• 생물환경조절학회지
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• 제19권1호
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• pp.36-48
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• 2010

본 연구에서는 자작나무, 개벚나무, 함박꽃나무를 대상으로 피음수준을 전광 처리구(상대투광율; 100%), 약피음 처리구(상대투광율; 64~73%), 보통피음 처리구 (상대투광율; 35~42%), 강피음 처리구(상대투광율; 9~16%)로 달리하여 이들의 내음성 및 광 요구도에 관한 광합성 특성과 엽록소 형광 반응, 엽록소 함량을 조사 분석하였다. 세 수종의 생육시기별 광합성 능력은 7월과 9월에 가장 높은 값을 나타냈으며, 자작나무와 개벚나무에 비해 함박꽃나무의 광합성 능력이 매우 낮았다. 순양자수율 또한 광합성 능력과 같은 경향을 나타냈다. 수목의내음성 수준을 판단할 수 있는 광보상점은 함박꽃나무가 자작나무와 개벚나무에 비해 강한 내음성을 나타냈다. 피음 처리별 광합성 능력에서는 자작나무는 전광 처리구에서 가장 우수하였으며, 개벚나무는 시기별 차이는 있지만 강피음 처리구를 제외한 나머지 세 처리구에서 비슷한 광합성 능력을 보였다. 함박꽃나무는 보 통피음 처리구에서 가장 좋은 광합성 능력을 나타냈다. 피음 처리별 엽록소 형광 반응 특성에서 광화학 반응에 대한 순양자수율의 최대치인 광화학효율($F_v/F_m$)은 자 작나무의 경우 전광 처리구에서 가장 우수하였으며 피음 수준이 증가할수록 감소하였다. 개벚나무는 약피음 처리구에서 가장 우수한 광화학효율을 보였으며, 함박꽃 나무는 보통피음 처리구에서 가장 양호한 광화학효율을 나타냈다. 특히 함박꽃나무는 다른 두 수종과는 달리 전광 처리구에서 가장 낮은 값을 나타냈다. 이 결과는 세 수종의 피음 수준별 광합성 능력과 같은 결과를 보였다. 실험대상 수종들의 총 엽록소 함량은 세 수종 모두 피음 수준이 증가할수록 높아지는 경향을 보였다. 특히 전광 처리구에 비해서 강피음 처리구에서 총 엽록소 함량이 유의적 차이를 보이면서 가장 높게 나타났다. 엽록소 a와 b 각각의 함량 변화도 총 엽록소 함량과 같은 경향이었다. 피음 수준이 높아지면서 엽록소 b의 함량 증가가 엽록소 a의 함량에 비해 상대적으로 더 크게 증가하였는데 이 때문에 피음 강도가 강해지면서 엽록소 a/b율이 감소하는 경향을 나타냈다. 위의 결과를 종합해 볼 때 세 수종의 적정 생육 광도는 자작나무는 전광 처리구(상대투광율; 100%), 개 벚나무는 약피음 처리구(상대투광율; 64~73%), 함박꽃 나무는 보통피음 처리구(상대투광율; 35~42%)가 적합 하다고 판단된다. 그러나 수목의 생육에 있어서 광도뿐만 아니라 온도, 습도, 토양환경, 경쟁식생 등 여러 가지 다양한 환경인자가 관여하기 때문에 광도 변화와 연계한 보다 많은 연구가 필요할 것으로 판단된다.

• Molecules and Cells
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• 제40권9호
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• pp.655-666
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• 2017

We constructed a large $na{\ddot{i}}ve$ human Fab library ($3{\times}10^{10}$ colonies) from the lymphocytes of 809 human donors, assessed available diversities of the heavy-chain variable (VH) and ${\kappa}$ light-chain variable (VK) domain repertoires, and validated the library by selecting Fabs against 10 therapeutically relevant antigens by phage display. We obtained a database of unique 7,373 VH and 41,804 VK sequences by 454 pyrosequencing, and analyzed the repertoires. The distribution of VH and VK subfamilies and germline genes in our antibody repertoires slightly differed from those in earlier published natural antibody libraries. The frequency of somatic hypermutations (SHMs) in heavy-chain complementarity determining region (HCDR)1 and HCDR2 are higher compared with the natural IgM repertoire. Analysis of position-specific SHMs in CDRs indicates that asparagine, threonine, arginine, aspartate and phenylalanine are the most frequent non-germline residues on the antibody-antigen interface and are converted mostly from the germline residues, which are highly represented in germline SHM hotspots. The amino acid composition and length-dependent changes in amino acid frequencies of HCDR3 are similar to those in previous reports, except that frequencies of aspartate and phenylalanine are a little higher in our repertoire. Taken together, the results show that this antibody library shares common features of natural antibody repertoires and also has unique features. The antibody library will be useful in the generation of human antibodies against diverse antigens, and the information about the diversity of natural antibody repertoires will be valuable in the future design of synthetic human antibody libraries with high functional diversity.

