• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.2
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• pp.107-121
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• 2021

Skin hypopigmentation, which is observed in albinism or vitiligo, occurs when melanin synthesis is decreased by genetic, epigenetic, and other factors. To identify drug candidates that can promote melanin synthesis in cells, we screened an epigenetic modulator library consisting of 141 cell-permeable, small molecule drugs. B16/F10 murine melanoma cells were treated with each drug at 0.1 𝜇M and melanin synthesis and cell viability were subsequently monitored. As a result, (-)-neplanocin A, 3-deazaneplanocin A (DZNep), and DZNep hydrochloride were found to increase cellular melanin synthesis without causing cytotoxicity. Because these three structurally related drugs exhibited similar dose-dependent effects on melanin synthesis and cell viability, DZNep was selected as a representative drug for additional experiments. DZNep increased intracellular melanin content and tyrosinase (TYR) activity. DZNep also induced the expression of TYR, tyrosinase-related protein 1 (TYRP1), and dopachrome tautomerase (DCT) at the mRNA and protein levels. DZNep also induced the mRNA and protein expression of microphthalmia-associated transcription factor (MITF), a key regulator of melanin synthesis. DZNep is a specific inhibitor of S-adenosylhomocysteine hydrolase and it caused the accumulation of S-adenosylhomocysteine that inhibits histone methyltransferases in cells. This study suggests that melanogenesis can be modulated by targeting S-adenosylhomocysteine hydrolase in certain cellular contexts.

• The Journal of the Convergence on Culture Technology
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• v.7 no.3
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• pp.397-404
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• 2021

This study examined how different types of remote video lectures, which universities are using in earnest now due to the COVID-19 pandemic, impact the academic achievement of learners. Zoom-based remote video lectures were divided into two types-general lecture-centered and group task-oriented-and experimental and control groups were set up accordingly. An achievement test and a questionnaire were conducted to determine how academic achievement differed between these two approaches. The achievement test revealed no significant differences in achievement scores between the two groups. Meanwhile, the questionnaire regarding satisfaction with Zoom-based video lectures indicated that both groups were highly satisfied. Participants in the general lecture-centered type group cited the effect of learning, their instructors' class preparation and attitudes towards the class, and the promotion of participation in learning activities as the causes of their satisfaction, while participants in the task-oriented type group highlighted communication and sharing, the efficiency of group discussions, and the moderate difficulty level of group tasks as the reasons for their satisfaction with the video lectures. Remote video lectures in the untact era are becoming increasingly common, and educators will need to develop various Zoom-based teaching strategies to maximize their efficiency.

• Journal of the Korean Geosynthetics Society
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• v.21 no.1
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• pp.11-21
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• 2022

In this study, the individual vacuum seepage consolidation method, a new soft ground improvement method, was developed to supplement the conventional suction drain method (individual vacuum preloading method) and the geotechnical behavior was predicted through numerical analysis. If the individual vacuum seepage consolidation method applied, the effect of accelerating settlement and increasing the amount of settlement was high when the aquifer was located in the middle or at the bottom of the layer to the target improvement layer. It was found that the pumping amount in the aquifer does not affect the settlement behavior when it exceeds a certain level. Even vacuum pumping wells were installed in various locations, such as inside or outside of the embankment, the difference in settlement and horizontal displacement was insignificant. In addition, it was predicted that the settlement rate was the fastest and the horizontal displacement (inward) was large when both methods were carried out at the same time. Since this method can reach the target settlement amount very quickly, it was confirmed that it is possible to increase the spacing of vertical drain, thereby securing economic feasibility.

• Journal of Life Science
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• v.32 no.7
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• pp.501-509
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• 2022

