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http://dx.doi.org/10.3839/jabc.2022.049

Characterization of the recombinant cellulase B from Thermotoga maritima  

Chung Ho Kim (Department of Food and Nutrition, Seowon University)
Publication Information
Journal of Applied Biological Chemistry / v.65, no.4, 2022 , pp. 383-386 More about this Journal
Abstract
A gene encoding thermostable cellulase B (TmCelB) was isolated from Thermotoga maritima. The open reading frame (ORF) of TmCelB gene was 825bp long which predicted to encode 274 amino acid residues with a molecular weight of 31,732 Da. The 17 amino acid residues from N-terminal of the TmCelB was known as signal peptides. To analyze the enzymatic activity and biochemical properties, the ORF of TmCelB gene excluding a putative signal sequence encoding 17 amino acids were introduced into the E. coli expression vector, pRSET-B, and overexpressed in E. coli BL21. The optimum temperature of recombinant TmCelB was around 95 ℃, and the optimum pH of recombinant TmCelB was around pH 4.5. The recombinant TmCelB was stable at temperature below 100 ℃.
Keywords
Cellulase; Cellulose; Thermostable; Thermotoga maritima;
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