• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.5
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• pp.708-715
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• 2023

Hybridization is a major production method used to combine beneficial traits from two different species to obtain a potentially dominant trait. In China, Takifugu obscurus and T. rubripes were artificially crossed, and the resulting hybrids had an average body weight 38.06-8.93% higher than that of the parental species, which enabled the hybrids to be grown in freshwater. This study aimed to provide the basic data necessary for the classification of T. obscurus♀×T. rubripes♂ hybrids in terms of economic value and market potential. Morphological comparing the morphology of hybrids and parental species, we discovered that the hybrids had intermediate traits of the parental species. In morphometrics, the hybrid index (HI) value of head length against standard length was close to the trait of T. rubripes, and the HI values of preanal length and predorsal length were close to those of T. obscurus; however, the HI values of nasal length, snout length, length of anal fin, length of pectoral fin, caudal peduncle depth and caudal peduncle length were found to be unique characteristics of the hybrids. Regarding molecular analysis, a 99.8% nucleotide sequence similarity was found between the hybrid and T. obscurus.

• Journal of Korean Society of Forest Science
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• v.94 no.4 s.161
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• pp.258-263
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• 2005

Amounts of sap collected by DBH class and contents of mineral and free sugar in the sap of Acer mono for. rubripes indigenous in Geojedo were investigated. The collectable periods of sap of A. mono for. rubripes were 17 days and the total amount of sap was 193 l from 60 trees. Sap of A. mono for. rubripes were increased with increasing DBH, being 40 l (21 %), 49 l (25%) and 104 l (54%) from small, middle and large diameter tree, respectively. This may be due to increase in crown width and extention of root system. Amounts of sap was affected by temperature fluctuation between day and night. The pH and the contents of solid and sugar in the sap of A. mono for. rubripes were 6.5 and 2.40% and 1.99%, respectively. Free sugar determined in the sap was sucrose with 19.9 g/l, but glucose, fructose and maltose were not detected. The sap of A. mono for. rubripes contained seven kinds of minerals and the prominent minerals were Ca and K with their concentration being 441 mg/l and 383 mg/l, respectively.

• Journal of Food Hygiene and Safety
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• v.15 no.1
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• pp.46-50
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• 2000

Totally, twenty seven specimens of tiger puffer, Fugu rubripes rubripes were collected at Jagalchi fish market in Pusan, Korea during January, April and September in 1995. Anatomical distribution of pufferfish toxin in tiger puffer was examined by mouse bioassay. The frequency rate of toxic specimens containing $\geq$ 10 MU/g was 14.8％ in liver; 16.7％ in gonad; and 14.8％ in skin, and no toxin was detected in muscle. The highest toxin level found was 160 MU/g in liver, 600 MU/g in gonad and 26 MU/g in skin, and each average toxin level (mean$\pm$ standard error) was 7$\pm$6, 50$\pm$35 and 5$\pm$1 MU/g, respectively. Some specimens collected in January and April were toxic, while none of the specimens collected in September showed its toxicity. Although toxicity of tiger puffer showed the seasonal variation, tested tiger puffer was evaluated as a safe seafood fur consumption, in that an acceptable level of toxin was found in the edible muscle and skin.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.373.1-373.1
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• 2002

Twenty Artemisia species are distributed in South Korea and rich in terpenoids. Artemisia rubripes (Compositae) has been used as a Korean traditional medicine for stomachache. vomiting. diarrhea and hemostatic agent. The antimutagenic effect and essential oils of Artemisia rubripes were reported, but phytochemical study has not been fully investigated. As part of our systematic study on the terpene constituents of Artemisia species. we have investigated A. rubripes (1 kg) collected at Dae-Kwan ryung. Gangwon Province on Aug. 1997. (omitted)

• Development and Reproduction
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• v.1 no.1
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• pp.29-36
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• 1997

Experiments were performed to study the effects of diluents, cryoprotectant, equilibration time, thawing temperature and addition of BSA and egg yolk. Among the various diluents, Alsever's solution was the best for sperm cryopreservation. A combination of Alsever's solution and 15% ethylene glycol showed the better results than others did. Sperm activity indection and survival rate gradually decreased with the equilibration time. The appropriate thawing temperature was 30 ${\pm}1^{\circ}$C. These results indicate that sperm cryopreservation methods can be developed in tiger puffer.

