• Title/Summary/Keyword: 종균열

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Comparison of productivity among various spawn shapes of middle-temperature type strain for sawdust cultivation of Shiitake (표고 톱밥재배시 중온성 품종의 종균 형태에 따른 생산성 비교)

  • Lee, Bong-Hun;Bak, Won-Chull;Ka, Kang-Hyeon;Ryu, Sung-Ryul
    • Journal of Mushroom
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    • v.6 no.1
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    • pp.38-41
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    • 2008
  • Studies were processed to confirm the difference of the shiitake productivity according to different spawn shapes(sawdust, plug-shaped and liquid spawns) on middle-temperature type strain. A tendency of fruiting was similar among three treatments, and treatments inoculated with plug-shaped spawn and liquid spawn produced over 50 % of total yield until 2nd flushing period and 80 % of total yield until 4th flushing period. In investigation of deformed fruit-bodies, all of three treatments occurred until 2nd flushing period. However, in rate of deformed fruit-bodies, treatment inoculated with liquid spawn was lower than others. In investigation of yield, the amount produced on treatment inoculated with liquid spawn was 411 g per medium and it was highest among treatments. And the amount of fruit-bodies over 10 g was higher than others. Also, the number of fruit-bodies between sawdust and plug-shaped spawn was different, but each weight of fruit-bodies among three treatments was not different.

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Comparison of Productivity according to Different Quantity of Sawdust and Liquid Spawn for Sawdust Cultivation of Shiitake (표고톱밥재배시 톱밥종균과 액체종균의 접종량 차이에 따른 생산성 비교)

  • Bak, Won-Chull;Lee, Bong-Hun;Ka, Kang-Hyeon;Ryu, Sung-Ryul
    • The Korean Journal of Mycology
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    • v.36 no.1
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    • pp.41-44
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    • 2008
  • Studies were made to investigate the difference of the shiitake productivity according to the use of sawdust and liquid spawn on middle-temperature type strain. Treatments inoculated with sawdust spawn have shown that yields were dispersed according to flushing periods and fruit-bodies occurred sporadically on medium. However, treatments inoculated with liquid spawn have shown that yield was relatively concentrated at 1st flushing period and fruit-bodies were densely occurred on medium. At total yield and the number of fresh fruit-bodies until seventh flushing, treatments inoculated with liquid spawn were more than those inoculated with sawdust spawn. Also, biological efficiency (B.E.) of inoculation with 20 ml liquid spawn which produced 30% of the medium weight was highest as 69%, but biological efficiency of inoculation with 10 g sawdust spawn was lowest as 42%. And, although rate of fruit-bodies over 10 g in total yield was high on treatments inoculated with sawdust spawn, amounts of fruit-bodies over 10 g were not different among treatments.

Culture Condition and Inoculum Volume of Liquid Spawn on the Bottled Cultivation of Agrocybe cylindracea (버들송이(Agrocybe cylindracea)의 액체종균 배양조건과 접종량)

  • Cheong, Jong-Chun;Hong, In-Pyo;Jang, Kab-Yeul;Park, Jeong-Sik
    • The Korean Journal of Mycology
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    • v.31 no.2
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    • pp.94-97
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    • 2003
  • In order to shorten cultivation periods of Agrocybe cylindracea, liquid spawn was used in place of sawdust spawn. Optimum culture condition of liquid spawn was PSB (potato extract sugar broth) medium, $25^{\circ}C$ temperature and pH 7.0. And, optimum inoculum volume was $10{\sim}15ml$. Two grams per liter of dry weight of the fungal mycelia were obtained with 10l of culture bottle with liquid spawn after 10 days. In 850 ml-bottle of sawdust media, cultivation period using liquid spawn (30 days) was five days shorter compared with the cultivation period in sawdust spawn (35 days). Also, complete growth of sawdust media was increased to 92% (liquid spawn) from 75% (sawdust spawn) and yield of fruiting bodies was increased to about 10%.

