• The Korean Journal of Physiology and Pharmacology
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• 제25권2호
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• pp.147-157
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• 2021

Coronary microembolization (CME) is associated with cardiomyocyte apoptosis and cardiac dysfunction. Puerarin confers protection against multiple cardiovascular diseases, but its effects and specific mechanisms on CME are not fully known. Hence, our study investigated whether puerarin pretreatment could alleviate cardiomyocyte apoptosis and improve cardiac function following CME. The molecular mechanism associated was also explored. A total of 48 Sprague-Dawley rats were randomly divided into CME, CME + Puerarin (CME + Pue), sham, and sham + Puerarin (sham + Pue) groups (with 12 rats per group). A CME model was established in CME and CME + Pue groups by injecting 42 ㎛ microspheres into the left ventricle of rats. Rats in the CME + Pue and sham + Pue groups were intraperitoneally injected with puerarin at 120 mg/kg daily for 7 days before operation. Cardiac function, myocardial histopathology, and cardiomyocyte apoptosis index were determined via cardiac ultrasound, hematoxylin-eosin (H&E) and hematoxylin-basic fuchsin-picric acid (HBFP) stainings, and TdT-mediated dUTP nick-end labeling (TUNEL) staining, respectively. Western blotting was used to measure protein expression related to the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/glycogen synthase kinase-3β (GSK-3β) pathway. We found that, puerarin significantly ameliorated cardiac dysfunction after CME, attenuated myocardial infarct size, and reduced myocardial apoptotic index. Besides, puerarin inhibited cardiomyocyte apoptosis, as revealed by decreased Bax and cleaved caspase-3, and up-regulated Bcl-2 and PI3K/Akt/GSK-3β pathway related proteins. Collectively, puerarin can inhibit cardiomyocyte apoptosis and thus attenuate myocardial injury caused by CME. Mechanistically, these effects may be achieved through activation of the PI3K/Akt/GSK-3β pathway.

• 산업융합연구
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• 제18권6호
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• pp.61-73
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• 2020

본 연구는 고령친화마을 만들기를 위한 노인일자리 참여 노인의 갈등경험을 탐색하여 갈등해결을 유형화하는데 목적이 있다. 본 연구는 질적 연구방법인 근거이론을 적용하였으며, 자료수집을 위해 서울시 ◯◯구 ◯◯동에서 노인일자리 참여자 5명을 표본 추출하여 FGI를 실시하였다. 자료분석은 Strauss & Corbin(1998)의 패러다임 모형에 맞추어 분석하였다. 분석결과, 인과적 조건은 사회적, 경제적, 심리·신체적 참여동기로, 맥락적 조건은 경제적 어려움, 사회적 단절, 삶의 경험 차이가 갈등유발에 영향을 미쳤다. 중심현상은 '경험과 역할의 차이'와 '심리·사회관계 기술의 차이'에 의해 갈등이 시작되고, 중재적 조건은 갈등해소를 위한 의사소통을 통해 대인관계 활성화, 능력 활용을 통한 사회통합, 규칙적인 사회활동으로 나타났다. 상호작용 전략은 교육하기, 신뢰감 형성을 위한 상호지지, 성공적인 고령친화마을 프로그램 운영으로 노인일자리 갈등해소 전략으로 나타났다. 결과는 자신의 삶 찾기, 지역사회교류 활성화, 성취감 증가, 공동체 의식이 성장함으로써 노인일자리 갈등이 완화되는 것으로 나타났다. 결론적으로 노인일자리 갈등 해결 유형은 교육 해결형, 상호지지 해결형, 성공 경험 해결형으로 유형화되었다.

• The Korean Journal of Pain
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• 제34권1호
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• pp.58-65
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• 2021

Background: Supraspinal delivery of neurotensin (NTS), which may contribute to the effect of a systemically administered agonist, has been reported to be either pronociceptive or antinociceptive. Here, we evaluated the effects of systemically administered NTSR1 agonist in a rat model of neuropathic pain and elucidated the underlying supraspinal mechanism. Methods: Neuropathic pain was induced by L5 and L6 spinal nerve ligation in male Sprague-Dawley rats. The effects of intraperitoneally administered NTSR1 agonist PD 149163 was assessed using von Frey filaments. To examine the role of 5-HT neurotransmission, a serotonin (5-HT) receptor antagonist dihydroergocristine was pretreated intrathecally, and spinal microdialysis studies were performed to measure the change in extracellular level of 5-HT in response to PD 149163 administration. To investigate the supraspinal mechanism, NTSR1 antagonist 48692 was microinjected into the rostral ventromedial medulla (RVM) prior to systemic PD 149163. Additionally, the effect of intrathecal DHE on intra-RVM PD 149163 was assessed. Results: Intraperitoneally administered PD 149163 exhibited a dose-dependent attenuation of mechanical allodynia. This effect was partially reversed by intrathecal pretreatment with dihydroergocristine and was accompanied by an increased extracellular level of 5-HT in the spinal cord. The PD 149163-produced antinociception was also blocked by intra-RVM SB 48692. Direct injection of PD 149163 into the RVM mimicked the maximum effect of the same drug delivered intraperitoneally, which was reversed by intrathecal dihydroergocristine. Conclusions: These observations indicate that systemically administered NTSR1 agonist produces antinociception through the NTSR1 in the RVM, activating descending serotonergic projection to release 5-HT into the spinal dorsal horn.