Pollen corrected from Quercus species is abundant in Korea and has been used to treat anemia and inflammation of the prostate gland. It is also used for hemostasis in oriental medicine. In this study, the ethanol extract of the acorn pollen and its subsequent organic solvent fractions using hexane, ethylacetate, butanol, and water residue were prepared, and their antioxidant, anti-thrombosis, and hemolysis activities were evaluated. The ethylacetate fraction of acorn pollen (EF-AP) showed the highest polyphenol content (225.0 mg/g) and strong antioxidant activity among the solvent fractions. The RC₅₀ of EF-AP against DPPH, ABTS, and nitrite radicals was 72.2, 27.7, and 62.6 ㎍/ml, respectively. The blood coagulation activities of the all-solvent fractions determined by thrombin time, prothrombin time, and activated partial thromboplastin time were negligible up to 5 mg/ml. Platelet aggregation inhibitory activities were observed in the EF-AP, butanol fraction, and water residue, whereas hexane fraction induced strong platelet aggregation. The EF-AP has no hemolysis activity against human RBC up to 1 mg/ml. UPLC/MS/MS analysis of the EF-AP revealed that rutin, isoquercitrin, and astragalin are major compounds for antioxidant and anti-thrombosis activities. Our results suggest that EF-AP could be developed as a noble antioxidant and anti-thrombosis agent.

• Journal of the Korean Orthopaedic Association
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• v.55 no.3
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• pp.237-243
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• 2020

Purpose: This study examined the effects of the postoperative administration of parathyroid hormone (PTH) on fracture healing in intertrochanteric fractures accompanied by osteoporosis in elderly females. Materials and Methods: Female patients aged 65 years and more who underwent surgery after a diagnosis of intertrochanteric fractures and osteoporosis during the period from July 2013 to December 2017 were included as subjects. The subjects were divided into two groups: PTH-treated group and non-PTH-treated group. The formation time of the first callus, timing of the bridging callus, and time of bony union for both groups were evaluated. Results: In the PTH-treated group, the mean time of the first callus formation, average time of bridging callus, and the average time of bony union were 32, 58, 83 days, respectively, which were significantly shorter than that of the untreated group. Conclusion: PTH, a treatment for osteoporosis, promotes callus formation and the healing process. Therefore it will be helpful in intertrochanteric fractures accompanied by osteoporosis in elderly females.

• Journal of Life Science
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• v.32 no.8
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• pp.647-658
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• 2022

Particulate matter (PM) is known to be involved in the onset and progression of various diseases by promoting oxidative and inflammatory reactions as air pollutants containing various small particles that are harmful. In this study, the protective efficacy of herbal medicines was evaluated in human corneal epithelial cells (hCECs) to select natural products that can protect the eye, the primary organ directly exposed to external pollutants from PM. As a result, five candid ate herbal medicines [Cheonmundong, Asparagus Rhizome; Seokchangpo, Aciru Gramineri Rhizoma; Hwangryeon, Coptidis Rhizoma; Gamgug, Chrysanthemi Indici Flos; and Geumjanhwa (Marigold flower petals)] which showed inhibitory efficacy on PM_2.5-induced cytotoxicity, were selected from among 12 candidate herbal medicines. To evaluate the antioxidant activity of these candidate substances, the reactive oxygen species (ROS) scavenging ability was investigated, and it was found that the extracts of Seokchangpo, Cheonmundong and Hwangryeon showed a significant inhibitory effect on PM_2.5-induced ROS production, which was correlated with the preservation of mitochondrial activity. In addition, it was confirmed that they could block DNA damage caused by PM_2.5 through analysis of 8-hydroxy-2'-deoxyguanosine generation and phosphorylated-H2A histone family member X (γ- H2AX) expression. Furthermore, the increase in inflammasome activity and inflammatory response in PM_2.5-treated hCECs was also canceled in the presence of these extracts. Although additional studies are needed, the results of this study will be used as primary data to find novel natural compounds that protect hCECs from PM.

• Journal of Life Science
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• v.32 no.1
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• pp.44-50
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• 2022

The migration and proliferation of keratinocytes are key events in re-epithelization, itself a major phase in the wound healing process. Cuscuta japonica Choisy (CJC) is used as a traditional medicine to improve liver, heart, and intestinal function, and its extracts are reported to have various biological properties such as whitening, anti-oxidancy, and an anti-acne effect. However, it is not yet known in particular whether or not CJC essential oil (CJCEO) affects skin regeneration. In the present study, we isolated CJCEO by solvent extraction and tested its effect on wound healing responses using normal human keratinocytes, namely HaCaT cells. We found that CJCEO induced proliferation as well as migration in HaCaT cells in a dose-dependent manner. Compared with a control group, CJCEO treatment at 250 ㎍/ml increased proliferation by 239.98±5.51% in HaCaT cells in a dose and migration by 124.86±6.06%. Moreover, the oil induced sprout outgrowth and, at 250 ㎍/ml, increased collagen synthesis by 148.56±15.47% in HaCaT cells. These results demonstrate that CJCEO may promote skin regeneration and wound healing by increasing the migration, proliferation, and collagen synthesis of HaCaT cells. We therefore suggest that CJCEO could be used as a cosmetic material.