Analysis and cause of occurrence of lining cracks on NATM tunnel based on the precise inspection for safety and diagnosis - Part I (정밀안전진단 결과를 활용한 NATM터널(무근)의 라이닝 균열 종류별 발생원인 및 분석 - Part I)

  • Choo, Jin-Ho;Park, Sung-Woo;Kim, Hyung-Tak;Jee, Kee-Hwan;Yoon, Tae-Gook
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.13 no.3
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    • pp.199-214
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    • 2011
  • A crack is the main cause to affect the integrity of tunnel lining as well as leakage, spalling, exposed rebar, corrosion, carbonation and so on. Since the 1980, NATM has prevailed on excavation method and geotechnical philosophy in tunnel. Although the pattern of cracks has been reported by several engineers' effort, it was only focused on longitudinal cracks of lining. Eleven operational NATM tunnels have been conducted with the precise inspection for safety and diagnosis by KISTEC (Korea Infrastructure Safety and Technology Incorporation). With those results, the crack patterns by the spatial distribution and appearance for each tunnel have been analyzed and the cause of occurrence for seven common types of cracks in NATM tunnels was classified. Additionally, the longitudinal crack on lining above duct slab was figured out by numerical simulation and field inspection. Each crack has been analyzed by CCD (Charge-Coupled Device) scanner image with 3D configuration. Each type of cracks is also explained with output of experimental and condition of construction. Defined cracks on NATM tunnels will be good example for periodical inspection and precise inspection for safety and diagnosis.

Development of a spawning method using liquid inoculum of Agaricus bisporus (양송이 액체접종원을 이용한 종균 제조 방법 개발)

  • Oh, Youn-Lee;Jang, Kab-Yeul;Oh, Min Ji;Im, Ji-Hoon
    • Journal of Mushroom
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    • v.19 no.2
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    • pp.109-113
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    • 2021
  • Currently, the spawn of the mushroom Agarcus bisporus is produced by a method developed in the 1980s, and anew spawning method needs to be developed to improve the quality of the spawn. In this study, the condition for a maximum mycelium weight(5.92±0.52 g/L) was shaking culture (24 hours/day) at 24℃ and 120 rpm in CDB (compost dextrose broth). Based on this, the ventilated liquid culture method (2.5 L/min) was cultured for 10 days. This method was appropriate, andwhen the inoculum was cultured at 50 g/mL for about 10 days, it was cultured well without agglomeration and shaking of seed.

Small-Dimensional Thermo-optic MMI Switch with Improved Response-time (MMI를 이용한 빠른 응답특성의 소형 열광학 스위치)

  • 이진표;홍종균;이상선
    • Proceedings of the Optical Society of Korea Conference
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    • 2003.02a
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    • pp.166-167
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    • 2003
  • 실리콘 기판 위에 제작되는 실리카 물질은 낮은 가격, 파이버와의 높은 결합 효율 그리고 고집적화의 장점으로 PLC(Planar Light Circuit)에 사용되고 있다. PLC 광소자 중에서 열 광학 스위치(Thermo-optic switch)는, NⅹN 매트릭스 스위치와 Add/drop multiplexer 등 비교적 낮은 속도를 갖는 광 신호 처리를 위해 중요한 소자이다. 기존의 열 광학 스위치의 경우, 방향성 결합기를 사용한 Mach-Zehnder 구조로써, 위상 제어단에서 두 도파로 간의 광 전력 교환을 막기 위해 방향성 결합기의 양쪽 끝단에에 굽은 도파로를 이용한다. (중략)

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A Study on the Development of Geothermal Energy Using the Hydraulic Fracturing method (수압파쇄법을 이용한 지열에너지 개발에 관한 연구)

