• Title/Summary/Keyword: 자실체

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Isolation of Myxobacteria from Soil and RFLP Analysis of 16S rDNA Fragments. (토양으로부터 Myxobacteria의 분리 및 165 rDNA RFLP분석)

  • 김수광;최병현;김종균;이병규;강희일
    • Korean Journal of Microbiology
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    • v.39 no.3
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    • pp.187-191
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    • 2003
  • In an attempt to isolate myxobacteria from soil samples, we isolated swarm and fruiting body forming bacteria that have bacteriolytic activity on Coli-spot agar plate. For the classification of myxobacteria, 16S rDNA RFLP patterns were analyzed. Amplified 16S rDNAs of myxobacteria type strains (Family I, II, III and IV), negative control strains and soil-isolates were restricted with HaeIII, EcoRI and EcoRV, respectively. We found that the soil-isolates belongs to myxobacteria Family I, II, III.

Note on Tuber aestivum subsp. uncinatum Newly Recorded in Korea (한국산 미기록종 여름갈고리덩이버섯(Tuber aestivum subsp. uncinatum)에 관하여)

  • Shin, Kwang-Soo;Park, Jin-Seo;Yoshimi, Syoichi
    • The Korean Journal of Mycology
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    • v.23 no.1 s.72
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    • pp.10-13
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    • 1995
  • The hypogeous ascomycete, Tuber aestivum subsp. uncinatum was isolated and identified for the first time in Korea from Quercus forest soil in Kyeryong-san National Park in August, 1992. The fungus is reported here with Korean name and English description. The specimen cited is deposited in herbarium of Department of Microbiology, Taejon University.

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Chemical Constituents from the Fruit Bodies of Lentinula edodes (표고(Lentinula edodes) 자실체의 추출성분)

  • Lee, Hak-Ju;Yoon, Kab-Hee;Bak, Won-Chull
    • Journal of the Korean Wood Science and Technology
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    • v.31 no.2
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    • pp.24-30
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    • 2003
  • Three nucleotides, one amide, acid and steroid, were isolated from the fruit bodies of Lentinula edodes. The structures were determined as adenosine (9-𝛽-D-ribofuranosyladenine), uridine (1-𝛽-D-ribofuranosyluracil), uracil (2, 4-pyrimidinedione), nicotinamide, p-hydroxybenzoic acid and ergosterol (ergosta-5, 7, 22-triene-3𝛽-ol), respectively, on the basis of spectroscopic data and chemical correlations.

Chemical Constituents from the Fruit Bodies of Tricholoma matsutake (송이(Tricholoma matsutake) 자실체의 화학성분)

  • Lee, Hak-Ju;Choi, Yun-Jeong;Ka, Kang-Hyeon;Bak, Won-Chul
    • Journal of the Korean Wood Science and Technology
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    • v.31 no.4
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    • pp.63-70
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    • 2003
  • One alkaloid derivatives, one amide and two steroids were isolated from the fruit bodies of Tricholoma matsutake. The structures were determinded as adenosine, methyl trans-cinnamate, ergosterol and ergosta-4, 6, 8 (14), 22-tetraen-3-one, respectively, on the basis of spectroscopic data.

Intra- and Intermatings among Strains of Pleurotus ostreatus and P. florida (느타리버섯과 사철느타리버섯의 균주간(菌株間) 및 종간(種間) 교배(交配))

  • Go, Seung-Joo;Cha, Dong-Yeul;Park, Young-Hwan
    • The Korean Journal of Mycology
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    • v.9 no.1
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    • pp.13-18
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    • 1981
  • This study was performed to investigate the high yielding potential of Pleurotus ostreatus (Fr.) Quel derived from intermating and intramating of three strains: Local strain, Nonggi 201, and Pleurotus florida Eger. All the strains tested were tetrapola mating systems, and hybridable. Intermating system resulted in more fresh sporophores than those from intramating. The highest yield of fresh sporophores of P. ostreatus was obtained from Nonggi 201 crossed with P. florida, when cultivated on the rice straw substrates.

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Cultural conditions and morphological characteristics of Portobella, Agaricus brunescens (큰양송이 Portobella 균주의 배양조건 및 형태적 특성)

  • Chi, Jeong-Hyun;Lee, Jae-Hong;Kim, Hee-Dong;Kim, Young-Ho
    • Journal of Mushroom
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    • v.1 no.1
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    • pp.21-27
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    • 2003
  • The compost dextrose(CD) medium was selected a an optimum liquid culture media for Portobella(Agaricus brunescens). The optimal conditions for the mycelial growth of Agaricus brunescens were 25 and pH 7.0. Sucrose and soluble starch were favorable to mycelial growth. Malt extract and ammonium nitrate appeared to be more favorable than the other kinds of nitrogen source. The optimum C/N ratio as about 10.When the collected Portobella trains were compared with growth characteristics, KME 49007 appeared the highest of $44.6kg/3.3m^2$.

