• Title/Summary/Keyword: 17B

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The Conversion of Lithocholic Acid into 5$\beta$-Androstan-3, 17-dione in the Cell-free System of Mycobacterium sp. NRRL B-3805

  • Lee, Kang-Man;Park, Hye-Kyung
    • Archives of Pharmacal Research
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    • v.14 no.3
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    • pp.261-265
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    • 1991
  • In a microbial cell-free extract system, side chain cleavage on various sterols and steroids was tested. The cell-free extracts of Mycobacterium sp. NRRL B-3805 showed the side chain cleavage activity on lithocholic acid to form 5$\beta$-androstan-3.17-dione. The properties of the activity were examined.

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The synthesis and crystal structure of $[({\eta}^5-Cp)Co]_2$(1,2-S,S-o-Carborane) $(C_{12}H_{20}B_{10}S_2Co_2)$ ($[({\eta}^5-Cp)Co]_2$ (1,2-S,S-o-Carborane) $(C_{12}H_{20}B_{10}S_2Co_2)$의 합성 및 결정구조)

  • Cho, Sung-Il
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.18 no.2
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    • pp.62-67
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    • 2008
  • An Organometallic compound, $C_{12}H_{20}B_{10}S_2Co_2$, was synthesized from o-carborane, $Cp^*Co(S_2B_2B_{10}H_{10})$ and $BH_3{\cdot}THF$. The molecular structure of this complex has been determined by X-ray diffraction. Crystallographic data: monoclinic, space group Cc, a=15.981(4) ${\AA}$, b=15.478(17) ${\AA}$, c=12.0562(17) ${\AA}$, ${\beta}=115.063(16)^{\circ}$, Z=4, V=9683(4) ${\AA}^3$. The structure was solved by direct methods and refined by full-matrix leat-squares methods to give a model with a reliability factor R = 0.0630 for 9948 reflections.

Design and Fabrication of a Weathercock-Shaped Double Bandwidth Microstrip Patch Antenna that Combines U-slot and Short-pin for WLAN Systems Systems (WLAN System을 위한 U-slot 및 Short-pin 결합한 바람개비 모양의 이중대역(5.2/5.8GHz) 마이크로스트립 패치 안테나 설계 및 제작)

  • Kim, Soon-Seob;Choi, Young-June;Joo, Young-Dal;Jung, Yoong-Joo
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.38B no.5
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    • pp.337-343
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    • 2013
  • In this paper, IEEE 802.11 based WLAN(5.2/5.8GHz) wideband Weathercock-shaped microstrip patch antenna was designed and manufactured. The antenna has a size of $17.4{\times}17.4mm^2$ and utilized FR-4 board. The size was minimized for mobility, and Weathercock-shaped U-slot and short-pin was inserted to satisfy adequate bandwidth and double bandwidth resonance characteristics. In addition, the antenna incorporated single both-sided patch, and simulation design optimized the Weathercock-shaped, position of the U-slot and the short-pin, and the length of the patch for the measurement. The manufactured antenna achieved a bandwidth of 695MHz from 5.2~5.8GHz zone(Return loss<-10dB). Achieved a beam width of $81.13^{\circ}$ and $85.43^{\circ}$ for 3-dB beam width of H plane and E p;ane radiation pattern, there was 3.17~4.85dBi gain.

Characterization of the recombinant cellulase B from Thermotoga maritima (Thermotoga maritima 유래 내열성 cellulase B 융합단백질의 특성 규명)

  • Chung Ho Kim
    • Journal of Applied Biological Chemistry
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    • v.65 no.4
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    • pp.383-386
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    • 2022
  • A gene encoding thermostable cellulase B (TmCelB) was isolated from Thermotoga maritima. The open reading frame (ORF) of TmCelB gene was 825bp long which predicted to encode 274 amino acid residues with a molecular weight of 31,732 Da. The 17 amino acid residues from N-terminal of the TmCelB was known as signal peptides. To analyze the enzymatic activity and biochemical properties, the ORF of TmCelB gene excluding a putative signal sequence encoding 17 amino acids were introduced into the E. coli expression vector, pRSET-B, and overexpressed in E. coli BL21. The optimum temperature of recombinant TmCelB was around 95 ℃, and the optimum pH of recombinant TmCelB was around pH 4.5. The recombinant TmCelB was stable at temperature below 100 ℃.

Full-Custom Design of a Compact 17x-17b Multiplier and its Efficient Test Methodology (풀커스텀(full-custom)방식의 17x-17b 곱셈기의 설계와 효율적인 테스트)

  • 문상국;문병인;이용석
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.26 no.3B
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    • pp.362-368
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    • 2001
  • 본 논문에서는 두 개의 17비트 오퍼랜드를 radix-4 Booths 알고리즘을 이용하여 곱셈 연산을 수행하는 곱셈기를 설계하고 효율적인 풀커스팀 디자인에 대한 테스트 방법을 제안하였다. 클럭 속도를 빠르게 하기 위하여 2단파이프라인 구조로 설계하고 규칙적인 레이아웃을 위해 4:2 CSA(Carry Save Adder)를 사용하였다. 회로는 LG 반도체의 0.6-um 3-Metal N-well CMOS 공정을 사용하여 칩으로 제작되었다. 새로운 개념의 모듈레벨 고착 고장 모델을 제안하였고 제안한 테스트 방법을 사용하여 관찰해야 하는 노드의 수를 약 88% 줄여 효율적인 고장 시뮬레이션을 수행하였다. 설계된 곱셈기는 9115개의 트랜지스터로 구성되며 코어 부분의 레이아웃 면적은 약 1135*1545 um2 이다. 제작된 칩은 전원접압 5V에서 약 24MHz의 클럭 주파수로 동작한다.

