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Effects of Exogenous Ghrelin on the Behaviors and Performance of Weanling Piglets

  • Wu, Xingli;Tang, Maoyan;Ma, Qiugang;Hu, Xinxu;Ji, Cheng
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.6
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    • pp.861-867
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    • 2008
  • The objective of this study was to evaluate the effect of exogenous ghrelin on the behaviors, weight gain, and feed intakes of weanling piglets. A total of 25 pairs of Duroc$\times$Landrace$\times$Large White piglets weaned at 21 days of age were used in this experiment which finished on day 36. Each pair of healthy piglets from the same litter with similar body weight and of the same gender were selected and randomly arranged to ghrelin or control groups. Thus, there were 50 piglets (ghrelin 25; control 25) kept in 10 pens (ghrelin 5; control 5) and 5 piglets per pen. Initial body weight of the pigs did not differ between the control and ghrelin treatment ($7.43{\pm}0.17kg$; p = 0.81). Experimental pigs were infused with ghrelin ($1{\mu}g/d$ pig) via the marginal ear vein between 0750 and 0800 h at 22, 23, 24 days of age. Control pigs were infused with 0.9% saline. Feed consumption was measured on days 23, 24, 25, 29 and 36. Body weight was measured on days 22, 23, 24, 25, 29 and 36. Behavior data of individual piglets were collected by real-time observation from 0800-1500 h through remote supervisory equipment at 22, 23 and 24 days of age. The results indicated that ghrelin infusion could increase drinking (p<0.05) and lying behaviors (p<0.01) and decrease mounting behaviors (p<0.05). No significant influence of ghrelin was found on average daily weight gain (ADG) and average daily feed intake (ADFI) in this experiment (p>0.05). In conclusion, exogenous ghrelin by the method above and at the dosage of $1{\mu}g/d$ pig could cause a variety of behavioral effects, but not improve performance of weanling piglets.

Study on the Crystal Phases of $Ca_{1.5-1.5x}Bi_xVO_4$ Compositions by Bi Substitution (Bi 치환에 따른 $Ca_{1.5-1.5x}Bi_xVO_4$ 조성 화합물의 결정상에 관한 연구)

  • Kim, Myung Seab;Park, Sun Min;Kim, Ho Kun
    • Journal of the Korean Chemical Society
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    • v.43 no.5
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    • pp.547-551
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    • 1999
  • The phase change upon Bi substitutions in $Ca_{1.5-1.5x}Bi_xVO_4$ has been systematically studied. The x value corresponding to the maximum Bi substitutions reades to 0.14 and in this range($x{\leq}0.14$), the single phasic $Ca_3(VO_4)_2$ can be identified. However, a new phase of $BiV_{1.025}O_{4+x}$ is apparently formed along with the $Ca_3(VO_4)_2$ phase, when the x value exceeds beyond 0.18 ($x{\geq}0.18$). As a result of Bi substitution in the range of x$\leq$0.14, the interplanar space($d_300$) becomes larger as the Bi content increases. Since the composition of single phasic $Ca_{1.29}Bi_{0.14}VO_4$ (x=O.14) is, however, incongruent melting one, no definite melting point could be observed. But we found that its solidus temperature was 1182$^{\circ}C$ by DTA analysis.

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Analysis of Rhizosphere Soil Bacterial Communities on Seonginbong, Ulleungdo Island (울릉도 성인봉의 근권 토양 세균군집 분석)

