• Title/Summary/Keyword: 4D-Var

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Dictyostelids in Mt. Paektu (백두산의 딕티오형 세포성 점균)

  • 심규철;강경미;장남기
    • The Korean Journal of Ecology
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    • v.21 no.5_2
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    • pp.557-565
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    • 1998
  • The occurrence and distribution of dictyostelid cellualr slime molds were investigated in forests of Mt. Paektu. Fourteen species were isolated from forest soils of thirteen sites as follows Dictyostelium minutum, D. brefeldianum, D. crassicaule, D. capitatum, D. mucoroides var. stoloniferum, D. macrocephalum, D. mucoroides, D. septentrionalis, D. purpureum, D. aureo-stipes, P. violaceum, P. pallidum and two unidentified speceis. Mean total clones and species found were 4,415.69 No./g and 2.92, respectively. Dominant species were D. minutum, D. brefeldianum and D. crassicaule in this study area, and first widespread species was D. minutum. All sepecies that found in Mt. Paektu were isolated from forests of South Korea. But dominant species was different from those of South Korea. Total clones and species were more in subalpine Eurya japonica than in low elevated abies nephrolepis. It was thought that distribution of dictyostelids was related to soil microenvironments by vegetation, not or not more elevation.

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Studies on Goucoalkaloids in Plants I. High Performance Liquid Chromatographic Analysis of Glucoalkaloids in Periderm and Cortex of Potato (Solanum tuberosum var. May Queen) (식물체(植物體)의 Glucoalkaloids 에 관(關)한 연구(硏究) 제1보(第1報) 고속액체(高速液體)크로마토그래피에 의한 감자(Solanum tuberosum var. May Queen)의 피층부(皮層部) 및 수질부(髓質部)의 Glucoalkaloids 측정(測定))

  • Hwang, Chun-Sun;Lee, Sung-Woo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.11 no.3
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    • pp.1-4
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    • 1982
  • By high performance liquid chromatography, separation and quantification of glucoalkaloids $({\alpha}-chaconine$and${\alpha}-solanine)$ from potato (Solanum tuberosum, var. May Queen) was established using periderm and cortex. $Nucleosil-NH_2\;(10{\mu}m)$ was packed in two stainless steel columns ($4.0\;ID{\times}15\;cm$ and $4.0\;ID{\times}25\;cm$) which were connected in sequence and eluted with the mixture of tetrahydrofuran, phosphoric acid buffer and acetonitrile (50 : 25 : 25, v/v/v) at the flow rate of 1ml/min. and the absorbance were read at 208 nm. Retention time was 6.92 min. for ${\alpha}-chaconine$ and 10.96 min. for ${\alpha}-solanine$ with complete separation. This method took 12 min. per sample and seemed best. ${\alpha}-Chaconine$ and ${\alpha}-solanine$ were found much in periderm than in cortex.

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Propagation of Endangered Species, Daphne pseudomezereum var. koreana via in vitro Bud Culture (멸종위기종 두메닥나무(Daphne pseudomezereum var. koreana)의 줄기 기내배양을 통한 식물체 생산)

  • Chu, Yerin;Park, Sanghee;Cheong, Eun Ju
    • Journal of Korean Society of Forest Science
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    • v.109 no.2
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    • pp.189-194
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    • 2020
  • Daphne pseudomezereum var. koreana is native to Korea and is distributedin Kangwon-do, Jeollabuk do, and Gyeongsang-do. This economically valuable species has experienced a dramatic decrease in natural habitat due to climate change and is difficult to cultivate. In this study, we investigate a mass propagation method for D. pseudomezereum through in vitro culture and genetic resource preservation.WPM medium was better than the MS medium for shoot growth. As a result, we compared the shoot number and length of apical (W/AP) and non-apical shoots (W0/AP) with BA and GA3 treatments in WPM medium. Their shoots and length grew well in both BA 8ìM + GA38ìM-treated apical shoot and without-apical shoot. NAA did not effectively induce rooting of the in vitro plantlet.