• The Korean Journal of Pain
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• 제34권1호
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• pp.27-34
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• 2021

Background: Chemotherapy-induced peripheral neuropathy (CIPN) is a major reason for stopping or changing anticancer therapy. Among the proposed pathomechanisms underlying CIPN, proinflammatory processes have attracted increasing attention. Here we assessed the role of prostaglandin D2 (PGD2) signaling in cisplatin-induced neuropathic pain. Methods: CIPN was induced by intraperitoneal administration of cisplatin 2 mg/kg for 4 consecutive days using adult male Sprague-Dawley rats. PGD2 receptor DP1 and/or DP2 antagonists were administered intrathecally and the paw withdrawal thresholds were measured using von Frey filaments. Spinal expression of DP1, DP2, hematopoietic PGD synthase (H-PGDS), and lipocalin PGD synthase (L-PGDS) proteins were analyzed by western blotting. Results: The DP1 and DP2 antagonist AMG 853 and the selective DP2 antagonist CAY10471, but not the DP1 antagonist MK0524, significantly increased the paw withdrawal threshold compared to vehicle controls (P = 0.004 and P < 0.001, respectively). Western blotting analyses revealed comparable protein expression levels in DP1 and DP2 in the spinal cord. In the CIPN group the protein expression level of L-PGDS, but not of H-PGDS, was significantly increased compared to the control group (P < 0.001). Conclusions: The findings presented here indicate that enhanced PGD2 signaling, via upregulation of L-PGDS in the spinal cord, contributes to mechanical allodynia via DP2 receptors in a cisplatin-induced neuropathic pain model in rats, and that a blockade of DP2 receptor activation may present a novel therapeutic target for managing CIPN.

• 기술혁신연구
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• 제29권1호
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• pp.1-38
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• 2021

본 논문에서는 에너지 섹터의 혁신성 제고를 위한 리빙랩(Living Labs) 활용 전략 수립에 관한 탐색적 분석 연구를 수행하였다. 기존 연구문헌을 통해 이론적 배경이 되는 리빙랩의 개념, 필수 구성요소, 혁신성 특성과 에너지 섹터의 혁신 이슈 유형을 확인할 수 있었다. 이를 토대로 리빙랩 필수 구성요소의 맞춤형 전략 수립과 혁신성 제고와의 연관성, 에너지 섹터에서의 혁신 이슈에 대한 접근으로 리빙랩 활용 가능성에 대해 에너지 리빙랩 사례 연구(해외 8건, 국내 1건)을 통해 분석되었다. 연구 결과, 리빙랩 필수 구성요소의 맞춤형 전략 수립이 혁신성 제고에 동인으로 작용 될 수 있으며, 리빙랩이 에너지 섹터의 혁신 이슈(수요관리, 공급기술, 기술사업화 촉진 및 수용성, 기술정책)에 대한 접근 방법론으로 효과적으로 활용 될 수 있음을 확인되었다. 사례연구 결과 기반으로 혁신성 기여 관점에서 리빙랩 사례별 동인을 도출하고 에너지 혁신이슈별 리빙랩 활용 전략을 수립하였다. 본 연구는 에너지 섹터에서의 혁신이슈에 대한 접근으로 리빙랩 모델의 활용 전략과 그 가치에 대한 탐색적, 기술적 분석 연구로서 종래에 시도되지 않았던 리빙랩 활용 전략 프레임워크를 제공할 수 있고, 에너지 리빙랩 활성화와 네트워크 형성에도 기여될 수 있다는 점에서 학술적·실용적·정책적 함의가 있다고 볼 수 있다.

