• Korean Journal of Clinical Laboratory Science
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• v.53 no.1
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• pp.49-59
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• 2021

This study investigated the use of real-time PCR melting curves for the diagnosis of blaKPC and blaNDM genes among the most frequently detected carbapenemase-producing Enterobacteriaceae in Korea. As a means of addressing the shortcomings of phenotype tests and conventional PCR. The modified Hodge test confirmed positivity in 25 of 35 strains, and carbapenemase inhibition testing confirmed positivity in 14 strains by meropenem+PBA or meropenem+EDTA. PCR analysis showed amplification products in 25 strains of Klebsiella pneumoniae carbapenemases (KPC), 10 of K. pneumoniae, 5 of E. coli, 5 of A. baumannii, 4 of P. aeruginosa, and 1 of P. putida. New Delhi metallo β-lactamase (NDM) identified amplification products in 8 strains, that is, 2 K. pneumoniae, 3 E. coli, 1 P. aeruginosa, 1 E. cloacae, and 1 P. retgeri strains. Real-time PCR melting curve analysis confirmed amplification in 25 strains of KPC and 8 strains of NDM, and these results were 100% consistent with PCR results. In conclusion, our findings suggest early diagnosis of carbapenem resistant Enterobacteriaceae by real-time PCR offers a potential means of antibacterial management that can prevent and control nosocomial infection spread.

• Korean Journal of Clinical Laboratory Science
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• v.54 no.3
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• pp.179-191
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• 2022

Carbapenem-resistant Enterobacteriaceae (CRE) poses an increasing public health threat and has limited treatment options with high associated mortality. Genotypes of carbapenemase that threaten public health (bla_KPC, bla_NDM, bla_IMP, and bla_VIM) and bla_OXA-48-like genes were detected by phenotypic and molecular diagnosis, and related gene distribution patterns were investigated. Phenotypic testing using the modified Hodge test confirmed positivity in all 41 strains examined, and carbapenemase inhibitory testing using meropenem+phenyl boronic acid or meropenem+EDTA confirmed positivity in 18 and 8 strains, respectively. Polymerase chain reaction revealed the presence of amplification products in 28 strains of bla_KPC, 25 strains of bla_NDM, 5 strains of bla_IMP, 1 strain of bla_VIM, and 13 bla_OXA-48-like strains. In addition, 7 strains of bla_KPC+bla_NDM, 1 strain of bla_KPC+bla_IMP, 1 strain of bla_NDM+bla_OXA-48-like, 1 strain of bla_NDM+bla_VIM, 4 strains of bla_KPC+bla_NDM+bla_IMP, and 4 strains of bla_KPC+bla_NDM+bla_OXA-48-like were identified. Melting curve analysis using real-time PCR was wholly consistent with PCR results. The study shows genetic identification of highly specific CRE by real-time PCR could be used to provide early diagnoses and infection control, improve surveillance, and prevent the transmission of CRE.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.4
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• pp.312-320
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• 2021

In this study, we aimed to develop a feed additive that can promote the growth of stone flounder (Kareius bicoloratus). To this end, we fermented mealworm medium (5%, w/v) with lactic acid bacteria (LAB) with antibacterial function (HeTm_LpWpPa) and evaluated its efficacy as a feed additive. Fish (approximately 5-6 g) were reared in six completely recirculating tanks (100 fish/tank) for 6 weeks. The total length of fish with and without HeTm_LpWpPa supplementation was 8.26 and 8.42 cm and body weight was 19.71 and 20.28 g, respectively, at 6 weeks. The weight gain of the control and experimental groups was 223.26 % and 248.76 %, respectively. The specific growth rate at 6 weeks was 2.79 % (control) and 2.98% (experimental), and the growth rate of the experimental group was slightly higher (0.19 %) than that of the control group. At the end of the experiment (i.e. 6 weeks), the number of fish below the mean weight in the experimental group was 3 (1 %) and that in the control group was 17 (5.7 %). These results indicate that feed supplementation of 10 % HeTm_LpWpPa could be utilized to promote the growth of flounder in farming.

• Journal of the Korean Association of Geographic Information Studies
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• v.25 no.1
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• pp.77-100
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• 2022

In the era of low growth, many provincial cities are experiencing population decline and aging. Population decline phenomena such as reduction of productive manpower, reduction of finances, deterioration of quality of life, and collapse of the community base are occurring in a chain and are being pushed to the brink of extinction of the cities. This study aims to propose a methodology to objectively estimate the employment creation centers and setting the basic unit of industrial-centered zoning by applying spatial statistical techniques and GIS for the application of the compact city plan as an efficient spatial management policy in a city with a declining population. In details, based on reviewing previous studies on compact city, 'employment complex index(ECI)' were defined considering the number of workers, the number of settlers, and the area of development land, the employment creation center was estimated by applying the 'Local Moran's I' and 'Getis-Ord's Hot-Spot Analysis'. As a case study, changes in the four years of 2013, 2015, 2017, and 2019 were compared and analyzed for Changwon City. As a result, it was confirmed that the employment creation center is becoming compacted and polycentric, which is a significant result that reflects the actual situation well. This results provide the basic data for functional and institutional territorial governance for the regional revitalization platform, and provide meaningful information necessary for spatial policy decision-making, such as population reduction, regional gross domestic product, and public facility arrangement that can respond to energy savings, transportation plans, and medical and health plans.