• The Journal of the Korea Contents Association
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• v.22 no.3
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• pp.309-321
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• 2022

As the spread of COVID19 has compelled activities in various fields to transform to adapt to the non-face-to-face environment, various activities have either already been transitioned into non-face-to-face methods or been searching for alternative methods to carry out activities in a non-face-to-face manner. However, there are apparent limits in handling this transition with the pre-existing digital technology. Ironically, said limitations are more apparent in the UX design field that has thus far emphasized resolutions based on digital technology. The reason for this stems from the nature of UX design which strongly emphasizes the importance of collaboration. Especially, in the field of UX design, problems are expected to surface under areas of communication and collaboration in workshops, which are productive means of collecting the ideas of interested parties and coming up with other new ideas. Based on the aforementioned rise of necessity, this study aims to assess the characteristics of workshops in the field of UX design and suggest an effective method of transitioning UX workshops into a non-face-to-face environment. Along the line of this process, this study has created a standard process in regards to design workshops with active creation, suggestion, and acceptance of ideas, among the various types of workshops defined by the Nielsen Norman Group. This study also developed a framework consisting of non-face-to-face workshops by combining with the standard process the methodologies of workshop activation and non-face-to-face services meant for communication and designing activities, and confirmed the adaptability and the effectiveness of said transition against various types of workshops. Application of the results of this study is expected to effectively lead the transition into the non-face-to-face environment and improve the collaborative efforts of the interested parties via workshops.

• Korean small business review
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• v.42 no.3
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• pp.275-304
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• 2020

The purpose of this study is to present implications for revitalizing start-ups and contribute to enhancing the success rate of start-ups by clarifying factors and processes for converting workers with knowledge, experience and networks in related fields into entrepreneur. Based on the Shapero's Entrepreneurial Event Model, this study demonstrated whether the job insecurity and entrepreneurship of the workers were precipitating events of the entrepreneurial intention and whether the perceived desirability and feasibility of the entrepreneurial behaviour mediated between them. According to the results of the study, first, it was confirmed that job insecurity, innovativeness, and risk-taking of workers are factors that increase the entrepreneurial intention. Second, the indirect effect of perceived desirability between all components of job insecurity and entrepreneurial intentions was not significant, but all components of entrepreneurship appeared to improve entrepreneurial intention through perceived desirability. Third, it has been confirmed that job insecurity, innovativeness, and risk-taking strengthen the entrepreneurial intention through the perception of feasibility for entrepreneurial behavior. Through this study, it is confirmed that in order to convert workers into entrepreneur, it is necessary to strengthen entrepreneurship education and support for internal ventures for workers to increase their positive attitude and confidence in implementation. Therefore, it is expected to help solve job problems and revive the sluggish economy by contributing to boosting start-ups.

• Korean small business review
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• v.42 no.4
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• pp.251-283
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• 2020

The purpose of this study is to provide assistance to the establishment of related policies to improve the level of acceptance and use of smart factories for SMEs in Korea. To this end, the Unified Technology Acceptance Model (UTAUT) was extended to select additional factors that could affect the intention to accept technology, and to demonstrate this. To achieve the research objective, a questionnaire composed of 7-point Likert scales was prepared, and a survey was conducted for manufacturing-related companies. A total of 136 questionnaires were used for statistical processing. As a result of the hypothesis test, performance expectation and social influence had a positive (+) positive effect on voluntary use, but effort expectation and promotion conditions did not have a significant effect. As an extension factor, the network effect and organizational characteristics had a positive (+) effect, and the innovation resistance had a negative effect (-), but the perceived risk had no significant effect. When the size of the company is large, the perceived risk and innovation resistance are low, and the level of influencing factors for veterinary intentions, veterinary intentions, and veterinary behaviors are excluded. Through this study, factors that could have a positive and negative effect on the adoption (reduction) of smart factory-related technologies were identified and factors to be improved and factors to be reduced were suggested. As a result, this study suggests that smart factory-related technologies should be accepted.