Herbicide Resistance in Plant Tissue Culture (식물체(植物體) 조직배양(組織培養)과 제초제(除草劑) 저항성(抵抗性))

  • Kim, Kil-Ung
    • Korean Journal of Weed Science
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    • v.5 no.1
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    • pp.9-13
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    • 1985
  • This study was conducted to evaluate herbicide resistant plant through tissue culture. Callus was induced from embryos of Echinochloa crusgalli Beauv. (var, oryzicola Ohwi, var. caudata Kitagawa and var, crusgalli). An optium medium for callus induction and succinate dehydrogenase activity in inducted callus were detected and callus growth of various varieties of Echinochloa crusgalli was assessed under the treatment of various rates of butachlor[N-(butoxymethyl)-2-chloro-N-(2,6-diethylphenyl)acetamide]. MS medium seemed to be the most appropriate to induce callus from the embryos of varieties of E. crusgalli by using 2,4-D about 5.5mg/l as a hormone source. The activity of succinate dehydrogenase in inducted callus showed positive reaction against to TTC(2,3,5-triphenyltetrazolium chloride) regardless of concentrations of butachlor and varieties of E. crusgalli, indicating that all the callus induced were alive. The callus growths derived from seeds of E, cnesgalli were greatly affected by various rates of butachlor and were completely inhibited at the highest concentration of butachlor, $10^{-3}M$, regardless of varieties of E. crasgalli. $10^{-6}M$ of butachlor inhibited 24.6% of the callus growth of E. crusgalli Beauv, var. oryzicola Ohwi, while E. crusgalli Beauv. var. crusgalli showed 42% of inhibition, showing that there was difference in response of varieties of E. crusgalli Beauv. to butachlor.

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Ecological Studies on the Forest Vegetation in the Mt. Joghe (조계산(曹溪山) 삼림식생(森林植生)의 생태학적(生態學的) 연구(硏究))

  • Chang, Seok Mo
    • Journal of Korean Society of Forest Science
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    • v.80 no.1
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    • pp.54-71
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    • 1991
  • To classify and analyze the forest communities and their structures, the vegetation in Mt. Joghe was investigated from July, 1980 to August, 1989. The results obtained are as follow ; 1. A total of 750 kinds of vascular plant(49 orders, 122 families, 434 genera, 627 species, 1 subspecies, 111 varieties and 11 forma)were observed in Mt. Ioghe. The newly observed plant species were Dioscorea quingueloba, Spiranthes sinensis, Cephalanthera falcata, Angelica gigas, Clematis patents, Paeonia obovata, Hibiscus mulabilis, Ainsliaea acerifolia, Dictamnus dasycarpus, Cynachum ascyrifolia, Vaccinium koreanum, Erythrortium japonicum, Indigofera kirilowii (17species), Broussonetia kazinoki var, humillis, Euonymus, fortunei var. radicans, Juniperus communis var, nippnnica, Callicarpa japonica var. radicans, Joniperus communis var. rzipponica, Callicarpa japonica var. taquetii (4 varieties) and L indera obtusiloba for. billosum (1 forma). 2. The life spectrum of flora in Mt. Joghe was classified into $CH-D_1-R_5-e$ type. Distribution area was identical to Southern type by Nakai, Lee, and Yim. A few subtropical species were also observed. 3. Simpson's species diversity index(Ds) was 0.9 and Shannon-Weiner's diversity index (H') was 1.004. These indice suggest that the vegetation in Mt. Joghe is of complicated forest communities. 4. Pte-Q was 1.81 which was higher than the nationwide mean of 1.68. Urbanization Index (UI) was 28.75 for naturalized plant species, and 17.49 for exotic woody plant species, which were similar to those of Mt. Baekun and Mt. Naejang. 5. The forest vegetation of Mt. Joghe was grouped in 3 vegetation types : 7 natural plant Communities dominated by Quercus serrat, Quercus acutissima, Quercus variabilis, Carpinus laxiflora, Pinus derasiflora and Platycarya strobilacea, 8 substitutional plant communities Styrax japonica, Stewartia koreana, Lindera erytlrrocarpa, Zelkova serrata, Rhtrs chinensis, Controversa, and Frzrxirtus manrlshurica, and 7 plantation Communities composed of Pinus koraiensis, Pinus rigida, Magnolia nbnvata, Chamecyparis obkrsa, Larie ieptolepis, Castanea crenata and Cryptomeria japonica. 6. Actual vegetation maps and profile diagrams were made by phytosocialogical classification. 7. As the important and unique species in Mt. Joghe, Lindera sericea, Penicaria tilitorme, Hex macropoda, Hex macropoda for. pseudo-macropoda, Steroartia koreana, Adenopkora palustris and Corylop.,is coreana, which were also seported by Lee(1977), Kim and Yark(1989), were identified and Vaccinium coreanum, Cremastra appendiculinium, Juniperus comminis van. nipponica, Cephalanthera falcata, Broussortetia kazinoki var. humilis, paeonia obovata, Deutzia prunifolia, Dictamnus dasyarpus, Angelica gigics and Bupleurum falcatum were odditionally observed.

