The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Protective effects of Atractylodis Rhizoma Alba Extract on seizures mice model

뇌전증 동물 모델에 대한 백출 추출물의 보호 효과

  • Kang, Sohi (College of Veterinary Medicine (BK21 Project Team), Chonnam National University) ;
  • Lee, Su Eun (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Ayeong (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Seo, Yun-Soo (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Moon, Changjong (College of Veterinary Medicine (BK21 Project Team), Chonnam National University) ;
  • Kim, Sung Ho (College of Veterinary Medicine (BK21 Project Team), Chonnam National University) ;
  • Lee, Jihye (College of Korean Medicine, Semyung University) ;
  • Kim, Joong Sun (College of Veterinary Medicine (BK21 Project Team), Chonnam National University)
  • 강소희 (전남대학교 수의과대학) ;
  • 이수은 (한국한의학연구원 한약자원연구센터) ;
  • 이아영 (한국한의학연구원 한약자원연구센터) ;
  • 서윤수 (한국한의학연구원 한약자원연구센터) ;
  • 문창종 (전남대학교 수의과대학) ;
  • 김성호 (전남대학교 수의과대학) ;
  • 이지혜 (세명대학교 한의과대학) ;
  • 김중선 (전남대학교 수의과대학)
  • Received : 2021.08.11
  • Accepted : 2021.11.25
  • Published : 2021.11.30
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Abstract

Objectives : Atractylodis rhizoma Alba has been traditionally used as a medicinal resource that is used for enhancing Qi (氣) in traditional medicine in Korea, China, and Japan. This study investigated the protective effects of Atractylodis rhizoma Alba extract (ARE) against trimethyltin (TMT), a neurotoxin that causes selective hippocampal injury, using both in vitro and in vivo models. Methods : We investigated the effects of ARE on TMT- (5mM) induced cytotoxicity in primary cultures of mouse hippocampal cells (7 days in vitro ) and on hippocampal injury in C57BL/6 mice injected with TMT (2.6 mg/kg). Results : We observed that ARE treatment (0 - 50 ㎍/mL) significantly reduced TMT-induced cytotoxicity in cultured hippocampal neurons in a dose-dependent manner, based on results of lactate dehydrogenase and 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide assays. Additionally, this study showed that orally administered ARE (5 mg/kg; between -6 and 0 days before TMT injection) significantly attenuated seizures in adult mice. Furthermore, quantitative analysis of allograft inflammatory factor-1 (Iba-1)- and glial fibrillary acidic protein (GFAP)- positive cells showed significantly reduced levels of Iba-1- and GFAP-positive cell bodies in the dentate gyrus of mice treated with ARE prior to TMT injection. These findings indicate the significant protective effects of ARE against the TMT-induced massive activation of microglia and astrocytes in the hippocampus. Conclusions : We conclude that ARE minimizes the detrimental effects of TMT-induced hippocampal neurotoxicity, both in vitro and in vivo . Our findings may serve as useful guidelines to support ARE administration as a promising pharmacotherapeutic approach to hippocampal degeneration.

Keywords

Acknowledgement

본 연구에 사용된 시료는 한국한의학연구원 한약표준표본관에 제공받았습니다. 시료를 제공해준 한국한의학연구원 한약표준표본관에 감사드립니다.

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