Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Analysis of Secondary Metabolites in Various Cultivars of Soybean (Glycine max (L.) Merr.)

다양한 콩 자원들의 이차대사물질 함량 분석

  • Seo, Mi-Suk (Crop Foundation Research Division, National Institute of Crop Science) ;
  • Park, Gyu Tae (Crop Foundation Research Division, National Institute of Crop Science) ;
  • Kim, Hyun Young (Crop Foundation Research Division, National Institute of Crop Science) ;
  • Lee, Sang-Beom (Crop Foundation Research Division, National Institute of Crop Science) ;
  • Kim, Yu-na (Crop Foundation Research Division, National Institute of Crop Science) ;
  • Park, Soo-Kwon (Crop Foundation Research Division, National Institute of Crop Science) ;
  • Kim, Dool-Yi (Crop Foundation Research Division, National Institute of Crop Science) ;
  • Mun, Jung Kyung (Crop Foundation Research Division, National Institute of Crop Science)
  • 서미숙 (국립식량과학원 작물기초기반과) ;
  • 박규태 (국립식량과학원 작물기초기반과) ;
  • 김현영 (국립식량과학원 작물기초기반과) ;
  • 이상범 (국립식량과학원 작물기초기반과) ;
  • 김유나 (국립식량과학원 작물기초기반과) ;
  • 박수권 (국립식량과학원 작물기초기반과) ;
  • 김둘이 (국립식량과학원 작물기초기반과) ;
  • 문중경 (국립식량과학원 작물기초기반과)
  • Received : 2022.09.01
  • Accepted : 2022.09.13
  • Published : 2022.10.01
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Abstract

Soybean (Glycine max (L.) Merr.) is a high-protein oilseed crop, cultivated worldwide. Soybean seeds are abundant in various secondary metabolites with physiologically active. Mature seeds of 25 soybean cultivars with various morphological and physiological characteristics analyzed for secondary metabolites, such as carotenoid, isoflavone, and soyasaponin. These secondary metabolites showed various content by genotype in 25 cultivars. Total carotenoid content ranged from 1.23 to 33.78 mg/g and three cultivars, such as IT177645, PI90763 and IT234975 with black seed coat showed high levels of total carotenoid. Total isoflavones content ranged from 20.28 to 276.35 mg/100g and were detected high levels in Savoy, PI90763 and KLG16001. In addition, total soyasaponins content ranged from 33.12 to 246 mg/100g and were detected high levels in PI90763, PI86490 and IT234975. The PI90763 was showed abundant content in all of the carotenoid, isoflavones and soyasaponins. These results could be valuable information for the development of new soybean cultivars and regulation of secondary metabolites biosynthesis in soybean.

콩(Glycine max (L.) Merr.)은 전세계적으로 널리 재배되고 있는 고단백 유지 작물로서 다양한 생리활성을 가지는 이차대사산물을 함유하고 있다. 본 연구에서는 콩 25개 자원들을 대상으로 카로티노이드, 이소플라본, 그리고 소야사포닌 함량을 분석하였다. 총 카로티노이드 함량을 분석한 결과, 1.23~33.78 ㎍/g의 분포를 보였고, IT177645, PI90763, 그리고 IT234975과 같은 검은 종피색을 가진 자원들에서 가장 높은 카로티노이드 함량이 관찰되었다. 이소플라본 함량은 Savoy, PI90763, KLG16001에서 높게 확인되었고, 소야사포닌은 PI90763, PI86490, 그리고 IT234975에서 가장 높은 함량을 보였다. PI90763은 카로티노이드, 이소플라본, 그리고 소야사포닌 3종 모두를 고농도로 함유한 자원으로 확인되었다. 본 실험의 결과, 다양한 이차대사산물의 고함량 자원들은 고기능성 품종 개발을 위한 육종 소재 및 이차대사산물의 생합성 관련 연구를 위한 기초자료로써 활용이 가능할 것이다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 국립식량과학원 어젠다 사업(과제번호: PJ014954022022)의 지원에 의하여 수행되었습니다. 연구수행을 위하여 실험적 지원을 해주신 국립식량과학원 작물기초기반과 강현애, 송지연 선생님께 감사드립니다.

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