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Identification Based on Computational Analysis of rpoB Sequence of Bacillus anthracis and Closely Related Species (Bacillus anthracis와 그 유연종의 rpoB 유전자 컴퓨터 분석을 통한 동정)

  • Kim, Kyu-Kwang;Kim, Han-Bok
    • Korean Journal of Microbiology
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    • v.44 no.4
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    • pp.333-338
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    • 2008
  • Computational analysis of partial rpoB gene sequence (777 bp) was done in this study to identify B. anthracis and its closely related species B. cereus and B. thuringiensis. Sequence data including 17 B. anthracis strains, 9 B. cereus strains, and 7 B. thuringiensis strains were obtained by searching databases. Those sequences were aligned and used for other computational analysis. B. anthracis strains were identificated by in silico restriction enzyme digestion. B. cereus and B. thuringiensis were not segregated by this method. Those sequencing and BLAST search were required to distinguish the two. In actual identification tests, B. anthracis strains could be identified by PCR-RFLP, and B. cereus and B. thuringiensis strains were distinguished by BLAST search with reliable e-value. In this study fast and accurate method for identifying three Bacillus species, and flow chart of identification were developed.

Inhibition of Bacillus cereus in Cheonggukjang Fermented with Bacillus Starters with Antimicrobial Activities (항균력을 지닌 Bacillus 균주들을 종균으로 사용한 청국장에서 Bacillus cereus 억제)

  • Lee, Jae Yong;Shim, Jae Min;Liu, Xiaoming;Yao, Zhuang;Lee, Kang Wook;Cho, Kye Man;Kim, Gyoung Min;Shin, Jung-Hye;Kim, Jong-Sang;Kim, Jeong Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.5
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    • pp.736-745
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    • 2016
  • Cheonggukjang, a traditional Korean fermented soy food, was prepared by inoculation of Bacillus subtilis EMD4 or Bacillus amyloliquefaciens EMD17 with anti-bacterial or anti-fungal activities into soybeans. Cheonggukjang was also prepared by co-inoculation of EMD4 and EMD17 (1:1, v/v). Control cheonggukjang was prepared by using B. subtilis KACC16450 (Natto strain). Growth of B. cereus cells spiked with starter organisms was completely inhibited by B. amyloliquefaciens EMD17 after 12 h of fermentation at $37^{\circ}C$. Growth of B. cereus was also inhibited by B. subtilis EMD4, but the degree of inhibition was weaker. After 48 h of fermentation, cheonggukjang samples were stored for 10 days at $4^{\circ}C$. B. cereus cells were not detected from cheonggukjang inoculated with EMD4, whereas significant numbers still present in control. The pH values of cheonggukjang samples were not significantly different. During fermentation, cheonggukjang fermented with EMD17 showed the highest fibrinolytic activity and during storage, cheonggukjang fermented with a Natto strain was the highest. Cheonggukjang fermented with a Natto strain also showed the highest amount of total phenolic compounds. The results show that control of B. cereus in cheonggukjang is possible by using starters such as B. amyloliquefaciens EMD17.

Development of Combinational 2-Way Isohybrid for GSM1900 Base Station (GSM1900 기지국용 일체형 2-Way Isohybrid의 개발)

  • Park, Noh-Joon;Jung, Seung-Woo;Kang, Young-Jin;Choi, U-Sung
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.11 no.8
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    • pp.1415-1423
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    • 2007
  • Total efficiency of transmitter system was improved by developing combinational 2-Way Isohybrid for GSM1900 base station in this paper. The size of product was decreased and the characteristics were stabilized. The isohybrid transmitter combiner of chip coupler type and hybrid coupler type were designed and fabricated. Insertion loss for chip coupler type isohybrid was $3.17\;dB{\sim}3.47\;dB$. On the other hand, insertion loss for hybrid coupler type was $3.17\;dB{\sim}3.42\;dB$. Isolations between ANT and port were $24.23\;dB{\sim}29.98\;dB$ in case of chip coupler type Isohybrid and were $26.28\;dB{\sim}36.91\;dB$ in case of hybrid coupler type, respectively.

Design of 900 MHz CMOS Low Noie Amplifier (900 MHz CMOS 저잡음 증폭기의 설계)

  • 윤상영;윤헌일;정용채;정항근;황인갑
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.11 no.6
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    • pp.893-899
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    • 2000
  • A 900 MHz low-noise amplifier(LNA) with a measured noise figure of 4.8 dB and an associated gain of 13.2 dB was fabricated in a 0.65 $\mu$m CMOS. The inductive source architecture of offers the possibility of achieving the best noise performance. At 900 MHz, the fabricated LNA dissipates 39 mW from a single 3 V power supply including the bias circuitry and provides -26dB input return loss, -17 dB output return loss, and an input 1-dB compression level of -12 dBm.

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