  • 이희근
    • Tunnel and Underground Space
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    • v.5 no.4
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    • pp.323-335
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    • 1995
  • 지열에너지 개발의 기본적 개념은 지하 심부의 고온건조암체에 시추공을 이용한 수압파쇄를 실시하여 고온건조암체내에 인공파쇄대를 형성함으로써 유체의 유동회로를 구축하여 지열에너지의 회수를 도모하는 것이다. 본 논문에서는 수압파쇄균열의 발전방향 조절문제와 관련하여, 초고압수 절삭장치를 이용, 수압파쇄공 내에 인공슬롯을 형성하여 수압파쇄를 실시함으로써 균열의 발전방향을 조사하였으며, 수압파쇄에 의한 파쇄대내로의 유체순환실험을 통해 지열수의 유동특성을 규명하였다. 이를 위해 모델에 종균열과 횡균열을 형성시키고 균열내에 주입되는 물의 주입률, 정상류압력, 흐름저항을 조사하고, 이 결과를 이용하여 전산모델링을 수행하였다. 인공절리면에 대한 투수시험에서는 10$0^{\circ}C$까지의 온도변화에 따라 투수계수가 증가하였으며, 봉압 증가에 따라 증가율이 현저히 감소하였다.

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Trends of commercial strain development and spawn industry in mushrooms (버섯의 품종 육성과 종균 산업의 동향)

  • Yoo, Young-Bok;Kong, Won-Sik;Jang, Kab-Yeul;Oh, Se-Jong;Cheong, Jong-Chun;Jhune, Chang-Sung
    • Journal of Mushroom
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    • v.4 no.1
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    • pp.1-32
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    • 2006
  • According to UPOV (International Union for Protection of New Varieties of Plants), mushroom spawn market have to be opened by the year 2009. Number of commercial strains distributed by the year 2005 were 179 of 24 species of edible and medicinal mushrooms. Only nine strains of oyster mushrooms were registered as protected variety, which is not compatible with those recorded in other advanced countries. Meaning of spawn in broad sense contains commercial strains. Development of commercial strains faces two main problems in Korea : One is the complicated genetic patterns and sexuality of mushroom species, and the other is expensive experimental equipments and fruiting body growing houses. Resolution of these problems leads to development of mushroom strains. This could be achieved as follows; genetic resources collection and assessment, molecular characterization of useful genetic characters, development of new commercial strains by hybridization using typical genetic resources, strengthening of breeding research using "Mushroom Breeding Group", management of spawn research company by consortium, foundation of mushroom general industry, promotion of consumption, and upgrade of competition ability for other countries. These points are under discussion.

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Physiological and Genetic Changes by Mixing Culture of Shiitake (표고 배양시 균주 혼입에 따른 생리 및 유전적 변화)

  • Lee, Bong-Hun;Bak, Won-Chull;Kim, Myung-Kil;Ryu, Sun-Hwa;Ryu, Sung-Ryul
    • The Korean Journal of Mycology
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    • v.34 no.2
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    • pp.73-78
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    • 2006
  • Attempts were made to investigate the physiological and genetic changes when two different shiitake (Lentinula edodes) strains are mixed. Mycelial growth of KFRI 180 strain and KFRI 1 strain were investigated 82 mm and 80 mm, respectively. Concerning the weight loss percentage of medium, KFRI 1 strain decreased 2.4% and KFRI 180 strain 1.6%. Plug-shaped spawn had no-problem to incubate and there were no differences among the ratios of mixture. Also, conditions of plug-shaped spawns were similar, When the isolated mycelia from plugshaped spawns was incubated again, KFRI 1 50%-KFRI 180 50% showed decreased growth of mycelia compared with other treatments. The same results were obtained from test tubes filled with sawdust. When surface of spawn bottles were observed, KFRI 1 50%-KFRI 180 50% showed spots, but other treatments were not different from KFRI 1 and KFRI 180. Test was made to confirm the strains by confrontation culture. The mixture of two strains was proved to be KFRI 1 regardless the ratios of mixture. However, by the RAPD primer analysis, when KFRI 1 was mixed with KFRI 180, KFRI 180 was stronger. Thus, the confrontation line on PDA was different from the bands analysis by primers. Attempts were made whether the fruit-bodies were made at the generating condition of spawn bottles. The results were that KFRI 1 100%, KFRI 1 90%-KFRI 180 10%, KFRI 1 80%-KFRI 180 20%, KFRI 1 50%-KFRI 180 50% treatment showed fruit-body formation. The shape of fruit-body was deformed, but the gill was made normally.