Browse > Article

Evaluation of Microbial Community Composition in Cultivated and Uncultivated Upland Soils by Fatty Acids  

Suh, Jang-Sun (National Institute of Agricultural Science and Technology)
Chon, Gil-Hyong (National Institute of Agricultural Science and Technology)
Kwon, Jang-Sik (National Institute of Agricultural Science and Technology)
Kim, Sang-Hyo (National Institute of Agricultural Science and Technology)
Baek, Hyung-Jin (National Institute of Agricultural Biotechnology)
Publication Information
Korean Journal of Soil Science and Fertilizer / v.36, no.4, 2003 , pp. 239-246 More about this Journal
Abstract
We examined the relationships among community composition, microbial population, and microbial biomass to determine whether different land use leads to differences in microbial community composition. And also the relationships between soil characteristics and microbial community composition were investigated. There was no difference in pH between uncultivated and cultivated soils, but electrical conductivity, and contents of organic matter, available P and exchangeable cations were greater in the cultivated soil compared to the uncultivated soil. A linear correlation ($r^2=0.557$, n=18, p<0.01) was found between biomass-C estimated with fumigation extraction technique and total amount of fatty acids. An increase of fatty acid methyl esters (FAMEs) for bacteria, actinomycetes, fungi and protozoa was observed in cultivated soil.
Keywords
Fatty acid; Fatty acid methyl ester; Microbial community; Upland soil;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Frostegard, A., and E. Baath. 1996. The use of phospholipid fatty acid to estimate bacterial and fungal biomass in soil. Biol. Fertil, Soils 22:59-65   DOI
2 Griffiths, B.S., K. Ritz, N. Ebblewhite, and G. Dobson. 1999. Soil microbial community structure: effects of substrate loading rate. Soil Biol. Biochem. 31:145-153   DOI   ScienceOn
3 Kowalchuk, G.A., J.R. Stephen, J.I. De Boer, J.I. Prosser, M.T. Embley, and J.W. Woldendorp. 1997. Analysis of proteobacteria ammonia-oxidizing bacteria in coastal dunes using denaturing gradient gel electrophoresis and sequencing of PCR amplified 16S rDNA fragments. Appl. Environ. Microb. 63:1489-1497
4 Pankhurst, C.E., S. Yu, B.G. Hawke, and B.D. Harch. 2001 Capacity of fatty acid profiles and substrate utilization patterns to describe differences in soil microbial communities associated with increased salinity or alkalinity at three locations in South Australia. Biol. Fertil. Soils 33:204-217   DOI   ScienceOn
5 Zelles, L. 1999. Fatty acid patterns of phospholipids and 1ipopo1i/saccharides in the characterization of microbial communities in soil: a review. Biol. Ferdl. Soils 29:111-129   DOI   ScienceOn
6 Zelles, L. 1997. Phospholipid fatty acid profiles in selected members of soil microbial communities. Chemosphere 35:275-294   DOI   PUBMED   ScienceOn
7 Baath, E., and T.H. Anderson. 2003. Comparison of soil fungal/bacterial ratios in a pH gradient using physiological and PLFA-based techniques. Soil Biol. Biochem. 35:955-963   DOI   ScienceOn
8 Muyzer, G., E.C. De Waal, and A.G. Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microb. 59:695-700
9 Ravnskov, S., 0. Nybroe, and I. Jakobsen. 1999. Influence of an arbuscular mycorrhizal fungus on Pseudomonas fluorescens DF57 in rhizosphere and hyphosphere soil. NewPhytol. 142:113-122
10 White, D., J. Stair, and D. Ringelberg. 1996. Quantitative comparisons of in situ soil microbial biodiversity by signature biomarker analysis. J. Ind. Mirobiol. Biot. 17:185-196   DOI   ScienceOn
11 Fang, C., M. Radosevich, and J.J. Fuhrmann. 2001 Characterization of rhizosphere microbial community structure in five similar grass species using FAME and B10L0G analyses. Soil Biol. Biochem. 33:679-682   DOI   ScienceOn
12 Suh, J.S., J.S. Kwon, and S.H. Kim. 2002. Microbial diversity, survival and recovery as bioindicators in soils from different parent materials in Korea. Korean J. Soil Sci. Fert. 35:243-252
13 Ratledge, C., and S.G. Wilkinson. 1988. Microbial lipids. Vol. 1. Academic Press, London, UK
14 Waldrop, M.P., T.C. Balser, and M.K. Firestone. 2000. Linking microbial community composition to function in a tropical soil. Soil. Biol. Biochem, 32:1837-1846   DOI   ScienceOn
15 Bossio, D.A., and K.M. Scow. 1998. Impacts of carbon and flooding on soil microbial communities; phospholipid fatty acid profiles and substrate utilization patterns. Microb. Ecol. 35:265-278   DOI   ScienceOn
16 Dierksen, K.P., G.W. Whittaker. G.M. Banowetz, M.D. Azevedo, A.C. Kennedy, J.J. Steiner. and S.M. Griffith. 2002. High resolution characterization of soil biological communities by nucleic acid and fatty acid analyses. Soil Biol. Biochem. 34:1853-1860   DOI   ScienceOn
17 Kourtev, P.S., J.G. Ehrenfeld, and M. Haggblom. 2003. Experimental analysis of the effect of exotic and native plant species on the structure and function of soil microbial communities. Soil Biol. Biochem. 35:895-905   DOI   ScienceOn
18 Ihekwe, A.M., and A.C. Kennedy. 1999. Fatty acid methyl ester (FAME) Profiles as a tool to investigate community structure of two agricultural soils. Plant Soil 206:151-161
19 Ritchie, N.J., M.E. Schutter, R.P. Dick, and D.D. Myrold. 2000. Use of length heterogeneity PCR and fatty acid methyl ester profiles to characterize microbial communities in soil. Appl. Environ. Microbiol. 66:1688-1675
20 Suh, J.S., and J.S. Shin. 1997. Soil microbial diversity of paddy fields in Korea. Korean J. Soil Sci. Fert. 30:200-207