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A taxonomic study on Daucus species vegetated in islands off the Korean Peninsula (한반도 도서 지역에 서식하는 당근류의 분류학적 고찰)

  • Lee, Byoung-Yoon;Kwon, Sun Gyo;Oh, Kyoung-Hee
    • Korean Journal of Plant Taxonomy
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    • v.34 no.4
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    • pp.287-295
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    • 2004
  • Daucus species vegetated in the island Dae-heug-san-do off the Korean Peninsula has been recorded as Daucus littoralis Sibth. et Sm. var. koreana Nakai, endemic to Korea in several flora. However, the name was revealed as a naked name, nomen nudum, because the valid publication and type specimens were found anywhere. Comparative observation on morphological characters between the Korean taxon of Daucus and D. littoralis reveals that the Korean vegetated in the island Dae-heug-san-do is not the D. littoralis, but the D. carota L. subsp. carota. The Korean name of the latter is designated newly as San-dang-geun. The new korean name, Seon-dang-geun, of the unrecorded taxon, D. carota subsp. maritimus (Lam.) Batt., is also given.

Decomposition of leaf litter of some evergreen broadleaf trees in Korea

  • Lee, Kyung Eui;Cha, Sangsub;Lee, Sang Hoon;Shim, Jae Kuk
    • Journal of Ecology and Environment
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    • v.38 no.4
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    • pp.517-528
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    • 2015
  • Litter decomposition is an important process in terrestrial ecosystem. However, studies on decomposition are rare, especially in evergreen broadleaf trees. We collected the leaf litter of five evergreen broadleaf trees (Daphniphyllum macropodum, Dendropanax morbifera, Castanopsis cuspidata var. thunbergii, Machilus thunbergii and Quercus acuta), and carried out a decomposition experiment using the litterbag method in Ju-do, Wando-gun, Korea for 731 days from December 25, 2011 to December 25, 2013. Among the five experimental tree species, C. cuspidata var. thunbergii distribution was limited in Jeju Island, and D. macropodum was distributed at the highest latitude at Mt. Baekyang (N 35°40′). About 2% of the initial litter mass of D. macropodum and D. morbifera remained, while 20.9% remained for C. cuspidata var. thunbergii, 30.4% for M. thunbergii, and 31.6% for Q. acuta. D. macropodum litter decayed four times faster (k = 2.02 yr-1) than the litter of Q. acuta (k = 0.58 yr-1). The decomposition of litter was positively influenced by thermal climate such as accumulated mean daily air temperature (year day index) and precipitation, as well as by physical characteristics such as thickness (R2=0.939, P = 0.007) and specific leaf area (SLA) (R2 = 0.964, P = 0.003). The characteristics of chemical composition such as lignin (R2 = 0.939, P = 0.007) and water-soluble materials (R2 = 0.898, P = 0.014) showed significant correlations with litter decomposition. However, the nutrients in litter showed complicated species-specific trends. The litter of D. macropodum and D. morbifera had fast decomposition despite their low nitrogen concentration and high C/N ratio. This means that the litter decomposition was more strongly affected by physical characteristics than chemical composition and nutrient content. On the other hand, the litter of Q. acuta which had the slowest decay rate had a high amount of N and low C/N ratio. Thus, the decomposition of Q. acuta litter was more affected by the P content of the litter than the N content, although all litter had similar physical characteristics.

