• Journal of the Society of Cosmetic Scientists of Korea
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• v.43 no.4
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• pp.365-371
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• 2017

This study was carried out to investigate the effect of lacquer tree (Rhus verniciflua) extract on ganodermanondiol (GN), tyrosinase and melanin biosynthesis inhibitor, biosynthesis in Ganoderma lucidum mycelia. In HPLC analysis, GN contents were significantly increased in G. lucidum mycelial extracts supplemented with of 1, 5, 10, and 15% lacquer tree extracts (LTE). In addition, G. lucidum mycelial extracts supplemented with LTEs which had no cytotoxicity activity against B16F10 cells, significantly inhibited melanogenesis in B16F10 cells. GN biosynthesis was facilitated by LTE. Taken together, we propose that G. lucidum mycelial extracts supplemented with LTE can be used as an effective ingredient of skin care products in the future.

• Journal of Mushroom
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• v.11 no.3
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• pp.164-170
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• 2013

This study was carried out to compare the medicinal effects of various fruiting body of Ganoderma species and Cordyceps militaris, Phelinus linteus extracts. ${\beta}$-glucan and polyphenol are useful ingredient in mushrooms and they were known to have antioxidant activity. We analyzed ${\beta}$-glucan and polyphenol contents of fruiting body of Ganoderma spp., Cordyceps militaris, and Phellinus linteus. Most Ganoderma spp. exhibited ${\beta}$-glucan contents of 15 to 20%. Cordyceps militalis showed the highest ${\beta}$-glucan level of 25%. Interestingly, eight strains of Ganoderma spp. was analyzed to have higher contents of ${\beta}$-glucan than Phelinus linteus. Polyphenol contents was measured after extraction with different solvents. (D.W., 70% EtOH, 80% MeOH) The level of polyphenol in ASI 7020 strain was at maximum in the water extraction and ASI 7086 showed the highest level in the 70% EtOH extraction. The amounts of polyphenol in strain ASI 7113 was at maximum in the 80% MeOH extraction.

• Journal of Mushroom
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• v.13 no.3
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• pp.170-174
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• 2015

Mushroom is known for anti-inflammatory and anti-oxidative potential. This study provides evidence that the inhibitory effect of mushroom on the expression of pro-inflammatory cytokines in human keratinocytes, HaCaT cells. To define the underlying mechanisms of action, tumor necrosis factor${\alpha}$/$IFN{\gamma}$-activated human keratinocytes model was used. Mushroom significantly inhibited the expression of cytokines in HaCaT cells. Taken together, the results demonstrate that mushroom inhibited inflammtion, suggesting that mushroom (DW extract: Grifola frondosa Cordyceps militaris), (Ethanol extract: Ganoderma lucidum, Lentinus edodes, Cordyceps militaris, Flammulina velutipes) might be a candidate for the treatment of skin inflammation.

• Proceedings of the Korean Journal of Food and Nutrition Conference
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• 2001.12a
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• pp.130-130
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• 2001

버섯은 특유의 향과 풍미 뿐 아니라 단백질, 다당류, 비타민, 무기질 등을 고루 함유란 저칼로리 영양식품으로서 가치가 높고, 최근에는 생체방어, 항상성 유지, 질병의 회복뿐만 아니라 암, 뇌졸중, 심장병 등의 성인병에 대한 예방과 개선효과가 있는 것으로 알려져 기능성 식품소재로서 활용가치가 높아지고 있다. 그러나 버섯류는 대부분 생체 또는 건조품으로 소비되고 있으며 재배기술이 개선되어 점진적으로 생산량이 증가되나. 수요가 이를 따르지 못해 계절적 공급과잉으로 가격파동이 우려된다. 따라서 출하조절과 버섯 수요를 확대를 위하여 영지, 표고, 눈꽃동충하초 그리고 번데기동충하초를 이용한 버섯젤리의 제조와 제품의 색도, 물성 및 기호도를 조사 비교하였다. 버섯 젤리 제조를 위한 추출액의 적정혼합비율을 선발한 결과, 영지버섯 젤리는 버섯추출액 85%, 대추추출액 10%, 황기추출액 5%를 혼합했을 때, 표고버섯 젤리는 버섯추출액 80%, 대추추출액 10%, 감초추출액 5%, 오미자추출액 5%를 혼합했을 때, 눈꽃동충하초와 번데기동충하초 젤리는 버섯추출액 85%, 대추추출액 10%, 감초추출액 5%를 혼합했을 때 가장 우수하였다. 젤화제 종류별로 버섯 추출액에 대한 응고 효과는 모든 버섯에 대해 carrageenan이 가장 효과적이었다. 버섯 젤리의 색도는 carrageenan 첨가량에 따라 큰 차이가 없었고, hardness, gumminess, chewiness는 증가하는 경향이었다. 관능은 젤화가 완전히 이루어지면서 hardness가 낮은 carrageenan 0.6% 첨가가 가장 우수하였다.

