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http://dx.doi.org/10.5352/JLS.2022.32.1.44

Effect of Essential Oil from Cuscuta japonica CHOISY on Proliferation and Migration in Human Skin Keratinocyte  

Choi, In Ho (Department of Plant and Biomaterials Science, Gyeongsang National University)
Kim, Do Yoon (Division of Cosmetics and Biotechnology, College of Life and Health Science, Hoseo University)
Lee, Hwan Myung (Division of Cosmetics and Biotechnology, College of Life and Health Science, Hoseo University)
Publication Information
Journal of Life Science / v.32, no.1, 2022 , pp. 44-50 More about this Journal
Abstract
The migration and proliferation of keratinocytes are key events in re-epithelization, itself a major phase in the wound healing process. Cuscuta japonica Choisy (CJC) is used as a traditional medicine to improve liver, heart, and intestinal function, and its extracts are reported to have various biological properties such as whitening, anti-oxidancy, and an anti-acne effect. However, it is not yet known in particular whether or not CJC essential oil (CJCEO) affects skin regeneration. In the present study, we isolated CJCEO by solvent extraction and tested its effect on wound healing responses using normal human keratinocytes, namely HaCaT cells. We found that CJCEO induced proliferation as well as migration in HaCaT cells in a dose-dependent manner. Compared with a control group, CJCEO treatment at 250 ㎍/ml increased proliferation by 239.98±5.51% in HaCaT cells in a dose and migration by 124.86±6.06%. Moreover, the oil induced sprout outgrowth and, at 250 ㎍/ml, increased collagen synthesis by 148.56±15.47% in HaCaT cells. These results demonstrate that CJCEO may promote skin regeneration and wound healing by increasing the migration, proliferation, and collagen synthesis of HaCaT cells. We therefore suggest that CJCEO could be used as a cosmetic material.
Keywords
Collagen synthesis; Cuscuta japonica CHOISY; keratinocyte; migration; proliferation;
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1 Suk, K. D.,Lee, S. H. and Shin, K. K. 2004. Growth-inhibitory effects of Cuscuta japonica Choisy's and C. australis R.Be's extracts against Propionibacterium acnes. Kor. J. Pharmacogn. 35, 375-379.
2 Chang, S. J. and Suk, K. D. 2006. Inhibitory effects on melanin biosynthesis and tyrosinase, cytotoxicity in clone M-3 and antioxidant activity by Cuscuta japonica, C. australis, and C. chinensis extracts. Yakhak Hoeji 50, 421-428.
3 Garcia, J. L., Asadinezhad, A., Pachernik, J., Lehocky, M., Junkar, I., Humpolicek, P., Saha, P. and Valasek, P. 2010. Cell proliferation of HaCaT keratinocytes on collagen films modified by argon plasma treatment. Molecules 15, 2845-2856.   DOI
4 Haase, I., Evans, R., Pofahl, R. and Watt, F. M. 2003. Regulation of keratinocyte shape, migration and wound epithelialization by IGF-1-and EGF-dependent signalling pathways. J. Cell Sci. 116, 3227-3238.   DOI
5 Santoro, M. M. and Gaudino, G. 2005. Cellular and molecular facets of keratinocyte reepithelization during wound healing. Exp. Cell Res. 304, 274-286.   DOI
6 Jin, M. H., Park, S. K. and Hwang, S. J. 2018. Composition for enhancing elasticity and whitening. Korea patent 10-1909237.
7 Joe, W. A., Cheon, S. J., Jang, M. J., Sung, J. Y., Jung, S. H., Kang, B. Y., Choi, E. Y., Jeong, Y. S., Lee, J. S., Choi, H. J., Baek, W. J., An, B. J., Lee, C. E. and Lee, J. T. 2006. Study of the cosmeceutical activities of Cuscuta japonica Choisy. Kor. J. Herbology 21, 103-108.
8 Lee, M. Y. and Joo, J. S. 1989. Effect of Cuscutae extract on experimentally induced liver damage in rabbits. J. Kor. Soc. Food Nutr. 18, 333-337.
