Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Identification Based on Computational Analysis of rpoB Sequence of Bacillus anthracis and Closely Related Species

Bacillus anthracis와 그 유연종의 rpoB 유전자 컴퓨터 분석을 통한 동정

  • Kim, Kyu-Kwang (Daeil Foreign High School) ;
  • Kim, Han-Bok (Department of Biotechnology, The Research Institute for Basic Science, Hoseo University)
  • 김규광 (대일외국어고등학교) ;
  • 김한복 (호서대학교 자연과학대 생명공학과.호서대 기초과학연구소)
  • Published : 2008.12.31
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Abstract

Computational analysis of partial rpoB gene sequence (777 bp) was done in this study to identify B. anthracis and its closely related species B. cereus and B. thuringiensis. Sequence data including 17 B. anthracis strains, 9 B. cereus strains, and 7 B. thuringiensis strains were obtained by searching databases. Those sequences were aligned and used for other computational analysis. B. anthracis strains were identificated by in silico restriction enzyme digestion. B. cereus and B. thuringiensis were not segregated by this method. Those sequencing and BLAST search were required to distinguish the two. In actual identification tests, B. anthracis strains could be identified by PCR-RFLP, and B. cereus and B. thuringiensis strains were distinguished by BLAST search with reliable e-value. In this study fast and accurate method for identifying three Bacillus species, and flow chart of identification were developed.

Bacillus anthracis, B. cereus, B. thuringiensis 를 분류하기 위해 rpoB 유전자 배열을 이용한 컴퓨터 분석 작업을 수행하였다. 17개의 B. anthracis, 9개의 B. cereus, 7개의 B. thuringiensis 를 database에서 구하였다. B. anthracis 는 rpoB 유전자의 in silico 제한효소 절단에 의해, B. cereus, B. thuringiensis 2 group과 구별되었다. 그러나 B. cereus와 B. thuringiensis 는 제한효소 절단에 의해 구분되지는 않고, 염기배열과 Blast 탐색의 도움으로 구분이 가능하였다. 본 연구를 통해 3 종류의 Bacillus 종을 동정할 수 있는 알고리즘이 개발되었다.

Keywords

References

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