• 한국수산과학회지
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• 제56권5호
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• pp.708-715
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• 2023

Hybridization is a major production method used to combine beneficial traits from two different species to obtain a potentially dominant trait. In China, Takifugu obscurus and T. rubripes were artificially crossed, and the resulting hybrids had an average body weight 38.06-8.93% higher than that of the parental species, which enabled the hybrids to be grown in freshwater. This study aimed to provide the basic data necessary for the classification of T. obscurus♀×T. rubripes♂ hybrids in terms of economic value and market potential. Morphological comparing the morphology of hybrids and parental species, we discovered that the hybrids had intermediate traits of the parental species. In morphometrics, the hybrid index (HI) value of head length against standard length was close to the trait of T. rubripes, and the HI values of preanal length and predorsal length were close to those of T. obscurus; however, the HI values of nasal length, snout length, length of anal fin, length of pectoral fin, caudal peduncle depth and caudal peduncle length were found to be unique characteristics of the hybrids. Regarding molecular analysis, a 99.8% nucleotide sequence similarity was found between the hybrid and T. obscurus.

• 한국산림과학회지
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• 제94권4호통권161호
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• pp.258-263
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• 2005

경남 거제지역에 자생하고 있는 붉은고로쇠나무의 흉고직경급에 따른 수액채취량과 성분을 분석하였다. 붉은고로쇠나무의 수액채취 가능기간은 17일간이었으며, 총수액채취량은 60본에서 193 l이었다. 총수액채취량에 대해 각 20본의 소 중 대경목이 각각 40 l(21%), 49 l(25%), 104 l(54%)가 채취되어 흉고직경이 증가할수록 수액채취량이 많아지는 경향을 나타내었다. 이는 수관폭과 근계발달에 기인하는 것으로 추정된다. 수액채취량은 주야간의 온도격차가 클 때 많은 것으로 나타났다. 붉은고로쇠나무 수액의 pH, 고형분, 당도는 각각 6.5, 2.40%, 1.99%로 측정되었으며, 유리당은 자당 함량이 19.9 g/L가 검출되었으나 포도당, 과당, 맥아당은 검출되지 않았다. 붉은고로쇠나무 수액은 총 7종의 무기성분이 검출되었으며 Ca과 Mg의 함량이 각각 441 mg/l 383 mg/l으로 나타났다.

• 한국식품위생안전성학회지
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• 제15권1호
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• pp.46-50
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• 2000

우리나라에서 유통되고 있는 자주복의 독성을 조사하기 위하여 1995년 1월, 4월 및 9월에 부산시내 자갈치 어시장에서 총 27개체를 구입하여 부위별 독성을 조사하였다. 간장의 경우 독성이 10MU/g 이상인 유독개체 출현율은 14.8%, 최고 독성치는 160 MU/g, 평균 독성치는 7$\pm$6 MU/g(평균치$\pm$표준오차)이었다. 생식소의 경우는 각각 16.7%, 600 MU/g 및 50$\pm$35 MU/g 이었고, 껍질에서는 각각 14.8%, 26 MU/g 및 5$\pm$1MU/g이었다. 그러나 근육에서는 독이 검출되지 않았다. 계절에 따른 독성은 1월 및 4월의 일부시료에서는 유독부위가 있었으나 9월에는 시료어의 전부위에서 독성이 검출되지 않았다. 자주복의 독성은 부위나 계절에 따른 차이가 있지만 주 가식부인 근육과 껍질에서는 거의 독이 검출되지 않거나 약한 독성이 검출되어 식품 위생상 비교적 안전한 종으로 판단되었다.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.373.1-373.1
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• 2002

Twenty Artemisia species are distributed in South Korea and rich in terpenoids. Artemisia rubripes (Compositae) has been used as a Korean traditional medicine for stomachache. vomiting. diarrhea and hemostatic agent. The antimutagenic effect and essential oils of Artemisia rubripes were reported, but phytochemical study has not been fully investigated. As part of our systematic study on the terpene constituents of Artemisia species. we have investigated A. rubripes (1 kg) collected at Dae-Kwan ryung. Gangwon Province on Aug. 1997. (omitted)

• 한국발생생물학회지:발생과생식
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• 제1권1호
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• pp.29-36
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• 1997

Experiments were performed to study the effects of diluents, cryoprotectant, equilibration time, thawing temperature and addition of BSA and egg yolk. Among the various diluents, Alsever's solution was the best for sperm cryopreservation. A combination of Alsever's solution and 15% ethylene glycol showed the better results than others did. Sperm activity indection and survival rate gradually decreased with the equilibration time. The appropriate thawing temperature was 30 ${\pm}1^{\circ}$C. These results indicate that sperm cryopreservation methods can be developed in tiger puffer.

