Genetic Variability of Flammulina velutipes Monosporous Isolates

팽나무버섯(Flammulina velutipes) 단포자 분리주의 유전적 변이성

  • Published : 1997.06.30

Abstract

The characteristics of monosporous isolates of winter mushroom [Flammulina velutipes (Curt. ex Fr.)] were investigated to obtain useful breeding materials. Within monokaryons from cultivars which have a white colored fruitbody showed narrow genetic variation, while domestic strains which have a brown colored fruitbody showed wide variation. The mating type of the white strains was A1A2B1B2 genotype, but that of the domestic brown strains were A3A4B3B4. In intra-crossing, the genetic stability of dikaryons mated by monokaryons from white strain was less than that of parents. While in brown strain, dikaryons with high yield and color variation were obtained.

팽나무버섯[Flammulina velutipes(Curt. ex Fr.) Singer]의 새로운 우량균주를 육성하고자 단핵균주를 분리하여 이들의 특성을 조사하고 계통내 교배 시험한 결과 백색 재배종에서 분리된 단핵균주는 모균주에 비해 균사생장의 변이가 적고, 균총의 색이 모두 백색이며 RAPD 밴드로 본 변이가 적었던 반면, 갈색 자생종에서 분리된 단핵균주는 균총의 색이 백색, 갈색, 연갈색으로 분리되고 RAPD 밴드에 다양한 변이를 보였다. 또한 백색 재배종의 교배형은 A1A2B1B2 교배형이었으나, 갈색 야생종의 교배형은 백색종과 교배형이 다르게 나타나 A3A4B3B4교배형으로 결정하였다. 백색 재배종에서 분리한 단핵균주간 자식교배에 의하여 육성한 이핵균주는 모본보다 자실체 수량이 적고 수량의 변이 폭이 컸다. 갈색 자생종에서 분리한 단핵균주를 서로 교배한 결과 자실체의 색깔에 변이를 나타내었는데 백색 자실체를 형성하는 교배균주 중에는 모본보다 수량이 많은 이핵균주도 있었다. 따라서 갈색 자생종의 자식교배로 현재 재배되는 순백계와는 다른 새로운 백색계통을 육성할 수 있었다.

Keywords

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