• Korean Journal of Food Science and Technology
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• v.29 no.2
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• pp.194-197
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• 1997

Dopamine ${\beta}-hydroxylase\;(DBH)$ catalyses the enzymatic reaction of dopamine to norepinephrine. For the purpose of screening DBH inhibitory activity from edible mushrooms, Ganoderma lucidum, Agaricus bisporus and Lentinus edodes were examined by tracing inhibitory activities against bovine adrenal DBH, utilizing tyramine as a substrate. Among the three edible mushrooms tested, Ganoderma lucidum showed potent enzyme inhibitory activilies above 100% against DBH in chloroform fraction. Lentinus edodes and Agaricus bisporus showed inhibitory activities in ethylacetate fraction on 79.7% and 64.7%, respectively. Each solvent fraction of these mushrooms were assessed in the aspects of their inhibitory activities against DBH, and their $IC_{50}$ values were calculated. $IC_{50}$ value of chloroform fraction of Ganoderma lucidum was $1.60{\times}10^{-4}\;g$, and those of ethylacetate fractions of Agaricus bisporus and Lentinus edodes were $5.50{\times}10^{-4}\;g\;and\;2.35{\times}10^{-4}\;g$, respectively.

• Korean Journal of Food Science and Technology
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• v.28 no.5
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• pp.947-952
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• 1996

The objective of this study was to evaluate the effect of mushrooms on the growth of intestinal lactic acid bacteria. Bifidobacterium breve and the total intestinal flora of human and rats were inoculated in the general anaerobic medium which contained each mushroom water extract. Except Pleurotus ostreatus and Flammulina velutipes, the mushroom extracts induced the growth of lactic acid bacteria by decreasing pH of the broth. The pH decreasing effect was excellent especially with Lentinus edodes, Agarocus bisporus and Coriorus versicolor. This effect was due to the increase in the number of Bifidobacterium in the intestinal bacterid. This growth of lactic acid bacteria effectively inhibited the bacterial enzymes, ${\beta}-glucosidase,\;{\beta}-glucuronidase$ and tryptophanase, of intestinal bactetria.

• Journal of Mushroom
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• v.21 no.3
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• pp.185-189
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• 2023

This study was conducted to develop a renewable and sustainable bio-material to replace polystyrene (EPS) in fungal-mycelium-based composite using agricultural by-products. Four mushrooms (Ganoderma lucidum, Fomitella fraxinea, Phellinus linteus, and Schizophyllum commune) were cultured in an oak sawdust plus rice bran substrate to select the mushroom with the best growth. The mycelia of G. lucidum showed the best growth. To investigate the optimal mixing ratio with spent mushroom substrate (SM) and oak sawdust (OS), samples were prepared by mixing SM and OS at ratios of 50%:50%, 60%:40%, and 80%:20% (w/w). Each substrate was then inoculated with G. lucidum. G. lucidum showed the best mycelial growth of 140.0 mm in the substrate with SM and OS mixed at a 60%:40% ratio. It was also found that the substrate with SM and OS mixed at a 60%:40% ratio had the best handling properties. The compressive strength of mycelial materials inoculated with G. lucidum was in the range of 300-302 kgf mm^-1, and the materials were four times stronger than polystyrene materials. These results indicate that substrates comprising spent mushroom substrate mixed with oak sawdust can be successfully upcycled to mycelium-based composite materials using G. lucidum. This represents a sustainable approach.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.112-112
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• 1995