• 한국운동역학회지
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• 제32권1호
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• pp.17-23
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• 2022

Objective: The aim of this study was to investigate the effect Tiger-step walking on the movement of the lower extremities during walking. Method: Twenty healthy male adults who had no experience of musculoskeletal injuries on lower extremities in the last six months (age: 26.85 ± 3.28 yrs, height: 174.6 ± 3.72 cm, weight: 73.65 ± 7.48 kg) participated in this study. In this study, 7-segments whole-body model (pelvis, both side of thigh, shank and foot) was used and 29 reflective markers and cluster were attached to the body to identify the segments during the gait. A 3-dimensional motion analysis with 8 infrared cameras and 7 channeled EMG was performed to find the effect of tigerstep on uphill walking. To verify the tigerstep effect, a one-way ANOVA with a repeated measure was used and the statistical significance level was set at α=.05. Results: Firstly, Both Tiger-steps showed a significant increase in stance time and stride length compared with normal walking (p<.05), while both Tiger-steps shown significantly reduced cadence compared to normal walking (p<.05). Secondly, both Tiger-steps revealed significantly increased in hip and ankle joint range of motion compared with normal walking at all planes (p<.05). On the other hand, both Tiger-steps showed significantly increased knee joint range of motion compared with normal walking at the frontal and transverse planes (p<.05). Lastly, Gluteus maximus, biceps femoris, medial gastrocnemius, tibialis anterior of both tiger-step revealed significantly increased muscle activation compared with normal walking in gait cycle and stance phase (p<.05). On the other hand, in swing phase, the muscle activity of the vastus medialis, biceps femoris, tibialis anterior of both tiger-step significantly increased compared with those of normal walking (p <.05). Conclusion: As a result of this study, Tiger step revealed increased in 3d range of motion of lower extremity joints as well as the muscle activities associated with range of motion. These findings were evaluated as an increase in stride length, which is essential for efficient walking. Therefore, the finding of this study prove the effectiveness of the tiger step when walking uphill, and it is thought that it will help develop a more efficient tiger step in the future, which has not been scientifically proven.

• 공업화학
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• 제33권6호
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• pp.636-645
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• 2022

이 연구에서는 은행껍질기반 활성탄으로 음이온성 염료인 메틸오렌지(MO)의 흡착 특성을 조사하였다. 이를 위해 은행껍질과 대표적인 화학활성화제인 수산화칼륨(KOH)을 이용하여 서로 다른 기공 특성을 지닌 다공성 활성탄(GS-1, GS-2, GS-4)을 제조하였다. 제조한 활성탄의 구조적 특성값과 KOH 혼합비율과의 상관관계는 질소 흡/탈착등온선으로 조사하였다. 활성탄에 대한 MO 흡착 평형 실험은 서로 다른 pH (pH 3~11) 및 온도(298~318 K) 조건에서 실시하였으며 그 결과를 Langmuir, Freundlich, Sips 및 온도 의존 Sips식으로 살펴보았다. 그리고 Langmuir 무차원 분리계수값으로 제조한 활성탄의 MO 흡착처리공정의 타당성을 조사하였다. 흡착에너지분포함수(AED)로 비교 분석한 활성탄에 대한 MO의 불균일 흡착 특성은 온도와 활성탄의 구조적 특성과 밀접한 관련이 있었다. 서로 다른 온도에서 수행한 회분식 흡착 속도 실험 결과는 외부물질전달, 입자 내 확산 및 활성사이트의 흡착을 고려한 균일표면확산모델(HSDM)로 만족스럽게 설명할 수 있었다. 또한 표면확산계수값을 Arrhenius 플롯으로 나타내어 구한 활성화에너지와 흡착에너지분포 함수값과의 상관관계를 살펴보았다. 그리고 Biot 수를 이용하여 제조한 활성탄에 대한 MO의 흡착 공정 메커니즘을 평가하였다.

• 대한본초학회지
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• 제36권6호
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• pp.1-8
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• 2021

Objectives : Atractylodis rhizoma Alba has been traditionally used as a medicinal resource that is used for enhancing Qi (氣) in traditional medicine in Korea, China, and Japan. This study investigated the protective effects of Atractylodis rhizoma Alba extract (ARE) against trimethyltin (TMT), a neurotoxin that causes selective hippocampal injury, using both in vitro and in vivo models. Methods : We investigated the effects of ARE on TMT- (5mM) induced cytotoxicity in primary cultures of mouse hippocampal cells (7 days in vitro ) and on hippocampal injury in C57BL/6 mice injected with TMT (2.6 mg/kg). Results : We observed that ARE treatment (0 - 50 ㎍/mL) significantly reduced TMT-induced cytotoxicity in cultured hippocampal neurons in a dose-dependent manner, based on results of lactate dehydrogenase and 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide assays. Additionally, this study showed that orally administered ARE (5 mg/kg; between -6 and 0 days before TMT injection) significantly attenuated seizures in adult mice. Furthermore, quantitative analysis of allograft inflammatory factor-1 (Iba-1)- and glial fibrillary acidic protein (GFAP)- positive cells showed significantly reduced levels of Iba-1- and GFAP-positive cell bodies in the dentate gyrus of mice treated with ARE prior to TMT injection. These findings indicate the significant protective effects of ARE against the TMT-induced massive activation of microglia and astrocytes in the hippocampus. Conclusions : We conclude that ARE minimizes the detrimental effects of TMT-induced hippocampal neurotoxicity, both in vitro and in vivo . Our findings may serve as useful guidelines to support ARE administration as a promising pharmacotherapeutic approach to hippocampal degeneration.