• Journal of fish pathology
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• v.35 no.2
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• pp.195-203
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• 2022

This study was performed to delineate pharmacokinetic residue characteristics of lincomycin (LM) in olive flounder at low water temperature (13℃), and to examine whether LM is effective against main Gram-negative pathogens. It was observed that the times for residue declined below the maximal residue limit (0.1 mg/kg) in the muscle was, respectively, 32, 33 and 55 days following 10, 20 and 40 mg/kg intramuscular LM injections. The in vitro MIC value of LM against Edwardsiella piscida was higher than 256 ㎍/mL and against Vibrio harveyi 32 ㎍/mL when examined with several clinical isolates. These results suggest that the withdrawal time of LM should be more than 30 days in consideration of the 10 mg/kg employed as the regular clinical dose. It was also found that LM administered to treat streptococosis in olive flounder is not likely to be effective against some pathogenic Gram-negative bacteria.

• Journal of Digital Convergence
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• v.20 no.5
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• pp.627-635
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• 2022

As the non-face-to-face situation continues for a long time due to COVID-19, the underlying technologies of the 4th industrial revolution such as IOT, AR, VR, and big data are affecting the metaverse platform overall. Such changes in the external environment such as society and culture can affect the development of academics, and it is very important to systematically organize existing achievements in preparation for changes. The Korea Educational Research Information Service (RISS) collected data including the 'metaverse platform' in the keyword and used the text mining technique, one of the big data analysis. The collected data were analyzed for word cloud frequency, connection strength between keywords, and semantic network analysis to examine the trends of metaverse platform research. As a result of the study, keywords appeared in the order of 'use', 'digital', 'technology', and 'education' in word cloud analysis. As a result of analyzing the connection strength (N-gram) between keywords, 'Edue→Tech' showed the highest connection strength and a total of three clusters of word chain clusters were derived. Detailed research areas were classified into five areas, including 'digital technology'. Considering the analysis results comprehensively, It seems necessary to discover and discuss more active research topics from the long-term perspective of developing a metaverse platform.

• Journal of the Society of Disaster Information
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• v.18 no.4
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• pp.861-872
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• 2022

The purpose of this study was to establish a complex disaster scenario that can comprehensively consider various disaster situations that may occur in the utility tunnel. Method: In order to comprehensively consider the correlation between disasters, a composite disaster scenario was derived from a combination of damage factors, respectively. A risk assessment was performed in order to derive the priorities of the scenarios. And based on the results, the priorities of complex disaster scenarios were set. Result: Based on the disaster cases in the utility tunnel, a plan was prepared for complex disaster scenarios centered on damage. A complex disaster scenario was specified using a semi-quantitative evaluation method for single and multiple disaster factors such as fire, flooding, and earthquake. Conclusion: The composite disaster scenario derived from this study can be used for the prevention and preparation of damage when the precursor symptoms of a disaster are detected. In addition, the results of this study are expected to be used as basic data for preparing strategic plans and preparing complex disaster response technologies to induce rapid response and recovery in case of emergency disasters.

• Journal of Digital Policy
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• v.2 no.3
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• pp.25-32
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• 2023

This study was done clarify the concept of clinical nurse's self-efficacy. This study was conducted using Walker & Avant's concept analysis framework, There were five aspects of the concept of Clinical Nurse's Self-Efficacy: To have faith and expectation that nurses can overcome stress and adversity in the clinical environment, To have a clear direction by believing that nurses themselves have the ability to perform nursing tasks, Allowing nurses to continue their work by believing that they have appropriate judgment to control various situations in clinical practice, Having a challenging sense of goal and motivating for the future through active participation in nursing work, and influencing various aspects from individual nurses to patients and hospital organizations. This conceptual analysis of clinical nurses' self-efficacy can recognize the need to prepare an educational system to improve nurses' self-efficacy and conduct related research.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2022.10a
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• pp.562-565
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• 2022

Recently, the increasing number of overloaded vehicles on the road poses a risk to traffic safety, such as falling objects, road damage, and chain collisions due to the abnormal weight distribution, and can cause great damage once an accident occurs. However, this irregular weight distribution is not possible to be recognized with the current weight measurement system for vehicles on roads. To address this limitation, we propose to build an object detection-based AI model to identify overloaded vehicles that cause such social problems. In addition, we present a simple yet effective method to construct an object detection model for the large-scale vehicle images. In particular, we utilize the large-scale of vehicle image sets provided by open AI-Hub, which include the overloaded vehicles from the CCTV, black box, and hand-held camera point of view. We inspected the specific features of sizes of vehicles and types of image sources, and pre-processed these images to train a deep learning-based object detection model. Finally, we demonstrated that the detection performance of the overloaded vehicle was improved by about 23% compared to the one using raw data. From the result, we believe that public big data can be utilized more efficiently and applied to the development of an object detection-based overloaded vehicle detection model.

• Journal of the Korea Convergence Society
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• v.13 no.1
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• pp.119-129
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• 2022

Cervical cytology has been widely used as a screening tool for cervical cancer. However, Human papillomavirus (HPV) detection and subtype testing are suggested to overcome the high false-negative rate associated with cytology. We aimed to investigate the clinical usefulness and infection rate in the HPV polymerase chain reaction (PCR) test performed in hospitals. HPV PCR data from 217 patients were analyzed. Analysis of variance revealed a significant difference in the infection rate among different age groups (P=0.015). The biopsy results showed that epithelial cell abnormalities and high HPV-positivity rate was observed in 1 (100%) subject aged <29 years, in 4 out of 5 (80%) patients in their 30s, and in 3 out of 4 (75%) patients aged ≥70 years. The prevalence of HPV infection was very high (46.1%). The highest prevalence (87.5%) was observed among patients in their <29, followed by those in their 30s (67.7%) and those in their 40s (31.9%).A high rate of epithelial cell abnormalities (≥ cervical intraepithelial neoplasia type 1, mild dysplasia) was observed in HPV-infected women aged<30 years. Therefore, extensive research and prevention activities are needed in this age group. HPV PCR testing is recommended to complement cervical cytology