9 O'Toole, E. A. 2001. Extracellular matrix and keratinocyte migration, Clin. Exp. Dermatol. 26, 525-530.   DOI
10 Perlish, J. S., Lemlich, G. and Fleischmajer, R. 1988. Identification of collagen fibrils in scleroderma skin. J. Invest. Dermatol. 90, 48-54.   DOI
11 Petersen, M. J., Woodley, D. T., Stricklin, G. P. and O'Keefe, E. J. 1990. Enhanced synthesis of collagenase by human keratinocytes cultured on type I or type IV collagen. J. Invest. Dermatol. 94, 341-346.   DOI
12 Schultz, G. S. and Wysocki, A. 2009. Interactions between extracellular matrix and growth factors in wound healing. Wound Repair Regen. 17, 153-162.   DOI
13 Suk, K. D., Lee, S. H. and Bae, J. M. 2004. Inhibitory effects of Cuscuta japoinca extract and C. australis extracts on mushroom tyrosinase activity. Kor. J. Pharmacogn. 35, 380-383.
14 Yang, B., Ji, C., Kang, J., Chen, W., Bi, Z. and Wan, Y. 2009. Trans-Zeatin inhibits UVB-induced matrix metalloproteinase1 expression via MAP kinase signaling in human skin fibroblasts. Int. J. Mol. Med. 23, 555-560.
15 Sundarraj, S., Thangam, R., Sreevani, V., Kaveri, K., Gunasekaran, P., Achiraman, S. and Kannan, S. 2012. γ-Sitosterol from Acacia nilotica L. induces G2/M cell cycle arrest and apoptosis through c-Myc suppression in MCF-7 and A549 cells. J. Ethnopharmacol. 141, 803-809.   DOI
16 Tuan, T. L., Keller, L. C., Sun, D., Nimni, M. E. and Cheung, D. 1994. Dermal fibroblasts activate keratinocyte outgrowth on collagen gels. J. Cell Sci. 107, 2285-2289.   DOI
17 Xue, M., Thompson, P., Kelso, I. and Jackson, C. 2004. Activated protein C stimulates proliferation, migration and wound closure, inhibits apoptosis and upregulates MMP-2 activity in cultured human keratinocytes. Exp. Cell Res. 299, 119-127.   DOI
18 Yoon, M. S., Won, K. J., Kim, D. Y., Hwang, D. I., Yoon, S. W., Kim, B. and Lee, H. M. 2014. Skin regeneration effect and chemical composition of essential oil from Artemisia Montana. Nat. Prod. Commun. 9, 1619-1622.
19 Hong, S. P. 2010, Bricks and mortar: two compartment system. Kor. Soc. Skin Bar. Res. 12, 35-40.
20 Gibbs, S., Backendorf, C. and Ponec, M. 1996. Regulation of keratinocyte proliferation and differentiation by all-trans-retinoic acid, 9-cis-retinoic acid and 1, 25-dihydroxy vitamin D3. Arch. Dermatol. Res. 288, 729-738.   DOI
21 Balamurugan, R., Duraipandityan, V. and Ignacimuthu, S. 2011. Antidiabetic activity of γ-Sitosterol isolated from Lippia nodiflora L. in strptozotocin induced diabetic rats. Eur. J. Pharmacol. 667, 410-418.   DOI
22 Heon, Y. H., Kim, M. H. and Lee, M. R. 2008. Antioxidative and antimutagenic activity of ethanol extracts from Cuscutae Semen. Kor. J. Food Cook. Sci. 24, 46-51.
23 Huang, C., Ma, W. Y., Dawson, M. I., Rincon, M., Flavell, R. A. and Dong, Z. 1997. Blocking activator protein-1 activity, but not activating retinoic acid response element, is required for the antitumor promotion effect of retinoic acid. Proc. Natl. Acad. Sci. USA. 94, 5826-5830.   DOI
24 Oh, D. H., Ham, S. S., Park, B. K., Ahn, C. and Yu, J. Y. 1998. Antimicrobial activities of natural medicinal herbs on the food spoilage or foodborne disease Microorganisms. Kor. J. Food Sci. Technol. 30, 957-963.
25 Kim, D. Y., Hwang, D. I., Yoon, M. S., Choi, I. H. and Lee, H. M. 2016. Effect of hydrosol extracted from Chrysanthemum boreale Makino flower on proliferation and migration in human skin keratinocyte. J. Soc. Cosmet. Sci. Kor. 42, 95-101.   DOI
26 Kim, M. S., Park, T. J., Lim, J. S. and Kim, S. Y. 2019. Effect of Agaricus biorenovate extract on collagen synthesis and matrix metalloproteinase-1 production in human dermal fibroblast. KSBB J. 34, 49-53.   DOI
27 Li, C. M., Liu, Y., Xie, Z. and Lu, Q. L. 2015. Stigmasterol protects against Ang II-induced proliferation of A7r5 aortic smooth muscle cell-line. Food Funct. 6, 2266-2272.   DOI