• 중소기업연구
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• 제42권4호
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• pp.251-283
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• 2020

본 연구는 우리나라의 중소기업들에 대하여 스마트 팩토리 관련 기술의 수용 및 사용의 수준을 제고하기 위한 관련 정책의 수립에 도움을 제공하기 위한 것이다. 이를 위하여 통합기술수용모델(UTAUT)을 확장하여 기술 수용의도에 영향을 미칠 수 있는 추가적인 영향요인들을 선정하였으며, 이를 실증하는 것이다. 연구목적의 달성을 위하여 리커트 7점 척도로 구성된 설문지를 작성하였으며, 제조 관련 기업을 대상으로 설문조사를 시행하였다. 통계처리에 활용된 설문지는 총 136부이다. 가설검정 결과 성과기대와 사회적 영향은 수(사)용의도에 정(+)의 유의한 영향을 미치지만, 노력기대와 촉진조건은 유의한 영향을 미치지 않는 것으로 검정되었다. 확장요인으로서 네트워크 효과, 조직특성은 정(+)의 유의한 영향을 미치며, 혁신저항은 부(-)의 유의한 영향을 미치지만 인지된 위험은 유의한 영향을 미치지 않는 것으로 검정되었다. 기업의 규모가 클수록 인지된 위험과 혁신저항의 수준은 낮으며, 이를 제외한 수(사)용의도 영향요인과, 수(사)용의도, 수(사)용행동의 수준은 높은 것으로 검정되었다. 본 연구를 통하여 스마트 팩토리 관련 기술의 수(사)용에 긍정적, 부정적 영향을 미칠 수 있는 요인들을 규명함으로서 제고되어야 하는 요인과 감소시켜야 하는 요인을 제시하였으며, 비교적 기업의 규모가 큰 기업들부터 우선적으로 스마트 팩토리 관련 기술이 수용될 수 있도록 하여야 한다는 연구결과를 제시한다.

• 대한본초학회지
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• 제34권1호
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• pp.51-57
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• 2019

Objectives : Nypa fruticans Wurmb. (NF) have been used as a traditional medicine to treat inflammatory diseases in East-South Asia. However, it is largely undiscovered whether NF water extract could exhibit anti-inflammatory activities against tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$)-induced inflammatory responses on human keratinocytes, HaCaT cells. Therefore, this study was aimed to investigate the anti-inflammatory activity of NF water extract on TNF-${\alpha}$-induced inflammatory responses in HaCaT cells. Methods : To investigate the anti-inflammatory activites of NF water extract in HaCaT cells, the inflammatory model of HaCaT cells was established under a suitable concentration (10 ng/ml) of human TNF-${\alpha}$ (hTNF-${\alpha}$). HaCaT keratinocyte cells were pre-treated with NF water extract for 1 h, and then stimulated with hTNF-${\alpha}$. Then, the cells were harvested to measure the inflammatory mediators such as inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and prostaglandin $E_2$ ($PGE_2$), and pro-inflammatory cytokine including TNF-${\alpha}$ and interleukin (IL)-6. In addition, we examined the inhibitory mechanisms of NF, mitogen activated protein kinases (MAPKs) and inhibitory kappa B alpha ($I{\kappa}-B{\alpha}$) Results : The treatment of NF inhibited the hTNF-${\alpha}$-induced elevation of iNOS, COX-2, and $PGE_2$ in HaCaT cells. In addition, NF treatment inhibited the hTNF-${\alpha}$-induced elevation of TNF-${\alpha}$ and IL-6. Furthermore, NF treatment inhibited the activation of MAPKs but not degradation of $I{\kappa}-B{\alpha}$. Conclusions : Taken together, our result suggest that treatment of NF could inhibit the hTNF-${\alpha}$-induced inflammatory responses via deactivation of MAPKs in HaCaT cells. This study could suggest that NF could be a beneficial agent to prevent skin damage or inflammation.