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Genetic and molecular analysis of the R-mb gene from maize (옥수수 R-mb 유전자의 유전분석과 그의 구조)

  • 윤필용;유삼규;송원용;윤충효;임용표
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.3
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    • pp.161-165
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    • 1997
  • The R-mb locus of maize is one of several genes that encode tissue-specific transcriptional regulator for the anthocyanin biosynthesis in plant parts and the aleurone layer in seeds. We found that the seed pigment frequencies gradually decreased at selfed progenies of the R-mb genetic stocks. In order to analyze the genomic structure of R-mb locus components, genomic Southern blot was performed by using R specific probe, pR-nj:1. Two bands were detected at the size of about 3.9 and 7.75kb. Five R-mb positive clones (mb-II, III, V,Ⅵ, and Ⅶ) were obtained by screening of maize genomic λFIXII library using R specific probe pR-nj:1. We constructed the restriction map of clone mb-II (7.75Kb positive) and mb-Ⅵ (3.9Kb positive), and have compared these with other R locus genes. From genetic and molecular analysis, it is suggested that R-mb complex consists two copy of R elements, and each element shows the paramutagenic and gene silencing effects by the fashion of cis-inactivation.

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A NOTE ON THE MIXED VAN DER WAERDEN NUMBER

  • Sim, Kai An;Tan, Ta Sheng;Wong, Kok Bin
    • Bulletin of the Korean Mathematical Society
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    • v.58 no.6
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    • pp.1341-1354
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    • 2021
  • Let r ≥ 2, and let ki ≥ 2 for 1 ≤ i ≤ r. Mixed van der Waerden's theorem states that there exists a least positive integer w = w(k1, k2, k3, …, kr; r) such that for any n ≥ w, every r-colouring of [1, n] admits a ki-term arithmetic progression with colour i for some i ∈ [1, r]. For k ≥ 3 and r ≥ 2, the mixed van der Waerden number w(k, 2, 2, …, 2; r) is denoted by w2(k; r). B. Landman and A. Robertson [9] showed that for k < r < $\frac{3}{2}$(k - 1) and r ≥ 2k + 2, the inequality w2(k; r) ≤ r(k - 1) holds. In this note, we establish some results on w2(k; r) for 2 ≤ r ≤ k.

THE UNIMODALITY OF THE r3-CRANK OF 3-REGULAR OVERPARTITIONS

  • Robert XiaoJian Hao;Erin YiYing Shen
    • Bulletin of the Korean Mathematical Society
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    • v.61 no.3
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    • pp.621-635
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    • 2024
  • An 𝑙-regular overpartition of n is an overpartition of n with no parts divisible by 𝑙. Recently, the authors introduced a partition statistic called r𝑙-crank of 𝑙-regular overpartitions. Let Mr𝑙(m, n) denote the number of 𝑙-regular overpartitions of n with r𝑙-crank m. In this paper, we investigate the monotonicity property and the unimodality of Mr3(m, n). We prove that Mr3(m, n) ≥ Mr3(m, n - 1) for any integers m and n ≥ 6 and the sequence {Mr3(m, n)}|m|≤n is unimodal for all n ≥ 14.

A Study on Spatial-temporal indexing for querying current and past positions (현재와 과거 위치 질의를 위한 시공간 색인에 관한 연구)

  • Jun, Bong-Gi
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.8 no.6
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    • pp.1250-1256
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    • 2004
  • The movement of continuously changing positions should be stored and indexed for querying current and past positions. A simple extension of the original R-tree to add time as another dimension, called 3D R-tree, does not handle current position queries and does not address the problem of low space utilization due to high overlap of index nodes. In this paper, 1 propose the dynamic splitting policy for improving the 3D R-tree in order to improve space utilization of split nodes. 1 also extend the original 3D R-tree by introducing a new tagged index structure for being able to query the current and past positions of moving objects. 1 found out that my extension of the original R-tree, called the tagged dynamic 3DR-tree, outperforms both the 3D R-tree and 75-tree when querying current and past position.

R3V6 Amphiphilic Peptide with High Mobility Group Box 1A Domain as an Efficient Carrier for Gene Delivery

  • Ryu, Jaehwan;Jeon, Pureum;Lee, Minhyung
    • Bulletin of the Korean Chemical Society
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    • v.34 no.12
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    • pp.3665-3670
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    • 2013
  • The R3V6 peptide includes a hydrophilic arginine stretch and a hydrophobic valine stretch. In previous studies, the R3V6 peptide was evaluated as a gene carrier and was found to have low cytotoxicity. However, the transfection efficiency of R3V6 was lower than that of poly-L-lysine (PLL) in N2A neuroblastoma cells. In this study, the transfection efficiency of R3V6 was improved in combination with high mobility group box 1A domain (HMGA). HMGA is originated from the nuclear protein and has many positively-charged amino acids. Therefore, HMGA binds to DNA via charge interaction. In addition, HMGA has a nuclear localization signal peptide and may increase the delivery efficiency of DNA into the nucleus. The ternary complex with HMGA, R3V6, and DNA was prepared and evaluated as a gene carrier. First, the HMGA/DNA complex was prepared with a negative surface charge. Then, R3V6 was added to the complex to coat the negative charges of the HMGA/DNA complex, forming the ternary complex of HMGA, R3V6, and DNA. A physical characterization study showed that the ternary complex was more stable than the PLL/DNA complex. The HMGA/R3V6/DNA complex had a higher transfection efficiency than the PLL/DNA, HMGA/DNA, or R3V6/DNA complexes in N2A cells. Furthermore, the HMGA/R3V6/DNA complex was not toxic to cells. Therefore, the HMGA/R3V6/DNA complex may be a useful gene delivery carrier.

