• Journal of the Korea Organic Resources Recycling Association
• /
• v.19 no.2
• /
• pp.41-48
• /
• 2011

In this study, the soil remediation by landfarming was carried out using microbial activators. Feasibility studies and reduction capacity of TPH(Total Petroleum Hydrocarbons) were investigated in order to find out how fast and eco-friendly the contaminated soil can be recovered. The lab-test confirmed not only the performance and degradation efficiency of microbial activators but also the effect of TPH reduction in the contaminated soil. The optimum growth conditions for indigenous microorganisms were identified using microbial activators. Based on the results of TPH removal, although there had been a little of difference in between natural decomposition and microbial activators until 20 days, the sample groups of microbial activators were higher than the control ones after 20 days. Microbial activators were applied to the field experiments on landfarming. Based on the results of removal rate in each floor of soil, it was found that the removal rates were 85.8 % in the upper, 84.4 % in the middle, and 66.10 % in the bottom. Considering that the reduction rate of TPH for the control group averaged 71.1%, the microbial activators might not be fully transferred into the bottom, which resulted from the piles of soil. As the piles have already reached 1 m in the field experiments, the low piles of soil under 0.6 m may enhance the treatment efficiency of TPH.

• Korean Journal of Food Science and Technology
• /
• v.36 no.4
• /
• pp.683-687
• /
• 2004

Raw beefs used fer Jangzorim production were evaluated fur contamination of pathogenic bacteria and microorganisms related to spoilage and food safety. Eleven groups of mesophilic, psychrotrophic, and anaerobic microorganisms, and total coliforms were selected to evaluate degree of food contamination. Nine strains including Bacillus cereus, Clostridium botulinum, C. perfringens, and Listeria monocytogenes were selected to evaluate incidences of pathogenic bacteria. Raw beefs harbored large populations of microorganisms, which decreased greatly after heat treatment. Psychrotrophic microorganisms were found to be more abundant than other microorganisms. B. cereus, C. perfringens, and L. monocytogenes were isolated from raw beefs, whereas C. botulinum, Escherichia coli O157:H7, Salmonella spp., Shigella spp., Staphylococcus aureus, and Yersinia enterocolitica were not isolated. Isoiates from Cereus selective agar, clostridium Perfringens agar, and Oxford agar were in 99.8, 99.9 and 98.6% agreements with B. cereus, C. perfringens, and L. monocytogenes at species level, respectively. B. cereus produced enterotoxin with CRET-RPLA method, whereas C. perfringens did not produce enterotoxin with PET-RPLA method.

• Korean Journal of Soil Science and Fertilizer
• /
• v.36 no.4
• /
• pp.239-246
• /
• 2003

We examined the relationships among community composition, microbial population, and microbial biomass to determine whether different land use leads to differences in microbial community composition. And also the relationships between soil characteristics and microbial community composition were investigated. There was no difference in pH between uncultivated and cultivated soils, but electrical conductivity, and contents of organic matter, available P and exchangeable cations were greater in the cultivated soil compared to the uncultivated soil. A linear correlation ($r^2=0.557$, n=18, p<0.01) was found between biomass-C estimated with fumigation extraction technique and total amount of fatty acids. An increase of fatty acid methyl esters (FAMEs) for bacteria, actinomycetes, fungi and protozoa was observed in cultivated soil.

• Korean Journal of Soil Science and Fertilizer
• /
• v.43 no.2
• /
• pp.194-199
• /
• 2010

Compost as a soil amendment is of importance in enhancing the soil chemical and microbial qualities; however, soil microbial community can vary depending on the composition, and the amount of compost applied to plant in the soil. Responses of soil microbial properties to compost applications with 0, 30, and 60 Mg $ha^{-1}$ were investigated in silt loam soils where red pepper(Capsicum annuum L.) was mainly cultivated in Yeongyang, Gyeongbuk, Korea. The analysis of phospholipid fatty acids (PLFAs) extracted from soil showed that compost amounts significantly increased PLFAs representing as bacteria, fungi, and VAM-fungi as well as the ratio of fungi/bacteria, and monounsaturated/saturated PLFAs. Increasing the amount of compost significantly increased Gram-/Gram+ PLFAs' ratio, but significantly decreased monounsaturated/saturated PLFAs' ratio. Therefore, this result shows that compost would vary to a limited extent the microbial community in red pepper field. However, increase in compost application would change the subgroup structure of microbial community only.

• Microbiology and Biotechnology Letters
• /
• v.33 no.1
• /
• pp.56-64
• /
• 2005

The occurrence and abundance of protozoa at advanced wastewater treatment plant were compared with operating parameters and effluent quality using statistical procedures. It seemed that plant operating conditions influenced the distribution of protozoa in the mixed liquor. In statistical analysis, the distribution of protozoa showed the operating condition of plant and predicted effluent quality. Once enough data concerning protozoa, operating parameters and effluent has been gathered, the operator has a valuable tool for predicting plant performance and near-future effluent quality based on microscopic examination. Plant operator manipulates operating conditions if he knows near-future effluent quality is deteriorating. Perhaps more importantly it can be used to actually control the plant to adjust the operating conditions to obtain the protozoal populations that have been shown to provide the best effluent quality.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.10 no.4
• /
• pp.86-95
• /
• 2002

