Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
권호 표지
DOI QR코드

DOI QR Code

Development of a spawning method using liquid inoculum of Agaricus bisporus

양송이 액체접종원을 이용한 종균 제조 방법 개발

  • Oh, Youn-Lee (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Jang, Kab-Yeul (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Oh, Min Ji (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Im, Ji-Hoon (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
  • 오연이 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 장갑열 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 오민지 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 임지훈 (농촌진흥청 국립원예특작과학원 버섯과)
  • Received : 2021.03.12
  • Accepted : 2021.06.16
  • Published : 2021.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Currently, the spawn of the mushroom Agarcus bisporus is produced by a method developed in the 1980s, and anew spawning method needs to be developed to improve the quality of the spawn. In this study, the condition for a maximum mycelium weight(5.92±0.52 g/L) was shaking culture (24 hours/day) at 24℃ and 120 rpm in CDB (compost dextrose broth). Based on this, the ventilated liquid culture method (2.5 L/min) was cultured for 10 days. This method was appropriate, andwhen the inoculum was cultured at 50 g/mL for about 10 days, it was cultured well without agglomeration and shaking of seed.

현재 양송이 종균은 1980년대 개발된 방법으로 제조되고 있어, 양송이 종균 품질 향상을 위한 새로운 제조 방법을 개발하였다. 그 결과 균사체량이 5.92±0.52 g/L로 가장 많이 배양된 CDB(compost dextrose broth) 배지에서 24℃에서 120 rpm으로 진탕 배양(24시간/일) 하는 통기식(2.5 L/min) 액체배양법으로 배양기간 10일이 액체 접종원 사용으로 양호했으며, 양송이 밀 배지 종균 생산은 밀 배지 50 g당 액체 접종원 1ml을 적정 접종량으로 하여 10일 배양하면 종균의 뭉침과 흔드는 작업없이 적절하게 배양되었음을 보고한다.

Keywords

Acknowledgement

본 결과물은 농촌진흥청 '수출용버섯 종균제조 기술개발(PJ01341101)' 주관과제의 예산을 지원받았습니다.

References

  1. Cha DY, Yu CH, Kim GP. 1989. Current mushroom cultivation technology. 67-69.
  2. Cheong JC, Kim HG, Kim GP. 1996. Development of liquid spawn for manufacturing bottle mushroom medium. Agricultural Science and Technology Institute test research project report (Ministry of Biological Resources): 635-644.
  3. Choi SJ, Kim SJ, Han YH. 2004. Physiological characteristics and optimized culture condition of the mycelia of Inonotus mikadoi. Korean J Microbiol 40: 100-103.
  4. Friel MT, McLoughlin AJ. 2000. Production of a liquid inoculum/spawn of Agaricus bisporus. Biotechnol Lett 22: 351-354. https://doi.org/10.1023/A:1005616516646
  5. Kim YD, Kim YH. 2009. Method of culturing Agaricus bisporus mycelium and medium for culturing the same. Patent WO2007139322A1. PCT/KR2007/002555.
  6. MAFRA. 2020. Ministry of Agriculture, Food and Rural Affairs 2019. 64-67.
  7. Oh YL, Jang, KY, Kong WS, Shin, PG, Kim ES, Oh MJ, Choi IG. 2015. Development of a new brown button mushroom cultivar 'Hogam'. J Mushrooms 13: 237-242. https://doi.org/10.14480/JM.2015.13.3.237
  8. Royse DJ, Baars J, Tan Q. 2017. Current Overview of Mushroom Production in the World. Edible and Medicinal Mushrooms: Technology and Applications 5-13.
  9. Sung GH., Shrestha B, Park KB, Sung JM. 2010. Cultural Characteristics of Shimizuomyces paradoxus Collected from Korea. Mycobiology 38: 189-194. https://doi.org/10.4489/MYCO.2010.38.3.189