• Journal of Life Science
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• v.29 no.10
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• pp.1144-1151
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• 2019

Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease associated with various metabolic syndromes, such as obesity, dyslipidemia, hypertension, and diabetes. Cudrania tricuspidata is a medicinal plant distributed widely in Asia and has been used in clinical practice to treat various diseases. The aim of this study is to determine the lipid-lowering effects of C. tricuspidata fruit extract (CTE) using a cell model induced by free fatty acids (FFAs). HepG2 cells were exposed to 1mM FFAs (palmitic acid:oleic acid = 2:1) for 24 hr to simulate the conditions of NAFLD in vitro. CTE attenuated the increases of lipid accumulation, intracellular triglyceride, and cholesterol content and inhibited 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) activity in the HepG2 cells in a dose-dependent manner. Also, CTE inhibited the protein expression of lipogenesis-related genes, such as sterol regulatory element-binding protein-1/-2 (SREBP-1/-2), fatty acid synthase (FAS), and stearoyl CoA desaturase-1 (SCD-1) in FFAs-induced lipid accumulation in HepG2 cells. In addition, CTE-induced adenosine monophosphate-activated protein kinase (AMPK) phosphorylation in HepG2 cells. These results suggest that CTE attenuates hepatic lipid accumulation by inhibiting lipogenesis through the modulation of the AMPK signaling pathway on FFAs-induced lipogenesis in HepG2 cells and may potentially prevent NAFLD.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.36-36
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• 2019

본 연구는 한라산국립공원 동사면에 위치한 구상나무숲 장기생태 모니터링 5ha 조사지역 ($20{\times}20m$, 125개 방형구)에서 구상나무의 유묘 및 치수 발생 현황을 파악하기 위하여 수행되었다. 조사는 2016년 6~8월 사이에 $5{\times}5m$ 방형구 125곳에 대해 계통추출법을 적용하여 실시하였다. 조사한 전체 125개 방형구 중에서 54개에서만 유묘가 관찰되었으며, 그 내용은 유묘 615개체 및 치수 1개체로 총 616개체가 발견되었다. 그러나 과반수의 71개 방형구에서 치수뿐만 아니라 유묘도 출현하지 않았다. 심지어 전체 125개 방형구에서 유묘가 가장 많이 관찰된 방형구(2-6지역, 95개체)를 제외하면, 나머지 124개 방형구의 평균 유묘 개체수는 4.2개로 매우 적었고 높이도 35cm 이하에 불과했다. 이와 함께 장기생태 모니터링 조사 지역 내에서 $1{\times}1m$ 방형구 183곳에 대해 단순 무작위 추출법으로 발생지 조사를 수행하였다. 발생지 조사 결과 유묘 2,518개체, 치수 2개체 및 유목 2개체 등 총 2,522개체가 발견되어 계통추출법을 적용한 조사와 유사한 결과가 나타났다. 본 연구를 통해 한라산국립공원 동사면 구상나무숲 내에서 구상나무 유묘의 발생은 어느 정도 이루어지고 있으나, 교란으로 인하여 치수로의 발달이 제한되는 것으로 판단된다. 여러 교란 요인 중에서 조사된 총 3,138개체 중 12.1%, 381개체에서 유제류인 노루에 의한 섭식이 확인되어 노루의 섭식이 유묘 발생 및 치수 발달에 교란을 야기하는 가장 중요한 요인으로 추정해볼 수 있다. 한반도 특산식물 구상나무의 보전 대책 수립을 위한 유묘 및 치수 교란 원인에 대한 지속적인 모니터링이 진행되어야 한다고 사료된다.

