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http://dx.doi.org/10.6116/kjh.2021.36.6.1.

Protective effects of Atractylodis Rhizoma Alba Extract on seizures mice model  

Kang, Sohi (College of Veterinary Medicine (BK21 Project Team), Chonnam National University)
Lee, Su Eun (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine)
Lee, Ayeong (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine)
Seo, Yun-Soo (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine)
Moon, Changjong (College of Veterinary Medicine (BK21 Project Team), Chonnam National University)
Kim, Sung Ho (College of Veterinary Medicine (BK21 Project Team), Chonnam National University)
Lee, Jihye (College of Korean Medicine, Semyung University)
Kim, Joong Sun (College of Veterinary Medicine (BK21 Project Team), Chonnam National University)
Publication Information
The Korea Journal of Herbology / v.36, no.6, 2021 , pp. 1-8 More about this Journal
Abstract
Objectives : Atractylodis rhizoma Alba has been traditionally used as a medicinal resource that is used for enhancing Qi (氣) in traditional medicine in Korea, China, and Japan. This study investigated the protective effects of Atractylodis rhizoma Alba extract (ARE) against trimethyltin (TMT), a neurotoxin that causes selective hippocampal injury, using both in vitro and in vivo models. Methods : We investigated the effects of ARE on TMT- (5mM) induced cytotoxicity in primary cultures of mouse hippocampal cells (7 days in vitro ) and on hippocampal injury in C57BL/6 mice injected with TMT (2.6 mg/kg). Results : We observed that ARE treatment (0 - 50 ㎍/mL) significantly reduced TMT-induced cytotoxicity in cultured hippocampal neurons in a dose-dependent manner, based on results of lactate dehydrogenase and 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide assays. Additionally, this study showed that orally administered ARE (5 mg/kg; between -6 and 0 days before TMT injection) significantly attenuated seizures in adult mice. Furthermore, quantitative analysis of allograft inflammatory factor-1 (Iba-1)- and glial fibrillary acidic protein (GFAP)- positive cells showed significantly reduced levels of Iba-1- and GFAP-positive cell bodies in the dentate gyrus of mice treated with ARE prior to TMT injection. These findings indicate the significant protective effects of ARE against the TMT-induced massive activation of microglia and astrocytes in the hippocampus. Conclusions : We conclude that ARE minimizes the detrimental effects of TMT-induced hippocampal neurotoxicity, both in vitro and in vivo . Our findings may serve as useful guidelines to support ARE administration as a promising pharmacotherapeutic approach to hippocampal degeneration.
Keywords
Atractylodis Rhizoma Alba; trimethyltin; neuronal degeneration; hippocampus; seizure;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Lee SY, Jung KY, Lee IK, Do Yi S, Cho YW, Kim DW, et al. Prevalence of treated epilepsy in Korea based on national health insurance data. Journal of Korean medical science. 2012;27(3):285-90.   DOI
2 Ishida N, Akaike M, Tsutsumi S, Kanai H, Masui A, Sadamatsu M, et al. Trimethyltin syndrome as a hippocampal degeneration model: temporal changes and neurochemical features of seizure susceptibility and learning impairment. Neuroscience. 1997;81(4): 1183-91.   DOI
3 Nishimura T, Imai H, Minabe Y, Sawa A, Kato N. Beneficial effects of FK506 for experimental temporal lobe epilepsy. Neuroscience research. 2006;56(4):386-90.   DOI
4 Geloso MC, Corvino V, Michetti F. Trimethyltininduced hippocampal degeneration as a tool to investigate neurodegenerative processes. Neurochemistry international. 2011;58(7):729-38.   DOI
5 Park ST, Lee MS, Jeon BH, Park KI, Oh JM. Effect of Atractylodis Rhizoma alba on osteoclast formation. Journal of Physiology & Pathology in Korean Medicine. 2011;25(1):109-14.
6 Han YK, Park YK. Effect of Atractylodis Rhizoma Alba water extract on streptozotocin-induced diabetes in rats. The Korea Journal of Herbology. 2011;26(4):23-30.   DOI
7 Seo YS, Ang MJ, Moon BC, Kim HS, Choi G, Lim HS, et al. Protective Effects of Scolopendra Water Extract on Trimethyltin-Induced Hippocampal Neurodegeneration and Seizures in Mice. Brain sciences. 2019;9(12):369.   DOI
8 Lee S, Seo YH, Song JH, Kim WJ, Lee JH, Moon BC, et al. Neuroprotective Effect of Protaetia brevitarsis seulensis' Water Extract on Trimethyltin-Induced Seizures and Hippocampal Neurodegeneration. International Journal of Molecular Sciences. 2021;22(2):679.   DOI
9 Gao Q, Ji ZH, Yang Y, Cheng R, Yu XY. Neuroprotective effect of Rhizoma Atractylodis macrocephalae against excitotoxicity-induced apoptosis in cultured cerebral cortical neurons. Phytotherapy Research. 2012;26(4):557-61.   DOI
10 Li X, Lin J, Han W, Mai W, Wang L, Li Q, et al. Antioxidant ability and mechanism of rhizoma Atractylodes macrocephala. Molecules. 2012;17(11): 13457-72.   DOI
11 Bailly C. Atractylenolides, essential components of Atractylodes-based traditional herbal medicines: antioxidant, anti-inflammatory and anticancer properties. European journal of pharmacology. 2020:173735.