• Clinical and Experimental Pediatrics
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• 제53권10호
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• pp.898-908
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• 2010

Purpose: The neuroprotective effects of erythropoietin (EPO) have been recently shown in many animal models of brain injury, including hypoxic-ischemic (HI) encephalopathy, trauma, and excitotoxicity; however, limited data are available for such effects during the neonatal periods. Therefore, we investigated whether recombinant human EPO (rHuEPO) can protect against perinatal HI brain injury via an antiapoptotic mechanism. Methods: The left carotid artery was ligated in 7-day-old Sprague-Dawley (SD) rat pups ($in$ $vivo$ model). The animals were divided into 6 groups: normoxia control (NC), normoxia sham-operated (NS), hypoxia only (H), hypoxia+vehicle (HV), hypoxia+rHuEPO before a hypoxic insult (HE-B), and hypoxia+rHuEPO after a hypoxic insult (HE-A). Embryonic cortical neuronal cell culture of SD rats at 18 days gestation ($in$ $vitro$ model) was performed. The cultured cells were divided into 5 groups: normoxia (N), hypoxia (H), and 1, 10, and 100 IU/mL rHuEPO-treated groups. Results: In the $in$ $vivo$ model, Bcl-2 expressions in the H and HV groups were lower than those in the NC and NS groups, whereas those in the HE-A and HE-B groups were greater than those of the H and HV groups. The expressions of Bax and caspase-3 and the ratio of Bax/Bcl-2 were in contrast to those of Bcl-2. In the $in$ $vitro$ model, the patterns of Bcl-2, Bax, and caspase-3 expression and Bax/Bcl-2 ratio were similar to the results obtained in the in vivo model. Conclusion: rHuEPO exerts neuroprotective effect against perinatal HI brain injury via an antiapoptotic mechanism.

• 생약학회지
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• 제39권3호
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• pp.199-202
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• 2008

A high performance liquid chromatographic (HPLC) method for the simultaneous determination of hesperidin and glycyrrhizin was established for the quality control of traditional herbal medicinal preparation, Pyungwi-san (PWS). Separation and quantification were successfully achieved with a Waters XTerra RP18 column ($5{\mu}m$, 4.6 mm I.D. ${\times}$ 150 mm) by gradient elution of a mixture of acetonitrile and water containing 0.03% phosphoric acid (pH 2.03) at a flow rate of 1.0 ml/min. The diode-array UV/vis detector (DAD) was used for the detection and the wavelength for quantification was set at 230 nm. The presence of hesperidin and glycyrrhizin in this extract was ascertained by retention time, spiking with each authentic standard and UV spectrum. All four compounds showed good linearity $(r^2>0.995)$ in a relatively wide concentration ranges. The R.S.D. for intra-day and inter-day precision was less than 7.0% and the limits of detection (LOD) were less than 60 ng. The mean recovery of each compound was 99.0-105.6% with R.S.D. values less than 4.0%. This method was successfully applied to the determination of contents of hesperidin and glycyrrhizin in three commercial products of PWS. These results suggest that the developed HPLC method is simple, effective and could be readily utilized as a quality control method for commercial PWS products.

• The Journal of Korean Physical Therapy
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• 제13권3호
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• pp.585-596
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• 2001