Anaerobic digestion of municipal solid waste(MSW) is recently getting attention due to energy generation and abatement of global warming. MSW has high solid content and low nitrogen content. Its major component is cellulose and hemicellulose. The conversion rate of organic portion of MSW to methane is approximately 50%, representing $0.2m^3/kg$ VS. Long hydraulic retention time is required for high solid content and inoculum should be mixed with the feed. When MSW is digested anaerobically, maximum limit of C/N ratio is 25 and the optimum concentration of $NH_3-N$ is 700mg/L. lime and sodium bicarbonate are used to adjust pH. Excess addition of sodium bicarbonate above 3,500mg/L will cause sodium toxicity. Thermophilic anaerobic digestion is effective in the control of pathogen although its operation and maintenance is difficult. To optimize the anaerobic digestion of MSW, it is necessary to understand the mechanism of microorganims involved in anaerobic digestion.

• Korean Journal of Soil Science and Fertilizer
• /
• v.45 no.5
• /
• pp.792-797
• /
• 2012

Glomalin-related soil protein has been suggested as an enhancer for soil stability by promoting the aggregation. In this study, we examined the concentrations of glomalin and characteristics of microbial community in 20 paddy soils sampled from Gyeongnam Province. Total soil glomalin as glomalin-related soil protein (GRSP) had a significant positive correlation with soil organic matter (p<0.01) and soil dehydrogenase activity (p<0.01). The concentration of GRSP significantly correlated to soil microbial biomass carbon (p<0.001) and the total bacterial community (p<0.01) in paddy soils. In addition, the GRSP had a significant positive correlation with gram-negative bacteria community (p<0.05) and ratio of cy19:0 to 18:$1{\omega}7c$ (p<0.05) in paddy soils. In conclusion, the concentration of GRSP could be an indicator of soil health that simplify the inspection steps for sustainable agriculture in paddy soils.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.14 no.5
• /
• pp.545-550
• /
• 2004

The study of available genotypes (biodiversity analysis) in bacterial communities is of growing importance in several fields such as ecology, environmental technology, clinical diagnostics, etc. These culture-independent genotyping techniques, especially amplifying 16S rRNA genes, attempt to overcome some shortcomings of conventional cultivation method. Biodiversity analysis based on molecular technique were laborious for base-calling chromatogram, trimming primer sites, correcting strand directions, electing representative operation taxonomic units (OTU), etc. Also, biologists wanted intuitively to confirm results of the above processes. For making up these demands, we developed the web application based on Folder-Process-Filter (FPF) modeling with correspondence to classical Model-View-Controller model. The model of web application leads to keep virtues of simplicity and directness for development and management of the stepwise web interfaces. The web application was developed in Perl and CGI on Linux workstation. It can be freely accessed from http://home.pusan.ac.kr/～genome/tools/rat.htm.

• Korean Journal of Soil Science and Fertilizer
• /
• v.44 no.5
• /
• pp.819-823
• /
• 2011

The present study evaluated the seasonal changes of the soil microbial population by selected media in an organic farming system (OFS) with rye rotation cropping management compared to those in a conventional farming system (CFS) with chemical fertilizers, pesticide and herbicide from May 2009 to October 2010 in an upland field. With the exception of fungi, populations of aerobic bacteria, Gram-negative bacteria, and Bacillus spp. were higher in the OFS soil during soybean-growing stages. In addition, populations of aerobic bacteria, Gram-negative bacteria, and Bacillus spp. in the OFS soil were nearly two times more than those in the CFS soil on reproductive growth stages. Our findings suggested that Bacillus spp. should be considered as responsible factor for microbial population differentiation observed between the OFS and the CFS in upland fields.

• Journal of Applied Biological Chemistry
• /
• v.55 no.1
• /
• pp.47-53
• /
• 2012

Increasing concerns over green farming technology, plant growth promoting rhizobacterium (PGRP) having growth promoting as well as plant disease suppressing properties was recently preferred to use for biological control of plant pathogens infecting plant. We measured the influence of the selected microbial consortium agents-a mixture of PGPR strains-, commercial bio-fungicide, and chemical pesticides on soil microbial community in red pepper field. The activities of soil enzyme such as dehydrogenase, urease, phosphatase, ${\beta}$-glucosidase, and cellulase were analyzed to investigate that of soil microbial community. We also measured plant length, main stem, stem diameter, number of branches and yields of red-pepper in order to observe the red pepper growth promotion. The results of measuring enzyme activities were dehydrogenase 3.5584 ${\mu}g$ TPF $g^{-1}h^{-1}$, urease 15.8689 ${\mu}g$ $NH_4{^-}N$ $g^{-1}h^{-1}$, phosphatase 0.5692 ${\mu}g$ PNP $g^{-1}h^{-1}$, ${\beta}$-glucosidase 2.4785 ${\mu}g$ PNP $g^{-1}h^{-1}$, and cellulase 86.1597 ${\mu}g$ glucose $g^{-1}h^{-1}$ in the soil treated with the microbial consortium agents, so it came out to be very active in the soil. Observing the growth of red-peppers, the main-stem length and the stem diameter were 6.1% and 8.1% higher in the soil treated with the selected microbial consortium agent than the chemical pesticides. After harvesting, yields were 7.3% higher in the soil treated with selected microbial consortium agents than the chemical pesticides. These results showed that microbial consortium agents contribute to increasing soil microbial diversity, growth promoting, and yield of red pepper.