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http://dx.doi.org/10.14480/JM.2014.12.4.324

Study on the anti-allergy activity of mushrooms in IgE-sensitized RBL-2H3 cells  

Lee, Seung-Eun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Noh, Hyung-Jun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Choi, Jehun (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Kim, Geum-Sook (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Lee, Dae-Young (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Kim, Seung-Yu (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
Publication Information
Journal of Mushroom / v.12, no.4, 2014 , pp. 324-329 More about this Journal
Abstract
For evaluating the anti-allergy activity of mushrooms, forty two mushroom extracts were studied their inhibitory activities on the IgE-mediated degranulation in rat basophilic leukemia cell line (RBL-2H3 cell). Effects of mushroom extracts on the release of interleukin-4 (IL-4) and beta-hexosaminidase, and the cell viability of the IgE-sensitized were measured. From the analysis, five mushroom extracts such as the water extract from Flammulina velutipes (Curt.:Fr.) Sing showed the suppressive activities on IL-4 release as 20%. Eight extracts including the water extract of Ganoserma lucidum showed the suppressive activities on ${\beta}$-hexosaminidase release as 20%. Almost all of the extracts stimulated the proliferation of RBL-2H3 cell. The water extracts of Flammulina velutipes and Phellinus linteus were examined against the inhibitory activity in the production of IL-4 and ${\beta}$-hexosaminidase. Additionally, the extracts from Ganoserma lucidum, Isaria japonica, Phellinus linteus and Pleurotus ostreatus inhibited dose-dependently on ${\beta}$-hexosaminidase production. In conclusion, the result suggests that the mushrooms with the potent inhibitory efficacies on the degranulation of the mast cell would be candidate resources for the anti-allergy resources, and thus need to study for their utilization.
Keywords
Allergy; Beta-hexosaminidase; Degranulation; IL-4; mast cell;
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Times Cited By KSCI : 2  (Citation Analysis)
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