Physiological and Anatomical Studies of Quinclorac Action (Quinclorac의 작용성(作用性)에 대한 연구(硏究))

  • Hong, S.Y.;Lee, I.J.;Kim, K.U.;Shin, D.H.;Lee, C.N.
    • Korean Journal of Weed Science
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    • v.13 no.1
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    • pp.62-70
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    • 1993
  • There was intraspecific variation in Echinochloa crus-galli var, crus-galli in response to quinclorac, showing that plan height and dry weight of a locally collected barnyardgrass(Chinjupi) from Chinju were 90.5 and 37.8% of the untreated control, while those of a locally collected one(Iripi) from Iri showed 19.1 and 14.4%, respectively. The normal distribution curve was obtained from frequency distribution of 89 rice cultivars as affected by the application rates of quinclorac at 30, 300, and 3,000g ai/ha. Protein patterns(SDS-PAGE) of two barnyardgrasses belonging to E, crus-galli var. crus-galli such as Iripi and Chinjupi were not affected by the quinclorac application, indicating that inhibition of enzyme and/or protein biosynthesis seems to be not the primary action target of quinclorac. Electronmicroscopic observation on the injured leaf of Iripi which is considered as a susceptible one showed prominent membrane disruption. Chuchungbyeo(rice variety) resulted in a greater inhibition of tomato growth than those from Chinjupi or Iripi, indicating a great amount of quinclorac discharged from rice root, Chinjupi which is relatively tolerant to quinclorac than Iripi, discharged more quinclorac causing a greater inhibition of tomato growth.

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Take-all of Wheat and Natural Disease Suppression: A Review

  • Kwak, Youn-Sig;Weller, David M.
    • The Plant Pathology Journal
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    • v.29 no.2
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    • pp.125-135
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    • 2013
  • In agro-ecosystems worldwide, some of the most important and devastating diseases are caused by soil-borne necrotrophic fungal pathogens, against which crop plants generally lack genetic resistance. However, plants have evolved approaches to protect themselves against pathogens by stimulating and supporting specific groups of beneficial microorganisms that have the ability to protect either by direct inhibition of the pathogen or by inducing resistance mechanisms in the plant. One of the best examples of protection of plant roots by antagonistic microbes occurs in soils that are suppressive to take-all disease of wheat. Take-all, caused by Gaeumannomyces graminis var. tritici, is the most economically important root disease of wheat worldwide. Take-all decline (TAD) is the spontaneous decline in incidence and severity of disease after a severe outbreak of take-all during continuous wheat or barley monoculture. TAD occurs worldwide, and in the United States and The Netherlands it results from a build-up of populations of 2,4-diacetylphloroglucinol (2,4-DAPG)-producing fluorescent Pseudomonas spp. during wheat monoculture. The antibiotic 2,4-DAPG has a broad spectrum of activity and is especially active against the take-all pathogen. Based on genotype analysis by repetitive sequence-based-PCR analysis and restriction fragment length polymorphism of phlD, a key 2,4-DAPG biosynthesis gene, at least 22 genotypes of 2,4-DAPG producing fluorescent Pseudomonas spp. have been described worldwide. In this review, we provide an overview of G. graminis var. tritici, the take-all disease, Pseudomonas biocontrol agents, and mechanism of disease suppression.

Anti-Inflammatory Activity of Constituents Isolated from Ulmus davidiana var. japonica

  • Zheng, Ming Shan;Yang, Ju-Hye;Li, Ying;Li, Xian;Chang, Hyeun-Wook;Son, Jong-Keun
    • Biomolecules & Therapeutics
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    • v.18 no.3
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    • pp.321-328
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    • 2010
  • Twenty six compounds (1-26) were isolated from the root barks of Ulmus davidiana var. japonica. The anti-inflammatory activity of the isolated compounds were evaluated agai nst the generation of inflammatory chemical mediators in bone marrow-derived mast cells. Among them, compounds 10, 11, 13, 15 and 19 inhibited not only cyclooxygenase-2 dependent prostaglandin $D_2$ generation but also 5-lipoxygenase dependent leukotrien $C_4$ generation in a concentration-dependent manner. In addition, compounds 11, 12, 13, 15 and 19 also inhibited $\beta$-hexosaminidase release, a marker of mast cell degranulation reaction, from bone marrow-derived mast cell. These results suggest that the anti-inflammatory activity of U. davidiana might in part occur by both the inhibition of eicosanoid generations and the degranulation reaction of mast cells.