  • Nam, Yoon-Jong;Yoon, Hyeokjun;Kim, Hyun;Kim, Jong-Guk
    • Journal of Life Science
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    • v.25 no.3
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    • pp.323-328
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    • 2015
  • The study of microbial diversity and richness in soil samples from a volcanic island named Ulleungdo, located east of South Korea. The soil bacterial communities on the Ulleungdo were analyzed using pyrosequencing method based on 16S rRNA gene. There were 1,613 operational taxonomic units (OUT) form soil sample. From results of a BLASTN search against the EzTaxon-e database, the validated reads (obtained after sequence preprocessing) were almost all classified at the phylum level. Proteobacteria was the most dominant phylum with 48.28%, followed by acidobacteria (26.30%), actionbacteria (6.89%), Chloroflexi (4.58), Planctomycetes (4.56%), Nitrospirae (1.83%), Bacteroidetes (1.51%), Verrucomicrobia (1.48%), and Gemmatimonadetes (1.11%). α-proteobacteria was the most dominant class with 36.07% followed by Acidobacteria_c (10.65%), Solibacteres (10.64%), δ-proteobacteria (4.42%), γ-proteobacteria (4.29%), Planctomycetacia (4.16%), Actinobacteria_c (4.00%), Betaproteobacteria (3.50%), EU686603_c (2.97%), Ktedonobacteria (2.91%), Acidimicrobiia (1.32%), Verrucomicrobiae (1.27%), Gemmatimonadetes_c (1.11%), Sphingobacteria (1.09%), and GU444092_c (1.06%). Bradyrhizobiaceae was the most dominant family with 22.83% followed by Acidobacteriaceae (10.62%), EU445199_f (5.72%), Planctomycetaceae (4.03%), Solibacteraceae (3.63%), FM209092_f (3.58%), Steroidobacter_f (2.81%), EU686603_f (2.73%), Hyphomicrobiaceae (2.33%), Ktedonobacteraceae (1.75%), AF498716_f (1.46%), Rhizomicrobium_f (1.03%), and Mycobacteriaceae (1.01%). Differences in the diversity of bacterial communities have more to do with geography than the impact on environmental factors and also the type of vegetation seems to affect the diversity of bacterial communities.

Perception and Preference of Elementary Schoolchildren on Rice Foods in Changwon and Gimhae City (창원.김해 지역 초등학생의 쌀음식에 대한 인식 및 기호도 조사)

  • Yun, Hyeon-Suk;Lee, Mi-Ja;Lee, Gyeong-Hye
    • Journal of the Korean Dietetic Association
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    • v.11 no.3
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    • pp.341-352
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    • 2005
  • This study was based on the information provided by 1,180 elementary school children (630 boys and 550 girls) of 5th or 6th grade in Changwon and Gimhae of Gyeongnam province. They were asked about perception and preference for rice foods by questionnaires. The purpose of this survey was to find the way of encouraging rice intake and preference which is currently getting lower because of increasing simple westernized eating habits, and to provide basic information needed for inheriting and improving our traditional rice-based dietary culture. The results are summarized as follows. Most of subjects (91.2%) thought that steamed rice is better than bread for own health. The reason why they chose to eat steamed rice was 'because it is good for health' (61.2%), and 'because it is staple food item that we eat everyday' (26.4%). Seventy one percent of girls and 52.7% of boys gave the answer 'because it is good for health' as the reason for eating steamed rice. The reasons for the importance of the rice-based dietary culture were 'because of its superior nutritional value' (40.8%), and 'because it is our traditional eating culture' (28.6%). While significantly more girls (42.9%) answered as 'because of its superior nutritional value', more boys (39.0%) answered as 'because it is our traditional dietary culture'. More boys (59.0%) preferred noodles than girls' (54.7%), and fruits were preferred more by girls than boys as substitution foods for steamed rice showing significant difference (p<0.01). They wanted rice product developed in the forms such as Ssalamyun (29.8%), Ssalmandoo (24.1%), rice noodles (20.6%), and rice bread (15.6%). The preference score on rice products of subjects was one dish meals (4.27) and drinks (4.26), snacks (3.72), convenience foods (3.61), and steamed rice (3.44) in order. Preferred food showing points over 4 were Ssalbap (plain steamed rice) (4.29) in steamed rice type food, Kimchi bokeumbap (4.56), Bokeumbap (4.55), Bibimbop (4.45), Omelet rice (4.44), Kimbap (4.42), Ddukkuk (4.33), Curried rice (4.33), Jajangbap (4.28), and Ddukmandookuk (4.24) in one dish meal type food, Samgak Kimbap (4.26) in convenience type food, Songpyun (4.48), Injulmi (4.18), Teokbokki (4.71), Ddukkochiguyi (4.46), and rice cookies (4.24) in snack type food, and Shikhye (4.61) and Misugaru (4.28) in drink type food. Based on these results, it may be said that elementary school children think the rice-based diet is good for health and this dietary culture should be inherited and developed not only in a traditional aspect but also in a nutritional aspect. Therefore, more studies are needed to develop various forms of rice food products and cooking recipes.