• Journal of Marine Life Science
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• v.6 no.2
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• pp.58-65
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• 2021

As aging progresses, reactive oxygen species (ROS) reduces skin moisturization and collapses skin barrier function. In this study, we evaluated the efficacy of skin moisturizing and skin barrier function enhancement by extracts from halophytes using HaCaT cells. Spartina anglica (S. anglica; SAE) and Calystegia soldanella (C. soldanella; CSE), a kind of halophytes, were collected from Dongmak beach in Incheon, and extracted with 70% ethanol. At the first, we evaluated the cytotoxicity of extracts in HaCaT cell using WST-8 Kit. As a result, the other experiment was conducted by setting the concentration at which the cell viability was 90% or more. SAE and CSE showed high radical scavenging activity through ABTS assay. Expression levels of genes related to skin moisturizing and skin barrier functions, were analyzed by real-time qPCR. As a result, it showed that the expression of aquaporin 3, hyaluronan synthase 2, and transglutaminase 1 was increased by SAE treatment but not changed by CSE. Activation of extracellular signal regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 mitogen activated protein kinase was induced by SAE. These results suggest that SAE can be used as functional materials for cosmetics for skin moisturizing and barrier function enhancement.

• Journal of Life Science
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• v.32 no.1
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• pp.51-55
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• 2022

Matrix metalloproteinase (MMP) enzymes are responsible for the degradation and formation of the extracellular matrix (ECM), and overproduction of MMPs is observed in several diseases, such as cancer and asthma, that progress with metastatic characteristics. Natural products, especially phytochemicals, have been an important source of MMP inhibitors with reduced side effects. Although the majority of phytochemicals inhibit the enzymatic activity of MMPs, some suppress MMP production. In this context, the current study evaluated the potential of Corydalis heterocarpa, a halophyte with reported bioactivities, to inhibit MMP expression in PMA-stimulated HT-1080 cells. A crude C. heterocarpa extract was shown to decrease the mRNA and protein expression of MMP-2 and MMP-9 while increasing the endogenous MMP inhibitors TIMP-1 and TIMP-2 which regulate MMP expression in healthy tissues. In addition, our results show that the inhibitory effects of C. heterocarpa might occur through suppression of the phosphorylation of MAPK signaling, the upstream activator of MMP overexpression. In conclusion, C. heterocarpa is a potential source of antimetastatic compounds that might serve as lead molecules to develop novel MMP inhibitors.

• The Journal of the Acoustical Society of Korea
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• v.43 no.1
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• pp.78-88
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• 2024

Ship-radiated noise received by passive sonar that can measure underwater noise can be identified and classified ship using Detection of Envelope Modulation on Noise (DEMON) analysis. However, in a low Signal-to-Noise Ratio (SNR) environment, it is difficult to analyze and identify the target frequency line containing ship information in the DEMONgram. In this paper, we conducted a study to extract target frequency lines using semantic segmentation among deep learning techniques for more accurate target identification in a low SNR environment. The semantic segmentation models U-Net, UNet++, and DeepLabv3+ were trained and evaluated using simulated DEMONgram data generated by changing SNR and fundamental frequency, and the DEMONgram prediction performance of DeepShip, a dataset of ship-radiated noise recordings on the strait of Georgia in Canada, was compared using the trained models. As a result of evaluating the trained model with the simulated DEMONgram, it was confirmed that U-Net had the highest performance and that it was possible to extract the target frequency line of the DEMONgram made by DeepShip to some extent.

• Journal of Life Science
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• v.27 no.12
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• pp.1437-1444
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• 2017

Sargassum macrocarpum is a widely distributed marine brown algae found in the North Pacific. The objective of this study was to evaluate the anti-inflammatory activity of an ethanol extract of S. macrocarpum (EESM). First, we investigated the anti-inflammatory activities of EESM in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. EESM treatment suppressed nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) production and inhibited the expressions of the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the mRNA and protein levels. In addition, the expression of pro-inflammatory cytokines, such as tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) and interleukin-1 beta ($IL-1{\beta}$), was decreased in a dose dependent manner. Investigation of the signaling pathways of nuclear factor kappa B ($NF-{\kappa}B$), phosphoinositide-3-kinase (PI3K)/Akt, and mitogen-activated protein kinases (MAPKs) revealed suppression of $NF-{\kappa}B$ translocation from the cytosol to nucleus by EESM treatment. The phosphorylation of the Akt and ERK proteins was also inhibited by EESM treatment. EESM treatment also stimulated the expression of the heme oxygenase-1 (HO-1) enzyme and its upstream transcription factor, nuclear factor-E2-related factor 2 (Nrf2). These results suggest that EESM has anti-inflammatory activity and could have potential uses in the field of nutraceuticals.