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Genetic Variability of Flammulina velutipes Monosporous Isolates (팽나무버섯(Flammulina velutipes) 단포자 분리주의 유전적 변이성)

  • Kong, Won-Sik;Kim, Dong-Hyun;Kim, Young-Ho;Kim, Kyoung-Soo;You, Chang-Hyun;Byun, Myung-Ok;Kim, Kwang-Ho
    • The Korean Journal of Mycology
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    • v.25 no.2 s.81
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    • pp.111-120
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    • 1997
  • The characteristics of monosporous isolates of winter mushroom [Flammulina velutipes (Curt. ex Fr.)] were investigated to obtain useful breeding materials. Within monokaryons from cultivars which have a white colored fruitbody showed narrow genetic variation, while domestic strains which have a brown colored fruitbody showed wide variation. The mating type of the white strains was A1A2B1B2 genotype, but that of the domestic brown strains were A3A4B3B4. In intra-crossing, the genetic stability of dikaryons mated by monokaryons from white strain was less than that of parents. While in brown strain, dikaryons with high yield and color variation were obtained.

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Cultural and Morphological characteristics of fruit body of genus Agrocybe on Artificial Cultivation in Summer (볏짚버섯속(Agrocybe) 보존균주의 여름철 인공재배시 재배 및 형태적 특성)

  • Cheong, Jong-Chun;Seok, Dong-Kwon;Kim, Seung-Hwan;Jhune, Chang-Sung;Lee, Chan-Jung
    • Journal of Mushroom
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    • v.7 no.1
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    • pp.1-8
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    • 2009
  • Thirty strains of genus Agrocybe which had been kept in National Institute of Horticultural and Herbal Science, were tested for the formation of fruiting body. Nineteen strains of collected genus Agrocybe were formed fruiting body at bottle culture of sawdust medium. Five strains (including ASI 19003) and thirteen strains (including ASI 19007) could be grouped as A. cylindracea and A. chaxingu. Although ASI 19008 showed the formation of fruiting body, morphological characteristics were significantly different from the two groups. Cultural period of ASI 19003 strain at sawdust substrates was 29 days and ASI 19007 train was 30 days in liquid spawn inoculation. The yield and quality of ASI 19003 strain was excellent in the spring, fall, and winter, whereas ASI 19007 strain was excellent in the summer. Accordingly, these cultivar might be contribute to farmers' income by stable year-round production if using the season-oriented strain.

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Optimum mixing rate of used media for saving the production cost of Flammulina velutipes (팽이버섯(Flammulina velutipes) 생산비 절감을 위한 폐배지 적정 혼합비율)

  • Jung, Kyung Ju;Choi, Duck Soo;Bang, Geuk Pil;Chung, Ki Chul
    • Journal of Mushroom
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    • v.7 no.1
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    • pp.22-26
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    • 2009
  • These experiments were conducted to find the optimum mixing rate of used media for saving the production cost of Flammulina velutipes. The materials for media formation was used needle-leaf tree sawdust, media that finished 1th cultivation of F. velutipes, corncob meal, and rice bran, wheat bran as nutrition source. We inoculated the F. velutipes in 14 kinds of different media types and checked the spawn growth speed, fruit body quality and quantity. Two nutrition agents, which is rice bran and wheat bran, did not affected the incubation period, but the effective stem number, quality and quantity of fruit body was better at rice bran than wheat bran. The quality of fruit body produced at mixed 20% of used media (needle-leaf tree sawdust 60% + used media 20% + rice bran 20%) was similar to control plot (needle-leaf tree sawdust 80% + rice bran 20%), but the yield was improved 10% than control plot 130g. According as the used-media mixing amount increases, quality and quantity of fruit body became low remarkably. Therefore, the optimum mixing amount of used-media was 20% and it increased 10% of fruitbody yield.

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Effect of schizandra berry dregs and rice bran treatment on γ-aminobutyric acid (GABA) content enhancement in Pleurotus ostreatus (오미자박과 미강 첨가배지가 느타리버섯 자실체의 γ-aminobutyric acid(GABA) 함량에 미치는 효과)

  • Jeoung, Yun-Kyeoung;Kim, Jeong-Han;Baek, Il-Sun;Kang, Young Ju;Chi, Jeong-Hyun
    • Journal of Mushroom
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    • v.15 no.2
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    • pp.88-93
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    • 2017
  • This study was carried out to establish a cultivation technique for increasing the ${\gamma}$-aminobutyric acid (GABA) content in the fruit body of mushrooms by adding processed by-products. For the oyster mushroom 'Heucktari', addition of green tea powder, sea tangle powder, and green tea dregs resulted in very poor primordia formation, fruit body growth, and increased GABA. However, addition of 10% schizandra berry dregs and 1% rice bran to the basal substrate induced 100% and 10% increases, in GABA content in the fruit bodies compared to the control treatment without by-product, respectively. In addition, fruit body growth and primordia formation were greatly increased by these treatments. Therefore, GABA content was increased when the substrate was prepared by mixing an appropriate amount of schizandra berry dregs and rice bran.