• Korean Journal of Agricultural and Forest Meteorology
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• v.6 no.4
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• pp.265-271
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• 2004

This study was carried out to furnish basic information on the habitat through soil condition and vegetation structure of an Acer mono for. rubripes stand in Geoje, Gyeongnam Province. Soil pH of the A. mono for. rubripes stand was 5.17. The contents of organic matter, total N and available P205 were 8.7%,0.38%, 15.7ppm, respectively. A. mono for. rubripes, Meliosma oldhamii, Zelkova serrata, Styrax japonica, Sapium japonicum, Lindera erythrocarpa and Euonymus oxyphyllus appeared in all layers. The importance values of A. mono for. rubripes, Z. serrata and Quercus serrata in the upper layer were high, S. japonicum and M. myriantha were high in the middle layer and lower layer, respectively. Species diversity and evenness ranged from 0.931 of the middle layer to 1.638 of the lower layer, and from 0.706 of the upper layer to 0.959 of the lower layer, respectively. The study results provide basic information on soil condition and vegetation structure of the Acer mono for. rubripes stand native to Geoje, Korea.

• Archives of Pharmacal Research
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• v.27 no.10
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• pp.1016-1019
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• 2004

The chromatographic separation of a methylene chloride extract of Artemisia rubripes led to the isolation of a new sesquiterpene lactone (3), together with four known compounds, a coumarin (2) and three terpenes (1, 4, and 5). Their structures were characterized to be $1{\beta},6{\alpha}$- dihydroxy-4(15)-eudesmene (1), scopoletin (2), $1{\alpha},4{\beta}-dihydroxy-8{\alpha}$-acetoxy-guaia-2,10(14), 11(13)-triene-6,12-olide (3), $1{\alpha},4{\beta}$ -dihydroxy-8${\alpha}$-acetoxy-guaia-2,9,11(13)-triene-6,12-olide (4), and $\beta$ -sitosterol-3-O-${\beta}$-D-glycoside (5) by spectroscopic means.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.3
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• pp.353-358
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• 1998

Experiments were performed to find out the physico-chemical properties of milt, and morphological changes of cryopreserved spermatozoa in tiger puffer, Takifugu rubripes. The average number of sperm and spermatocrit in milt stripped were $9.81{\pm}0.34{\times}10^{10}/m{\ell}$ and $97.8{\pm}0.8$, respectively. While total lipid concentration from seminal fluid was higher than that from sperm, total protein concentration from sperm was higher than that from seminal fluid, Na and K concentrations in sperm and those in seminal fluid were similar each other, However, glucose from sperm and seminal fluid were not detectable. Spermatozoon of tiger puffer was consisted of head, middle Piece and tail. Size of head showing horseshoe shape was $0.65{\pm}0.10{\mu}m$ in diameter and $1.35{\pm}0.30{\mu}m$ in length. The head fully containing chromatin did not have acrosome. Mitochondrion in middle piece was $0.2{\mu}m$ in average diameter and flagellum showed 9+2 structure. A few of cryopreserved spermatozoa showed morphologically loose or swollen plasma membranes.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.4
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• pp.404-410
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• 2018

Takifugu rubripes, T. chinensis, T. pseudommus and an unidentified species were morphologically and genetically studied. Morphology was compared using external morphology and the genetic analysis was conducted using mitochondrial DNA cytochrome oxidase I (COI) and cytochrome b (Cyt b). The morphological characteristics of T. rubripes, T. chinensis and T. pseudommus were confirmed, as reported previously. The unidentified species was confirmed to have the characteristics of T. rubripes and T. chinensis. The COI sequences of the four species were 99-100% similar; genetic distance was d=0.0000 and Cyt b genetic distance was d=0.0000-0.00834. Species in the phylogenetic tree belonging to the same group could not be classified genetically. In conclusion, the unidentified species was considered to be a hybrid between T. rubripes and T. chinensis.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.4
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• pp.467-473
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• 2016

Several of our recent studies have focused on mass production of the marine Cladoceran Diaphanosoma celebensis, which can serve as a feed substitute for Artemia nauplii in the culture of larval marine fish. We compared the growth and survival rates of tiger puffer Takifugu rubripes fed D. celebensis with those fed Artemia. The group fed D. celebensis showed an increase in growth from 0.10 g to 1.78 g, while the group fed enriched Artemia had a growth from 0.10 g to 0.92 g. The survival rate of fish fed D. celebensis was 86.7% while that of fish fed enriched Artemia was 51.1%, indicating that the former group was superior to the latter both in growth and survival rate. Fatty acid analysis revealed that D. celebensis fed Nannochloropsis oculata and Schizochytrium sp. had an eicosapentaenoic acid (EPA) content of 16.42% and a docosahexaenoic acid (DHA) content of 3.93%. Meanwhile, juvenile tiger puffer fed D. celebensis had an EPA content of 9.12% and a DHA content of 10.69%. Juvenile tiger puffer fed D. celebensis had a similar n-3 highly unsaturated fatty acid content to that of tiger puffer fed enriched Artemia which had an EPA content of 5.82% and a DHA content of 15.90%, indicating that no additional enrichment was required.