The twenty one individuals with total knee replacement who were admitted to Kang-Dong Hospital for comprehensive physical therapy were studied in order to demonstrate the effectiveness of an isokinetic test program using the Cybex 6000 machine. The subjects were divided into three groups with the isometric exercise group receiving isometric exercise, the isokinetic eccentric exercise group, and isokinetic concentric exercise group receivind isokinetic exercise(eccentric, concentric) to knee flexors and extensors muscles for a six weeks' period using the Cybex 6000. The results are follow: 1. The extensors were increased significantly at all groups after 6 weeks training(p<.05). The flexors were increased significantly at isokinetic eccentric and isokinetic concentric group but no significantly differences at isometric group(p>.05) 2. At the effect of extensors and flexors after 6 weeks training. higher to 30$^{\circ}$ /sec of isokinetic concentric exercise, lower to 120$^{\circ}$ /sec of isometric exercise. 3. The peak torque was more increased significantly in the flexors and extensors of the isokinetic conccentric exercise among three groups. 4. The total work was more increased significantly in the flexors and extensors of the isokinetic conccentric exercise among three groups. 5. The ratio of peak torque to body weight were more increased significantly in the flexors and extensors of the isikinetic conccentric exercise among three groups. 6. The average power was more increased significantly in the flexors and extensors of the isikinetic conccentric exercise among three groups. 7. The average R.O.M in the pre-exercise and post-exercise was not different significantly in all three groups. According to the above results, In the muscle strength recovery for total knee replacement patients, isokinetic concentric exercise group was significantly greater than the isokinetic eccentric and isometric exercise groups after a six weeks training.

• 대한핵의학회지
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• 제31권4호
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• pp.427-432
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• 1997

Re-188은 반감기가 17시간이고 진단을 위한 영상화가 가능한 감마선(155keV)을 방출하며 치료용으로 적당한 베타선(2.12MeV)을 동시에 방출하여 혈관성형술 풍선에 넣어 조사하는 접촉 베타선 방출체 치료용 방사성핵종으로 유력하다. 이 연구에서는 Re-188-DTPA를 관상동맥풍선 성형술시 풍선에 주입하는 방사성 동위원소로 쓸 수 있을지 알기 위해 우선 표지법과 풍선에서 혈관내로 샜을 때의 생체내 분포를 조사하였다. Re-188과 DTPA를 표지하는 방법을 확립하였고 표지효율은 95%, 실온과 사람 혈청에서 안정하였다. 마우스의 체내분포와 랫트와 실험견에서 얻은 신장 시간방사능곡선이 Re-188-DTPA가 신장을 통해 빠르게 제거된다는 것을 밝혔다. 이러한 결과는 Re 188-DTPA를 관상동맥의 재협착을 방지하기 위해 관상동맥풍선 성형술시 풍선에 주입하는 방사성 동위원소로 사용하여도 좋음을 시사한다.

• 대한화장품학회지
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• 제32권1호
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• pp.7-15
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• 2006

본 연구에서는 발아 검은 쌀로부터 분리한 올리고펩타이드($Oligosproutin^{(R)}$)의 피부 탄력 개선 효과에 대하여 연구하였다. 올리고펩타이드가 각질형성세포의 유전자 발현에 미치는 영향을 알아보고자 DNA microarray를 수행하였다. 각질형성세포에 올리고펩타이드를 처리하여 유전자의 발현이 2배 이상 증가하는 유전자는 745개이고, 2배 이상 감소하는 유전자는 1011개로 나타났다. 이 중 hyaluronan synthase 2 (HAS2)의 유전자 발현이 2배 이상 증가함을 확인하였다. 올리고펩타이드를 처리한 각질형성세포에서 세포활성 증가, HAS2 유전자 발현 증가, 세포내 항산화 효과를 확인하였다. 올리고펩타이드를 함유한 O/W 에멀젼을 이용한 임상실험에서 피부의 탄력 개선 효과를 확인하였다. 본 연구를 통하여 발아 검은 쌀로부터 분리한 올리고펩타이드는 피부 탄력 개선을 주는 항노화 화장품 개발에 응용될 수 있을 것이라 기대된다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.241-241
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• 2017

