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Identification Based on Computational Analysis of rpoB Sequence of Bacillus anthracis and Closely Related Species  

Kim, Kyu-Kwang (Daeil Foreign High School)
Kim, Han-Bok (Department of Biotechnology, The Research Institute for Basic Science, Hoseo University)
Publication Information
Korean Journal of Microbiology / v.44, no.4, 2008 , pp. 333-338 More about this Journal
Abstract
Computational analysis of partial rpoB gene sequence (777 bp) was done in this study to identify B. anthracis and its closely related species B. cereus and B. thuringiensis. Sequence data including 17 B. anthracis strains, 9 B. cereus strains, and 7 B. thuringiensis strains were obtained by searching databases. Those sequences were aligned and used for other computational analysis. B. anthracis strains were identificated by in silico restriction enzyme digestion. B. cereus and B. thuringiensis were not segregated by this method. Those sequencing and BLAST search were required to distinguish the two. In actual identification tests, B. anthracis strains could be identified by PCR-RFLP, and B. cereus and B. thuringiensis strains were distinguished by BLAST search with reliable e-value. In this study fast and accurate method for identifying three Bacillus species, and flow chart of identification were developed.
Keywords
B. anthracis; B. cereus; B. thuringiensis; identification; rpoB;
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1 Drancourt, M. and D. Raoult. 2002. rpoB gene sequence-based identification of Staphylococcus species. J. Clin. Microbiol. 40, 1333-1338   DOI
2 Renesto, P., J. Gouvernet, M. Drancourt, V. Roux, and D. Raoult. 2001. Use of rpoB gene analysis for detection and identification of Bartonella species. J. Clin. Microbiol. 39, 430-437   DOI   ScienceOn
3 Mollet, C., M. Drancourt, and D. Raoult. 1997. rpoB sequence analysis as a novel basis for bacterial identification. Mol. Microbiol. 26, 1005-1011   DOI   ScienceOn
4 Korea Society for Medical Microbioloy. 2004. Medical Microbiology. 3rd ed.
5 Lee, S.H., B.J. Kim, J.H. Kim, K.H. Park, S.J. Kim, and Y.H. Kook. 2000. Differentiation of Borrelia burgdorferi sensu lato on the basis of RNA polymerase gene (rpoB) sequences. J. Clin. Microbiol. 38, 2557-2562   PUBMED
6 Ash, C., J.A.E. Farrow, M. Dorsch, E. Stackebrandt, and M.D. Collins. 1991. Comparative analysis of Bacillus anthracis, Bacillus cereus, and related species on the basis of reverse transcriptase sequencing of 16S rRNA. Int. J. Syst. Bacteriol. 41, 343-346   DOI   PUBMED   ScienceOn
7 Sabelnikov, A.G. and L.V. Ulyashova. 1990. Plasmid transformation of Bacillus cereus on cellophane membrane. FEMS Microbiol. Lett. 72, 123-126   DOI   ScienceOn
8 Zhong, W., Y. Shou, T.M. Yoshida, and B.L. Marrone. 2007. Differentiation of Bacillus anthracis, B. cereus, and B. thuringiensis by using pulsed-field gel electrophoresis. Appl. Environ. Microbiol. 73, 3446-3449   DOI   ScienceOn
9 Qi, Y., G. Patra, X. Liang, L.E. Williams, S. Rose, R.J. Redkar, and V.G. Del Vecchio. 2001. Utilization of the rpoB gene as a specific chromosomal marker for real-time PCR detection of Bacillus anthracis. Appl. Environ. Microbiol. 67, 3720-3727   DOI   ScienceOn
10 Ko, K.S., J.M. Kim, B.Y. Jung, W. Kim, I.J. Kim, and Y.H. Kook. 2003. Identification of Bacillus anthracis by sequence analysis and multiplex PCR. J. Clin. Microbiol. 41, 2908-2914   DOI
11 Wicks, A., N. Jayaswal, D. Lereclus, and L. Andrup. 1998. Characterization of plasmid pAW63, a second self-transformissible plasmid in Bacillus thuringiensis subsp. kurstaki HD73. Microbiology 144, 1263-1270   DOI   ScienceOn
12 Ingleby, T.V., T. O'Toole, D.A. Henderson, J.G. Barlett, M.S. Ascher, E. Eitzen, A.M. Friedlander, J. Gergerding, J. Hauer, J. McDade, M.T. Osterholm, G. Parker, T.M. Perl, P.K. Russel, and K. Tonat. 2002. Anthrax as a biological weapon, 2002. JAMA 287, 2236-2252   DOI   ScienceOn
13 Turnbull, P.C., R.A. Huston, M.J. Ward, M.N. Jones, C.P. Quinn, N.J. Finnie, C.J. Duggleby, J.M. Kramer, and J. Melling. 1992. Bacillus anthracis but not always anthrax. J. Appl. Bacteriol. 72, 21-28   PUBMED
14 Drancourt, M., A. Carlioz, and D. Raoult. 2001. rpoB sequence analysis of cultured Tropheryma whippelii. J. Clin. Microbiol. 39, 2425-2430   DOI   ScienceOn
15 Rasko, D.A., M.R. Altherr, C.S. Han, and J. Ravel. 2005. Genomics of the Bacillus cereus group of organisms. FEMS Microbiol. Rev. 29, 303-329   DOI   ScienceOn