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Simultaneous Determination of Hesperidin and Glycyrrhizin in Pyungwi-san by HPLC/DAD  

Lee, Mi-Kyeong (Institute for Life Science, Elcom Science Co. Ltd.)
Choe, Ok-Gyeong (Institute for Life Science, Elcom Science Co. Ltd.)
Park, Jin-Ho (Institute for Life Science, Elcom Science Co. Ltd.)
Cho, Jung-Hee (Herbal Medicine Evaluation Department, Korea Drug & Food Administration)
Kim, Do-Hoon (Herbal Medicine Evaluation Department, Korea Drug & Food Administration)
Baek, Ju-Hyun (Herbal Medicine Evaluation Department, Korea Drug & Food Administration)
Kim, Hyo-Jin (College of Pharmacy, Dongduk Women's University)
Lee, Ki-Yong (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Kim, Sang-Du (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Kim, Young-Choong (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Sung, Sang-Hyun (College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University)
Publication Information
Korean Journal of Pharmacognosy / v.39, no.3, 2008 , pp. 199-202 More about this Journal
Abstract
A high performance liquid chromatographic (HPLC) method for the simultaneous determination of hesperidin and glycyrrhizin was established for the quality control of traditional herbal medicinal preparation, Pyungwi-san (PWS). Separation and quantification were successfully achieved with a Waters XTerra RP18 column ($5{\mu}m$, 4.6 mm I.D. ${\times}$ 150 mm) by gradient elution of a mixture of acetonitrile and water containing 0.03% phosphoric acid (pH 2.03) at a flow rate of 1.0 ml/min. The diode-array UV/vis detector (DAD) was used for the detection and the wavelength for quantification was set at 230 nm. The presence of hesperidin and glycyrrhizin in this extract was ascertained by retention time, spiking with each authentic standard and UV spectrum. All four compounds showed good linearity $(r^2>0.995)$ in a relatively wide concentration ranges. The R.S.D. for intra-day and inter-day precision was less than 7.0% and the limits of detection (LOD) were less than 60 ng. The mean recovery of each compound was 99.0-105.6% with R.S.D. values less than 4.0%. This method was successfully applied to the determination of contents of hesperidin and glycyrrhizin in three commercial products of PWS. These results suggest that the developed HPLC method is simple, effective and could be readily utilized as a quality control method for commercial PWS products.
Keywords
Pyungwi-san (PWS); HPLC; quantification; validation;
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1 Bennett, J.O., Yu, O., Heatherly, L.G. and Krishnan, H.B.(2004) Accumulation of genistein and daidzein, soybean isoflavones implicated in promoting human health, is significantly elevated by irrigation. J. Agric. Food Chem. 52: 7574-7579   DOI   ScienceOn
2 Xue, T.H. and Roy, R. (2003) Studying Traditional Chinese Medicine. Science 300: 740-741   DOI   ScienceOn
3 Normile, D. (2003) Asian medicine. The new face of traditional Chinese medicine. Science 299: 188-190   DOI   ScienceOn
4 Jiang, W.Y. (2005) Therapeutic wisdom in traditional Chinese medicine: a perspective from modern science. Trends Pharmacol. Sci. 26: 558-563   DOI   ScienceOn
5 Anttonen, M.J., Hoppula, K.I., Nestby, R., Verheul, M.J. and Karjalainen, R.O. (2006) Influence of fertilization, mulch color, early forcing, fruit order, planting date, shading, growing Environment, and genotype on the contents of selected phenolics in strawberry (Fragaria x ananassa Duch.) fruits. J. Agric. Food Chem. 54: 2614-2620   DOI   ScienceOn
6 Wang, S.Y., Zheng, W. and Galletta, G.J. (2002) Cultural system affects fruit quality and antioxidant capacity in strawberries. J. Agric. Food Chem. 50: 6534-6542   DOI   ScienceOn
7 Escarpa, A. and Gonzalez, M.C. (1999) Fast separation of (poly)phenolic compounds from apples and pears by high-performance liquid chromatography with diode-array detection. J. Chromatogr. A. 830: 301-309   DOI   ScienceOn
8 Lund, S.T. and Bohlmann, J. (2006) The molecular basis for wine grape quality-a volatile subject. Science 311: 804-805   DOI   ScienceOn
9 Lee, Y.-C., Huang, C.-Y., Wen, K.-C. and Suen, T.-T. (1995) Determination of liquiritin, glycyrrhizin, hesperidin, cinnamic acid, cinnamaldehyde, honokiol and magnolol in the traditional Chinese medicinal preparation Wuu-Ji-San by high-performance liquid chromatography. J. Chromatogr. A. 692: 137-145   DOI   ScienceOn