• 한국농림기상학회지
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• 제6권4호
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• pp.265-271
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• 2004

본 연구는 경남 거제지역 붉은고로쇠나무림의 생육 적지에 대한 기초자료를 제공하고자 토양특성과 식생구조 등 생태적 환경을 조사하였다 붉은고로쇠나무는 해발 150∼300m의 북사면에 주로 분포하며, 암반노출 도는 80% 이상이었다 붉은고로쇠나무의 토양 pH, 유기물 및 전질소 함량은 각각 5.17, 8.7%, 0.38%로 나타났다 유효인산은 낮은 토양 pH로 인해 15.7ppm으로 나타났다. 전 층위에 공통적으로 출현한 종은 붉은고로쇠나무, 나도밤나무, 느티나무, 때죽나무, 사람주나무, 비목, 참회나무 등 1종이었다. 각 충위별 중요치는 상층에서 붉은고로쇠나무가 가장 높았고, 느티나무, 졸참나무, 합다리나무 순으로 높게 나타났다 중층과 하층에 있어서는 사람주나무와 나도밤나무의 중요치가 상대적으로 높게 나타났다 층위별 종다양도는 상층 0.933, 중층 0.931, 하층 1.638이며 균재도는 상층 0.706, 중충 0.894 그리고 하층 0.959로 나타났다. 이들 결과는 우리나라의 거제도에만 자생하고 있는 붉은 고로쇠나무의 토양환경과 식생구조에 관한 기초적인 자료로 활용될 수 있을 것이다.

• Archives of Pharmacal Research
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• 제27권10호
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• pp.1016-1019
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• 2004

The chromatographic separation of a methylene chloride extract of Artemisia rubripes led to the isolation of a new sesquiterpene lactone (3), together with four known compounds, a coumarin (2) and three terpenes (1, 4, and 5). Their structures were characterized to be $1{\beta},6{\alpha}$- dihydroxy-4(15)-eudesmene (1), scopoletin (2), $1{\alpha},4{\beta}-dihydroxy-8{\alpha}$-acetoxy-guaia-2,10(14), 11(13)-triene-6,12-olide (3), $1{\alpha},4{\beta}$ -dihydroxy-8${\alpha}$-acetoxy-guaia-2,9,11(13)-triene-6,12-olide (4), and $\beta$ -sitosterol-3-O-${\beta}$-D-glycoside (5) by spectroscopic means.

• 한국수산과학회지
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• 제31권3호
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• pp.353-358
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• 1998

Experiments were performed to find out the physico-chemical properties of milt, and morphological changes of cryopreserved spermatozoa in tiger puffer, Takifugu rubripes. The average number of sperm and spermatocrit in milt stripped were $9.81{\pm}0.34{\times}10^{10}/m{\ell}$ and $97.8{\pm}0.8$, respectively. While total lipid concentration from seminal fluid was higher than that from sperm, total protein concentration from sperm was higher than that from seminal fluid, Na and K concentrations in sperm and those in seminal fluid were similar each other, However, glucose from sperm and seminal fluid were not detectable. Spermatozoon of tiger puffer was consisted of head, middle Piece and tail. Size of head showing horseshoe shape was $0.65{\pm}0.10{\mu}m$ in diameter and $1.35{\pm}0.30{\mu}m$ in length. The head fully containing chromatin did not have acrosome. Mitochondrion in middle piece was $0.2{\mu}m$ in average diameter and flagellum showed 9+2 structure. A few of cryopreserved spermatozoa showed morphologically loose or swollen plasma membranes.

• 한국수산과학회지
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• 제51권4호
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• pp.404-410
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• 2018

Takifugu rubripes, T. chinensis, T. pseudommus and an unidentified species were morphologically and genetically studied. Morphology was compared using external morphology and the genetic analysis was conducted using mitochondrial DNA cytochrome oxidase I (COI) and cytochrome b (Cyt b). The morphological characteristics of T. rubripes, T. chinensis and T. pseudommus were confirmed, as reported previously. The unidentified species was confirmed to have the characteristics of T. rubripes and T. chinensis. The COI sequences of the four species were 99-100% similar; genetic distance was d=0.0000 and Cyt b genetic distance was d=0.0000-0.00834. Species in the phylogenetic tree belonging to the same group could not be classified genetically. In conclusion, the unidentified species was considered to be a hybrid between T. rubripes and T. chinensis.

• 한국수산과학회지
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• 제49권4호
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• pp.467-473
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• 2016

Several of our recent studies have focused on mass production of the marine Cladoceran Diaphanosoma celebensis, which can serve as a feed substitute for Artemia nauplii in the culture of larval marine fish. We compared the growth and survival rates of tiger puffer Takifugu rubripes fed D. celebensis with those fed Artemia. The group fed D. celebensis showed an increase in growth from 0.10 g to 1.78 g, while the group fed enriched Artemia had a growth from 0.10 g to 0.92 g. The survival rate of fish fed D. celebensis was 86.7% while that of fish fed enriched Artemia was 51.1%, indicating that the former group was superior to the latter both in growth and survival rate. Fatty acid analysis revealed that D. celebensis fed Nannochloropsis oculata and Schizochytrium sp. had an eicosapentaenoic acid (EPA) content of 16.42% and a docosahexaenoic acid (DHA) content of 3.93%. Meanwhile, juvenile tiger puffer fed D. celebensis had an EPA content of 9.12% and a DHA content of 10.69%. Juvenile tiger puffer fed D. celebensis had a similar n-3 highly unsaturated fatty acid content to that of tiger puffer fed enriched Artemia which had an EPA content of 5.82% and a DHA content of 15.90%, indicating that no additional enrichment was required.