SAP R/3 System Interface Technologies Analysis and VBA Applied Data Input Proposal (SAP R/3 시스템 인터페이스 기술분석과 VBA 활용 데이터 입력 제안)

  • Kim, Yeong Real;Kang, Tae Gu;Lee, Eun Beom
    • Journal of Korea Society of Industrial Information Systems
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    • v.21 no.2
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    • pp.93-105
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    • 2016
  • Many companies have attempted to strengthen competitiveness by introducing information technology ERP system in a changing and global competitive business environment. However, ERP systems can not meet all the requirements that occur in the enterprise. After the system introduced, When it operates with existing systems or with new systems, the need for strong interface technology along with the appearance of the ERP have been gradually increased. In this paper, we selected SAP R/3 system as a typical ERP system. Among the modules of SAP R/3, MM module was chosen. Interface technologies for SAP R/3 Systems integrate mulltiple R/3 systems and non R/3 systems, and play important roles in expanding the company's business process. VBA approach was implemented as the data interface design between SAP R/3 and other systems. It proved to reduce the feeling of resistance from user's point of view. It also proved to improve the operational efficiency in a convenient input and output manner of purchase request data.

A Comparison of 3D R-tree and Octree to Index Large Point Clouds from a 3D Terrestrial Laser Scanner (대용량 3차원 지상 레이저 스캐닝 포인트 클라우드의 탐색을 위한 3D R-tree와 옥트리의 비교)

  • Han, Soo-Hee;Lee, Seong-Joo;Kim, Sang-Pil;Kim, Chang-Jae;Heo, Joon;Lee, Hee-Bum
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.29 no.1
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    • pp.39-46
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    • 2011
  • The present study introduces a comparison between 3D R-tree and octree which are noticeable candidates to index large point clouds gathered from a 3D terrestrial laser scanner. A query method, which is to find neighboring points within given distances, was devised for the comparison, and time lapses for the query along with memory usages were checked. From tests conducted on point clouds scanned from a building and a stone pagoda, it was shown that octree has the advantage of fast generation and query while 3D R-tree is more memory-efficient. Both index and leaf capacity were revealed to be ruling factors to get the best performance of 3D R-tree, while the number of level was of oetree.

THE RANGE OF r-MAXIMUM INDEX OF GRAPHS

  • Choi, Jeong-Ok
    • Bulletin of the Korean Mathematical Society
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    • v.55 no.5
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    • pp.1397-1404
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    • 2018
  • For a connected graph G, an r-maximum edge-coloring is an edge-coloring f defined on E(G) such that at every vertex v with $d_G(v){\geq}r$ exactly r incident edges to v receive the maximum color. The r-maximum index $x^{\prime}_r(G)$ is the least number of required colors to have an r-maximum edge coloring of G. In this paper, we show how the r-maximum index is affected by adding an edge or a vertex. As a main result, we show that for each $r{\geq}3$ the r-maximum index function over the graphs admitting an r-maximum edge-coloring is unbounded and the range is the set of natural numbers. In other words, for each $r{\geq}3$ and $k{\geq}1$ there is a family of graphs G(r, k) with $x^{\prime}_r(G(r,k))=k$. Also, we construct a family of graphs not admitting an r-maximum edge-coloring with arbitrary maximum degrees: for any fixed $r{\geq}3$, there is an infinite family of graphs ${\mathcal{F}}_r=\{G_k:k{\geq}r+1\}$, where for each $k{\geq}r+1$ there is no r-maximum edge-coloring of $G_k$ and ${\Delta}(G_k)=k$.

Physical Mapping of rDNAs Using McFISH in Anemarrhena asphodeloides Bunge (지모에서 McFISH를 이용한 rDNAs의 물리지도 작성)

  • Kim, Soo-Young;Choi, Hae-Woon;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.6
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    • pp.515-518
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    • 2004
  • Anemarrhena asphodeloides, a medicinal plant, has chromosome number of 2n=2x=22. To characterize the somatic metaphase chromosomes, physical mapping of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) was applied. Two pairs of 45S rDNA loci were detected on the terminal regions of the short arm of chromosomes 1 and 3. A pair of 5S rDNA signal was observed on the short arm of chromosome 3. 5S rDNA site seemed to be the same locus as one of the 45S rDNA site. McFISH was very useful tool for the localization and identification of rDNAs on the metaphase chromosomes in A. asphodeloides.

Growth and Yield Responses of Soybean to Overhead Flooding Duration at Four Growth Stages (관수시간에 따른 콩의 생육 및 수량반응)

  • 박경열;이종형;조영철
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.1
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    • pp.92-97
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    • 1995
  • The objective of this study is to investigate the growth characters of overhead flooded soybean plants at four growth stage. Overhead flooding treatments were applied at the vegetative growth stage ($V_3,\;V_6$) and the reproductive stage ($R_2,\;R_4$) for 6.12.24 hrs, respectively. Yield and yield components were more decreased as the overhead flooding duration was longer and the growth stage was later. Yield was not reduced significantly in soybean plants flooded at $V_3$ stage regardless of flooding duration, and flooded 6 or 12 hrs at $V_6$ stage. When compared to the control, 27 to 36% of yield reduction was observed in soybean plants flooded for 24 hrs at $V_6$ stage, 6 or 12 hrs at $R_2$ stage, and 6 hrs at $R_4$ stage. And 43%, 53% and 66% of yield were reduced through the flooding treatment for 24 hrs at $R_2$ stage 12 hrs and 24 hrs at $R_4$ stage, respectively. So yield reduction was higher in overhead flooded soybean plants at the reproductive stage than that at the vegetative growth stage.

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