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Physical Mapping of rDNAs Using McFISH in Anemarrhena asphodeloides Bunge  

Kim, Soo-Young (School of Biosciences & Biotechnology, Chungnam Natl. Univ.)
Choi, Hae-Woon (School of Biosciences & Biotechnology, Chungnam Natl. Univ.)
Bang, Jae-Wook (School of Biosciences & Biotechnology, Chungnam Natl. Univ.)
Publication Information
Korean Journal of Medicinal Crop Science / v.12, no.6, 2004 , pp. 515-518 More about this Journal
Abstract
Anemarrhena asphodeloides, a medicinal plant, has chromosome number of 2n=2x=22. To characterize the somatic metaphase chromosomes, physical mapping of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) was applied. Two pairs of 45S rDNA loci were detected on the terminal regions of the short arm of chromosomes 1 and 3. A pair of 5S rDNA signal was observed on the short arm of chromosome 3. 5S rDNA site seemed to be the same locus as one of the 45S rDNA site. McFISH was very useful tool for the localization and identification of rDNAs on the metaphase chromosomes in A. asphodeloides.
Keywords
Anemarrhena asphodeloides; McFISH; physical mapping; rDNAs;
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Times Cited By KSCI : 1  (Citation Analysis)
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