간장 손상시에는 여러 혈청의 효소 활성과 함께 혈청 $\beta$-glucuronidase의 활성도 증가한다는 것이 보고되었으나 심한 간부전이나 간암의 경우 이들의 활성은 오히려 정상치보다 감소하는 것으로 나타났다. Silymarin은 간장 보호효과로 이미 임상에 널리 사용되고 있는 약물로서 김 등에 의해 silymarin이 장내세균의 $\beta$-glucuronidase와 간장의 $\beta$-glucuronidase의 활성을 억제한다는 것이 보고되었다. 이에 연자 둥은 $\beta$-glucuronidase의 저해 효과가 관찰된 영지버섯온 유기용매로 분획하여 간장 보호효과를 검색하였다. 영지버섯의 70% MeOH 추출물(GT)과 그 ether 분획(GE)에 대해 생쥐 1군을 6마리로 하여 20% $CCl_4$0.1$m\ell$/10g(olive oil로 희석)을 경구투여 하였다. 검액 GE는 50mg/kg과 250mg/kg, GT는 100mg/kg과 500mg/kg을 각각 사염화탄소 투여 30분 전에 경구투여 하였으며 사염화탄소를 투여하고 24시간 후에 심장 채혈하고 혈청을 분리하여 혈청성분 및 혈청효소의 활성을 측정하였다. 대조군에는 생리식염수를 투여하였고 양성 비교약물로는 silymarln 100mg/kg을 경구투여 하여 비교 관찰하였다. 실험 결과, 영지버섯의 ether 분획에서는 혈청중 GOT, GPT의 활성과 triglyceride의 함량에 대해 silymarin보다 우수한 효과를 보였으며, 70% MeOH 추출물은 silymarin에 미치지 못했다.

• Journal of Mushroom
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• v.12 no.4
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• pp.324-329
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• 2014

For evaluating the anti-allergy activity of mushrooms, forty two mushroom extracts were studied their inhibitory activities on the IgE-mediated degranulation in rat basophilic leukemia cell line (RBL-2H3 cell). Effects of mushroom extracts on the release of interleukin-4 (IL-4) and beta-hexosaminidase, and the cell viability of the IgE-sensitized were measured. From the analysis, five mushroom extracts such as the water extract from Flammulina velutipes (Curt.:Fr.) Sing showed the suppressive activities on IL-4 release as 20%. Eight extracts including the water extract of Ganoserma lucidum showed the suppressive activities on ${\beta}$-hexosaminidase release as 20%. Almost all of the extracts stimulated the proliferation of RBL-2H3 cell. The water extracts of Flammulina velutipes and Phellinus linteus were examined against the inhibitory activity in the production of IL-4 and ${\beta}$-hexosaminidase. Additionally, the extracts from Ganoserma lucidum, Isaria japonica, Phellinus linteus and Pleurotus ostreatus inhibited dose-dependently on ${\beta}$-hexosaminidase production. In conclusion, the result suggests that the mushrooms with the potent inhibitory efficacies on the degranulation of the mast cell would be candidate resources for the anti-allergy resources, and thus need to study for their utilization.

• Korean Journal of Food Science and Technology
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• v.39 no.4
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• pp.386-389
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• 2007

The antioxidant activities of Phellinus igniarius (PI) and Ganoderma lucidum (GL) extracts were evaluated after far-infrared (FIR) irradiation. PI and GL were irradiated at $120^{\circ}C$ for 30, 60, 90 and 120 min with a FIR heater (2-14 ${\mu}m$), and then extracted by distilled water for 1 hr. Total sugar contents (TSC), total phenolic contents (TPC), radical scavenging ability (RSA), and reducing power (RP) were significantly increased by FIR irradiation. When PI was irradiated for 120 min, the TSC, TPC, RSA, and RP values of the PI extracts increased from 10.1 to 25.9 mg/g, 6.1 to 13.4 mg/g, 23.8 to 51.5%, and 0.222 to 0.363, respectively, compared to the untreated controls. The GL extracts, under the same conditions ($120^{\circ}C$ for 120 min), also increased in TSC, TPC, RSA, and RP from 11.1 to 22.6 mg/g, 2.5 to 5.8 mg/g, 14.5 to 18.6%, and 0.271 to 0.296, respectively. These results indicate that appropriate FIR irradiation of PI and GL could enhance the antioxidant activities of their extracts by increasing amounts of phenolic and sugar compounds.