Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Association of polymorphisms in bone morphogenetic protein receptor-1B gene exon-9 with litter size in Dorset, Mongolian, and Small Tail Han ewes

  • Jia, Jianlei (Key of laboratory of Plateau Ecology and Agriculture, Qinghai University) ;
  • Chen, Qian (Department of Animal Science, College of agriculture and Animal Husbandry, Qinghai University) ;
  • Gui, Linsheng (Key of laboratory of Plateau Ecology and Agriculture, Qinghai University) ;
  • Jin, Jipeng (Department of Animal Science, College of Animal Science and Technology, Gansu Agricultural University) ;
  • Li, Yongyuan (Animal Husbandry and Veterinary Station) ;
  • Ru, Qiaohong (Animal Husbandry and Veterinary Station) ;
  • Hou, Shengzhen (Department of Animal Science, College of agriculture and Animal Husbandry, Qinghai University)
  • Received : 2018.07.20
  • Accepted : 2019.01.16
  • Published : 2019.07.01
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: The present study was to investigate the association of polymorphisms in exon-9 of the bone morphogenetic protein receptor-1B (BMPR-1B) gene (C864T) with litter size in 240 Dorset, 232 Mongolian, and 124 Small Tail Han ewes. Methods: Blood samples were collected from 596 ewes and genomic DNA was extracted using the phenol: chloroform extraction method. The 304-bp amplified polymerase chain reaction product was analyzed for polymorphism by single-strand conformation polymorphism method. The genotypic frequency and allele frequency of BMPR-1B gene exon-9 were computed after sequence alignment. The ${\chi}^2$ independence test was used to analyze the association of genotypic frequency and litter size traits with in each ewe breed, where the phenotype was directly treated as category. Results: The results indicated two different banding patterns AA and AB for this fragment, with the most frequent genotype and allele of AA and A. Calculated Chi-square test for BMPR-1B gene exon-9 was found to be more than that of p value at the 5% level of significance, indicating that the population under study was in Hardy-Weinberg equilibrium for all ewes. The ${\chi}^2$ independence test analyses indicated litter size differences between genotypes was not the same for each breed. The 304-bp nucleotide sequence was subjected to BLAST analysis, and the C864T mutation significantly affected litter size in singletons, twins and multiples. The heterozygosity in exon-9 of BMPR-1B gene could increase litter size for all the studied ewes. Conclusion: Consequently, it appears that the polymorphism BMPR-1B gene exon-9 detected in this study may have potential use in marker assisted selection for litter size in Dorset, Mongolian, and Small Tail Han ewes.

