• Korean Journal of Microbiology
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• v.52 no.1
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• pp.10-17
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• 2016

Enzyme immunoassay to analyze specific binding activity of antibody to antigen uses horseradish peroxidase (HRP) or alkaline phosphatase (AP). Chemical methods are usually used for coupling of these enzymes to antibody, which is complicated and random cross-linking process. As results, it causes decreases or loss of functional activity of either antibody or enzyme. In addition, most enzyme assays use secondary antibody to detect antigen binding activity of primary antibody. Enzymes coupled to secondary antibody provide a binding signal by substrate-based color development, suggesting secondary antibody is required in enzyme immunoassay. Additional incubation time for binding of secondary antibody should also be necessary. More importantly, non-specific binding activity caused by secondary antibody should also be eliminated. In this study, we cloned AP isolated from Escherichia coli (E. coli) chromosome by PCR and fused to) hAY4 single-chain variable domain fragment (ScFv) specific to death receptor (DR4) which is a receptor for tumor necrosis factor ${\alpha}$ related apoptosis induced ligand (TRAIL). hAY4 ScFv-AP expressed in E. coli showed 73.8 kDa as a monomer in SDS-PAGE. However, this fusion protein shown in size-exclusion chromatography (SEC) exhibited 147.6 kDa as a dimer confirming that natural dimerization of AP by non-covalent association induced ScFv-AP dimerization. In several immunoassay such as ELISA, Western blot and immunocytochemistry, it showed antigen binding activity by color development of substrates catalyzed by AP directly fused to primary hAY4 ScFv without secondary antibody. In summary, hAY4 ScFv-AP fusion protein was successfully purified as a soluble dimeric form in E. coli and showed antigen binding activity in several immunoassays without addition of secondary antibody which sometimes causes time-consuming, expensive and non-specific false binding.

• The Korean Journal of Applied Statistics
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• v.14 no.2
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• pp.401-413
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• 2001

이차원 분할자료에 대해서 행과 열의 관계를 저차원상에 시각적으로 표현하는 탐색적대응분석에 대하여 붓스트랩방법의 사용가능성을 살펴보았다. 기존의 탐색적 면만이 강조되어 왔던 대응분석에서 좌표점의 변이와 좌표점간의 거리에 대한 통계적 추론을 붓스트랩방법으로 해결할 수 있음을 보이고 또한 좌표축의 설명력에 대하여 붓스트랩신뢰구간의 포함확률의 일치성을 모의실험을 통해 제시하였다.

• Bulletin of Food Technology
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• v.18 no.2
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• pp.3-13
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• 2005

인삼의 효능 및 유효성분에 관한 수많은 문헌 및 연구사례가 보고 되어 있음에도 불구하고 인삼의 주성분인 ginsenosides의 합성경로나 그와 관련된 유전인자에 대하여서는 거의 밝혀져 있지 않다. 인삼의 생리적 특성규명 및 ginsenosides의 생합성 경로를 밝히기 위한 한 방법으로써 프로테옴 분석이 시도되었다. 본지에서는 고려인삼 (Panax ginseng C.A. Meyer)을 대상으로 이루어진 최근의 프로테옴 분석 및 이차 대사산물의 생산과 연관된 유전인자를 찾기 위하여 이루어진 인삼의 기능유전체 분석을 소개하고자 한다.

• Proceedings of the KIPE Conference
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• 2010.07a
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• pp.198-199
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• 2010

본 논문에서는 전기자동차의 구동에 핵심적인 역할을 하는 이차전지의 충전 방식 중 간단히 구현가능하며 성능이 뛰어난 정전류 정전압 알고리즘과 펄스 충전 알고리즘을 이론적으로 자세히 분석하였으며, 각각의 경우의 충전전류, 충전전압의 특성을 분석하였다. 또한 충전 시퀀스를 정리하였고 배터리 사고방지를 위한 $V_{th}$의 최대 설정치를 분석하였다.

• Proceedings of the Korean Nuclear Society Conference
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• 1997.10a
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• pp.79-84
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• 1997

동특성 분석 코드 시스템 PANBOX2는 시간에 대한 미분을 Implicit Euler 방법을 사용하여 근사한다. 이 경우 Local Truncation Error는 중성자속의 이차 미분에 비례한다. Time-Step-Doubling 기법을 이용하여 Local Truncation Error의 근사치를 구하고 이를 이용하여 Time Step Size를 조절해 주는 방법을 동특성 분석 코드 시스템 PANBOX2에 도입하였다. LRA와 NEACRP 제어봉 인출사고 검증문제에 대한 분석 결과, PANBOX2 시스템의 기존 방법에 비해 효과적으로 Time Step을 제어하였으며 보다 정확한 결과를 얻을 수 있었다.