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The Significance of Gonadotropin Ratio in In Vitro Fertilization of Human Oocytes (성선 자극호르몬의 비율이 인간난자의 체외수정에 미치는 영향에 관한 연구)

  • Moon, Shin-Yong
    • Clinical and Experimental Reproductive Medicine
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    • v.15 no.2
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    • pp.135-147
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    • 1988
  • To compare the stimulation effect of the ratio in follicle stimulating hormone and luteinizing hormone in induction of multiple follicular growth, the serum $E_2$ level, the diameter of follicle, number of aspirated follicles and cleavage rate of in vitro fertilized preovulatory oocytes as well as the pregnancy rate were evaluated. Forty one patients with irreparable tubal disease were stimulated by hMG(n=24) or FSH/hMG(n=17) for the purpose of in vitro fertilization and embryo transfer. The following results were obtained. 1. Serum estradiol($E_2$) levels on the day of hCG administration were $921.0{\pm}353.3\;pg/ml$ in hMG group and $1272.9{\pm}1060.6\;pg/ml$ in FSH/hMG group. The serum $E_2$ value of hMG group was significantly lower than that of FSH/hMG group. 2. The diameter of leading follicle by ultrasonogram on the day of hCG administration were $16.2{\pm}2.0\;mm$ in hMG group and $16.2{\pm}2.6\;mm$ in FSH/hMG group. No significant difference of follicle diameter between two groups was demonstrated. 3. The number of follicles with diameter above 10 mm by sonogram on the day of hCG injection were $3.91{\pm}2.32$ in hMG group and $6.52{\pm}3.86$ in FSH/hMG group. There was significant difference of number of follicles between two groups, (p< 0.01). 4. The number of oocytes found per patient at aspiration were $2.59{\pm}1.00$ in hMG group and 3. $76{\pm}2.31$ in FSH/hMG group. There was significant difference of number of aspirated oocytes between two groups. (p< 0.05). 5. The detection rate of preovulatory oocyte at aspiration were 68.4%(39/57) in hMG group (n=22) and 77.6%(38/49) in FSH/hMG group (n=13). 6. The cleavage rate of preovulatory oocyte at 44 hours after insemination were 74.4%(29/39) in hMG group(n=22) and 81.6%(31/38) in FSH/hMG group (n=13). When only hMG was used, one pregnancy was established in 15 patients to whom 29 zygotes were transferred. And a full term normal female baby was delivered by elective cesarean section. In the FSH/hMG group, five pregnancies out of 9 transferred patients were confirmed by serum ${\beta}-hCG$. Two pregnancies were spontaneously aborted before the 6th week of pregnancy. One patient aborted her baby at the 18th week of pregnancy because of incompetent internal os of the cervix. Two patients delivered two full term babies by elective cesarean section. From the above findings, paralell with the increase in the ratio of exogenous follicle stimulating hormone to luteinizing hormone, an increase in oocyte recovery was observed as well as an improvements in pregnancy rate. It was concluded that FSH enrichment early in the follicular phase had a beneficial effect in the controlled ovarian hyperstimulation.

