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http://dx.doi.org/10.11614/KSL.2019.52.3.221

Molecular Phylogenetic Study of the Barbel Steed (Hemibarbus labeo) in Seomjin River of Korea  

Park, Kiyun (Fisheries Science Institute, Chonnam National University)
Lee, Wan-Ok (Fisheries Science Institute, Chonnam National University)
Kwak, Ihn-Sil (Fisheries Science Institute, Chonnam National University)
Publication Information
Korean Journal of Ecology and Environment / v.52, no.3, 2019 , pp. 221-230 More about this Journal
Abstract
Barbel steed (Hemibarbus labeo) is a small freshwater fish species as semi-bottom dwellers distributed in eastern Asia. We carried out characterization of the cytochrome c oxidase subunit I (COI) gene from the mitochondrial DNA of H. labeo in the Sumjin River to identify the phylogenetic location of H. labeo in the genus Hemibarbus and Cyprinidae. Multiple alignment of the 577 bp COI sequence revealed high sequence homology (99~100%) between Seomjin River H. labeo. The nucleotide sequence similarity between H. labeo (HD1) and H. mylodon was 88.91% and that of H. longirostis was 88.81% among the three species found in Korea. In addition, the nucleotide sequence similarities of H. maculatus, H. meditus, H. umbrifer and H. barbus showed 98.97%, 97.20%, 96.87% and 98.85%, respectively. Phylogenetic analysis on seven species of the genus Hemibarbus showed that the H. labeo collected in this study formed two clades. One of which consisted of Hadong, Imsil, Kangjin. The other one formed a step with HD2, HD8 and HD9 of Hadong and the H. labeo reported in Busan, Asan and Seoul, Korea. Phylogenetic position of the H. labeo among Cyprinidae showed 0.143 for the evolutionary distance from Zacco platypus and 0.006 for the H. maculatus. In addition, the genetic position of the H. labeo among 28 species of Cyprinidae was found to be located in Group I, including Gobioninae fishes. The results of this study will provide key genetic information for the taxonomic comparison in Cyprinidae and study of model fish for pollution monitoring in freshwater environments.
Keywords
Hemibarbus labeo; cytochrome c oxidase subunit I (COI); phylogenetic analysis; Cyprinidae; freshwater environment;
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