12 Perretta G, Righi FR, Gozzo S. Neuropathological and behavioral toxicology of trimethyltin exposure. ANNALI-ISTITUTO SUPERIORE DI SANITA. 1993;29:167-74.
13 Jang S, Choi S, Park C, Ahn M, Shin T, Kim S. Inducible nitric oxide synthase is involved in neuronal death induced by trimethyltin in the rat hippocampus. Korean Journal of Veterinary Research. 2011;51(3):185-91.   DOI
14 Park MH, Kim CJ, Lee JY, Keum CY, Kim IS, Jin CH, et al. Anti-Arthritic Effect of Radiation Mutant Perilla frutescens var. crispa and Atractylodes macrophala koidz. Journal of the Korean Applied Science and Technology. 2020;37(1):102-13.
15 JE Oh, SH Bae. The Effect of Anxiety and Depression on Sleep Quality among Adult Epilepsy Patients. Health & Nursing. 2018;30(2):21-32.   DOI
16 Bin CH, Song CH. Ameliorating Effects of Atractylodes macrocephala Koidzumi on TNF-α- induced 3T3-L1 Adipocyte Dysfunction. Korean Journal of Acupuncture. 2015;32(3):116-23.   DOI
17 Barker-Haliski M, Sills GJ, White HS. What are the arguments for and against rational therapy for epilepsy? Issues in clinical epileptology: a view from the bench. 2014:295-308.
18 Korean Epilepsy Society. Epilepsy [Internet]. Korea: Korean Epilepsy Society; 2018 [cited 2018 Jun 15] Available from : http://www.kes.or.kr/sub04/sub01.html.
19 Duncan JS, Sander JW, Sisodiya SM, Walker MC. Adult epilepsy. The Lancet. 2006;367(9516):1087-100.   DOI
20 Falco-Walter JJ, Scheffer IE, Fisher RS. The new definition and classification of seizures and epilepsy. Epilepsy research. 2018;139:73-9.   DOI
21 Jung KY. Epilepsy: More than Seizures. Epilia: Epilepsy and Community. 2020;2(2):31-2.   DOI
22 TALLIS R, HALL G, CRAIG I, DEAN A. How common are epileptic seizures in old age? Age and ageing. 1991;20(6):442-8.   DOI
23 Tsunashima K, Sadamatsu M, Takahashi Y, Kato N, Sperk G. Trimethyltin intoxication induces marked changes in neuropeptide expression in the rat hippocampus. Synapse. 1998;29(4):333-42.   DOI
24 Jung J, Seo HY, Kim YA, Oh IH, Lee YH, Yoon SJ. The economic burden of epilepsy in Korea, 2010. Journal of Preventive Medicine and Public Health. 2013;46(6):293.   DOI
25 Lee J, Kim KT, Cho YW. Self-management in epilepsy. Epilia: Epilepsy and Community. 2020;2(1):11-6.
26 Heo K. Epilepsy: Drug Treatment. Journal of the Korean Medical Association. 2003;46(4):287-97.   DOI
27 SO Nam. The Pharmacotherapy of Childhood Epilepsy. Korean Journal of Pediatrics. 2004;47(8):821-6.
28 Lin TK, Chen SD, Lin KJ, Chuang YC. Seizureinduced oxidative stress in status epilepticus: Is antioxidant beneficial? Antioxidants. 2020;9(11): 1029.   DOI
29 Heo Jun. Donguibogam. 1613. Donguibogam. publishing Buvin puvlishers CO. 2005: p.206-9.
30 Choi S. Pharmacological and Molecular studies of Atracylodes japonica Koidzumi on anti-oxidant activity. Kyunghee University Graduate School. 2011.
31 Corvino V, Marchese E, Michetti F, Geloso MC. Neuroprotective strategies in hippocampal neurodegeneration induced by the neurotoxicant trimethyltin. Neurochemical research. 2013;38(2): 240-53.   DOI
32 Park CS, Kim DH. Biological activities of extracts from Scutellaria baicalensis, Zizyphus jujuba and Atractylodes macrocephala. The Korea Journal of Herbology. 2008;23(3):41-51.
33 Textbook Compilation Committee of National Universitiy of Korean Medicine. Herbology. Seoul: Yeonglimsa. 2007:p579-80.
34 Lee S, Yang M, Kim J, Kang S, Kim J, Kim JC, et al. Trimethyltin-induced hippocampal neurodegeneration: A mechanism-based review. Brain research bulletin. 2016;125:187-99.   DOI
35 Besser R, Kramer G, Thumler R, Bohl J, Gutmann L, Hopf H. Acute trimethyltin limbic-cerebellar syndrome. Neurology. 1987;37(6):945-50.   DOI