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Effects of Stroke Prevention Education Program of Oriental Nursing on Self-Health Perception Change, Health Behavior Change, Self-Care and Physiological Index of adult and elderly people (한방간호 중풍예방교육프로그램이 성인 및 노인의 자가 건강인식 변화, 건강 행태변화, 자가관리와 생리적 지수에 미치는 영향)

  • Chang, Koung-Oh;Oh, Mi-Jung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.1
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    • pp.111-120
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    • 2019
  • This study is a quasi experimental study to investigate the effects of stroke prevention education program of Oriental Nursing on self-health perception, health behavior change, self-care and physiological index of adults over 55 years old and elderly people. The data were collected from August 12 to November 29, 2017. The 53 study subjects comprised 26 experimental subjects who agreed to participate in the education program and 27 control subjects. In the results, there was no statistically significant difference between the experimental group and the control group in general characteristics and research variables before the program; further, the two groups were homogenous. In the hypothesis test, the change in self-health perceptions of the experimental and control groups was t = -5.28 (p <.001), health behavior change was t = -4.24 (p <.001), self-care was t = -5.28 (p <.05), systolic blood pressure was t = 2.48 (p <.05), diastolic blood pressure was t = 2.78 (p <.05), and cholesterol was t = -3.94 (p <.001). There were statistically significant differences between the two groups and all four hypotheses were supported, which demonstrated the effectiveness of the program. These study results suggest that it is an effective program to change the self-perception of health and health behavior, improve self-care ability, and control the physiological index. It is expected that these findings will be useful in developing a future effective oriental nursing intervention. The education program should be continuously carried out, and the area needs to be expanded.

Determinants of Homicide Locations Using Spatial Regression Analysis (공간회귀분석을 활용한 살인사건 영향요인 분석)

  • Lee, Soochang
    • Journal of the Korea Convergence Society
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    • v.10 no.5
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    • pp.203-211
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    • 2019
  • This study is to examine the impact of spatial characteristics of cities on homicide based on spatial econometric model. It selects housing types, racial heterogeneity, residential instability, overcrowding, commercial area, rate of 15 to 29 ages, and rate of the elderly as variables for spatial characteristics of cities. This study employs spatial regression analysis applying the spatial error model to analyze the data from 229 locals collected from Korean Statistical Information Service and Statistical Year Book of local governments. As a result, it shows that homicide has close relationships with apartment and multi-housing as housing types, racial heterogeneity, residential instability, and overcrowding, but not with the commercial area, rate of 15 to 29 ages, and rate of the elderly. The study contributes to expanding understanding and explanation on the causes of homicide focusing on social-structure approach for criminology by analyzing a more advanced model in applying variables than one of existing literature. This study suggests follow-up research on homicide based on both social-behavior approach and social-structure approach in the near future for the development of criminological theory.

Development and Application of Practical Ability Test for Pre-service Science Teacher (Female) (여성예비과학교사에 대한 교직수행능력검사도구의 개발과 적용)

  • Jang, Jyung-Eun;Kim, Sung-Won
    • Journal of The Korean Association For Science Education
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    • v.29 no.1
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    • pp.43-53
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    • 2009
  • The teacher's role in education is important. Science education majors must be able to solve problems effectively and pertinently when facing new ones in various situations and complicated human relations in order to become successful science teacher. The purpose of this research is to develop a test that measures the Practical Ability of pre-service science teachers and to apply this to them. The Practical Efficacy Scale for Science Education Majors was also developed in order to be used for validation. In this research, Practical Ability of Science Education Majors consisted of four sub-domains: subject education, business administration, relations and self-development. The result of the correlations between the scores of four sub-domains and the composite score of Practical Ability Test for Preservice Science Teacher(PATPST) is relevant. Subject education and administration business is the highest correlation with PATPSP score specially, and correlation between two areas appeared high. The result of applying PATPSP scores differed according to the grade of science education majors, but not according to their majors. This study's limitation is that the subjects consisted only of female students. However, PATPSP could be a new method that will help science education majors be successful science teachers.

