the downward and backward direction, which showed remarkably effective result making the less prominent chin. Recently, the intraoral removable appliance utilizing class III elastic demonstrated the vertical growth trigger mechanism. The treatment change was very fast and wearing was quite easy, compared to extraoral appliances. The purpose of this study was to verify the duration of the treatment on class III malocclusion using intraoral removable appliances, which designed to accelerate vertical facial growth. 56 patients were selected with the complaint of the protruded mandible and class III malocclusion (overjet : -3 - 0 mm, overbite : 0 - 4 mm). Information like; age at start, duration of the treatment events, type of the treatment, overjet, overbite etc. was collected and calculated. The average age of the patients delivering the initial brace was $8.75{\pm}1.10year$. Most of the anterior crossbite was resolved within 6 months. The total treatment period was $21.79{\pm}10.73months$ with the additional procedures like the alignment of anterior teeth and torque control using additional removable and fixed orthodontic appliances. The correlation study showed that patient's cooperation (p = 0.000) and the use of fixed appliance (p = 0.032) were significantly influenced on treatment duration.

The Effect of Transformational Leadership Characteristics on the Innovative Behavior of Follower -Focusing on the mediating effect of followership characteristics- (변혁적 리더십의 특성이 조직 구성원의 혁신행동에 미치는 영향 -팔로워십 특성의 매개효과를 중심으로-)

  • Song, Chang-Soo;Dong, Haklim
    • Journal of Digital Convergence
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    • v.19 no.4
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    • pp.119-131
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    • 2021
  • The purpose of this study is to confirm the mediating effect of followership characteristics when the components of transformational leadership influences the innovative behavior of follower. For this study, response data of nationwide 551 workers were finally analyzed using SPSS 24 and Process Macro 3.5. The research results are as follows. All of the components of transformational leadership had a positive(+) effect on innovative behavior. In this process, team spirit, the characteristic of followership, was confirmed to have a mediating effect in all relationships. Perception of goal alignment and active orientation were found to have mediating effects in some types of combination. On the other hand, critical thinking had no mediating effect. This study has academic significance that variables with complex characteristics were divided into sub-characteristics for analyzing. In practice, it is suggested that there is an effective combination type for each characteristic of the variables in promoting the innovative behavior of follower. Based on the research results, the limitations of the research and future research tasks were presented.

An Exploratory Study on Sales and Operations Planning as SCM Supporting Tool (공급망 관리 지원도구로서의 S&OP 운영에 관한 탐색적 연구)

  • Park, Seong Taek;Kim, Tae Ung;Kim, Mi Ryang
    • Journal of Digital Convergence
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    • v.19 no.2
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    • pp.93-103
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    • 2021
  • S&OP(Sales and Operations Planning) is an ongoing process of periodic planning, reviewing, and evaluation through the involvement of all key stakeholders. Within this process, performance is regularly reviewed and early warning signals are generated, so that the company can react quickly to changing market and operational environment. This paper presents a framework for effective S&OP for fair alignment, accountability, teamwork, visibility, and risk management. This framework focuses on supply chain information governance, level of information sharing through S&OP, role of S&OP as coordination mechanism, APS effectivesness as a planning tool and SCM performance. In addition, a brief case study on the operating characteristics of S&OP at three Korean firms is presented. Implications of the study finding are also provided. It will also make companies that are considering the introduction of S&OP aware of the importance of S&OP, which will provide practical guidelines for the introduction of S&OP.