Browse > Article
http://dx.doi.org/10.5657/KFAS.2018.0404

Morphological and Molecular Comparison among Three Species and One Unidentified Takifugu Species  

Baek, Jeong-Ik (Fisheries Resources Research Center, National Institute of Fisheries Science)
Han, Kyeong-Ho (Department of Marine Technology, Chonnam National University)
Lee, Seong-Hoon (Department of Marine Technology, Chonnam National University)
Kim, Jin-Koo (Department of Marine Biology, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.51, no.4, 2018 , pp. 404-410 More about this Journal
Abstract
Takifugu rubripes, T. chinensis, T. pseudommus and an unidentified species were morphologically and genetically studied. Morphology was compared using external morphology and the genetic analysis was conducted using mitochondrial DNA cytochrome oxidase I (COI) and cytochrome b (Cyt b). The morphological characteristics of T. rubripes, T. chinensis and T. pseudommus were confirmed, as reported previously. The unidentified species was confirmed to have the characteristics of T. rubripes and T. chinensis. The COI sequences of the four species were 99-100% similar; genetic distance was d=0.0000 and Cyt b genetic distance was d=0.0000-0.00834. Species in the phylogenetic tree belonging to the same group could not be classified genetically. In conclusion, the unidentified species was considered to be a hybrid between T. rubripes and T. chinensis.
Keywords
Takifugu; Unidentified species; Comparison study; Morphology; Genetics;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Abe T and Tabeta O. 1984. Pufferfishes Available in Japan. An illustrated guide to their identification Central law publication, Tokyo, Japan, 85.
2 Baek. 2017. Taxonomy and Molecular Phylogeny of the Tetraodontoidei (Teleostei:Tetraodontiformes). Ph. D Thesis, Chonnam National University, Yeosu, Korea.
3 Brykov VlA and Podlesnykh AV. 2001. Comparative study of mitochondrial DNA in two greening species (Hexagrammidae: Pisces) and their hybrids from Peter the Great Bay (Sea of Japan). Anim Genet 12, 1663-1666.
4 Grant PR and Grant BR. 1992. Hybridization of bird species. Science 256, 193-197.   DOI
5 Han KH. 1995. Morphology, osteology and phylogeny of the fishes of the Family Tetraodontidae (Teleostei; Tetraodontiformes). Ph. D Thesis, National Fisheries University of Busan, Busan, Korea, 205.
6 Han KH, Baek JI, Shin LS, Kim HJ, Yoon BI, Hwang JH and Lee SH. 2017. Morphological Description of Three Species of Pufferfishes (Tetraodontidae) from India. Korean J Fish Aquat Sci 50, 77-84. http://dx.doi.org/10.5657/KFAS.2017.0077.   DOI
7 Han KH and Kim YU. 1998a. Taxonomic Revision of the Genus Takifugu (Teleostei; Tetraodontidae) from Korea. Bull Yosu Nat'l Univ 12, 563-589.
8 Han KH and Kim YU. 1998b. Taxonomic Revision of the Genera Logocephalus and Pleurancanthus (Teleostei; Tetraodontidae) from Korea. Bull Yosu Nat'l Univ 12, 591-604.
9 Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111-120.   DOI
10 Kim IS and Lee WO. 1990. Synopsis of the Suborder Tetraodontoidei (Pisces; Tetraodontiformes) from Korea. Korea J Ichthyol 2, 1-27.
11 Kwun HJ and Kin JK. 2010. Occurrence of Natural Hybrid between Oplegnathus fasciatus and Oplegnathus punctatus from the South Sea of Korea. Korea J Ichthyol 22, 201-205.
12 Lee IR, Yang H, Kim JH, Kim KY and Bang IC. 2009. Identification of a natural hybrid between the striped spine loach Cobitis tetralineata and the king spine loach Iksookimia longicorpa by analyzing mitochondrial COI and Nuclear RAG1 sequences. Korea J Ichthyol 21, 287-290.
13 Reza MS, Furukawa S, Mochizuki T, Matsumura H and Watabe S. 2008. Genetic comparison between torafugu Takifugu rubripes and its closely related species karasu Takifugu chinensis. J Soc Fisheries Science 17, 743-754. https://doi.org/10.1111/j.1444-2906. 2008. 01585.X.   DOI
14 Lee WO. 1993. A Systematic study of the family Tetraodontidae (Pisces: Tetraodontiformes) from Korea. Ph.D. Thesis, Chonbuk National University, Jeongju, Korea.
15 Masuda H, Amaoka K, Araga C, Uyeno T and Yoshino T. 1984. The fishes of the Japanese archipelago. Tokai University, Tokyo, Japan, 437.
16 Nelson JS. 2006. Fishes of the world (4th ed.). John Wiley and Sons, New York, NY, U.S.A., 601.
17 Song L, Liu B, Xiang J and Qian PY. 2001. Molecular Phylogeny and Species Identification of Pufferfish of the Genus Takifugu (Tetraodontiformes, Tetraodontidae). Mar Biotechnol 3, 398-406. https://doi.org/10.1007/S10126-001-0006-5.   DOI
18 Tamura K, Dudley J, Nei M and Kumar S. 2013. MEGA4: Molecular Evolutionary GeneticsAnalysis (MEGA) software version 6.0. Mol Biol Evol 24, 1596-1599.
19 Yun YE, Lee IR, Park SY, Kang EJ, Kim EO, Yang SK, Nam YK and Bang IC. 2009. Genetic identification of hybrids between Rhodeus uyekii and R. notatus by sequence analysis of RAG-1 gene. J Aquaculture 22, 79-82.