Production of Dendropanax morbiferus extract containing multi-functional ingredients by serial fermentation using Bacillus subtilis HA and Lactobacillus plantarum KS2020 (고초균-젖산균의 순차적 복합 발효를 통한 복합 기능성 물질 함유 황칠나무 추출물의 생산)

  • Su-Jin Son;Hye-Mi Kang;Yun-Ho Park;Mi-Hyang Hwangbo;Sam-Pin Lee
    • Food Science and Preservation
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    • v.31 no.1
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    • pp.138-148
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    • 2024
  • The production of poly-γ-glutamic acid (γ-PGA) and γ-aminobutyric acid (GABA) was optimized by serial fermentation of Dendropanax morbiferus extract (DME) using Bacillus subtilis HA and Lactobacillus plantarum KS2020. The 1st alkaline fermentation was performed on 60% DME including 2% glucose and 10% monosodium ʟ-glutamate (MSG) as a precursor. The 1st fermented DME had 57 mg% tyrosine. Consequently, the 2nd lactic acid fermentation for 5 days increased the tyrosine content of 106 mg%. The mucilage containing γ-PGA showed a high content of 3.50% on the first day of alkaline fermentation and then increased to 4.10% after 2 days. The precursor (MSG) remaining in the 1st fermented DME was efficiently converted to GABA by the 2nd lactic acid fermentation in the presence of 5% skim milk, 1.5% glucose and 0.5% yeast extract, resulting in the production of 18.29 mg/mL GABA. The viable cells of lactic acid bacteria increased and indicated 9.49 log CFU/mL on the fermentation for 5 days, and the acidity of co-fermented DME indicated the highest value of 1.55%. Conclusively, the serial fermented DME has multi-functional ingredients containing γ-PGA, GABA, peptides and probiotics.

Changes in the Physicochemical and Antioxidant Characteristics during the Fermentation of Jujube Wine Using Hot Water Extract of Dried Jujube (건대추 열수추출물을 이용한 대추와인 발효중의 이화학 및 항산화적 특성 변화)

  • Eom, In-Ju;Choi, Jung-In;Kim, In-Ho;Kim, Tae-Hoon;Kim, Seong-Ho
    • Journal of Life Science
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    • v.26 no.11
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    • pp.1298-1307
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    • 2016
  • In the study, we investigated the optimum fermentation conditions as well as changes of physicochemical and antioxidant characteristics during the fermentation of jujube wine. The physicochemical characteristics of the jujube hot water extracts used in this study were a pH of 5.05, 0.01% acidity, and $6.5^{\circ}Brix$ concentration. For jujube wine fermentation, the optimal fermentation strain was selected among the isolated strains and the final chosen strain was identified as Saccharomyces cerevisiae, based on the 26S rRNA gene sequencing and similarity searching in GenBank DB. The jujube wine fermented with an initial $15^{\circ}Brix$ concentration of jujube extracts showed a maximum alcohol content of 13% and lower residual sugar concentration. Alcohol content during the jujube wine fermentation was increased after 3 days of fermentation, and no significantly difference after 6 days was found. The residual sugar concentration during the fermentation periods was significantly decreased with increasing alcohol content. The jujube wine properties at 12 days of fermentation were as follows: a pH of 4.34, acidity of 0.29%, alcohol content of 12.8%, and a residual sugar concentration of $8.70^{\circ}Brix$. The malic acid content in the organic acid of fermented jujube wine was significantly decreased during the fermentation proceeding, whereas the succinic acid and lactic acid contents were significantly increased. Antioxidant characteristics of the fermented jujube wine were appeared ABTS radical scavenging activity 45.80%, DPPH radical scavenging activity 61.89%, nitrite scavenging activity 91.95% and total polyphenol compound 3.69 mg/ml. In terms of consumer liking of the jujube wine by sensory evaluation, the color and overall acceptability of jujube wine were evaluated as more than average.