Sweet potatoes (Ipomoea batatas (L.) LAMK.,) have been cultivated in Central and South America for about 2000 years and are now grown mainly in Asia and South America. Sweet potatoes are annual in the temperate region, but are classified as perennial in the tropical region. In 2000, the cultivation area of sweet potatoes decreased to about 16,000 ha in 2000, but the cultivation area increased slightly in recent 20,000 ha in Korea. Sweet potatoes do not show higher maximum dry matter production of 120 ~ 150g per plant, and the leaf area index (LAI), which maximizes dry matter production, is known as 3.0 ~ 4.0. As the leaf area increase, the penetration of light into the canopy becomes poor, and sufficient photosynthesis cannot be achieved in the lower leaves, on the other hand the respiration increase, which results in poor dry matter production. This study was conducted to know the responses of sweet potatoes to intensive shading treatment of 80% shading. This experiment was conducted for about 42 days from September 6, 2016 to October 18, 2016 at Gyeongsang National University Experimental Farm, Jinju, Korea. The plant canopy was shaded with black nylon 80% shade cloth suspended 1.2 m above the ground. The photosynthetic rate, stomatal conductance, chlorophyll fluorescence, SPAD and NDVI were measured in 3 replicates every 7 days after shading initiation. After the fresh weight was measured, the samples were dried at $80^{\circ}C$ in a dry oven and measured. By the 80% shading treatment, chlorophyll fluorescence of the treated plants was slightly higher than that of the control, the SPAD value was higher by 3.4 and NDVI value was higher by 0.01. However, photosynthetic rate and stomatal conductance were lower than those of the control. The stomatal conductance of the control were two times higher than those of the control and the photosynthetic rate of the control was four times higher than that of the control. In control, plant showed a tendency to steadily increase in fresh weight and dry weight. However, in the case of shading treatment, the tendency to increase in the fresh and dry weight of tuberous roots was not clear. The fresh weight of shoot showed a tendency to increase steadily while the difference between treatment and control was not large, but tended to decrease after frost.

• 대한수의학회지
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• 제38권3호
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• pp.474-487
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• 1998

The present study was undertaken to investigate the morphological characteristics of trigeminal nerve in the Korean native goat by macroscopic methods. Trigeminal nerve was originated from the lateral side of pons, and extended shortly forward to form trigeminal ganglion at the opening of oval foramen. Thereafter this nerve was divided into maxillary, mandibular and ophthalmic nerve. Ophthalmic nerve gave off the zygomaticotemporal branch, frontal nerve, frontal sinus branch, and was continued as the nasociliary nerve. Maxillary nerve gave rise to the zygomaticofacial branch, accessory zygomaticofacial branch, communicating branch with oculomotor nerve, pterygopalatine nerve, caudal superior alveolar branch, malar branch and was continued as the infraorbital nerve. Mandibular nerve was divided into the masseteric nerve, buccal nerve, lateral pterygoid nerve, medial pterygoid nerve, nerve to tensor tympani m., auriculotemporal nerve, and furnished the inferior alveolar nerve and lingual nerve as terminal branches. The course and distribution of the trigeminal nerve in the Korean native goat appeared to be similar to that in other small ruminants such as sheep and goat. But the main differences from other small ruminants were as follows : 1. There was no accessory branch of the major palatine nerve. 2. The caudal superior alveolar branch was directly branched from the maxillary nerve. 3. The communicating branch with oculomotor nerve was originated from maxillary nerve or common trunk with zygomaticofacial branch. 4. The malar branch arose from the maxillary nerve at the rostral to the origin of the caudal superior alveolar branch. 5. The inferior alveolar nerve originated in a common trunk with the lingual nerve. 6. The mylohyoid nerve arose at the origin of the inferior alveolar nerve. 7. The zygomaticotemporal branch was single fascicle, and gave off lacrimal nerve and cornual branch. 8. The base of horn was provided by the cornual branches of zygomaticotemporal branch and infratrochlear nerve of nasociliary nerve.

• 대한전자공학회논문지SD
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• 제45권10호
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• pp.7-14
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• 2008

본 논문에서는 $1.0{\mu}m$ BCD 650V 공정을 이용하여 향상된 잡음 내성과 높은 전류 구동 능력을 갖는 고전압 구동 IC를 설계하였다. 설계된 고전압 구동 IC는 500kHz의 고속 동작이 가능하고, 입력 전압의 범위가 최대 650V이다. 설계된 IC에 내장된 상단 레벨 쉬프터는 잡음 보호회로와 슈미트 트리거를 포함하고 있으며 최대 50V/ns의 높은 dv/dt 잡음 내성을 가지고 있다. 또한 설계된 숏-펄스 생성회로가 있는 상단 레벨 쉬프터의 전력 소모는 기존 회로 대비 40% 이상 감소하였다. 이외에도 상 하단 파워 스위치의 동시 도통을 방지하는 보호회로와 구동부의 전원 전압을 감지하는 UVLO(Under Voltage Lock-Out) 회로를 내장하여 시스템의 안정도를 향상시켰다. 설계된 고전압 구동 IC의 특성 검증에는 Cadence사의 spectre 및 PSpice를 이용하였다.