• Science of Emotion and Sensibility
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• v.15 no.2
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• pp.231-238
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• 2012

This study aims for building an application system of converting sound into color image based on synesthetic perception. As the major features of input sound, both scale and octave elements extracted from F0(fundamental frequency) were converted into both hue and intensity elements of HSI color model, respectively. In this paper, we used the fixed saturation value as 0.5. On the basis of color model conversion theory, the HSI color model was then converted into the RGB model, so that a color image of the BMP format was finally created. In experiments, the basic system was implemented on both software and hardware(TMS320C6713 DSP) platforms based on the proposed sound-color image conversion method. The results revealed that diverse color images with different hues and intensities were created depending on scales and octaves extracted from the F0 of input sound signals. The outputs on the hardware platform were also identical to those on the software platform.

• Journal of Digital Contents Society
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• v.19 no.6
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• pp.1207-1212
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• 2018

The ultrasound image uses ultrasonic pulses to receive the reflected waves and construct an image necessary for diagnosis. At this time, when the signal becomes weak, noise is generated and a slight difference in brightness occurs. In addition, fluctuation of image due to breathing phenomenon, which is the characteristic of ultrasound image, and change of motion in real time occurs. Such a noise is difficult to recognize and diagnose visually in the analysis process. In this paper, morphological features are automatically extracted by using image processing technique on ultrasound acquired images. In this paper, we implemented a GPU - based fast filter using a cloud big data processing platform for image processing. In applying the GPU - based high - performance filter, the algorithm was run with performance 4.7 times faster than CPU - based and the PSNR was 37.2dB, which is very similar to the original.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.47 no.8
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• pp.61-67
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• 2010

A characterization procedure for analyzing symmetric coupled MIS(Metal-Insulator-Semiconductor) transmission line is used the same procedure as a general single layer symmetric coupled line with perfect dielectric substrate from the extraction of the characteristic impedance and propagation constant for even- and odd-mode. In this paper, an analysis for a new substrate shielding symmetric coupled MIS structure consisting of grounded crossbar at the interface between Si and SiO2 layer using the Finite-Difference Time-Domain (FDTD) method is presented. In order to reduce the substrate effects on the transmission line characteristics, a shielding structure consisting of grounded crossbar lines over time-domain signal has been examined. Symmetric coupled MIS transmission line parameters for even- and odd-mode are investigated as the functions of frequency, and the extracted distributed frequency-dependent transmission line parameters and corresponding equivalent circuit parameters as well as quality factor for the new MIS crossbar embedded structure are also presented. It is shown that the quality factor of the symmetric coupled transmission line can be improved without significant change in the characteristic impedance and effective dielectric constant.

• Journal of IKEEE
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• v.13 no.2
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• pp.118-125
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• 2009

With recent advancement of high-speed, multi-gigabit data transmission capabilities, transmitters usually send data without clock signals for reduction of hardware complexity, power consumption, and cost. Therefore clock and data recovery circuits(CDR) become important to recover the clock and data signals and have been widely studied. This paper presents the design of 10Gbps CDR in 0.18$\mu$m CMOS process. A quarter-rate bang-bang phase detector is designed to reduce the power and circuit complexity, and a 4-stage LC-type VCO is used to improve the jitter characteristics. Simulation results show that the designed CDR consumes 80mW from a 1.8V supply, and exhibits a peak-to-peak jitter of 2.2ps in the recovered clock. The chip layout area excluding pads is 1.26mm$\times$1.05mm.