Keywords

References

  1. Ahlawat S, Sharma R, Maitra A, Tantia MS. Current status of molecular genetics research of goat fecundity. Small Rumin Res 2015;125:34-42. https://doi.org/10.1016/j.smallrumres.2015.01.027
  2. Davis GH. Fecundity genes in sheep. Anim Reprod Sci 2004;82-83:247-53. https://doi.org/10.1016/j.anireprosci.2004.04.001
  3. Zhang XM, Li WR, Wu YS, et al. Disruption of the sheep BMPR-IB gene by CRISPR/Cas9 in vitro-produced embryos. Theriogenology 2017;91:163-72. https://doi.org/10.1016/j.theriogenology.2016.10.025
  4. Rout M, Panigrahi S, Pradhan S, Routray A, Swain B. Genetic basis of fecundity in sheep. J Pharm Innov 2018;7:314-6.
  5. Polley S, De S, Batabyal S, et al. Polymorphism of fecundity genes (BMPR1B, BMP15 and GDF9) in the Indian prolific Black Bengal goat. Small Rumin Res 2009;85:122-9. https://doi.org/10.1016/j.smallrumres.2009.08.004
  6. Mahdavi M, Nanekarani S, Hosseini SD, et al. Mutation in BMPR-IB gene is associated with litter size in Iranian Kalehkoohi sheep. Anim Reprod Sci 2014;147(3-4):93-98. https://doi.org/10.1016/j.anireprosci.2014.04.003.
  7. Maskur M, Tapaul R, Kasip L. Genetic polymorphism of bone morphogenetic protein receptor 1B (BMPR-1B) gene and its association with litter size in Indonesian fat-tailed sheep. Afr J Biotechnol 2016;15:1315-9. https://doi.org/10.5897/AJB2015.15093
  8. Abdoli R, Zamani P, Mirhoseini SZ, et al. A review on prolifcacy genes in sheep. Reprod Domest Anim 2016;51:631-7. https://doi.org/10.1111/rda.12733
  9. Chu MX, Jia LH, Zhang YJ, et al. Polymorphisms of coding region of BMPR-IB gene and their relationship with litter size in sheep. Mol Biol Rep 2011;38:4071-6. https://doi.org/10.1007/s11033-010-0526-z
  10. Sharma L, Kuralkar SV, Ali S, Bankar PS, Kuralkar P. Polymorphism study of BMPR-1B and BMP-15 genes in Berari goats. Indian J Small Rumin 2016;22:230-3. https://doi.org/10.5958/0973-9718.2016.00062.3
  11. Sudhakar A, Rajendran R, Rahumathulla PS. Detection of booroola (FecB) mutation in Indian sheep-Nilagiri. Small Rumin Res 2013;113:55-7. https://doi.org/10.1016/j.smallrumres.2013.02.012
  12. Mishra AK, Arora AL, Kumar S, Prince LLL. Studies on effect of Booroola (FecB) genotype on lifetime ewes' productivity efficiency, litter size and number of weaned lambs in Garole X Malpura sheep. Anim Reprod Sci 2009;113:293-8. https://doi.org/10.1016/j.anireprosci.2008.06.002
  13. Yao YL, Reheman A, Xu YF, Li Q. MiR-125b contributes to ovarian granulosa cell apoptosis through targeting BMPR1B, a major gene for sheep prolificacy. Reprod Sci 2018;26:295-305. https://doi.org/10.1177%2F1933719118770544 https://doi.org/10.1177/1933719118770544
  14. Souza CJH, Baird DT. The booroola (FecB) mutation is associated with smaller adrenal glands in young adult ewes. Reprod Biomed Online 2004;8:414-8. https://doi.org/10.1016/S1472-6483(10)60925-X
  15. Dash S, Maity A, Bisoi PC, et al. Coexistence of polymorphism in fecundity genes BMPR1B and GDF9 of Indian Kendrapada sheep. Explor Anim Med Res 2017;7:33-8.
  16. Vacca GM, Dhaouadi A, Rekik M, Carcangiua V, Pazzolaa M, Dettori ML. Prolificacy genotypes at BMPR 1B, BMP15 and GDF9 genes in North African sheep breeds. Small Rumin Res 2010;88:67-71. https://doi.org/10.1016/j.smallrumres.2009.11.005
  17. Ding Q, Zhang LP, Jia JL, et al. Prokaryotic expression of FecB gene and generation of its polyclonal antibody. Acta Agric Boreali-Sinica 2015;30:31-6.
  18. Wang J, Zhang LP, Ma YT, et al. Polymorphism of BMPR-1B gene exon-7 in five populations. Chinese Agric Sci 2013;29:46-51.
  19. Li WW, Zhang LP, Wu JP, et al. Study on correlation between single nucleotide polymorphism of GH gene exon-4 and meat performance in Gannan Tibetan sheep. J Gansu Agric Univ 2013;48:10-4.
  20. Shokrollahi B, Morammazi S. Polymorphism of GDF9 and BMPR1B genes and their association with litter size in Markhoz goats. Reprod Domest Anim 2018;53:971-8. https://doi.org/10.1111/rda.13196
  21. Chu MX, Di R, Ye SC, et al. Establishment of molecular detection methods for high prolificacy major gene FecB in sheep and its application. J Agric Biotechnol 2009;17:52-8. https://doi.org/10.3969/j.issn.1674-7968.2009.01.010
  22. Maskur M, Tapaul R, Kasip L. Genetic polymorphism of bone morphogenetic protein receptor 1B (BMPR-1B) gene and its association with litter size in indonesian fat-tailed sheep. Afr J Biotechnol 2016;15:1315-9. https://doi.org/10.5897/AJB2015.15093
  23. Yang YY, Shao K, Lai DA, et al. Study on the BMP15 gene as candidate genes for fecundity in mongolian sheep doublelambs group. Acta Agriculturae Boreali-Sinica 2010;25:44-9. https://doi.org/10.7668/hbnxb.2010.01.009
  24. He XL, Liu YB, Wang F. Mongolian sheep $FSH{\beta}$ gene cDNA cloning and sequence analysis. Heilongjiang Anim Sci Vet Med 2010;39:39-41.
  25. Abdoli R, Zamani P, Deljou A, Rezvan H. Association of BMPR-1B and GDF9 genes polymorphisms and secondary protein structure changes with reproduction traits in mehraban ewes. Gene 2013;524:296-303. https://doi.org/10.1016/j.gene.2013.03.133
  26. Ghaderi A, Nasiri MB, Mirzadeh KH, Fayazi J, Sadr AS. Identification of the GDF9 mutation in two sheep breeds by using polymerase chain reaction-restriction fragment length polymorphism technique. Afr J Biotechnol 2010;35:8020-2.
  27. Roy J, Polley S, De S, et al. Polymorphism of fecundity genes (FecB, FecX, and FecG) in the indian bonpala sheep. Anim Biotechnol 2011;22:151-62. https://doi.org/10.1080/10495398.2011.589239
  28. Moradband F, Rahimi G, Gholizadeh M. Association of polymorphisms in fecundity genes of GDF9, BMP15 and BMP15-1B with litter size in Iranian Baluchi sheep. Asian-Australas J Anim Sci 2011;24:1179-83. https://doi.org/10.5713/ajas.2011.10453
  29. Jia JL, Zhang LP, Ding Q, et al. Correlation between polymorphism of BMPR-1B gene exon-7 and lambing performance of sheep. J Northwest A and F Univ 2016;44:7-13.
  30. Gui L, Hao R, Zhang Y, Zhao X, Zan L. Haplotype distribution in the class I sirtuin genes and their associations with ultrasound carcass traits in Qinchuan cattle (Bos taurus). Mol Cell Probes 2015;29:167-71. https://doi.org/10.1016/j.mcp.2015.03.007
  31. Zhang X, Zhou L, Fu G, et al. The identification of an ESCC susceptibility SNP rs920778 that regulates the expression of lncRNA HOTAIR via a novel intronic enhancer. Carcinogenesis 2014;35:2062-7. https://doi.org/10.1093/carcin/bgu103
  32. Pan W, Liu L, Wei J, et al. A functional lncRNA HOTAIR genetic variant contributes to gastric cancer susceptibility. Mol Carcinog 2016;55:90-6. https://doi.org/10.1002/mc.22261
  33. Dechamethakun S, Muramatsu M. Long noncoding RNA variations in cardiometabolic diseases. J Hum Genet 2017;62:7-104. https://doi.org/10.1038/jhg.2016.70

Cited by

  1. Association between novel variants in BMPR1B gene and litter size in Mongolia and Ujimqin sheep breeds vol.56, pp.12, 2021, https://doi.org/10.1111/rda.14020