• Korean Journal of Plant Resources
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• v.35 no.5
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• pp.586-593
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• 2022

Soybean (Glycine max (L.) Merr.) is a high-protein oilseed crop, cultivated worldwide. Soybean seeds are abundant in various secondary metabolites with physiologically active. Mature seeds of 25 soybean cultivars with various morphological and physiological characteristics analyzed for secondary metabolites, such as carotenoid, isoflavone, and soyasaponin. These secondary metabolites showed various content by genotype in 25 cultivars. Total carotenoid content ranged from 1.23 to 33.78 mg/g and three cultivars, such as IT177645, PI90763 and IT234975 with black seed coat showed high levels of total carotenoid. Total isoflavones content ranged from 20.28 to 276.35 mg/100g and were detected high levels in Savoy, PI90763 and KLG16001. In addition, total soyasaponins content ranged from 33.12 to 246 mg/100g and were detected high levels in PI90763, PI86490 and IT234975. The PI90763 was showed abundant content in all of the carotenoid, isoflavones and soyasaponins. These results could be valuable information for the development of new soybean cultivars and regulation of secondary metabolites biosynthesis in soybean.

• Journal of the Korean School Mathematics Society
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• v.16 no.4
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• pp.883-898
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• 2013

The purpose of this study is to examine that high school students recognize mathematical situation when they are requested for changing identical mathematics situations into different situations. The results of the study are followings. First, percentage of correct answers to the questions of turning equal mathematical situation into function is higher than the one of turning equal mathematical situation into equation and inequality. As a result of individual interview for comprehensibility of the students on these relations, it is found that if degree goes up and there is different expressions of questionaries although mathematical situation is identical, it affects comprehensibility of the subjects. Second, we found that they cannot understand identical mathematics situations because they have trouble in drawing graph or applying to get the answer while many students understand a point of intersection on the graph as a correct answer. Third, As a result of individual interview for comprehensibility of the students on relation between equation, inequality and function, we found that students manage to get correct answer even without perfect comprehensibility on this relation.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.5B
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• pp.539-549
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• 2006

The performance of a pontoon-type floating breakwater (FB) is investigated numerically with the use of a second-order time domain model. The model has been developed based on potential theory, perturbation theory and boundary element method. This study is focused on the effects of weakly nonlinear wave on the hydrodynamic characteristics of the FB. Hydrodynamic forces, motion responses, surface elevation, and wave transmission coefficient around the floating breakwater are evaluated for various wave and geometric parameters. It is shown that the second-order wave component is of significant importance in calculating magnitudes of the hydrodynamic forces, mooring forces and the maximum response of a structure. The weak non-linearity of incident waves, however, can have little influence on the efficiency of the FB. From numerical simulations, the ratio of draft and depth, the relationship of wave number and width are presented for providing an effective means of reducing wave energy.

• Journal of Korean Academy of Nursing
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• v.54 no.1
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• pp.18-31
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• 2024

Purpose: This study aimed to investigate the impact of anthropometric indices of obesity (body mass index [BMI], waist circumference, waist hip ratio, and body fat percentage) on the incidence of hypertension in adults with prehypertension. Methods: A longitudinal study design using secondary data form the Korean Genome and Epidemiology Study was employed. The study included 1,838 adults with prehypertension tracked every two years from 2001 to 2018. Statistical analyses, including frequency assessments, number of cases per 1,000 person-years, log-rank tests, Kaplan-Meier curves, and Cox's proportional hazards regression, were conducted using SPSS version 25. Results: Over the observation period (15,783.6 person-years), 1,136 individuals developed hypertension. The incidence of hypertension was significantly higher in the obesity groups defined by BMI (hazard ratio [HR] = 1.33), waist circumference (HR = 1.34), waist hip ratio (HR = 1.29), and body fat percentage (HR = 1.31) compared to the non-obese group. These findings indicate an increased risk of hypertension associated with obesity as measured by these indices. Conclusion: The study underscores the importance of avoiding obesity to prevent hypertension in individuals with prehypertension. Specifically, BMI, waist circumference, waist hip circumference, and body fat percentage were identified as significant risk factors for hypertension. The results suggest the need for individualized weight control interventions, emphasizing the role of health professionals in addressing the heightened hypertension risk in this population.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.32 no.4
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• pp.80-87
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• 2004

To understand the each performance parameter correlation of flight type liquid-propellant rocket engine for KSR-III(Korea Sounding Rocket-III), the analysis of engine stand-alone combustion test results was carried out. Considering the variation of ablative material combustion chamber caused by erosion, linear regression analysis that ignores oxidizer/fuel ratio effect and two-variable 2nd-order polynomial regression analysis that considers oxidizer/fuel ratio change were performed. It can be described that linear regression analysis is simple and very practical method, and can predict the performance within 1% error inside analyzed region. And two-variable 2nd-order polynomial regression analysis can predict with very high accuracy inside region and shows that KSR-III engine's optimum oxidizer/fuel ratio for thrust(or specific impulse) is 2.22 and that for combustion chamber pressure(or characteristic velocity) is 2.17.